RESUMO
Approximately 20% industrial water pollution comes from textile dyeing process, with Azo dyes being a major problem in this scenario and requiring new forms of efficient treatment. Effluent treatments using the Advanced Oxidation Processes (AOP) are justified by the potential of application in the dyed effluent treatments once they can change the Azo dye chemical structure. Thus, this study aimed to evaluate the toxicity and mutagenic capacity of a synthetic effluent containing Amido Black 10B (AB10B) azo dye before treatment with AOP, named Gross Synthetic Effluent (GSE), and after the AOP, named Treated Synthetic Effluent (TSE). Daphnia magna and Allium cepa tests were used to evaluate acute toxicity effects and chromosomal mutagenesis, respectively. The Salmonella/microsome assay was performed to evaluate gene mutations. In silico assays were also performed aiming to identify the mutagenic and carcinogenic potential of the degradation byproducts of AB10B. There was 100% immobility to D. magna after 24 h and 48 h of treatments with TSE, showing EC50 values around 5%, whereas GSE did not show acute toxicity. However, GSE induced chromosomal mutations in A. cepa test. Both GSE and TSE were not able to induce gene mutations in S. typhimurium strains. These effects can be associated with two byproducts generated with the cleavage of the azo bonds of AB10B, 4-nitroaniline and -2-7-triamino-8-hydroxy-3-6-naphthalinedisulfate (TAHNDS). In conclusion, AOP is an efficient method to reduce the mutagenicity of synthetic effluent containing AB10B and additional methods should be applied aiming to reduce the toxicity.
Assuntos
Mutagênicos , Poluentes Químicos da Água , Animais , Compostos Azo/toxicidade , Corantes/toxicidade , Daphnia , Mutagênese , Testes de Mutagenicidade , Mutagênicos/análise , Mutagênicos/toxicidade , Indústria Têxtil , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
This paper reports results of genotoxicity and toxicity studies of water and sediment samples collected from the Estância Velha stream of southern Brazil, a stream transporting both domestic sewage and effluents from regional factories working in the leather industry. Three sites were selected: in the stream headwaters (Site 1), located downstream of an urban area (Site 2), and near the basin outfall (Site 3). Results obtained with Allium cepa showed no evidence of chromosomal mutation, either in water or in sediment, during winter or summer seasons, but samples collected below Site 1 showed high toxicity. Physical and chemical analyses showed high concentrations of pollutants at these sites. Ecotoxicity tests with Daphnia magna and Ceriodaphnia dubia measured toxicity in water from Sites 2 and 3 in summer 2004. A toxic effect on Hyalella azteca was only found in sediment from Site 3 during winter 2003 and summer 2004. The results suggest that the synergy among different compounds in domestic and industrial sewage discharges can make it difficult to maintain system stability.