RESUMO
Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity.
Assuntos
Antígenos de Helmintos/imunologia , Proteínas de Helminto/imunologia , Filogenia , Schistosoma mansoni/química , Alérgenos/classificação , Alérgenos/genética , Alérgenos/imunologia , Animais , Antígenos de Helmintos/classificação , Antígenos de Helmintos/genética , Western Blotting , Reações Cruzadas , Vacinas contra Dengue/imunologia , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Perfilação da Expressão Gênica , Proteínas de Helminto/classificação , Proteínas de Helminto/genética , Soros Imunes/imunologia , Espectrometria de Massas , Família Multigênica , Schistosoma mansoni/classificação , Schistosoma mansoni/genética , Schistosoma mansoni/imunologia , Transcrição GênicaRESUMO
Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A–D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity.