RESUMO

Successive Negative Contrast (SNC) occurs when there is a reduction in the quantity or quality of a reward that is expected according to the presence of contextual cues. This induces an emotional response of frustration that is similar to stress. While this phenomenon has been observed in several mammal species, findings in domestic dogs have been inconsistent, although this issue has strong relevance in dog training. The aim of this study was to assess the effects of Successive Negative Contrast in two responses that had already been studied in this species, but with an increase in the methodological rigor and variations in the experimental conditions to examine the generalizability of the phenomenon. To this end, experimental dogs experienced a pre-shift phase in which they received a high-value reward (liver), followed by a post-shift phase in which they obtained a low-value reward (dry dog food), and then a re-shift phase in which the high-value reward was available again. Control dogs received dry food in all phases. The results show a contrast effect on the behavior of following human pointing to obtain food (Study 1). On the contrary, there were no differences in problem solving behavior after the de- and re-evaluation of the reward during a non-social task (Study 2). The results support that Successive Negative Contrast is not a consistent phenomenon in pet dogs. It is possible that certain characteristics of dogs such as the great availability of high-value rewards in their daily lives could attenuate the effects of a reduction in incentive value.

RESUMO

El sebaceoma es un tumor cutáneo poco frecuente que presenta diferenciación sebácea, y se localiza en piel de cabeza y cuello. Puede asociarse con la presencia de tumores malignos, asociación conocida como síndrome de Muir-Torre, por lo que ante su diagnóstico se deben descartar. Su localización en la piel del conducto auditivo externo es muy infrecuente. Presentamos nuestra experiencia en el manejo de esta patología en un varón de 78 años de edad que presentaba una lesión nodular sólida en el conducto auditivo externo izquierdo con pérdida de audición y otorrea como síntomas asociados. Se recomienda la exéresis completa de la lesión, como tratamiento de elección con fines tanto diagnósticos como terapéuticos.

Sebaceoma is a rare benign cutaneous tumor with sebaceous differentiation and it is typically located on the skin of the head and neck. This pathology made appear in association with malignant tumors (known as Muir-Torre syndrome) and must be ruled out. The location in the external auditory canal is very unusual. We present our experience in managing this pathology in a 78-year-old man who complains of hearing loss and otorrhea and presents a solid nodule in the left external auditory canal. Complete surgical removal was performed, as the choice treatment for diagnosis and therapeutic care.

Assuntos

RESUMO

La atresia de coana es una rara malformación congénita improbable de encontrar de forma bilateral en un adolescente o adulto. Hasta la fecha, no se ha descrito ningún caso de atresia bilateral en un adulto con una malformación asociada de cabeza y cuello que haya requerido tratamiento conjunto. El tratamiento de elección de la atresia de coana bilateral continúa siendo la cirugía endoscópica, con controversia en el uso intraoperatorio de mitomicina o la colocación de stents para evitar estenosis. Lo que no está claro es el orden de tratamiento y la simultaneidad del procedimiento si se asocian otras posibles patologías que tengan una indicación quirúrgica. Presentamos un caso clínico que cumple con todos estos requisitos.

Choanal atresia is a rare congenital malformation that is unlikely to be found bilaterally and is seldom diagnosed in adulthood. To date, no clinical case of bilateral atresia has been described with a head and neck malformation that requires surgical treatment in an adult. The preferred treatment is still endoscopic sinonasal surgery with discrepancies of the use or not of intraoperative topical mitomycin or the placement of stents to avoid restenosis. What is not clear is the order of treatment and simultaneity of the procedure with other possible associated pathologies that have a surgical indication. We present a clinical case that meets all these requirements.

