Assuntos
Linfócitos B/imunologia , Imunoglobulina E/biossíntese , Linfonodos/imunologia , Linfócitos/imunologia , Infecções por Nematoides/imunologia , Animais , Células da Medula Óssea , Células Cultivadas , Feminino , Ativação Linfocitária , Nippostrongylus , Ratos , Receptores de Antígenos de Linfócitos B , Baço/imunologiaRESUMO
Normal rat bone marrow cells incubated with serum or lymph from Nippostrongylus brasiliensis (Nb)-infected rats showed an increase in the proportion of IgE-bearing cells in culture. This effect was produced in a similar fashion by cell-free supernatants (CFS) from cultures of mesenteric lymph node cells obtained from Nb-infected rats. The action of CFS on bone marrow cells appeared to be specific for the generation of IgE-bearing cells since the proportion of IgM-bearing cells in the culture did not change. The IgE-bearing cells in bone marrow cell cultures consisted of small lymphocytes, blast cells, and mast cells, and the addition of CFS to the cultures predominantly increased the number of IgE-bearing blast cells. CFS was also effective in increasing the proportion of IgE-bearing small lymphocytes in cultures of normal mesenteric lymph node cells. Removal of IgE in CFS by an anti-IgE immunosorbent did not affect the ability of CFS to generate IgE-bearing cells. The factor(s) in CFS responsible for this activity was shown to migrate with serum beta-globulins in zone electrophoresis and to possess a molecular size of between 10(4) and 2 X 10(4) m.w. The ability of CFS to generate IgE-bearing cells was diminished by treatment with the enzymes trypsin and ribonuclease A, but was unaffected by chymotrypsin.
Assuntos
Imunoglobulina E/biossíntese , Linfócitos/imunologia , Infecções por Nematoides/imunologia , Animais , Linfócitos B/imunologia , Medula Óssea/imunologia , Células da Medula Óssea , Células Cultivadas , Meios de Cultura , Feminino , Lipopolissacarídeos/farmacologia , Linfa/imunologia , Linfonodos/imunologia , Ativação Linfocitária , Macrófagos/imunologia , Nippostrongylus , Ratos , SolubilidadeAssuntos
Ancylostomatoidea , Células Produtoras de Anticorpos/citologia , Imunoglobulina E/biossíntese , Nippostrongylus , Receptores de Antígenos de Linfócitos B/análise , Tricostrongiloidíase/etiologia , Animais , Especificidade de Anticorpos , Células Produtoras de Anticorpos/imunologia , Diferenciação Celular , Feminino , Imunofluorescência , Imunoglobulina E/análise , Linfonodos/imunologia , Linfócitos/imunologia , Ratos , Fatores de TempoRESUMO
The concentration of IgE in the serum of Sprague-Dawley rats increased after infection with Nippostrongylus brasiliensis (NB). The IgE concentration in normal rats was less than 1 mug/ml. After re-infection with NB, the concentration increased in 100 to 300 mug/ml. Mast cells were purified from peritoneal cells of both normal and NB-infected animals. Purified mast cells from the infected animals released histamine upon exposure to NB antigen. The antibody specific for IgE released histamine from purified mast cells of both normal and infected animals. Dose-reponse curves of histamine release suggested that mast cells from NB-infected animals bear more IgE molecules than normal mast cells. Binding of 125I-labeled rat E myeloma protein with normal mast cells was demonstrated by autoradiography. Under the same experimental conditions, mast cells of infected animals were not labeled with 125I-IgE. Mast cells from both normal and infected animals failed to combine 125I-labeled IgG. The number of IgE molecules bound per mast cell was determined by incubating 125I-labeled IgE with purified mast cells. When mast cells were incubated incubated in 0.6 to 2 mug/ml of IgE, the number of IgE molecules combined with the mast cells from infected animals was about 10% of that bound with normal mast cells. The results indicated that a large proportion of IgE receptors on mast cells of infected animals was occupied by their own IgE. No significant difference was observed between normal mast cells and those of infected animals with respect to histamine content and intracellular levels of cyclic nucleotides.