RESUMO
OBJECTIVES: Describing the behaviour of the A H1N1/09 virus related to time, age and gender in patients having suspected infection, medical health care and laboratory diagnosis. METHOD: This was a descriptive and retrospective study of patients diagnosed as having the influenza A H1N1/09 virus between April 2009 and July 2010 by the Bogotá Public Health Laboratory. RESULTS: The first cases of A H1N1/09 virus were confirmed since week 17, 2009; positivity increased gradually, reaching maximum expression between weeks 31-36, 2009 (43 % to 53 %) and decreased during the 37th week. The age groups most affected were 6-15 years (35.4 %) and 16-25 years (28 %) (p=0.0044); the lowest percentages were found in children aged less than 1 year (8.7 %) and people older than 65 years (7.2 %) (Chi 1.98, p=0.119). The gender ratio was similar: female (18.6 %) and male (17.6 %) (Chi 1.82, p=0.1768). CONCLUSIONS: There was a significant increase in influenza A cases during 2009. However, this did not alter the behaviour of the endemic respiratory syncytial virus; on the other hand, the H1N1/09 subtype replaced the seasonal virus circulating amongst the population of Bogotá, similarly affecting men and women, mainly young adults. The highest prevalence of cases occurred between August and September 2009.
Assuntos
Vírus da Influenza A Subtipo H1N1 , Influenza Humana/epidemiologia , Pandemias , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Colômbia/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Vigilância da População , Prevalência , Infecções por Vírus Respiratório Sincicial/epidemiologia , Estudos Retrospectivos , Estações do Ano , Adulto JovemRESUMO
Objetivo Describir el comportamiento del virus de Influenza A H1N1/09 según variables de tiempo, edad y sexo, en pacientes con sospecha de infección, atención médica y diagnóstico por laboratorio. Método Estudio descriptivo retrospectivo de pacientes diagnosticados con Influenza A H1N1/09 entre abril 2009 y julio de 2010 en el Laboratorio de salud Pública de Bogotá D.C. Resultados Se confirmaron los primeros casos de Influenza A H1N1/09 desde la semana 17 de 2009; a continuación la positividad creció gradualmente, hasta manifestar su máxima expresión entre las semanas 31 a 36 de 2009 (43 por ciento a 53 por ciento), descendiendo a partir de la semana 37. Los grupos de edad más afectados fueron los de 6 a 15 años (35,4 por ciento) y de 16 a 25 años (28 por ciento) (p=0.0044); las proporciones más bajas se hallaron en menores de 1 año (8,7 por ciento) y mayores de 65 años (7,2 por ciento) (Chi 1,98, p=0.119). La proporción por sexo fue similar: femenino (18,6 por ciento) y masculino (17,6 por ciento) (Chi 1,82, p=0.1768). Conclusiones Durante el año 2009, el virus Influenza A presentó un aumento significativo de casos; sin embargo, no modificó el comportamiento endémico del virus Sincitial Respiratorio. Por otra parte, el subtipo H1N1/09 reemplazó casi en su totalidad al virus estacional que tradicionalmente circulaba entre la población Bogotana y afectó en forma similar a hombres y mujeres, principalmente adultos jóvenes. El mayor predominio de casos se presentó entre los meses de agosto y septiembre de 2009.
Objectives Describing the behaviour of the A H1N1/09 virus related to time, age and gender in patients having suspected infection, medical health care and laboratory diagnosis. Method This was a descriptive and retrospective study of patients diagnosed as having the influenza A H1N1/09 virus between April 2009 and July 2010 by the Bogotá Public Health Laboratory. Results The first cases of A H1N1/09 virus were confirmed since week 17, 2009; positivity increased gradually, reaching maximum expression between weeks 31-36, 2009 (43 percent to 53 percent) and decreased during the 37th week. The age groups most affected were 6-15 years (35.4 percent) and 16-25 years (28 percent) (p=0.0044); the lowest percentages were found in children aged less than 1 year (8.7 percent) and people older than 65 years (7.2 percent) (Chi 1.98, p=0.119). The gender ratio was similar: female (18.6 percent) and male (17.6 percent) (Chi 1.82, p=0.1768). Conclusions There was a significant increase in influenza A cases during 2009. However, this did not alter the behaviour of the endemic respiratory syncytial virus; on the other hand, the H1N1/09 subtype replaced the seasonal virus circulating amongst the population of Bogotá, similarly affecting men and women, mainly young adults. The highest prevalence of cases occurred between August and September 2009.
Assuntos
Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Vírus da Influenza A Subtipo H1N1 , Influenza Humana/epidemiologia , Pandemias , Colômbia/epidemiologia , Vigilância da População , Prevalência , Infecções por Vírus Respiratório Sincicial/epidemiologia , Estudos Retrospectivos , Estações do AnoRESUMO
A wide variety of in vitro models have been used for studying rabies infection, however, currently, no central nervous system (CNS) adult neuron cultures are available. The current study determined the susceptibility to rabies infection in an adult CNS neuron cell line (CAD-R1). Cultures of CAD-R1 cells were held for 5 days in medium containing serum (undifferentiated CAD-R1 cells) or in serum-free medium (differentiated CAD-R1 cells). They were then infected with highly neurotropic rabies virus (RV) strain (CVS), obtained from fibroblastic cells (CVS-BHK) or from adult mouse brain (CVS-MB). Undifferentiated and differentiated cells were infected with the two RV strains, but the percentage of infected cells in differentiated cultures was significantly greater (83% and 79%, respectively) than in undifferentiated cells (51% and 60%) (Student's t test<0.05). Susceptibility to infection apparently depended on cellular differentiation state, possibly due to acquisition of additional morphological and biochemical characteristics during the differentiation process that made them more susceptible to RV infection. Therefore, CAD R1 cells may represent a good model for RV infection, making them a useful tool for studying RV neurotropism, infection pathogeny, isolation of street virus or producing safer and most potent vaccines.