RESUMO
UNLABELLED: Epstein-Barr virus (EBV) is a well-known carcinogenic virus, and the association of EBV with some tumours suggests that there may also be an association between laryngeal carcinoma and EBV. OBJECTIVE: The aim of this study is to determine the role of EBV in the aetiology of laryngeal carcinoma. METHOD: Prospective investigation the EBV with real time polymerase chain reaction in tumour tissues of 25 patients with laryngeal carcinoma and 17 patients with benign laryngeal lesions, and investigation of the relationship between the presence of viral DNA and patients' smoking habits, alcohol consumption, localization and differentiation of the tumour. RESULTS: There was no significant difference between the control group and patient group in terms of EBV polymerase chain reaction positivity (p > 0.05). Also we couldn't find a statistically significant relationship between EBV positivity and differentiation of the tumour, localization of the tumour, smoking and alcohol consumption habits (p > 0.05). CONCLUSION: Our results suggest that, although EBV is present in some of the squamous cell laryngeal carcinomas, its presence has no effect on the pathogenesis of laryngeal carcinomas.
Assuntos
Carcinoma de Células Escamosas/virologia , DNA Viral/análise , Infecções por Vírus Epstein-Barr/complicações , Herpesvirus Humano 4/genética , Neoplasias Laríngeas/virologia , Adulto , Idoso , Consumo de Bebidas Alcoólicas/efeitos adversos , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Herpesvirus Humano 4/isolamento & purificação , Humanos , Neoplasias Laríngeas/patologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Risco , Fumar/efeitos adversosRESUMO
O vírus Epstein-Barr (EBV) é um conhecido vírus carcinogênico. A associação entre EBV e alguns tumores sugere que também pode haver correlação entre carcinoma de laringe e EBV. OBJETIVO: O presente estudo pretende determinar o papel do EBV na etiologia do carcinoma de laringe. MÉTODO: Estudo prospectivo sobre EBV por reação em cadeia da polimerase em tempo real em tecidos tumorais de 25 pacientes com carcinoma de laringe e 17 pacientes com lesões benignas de laringe; análise da relação entre presença de DNA viral e tabagismo, etilismo, localização e diferenciação tumoral. RESULTADOS: Não houve diferenças significativas entre os grupos de controle e de estudo para positividade da PCR para EBV (p > 0,05). Não foi identificada relação estatisticamente significativa entre positividade para EBV e diferenciação tumoral, localização da neoplasia, tabagismo ou etilismo (p > 0,05). CONCLUSÃO: Nossos resultados sugerem que, a despeito de sua identificação em alguns carcinomas espinocelulares de laringe, a presença de EBV não teve qualquer influência na patogenia do carcinoma de laringe.
Epstein-Barr virus (EBV) is a well-known carcinogenic virus, and the association of EBV with some tumours suggests that there may also be an association between laryngeal carcinoma and EBV. OBJECTIVE: The aim of this study is to determine the role of EBV in the aetiology of laryngeal carcinoma. METHOD: Prospective investigation the EBV with real time polymerase chain reaction in tumour tissues of 25 patients with laryngeal carcinoma and 17 patients with benign laryngeal lesions, and investigation of the relationship between the presence of viral DNA and patients' smoking habits, alcohol consumption, localization and differentiation of the tumour. RESULTS: There was no significant difference between the control group and patient group in terms of EBV polymerase chain reaction positivity (p > 0.05). Also we couldn't find a statistically significant relationship between EBV positivity and differentiation of the tumour, localization of the tumour, smoking and alcohol consumption habits (p > 0.05). CONCLUSION: Our results suggest that, although EBV is present in some of the squamous cell laryngeal carcinomas, its presence has no effect on the pathogenesis of laryngeal carcinomas.