Assuntos

RESUMO

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used due to it selective action, and preferential control of dicotyledonous weeds affecting cereal crops. Physiological responses of sensitive dicotyledonous plants to 2,4-D include growth retardation, senescence, and cell death. Due to soil and water contamination by agricultural practices, 2,4-D constitutes a potential risk to non-target plant species. In this work, the potential advantage of using organic modified bentonite (Bent) to adsorb 2,4-D and therefore mitigate damage produced by this herbicide on sensitive not-target vegetable species was investigated. Dodecylamine (DDA) was used as an organic modifier to change the hydrophilic nature of Bent into an organophilic matrix. The adsorption performances of 2,4-D by Bent-DDA were analyzed. The maximum adsorptions of 2,4-D (22.1 mg/L) from aqueous solution containing 1.0 or 2.5 mg/mL Bent-DDA were 40 and 80 %, respectively. The physical interaction of Bent-DDA with 2,4-D was characterized by Wide Angle X-ray Scattering (WAXS) and thermogravimetric analysis (TGA). The biological functionality of Bent-DDA matrix as 2,4-D adsorbent was tested in a bioassay in the Arabidopsis thaliana plant model system. The primary root growth of Arabidopsis seedlings is strongly inhibited by low concentrations of 2,4-D. Arabidopsis seedlings submitted to Bent-DDA pre-treated herbicide aqueous solution showed similar root growth than 2,4-D non-treated seedlings. Finally, the ability of Bent-DDA to prevent 2,4-D phytotoxicity was exploratory investigated in lettuce plants. Lettuce plants pre-treated with 20 µg/mL Bent-DDA showed reduced sensitivity to 2,4-D including an increment on chlorophyll content and biomass compared with non-treated plants. Our findings revealed a promising scenario for the application of Bent-DDA as an effective adsorbent of 2,4-D at productive scale.

Assuntos

RESUMO

El tratamiento ortodóncico permite rehabilitar la función como también la estética facial y de la sonrisa. En muchos casos, esto puede realizarse sin la utilización de brackets. Las placas alineadoras realizan movimientos en las arcadas dentarias y las posibilidades y los resultados obtenidos cada vez son mejores. La utilización del sistema de alineación con placas permite que muchos pacientes, que no están dispuestos a utilizar brackets, encuentren una respuesta a sus problemas de oclusión (AU)

Assuntos

RESUMO

AIMS: The aim of this study was to evaluate the microbiological quality of commercially prepared ready-to-eat (RTE) sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella ssp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form biofilms on stainless steel. METHODS AND RESULTS: There were four sampling events in seven establishments, totalling 28 pools of sushi samples. Mean AMB counts ranged between 5·2 and 7·7 log CFU per gram. The enumeration of TC varied between 2·1 and 2·7 log MPN per gram. Salmonella ssp. were not detected, and one sample was positive for E. coli and was identified as E. coli O157:H7. To the best of our knowledge, this is the first report of E. coli O157:H7 in sushi samples in the world literature. This isolate presented virulence genes stx1, stx2, eae and hlyA. It was also susceptible to 14 antimicrobials tested and had the ability to form biofilms on stainless steel. CONCLUSIONS: There is a need to improve the good hygiene practices adopted in establishments selling sushi in the city of Pelotas, Brazil. In addition, the isolated E. coli O157:H7 carries a range of important virulence genes being a potential risk to consumer health, as sushi is a RTE food. This isolate also presents biofilm formation ability, therefore, may trigger a constant source of contamination in the production line of this food. SIGNIFICANCE AND IMPACT OF THE STUDY: The increase in the consumption of sushi worldwide attracts attention regarding the microbiological point of view, since it is a ready-to-eat food. To our knowledge, this was the first time that E. coli O157:H7 was identified in sushi samples.

Assuntos

RESUMO

El objetivo fue comparar la rugosidad de un composite nanoparticulado con diferentes técnicas de acabado y pulido. Se obtuvieron 20 discos en conformadores ad-hoc en los que se insertó un composite nanoparticulado en capas de 2mm. Se activó cada incremento durante 30s con una intensidad de 1450 mw/cm2. Todas las probetas se pulieron con la secuencia completa de discos Sof-lex a velocidad media y luego se asignaron a uno de 4 grupos (n=5) tratados de la siguiente manera: G1: sin tratamiento extra; G2: con cepillos Astrobrush y JC-SICRA-G; G3: con pasta de diamantes Diamond Gloss aplicada con fieltro; y pasta de óxido de aluminio Poligloss y fieltro; G4: igual que G3 + cepillos (igual que G2). Las superficies se evaluaron con un perfilómetro óptico tridimensional. Se registró la rugosidad promedio (Ra) en tres puntos de cada espécimen, se consideró la Ra a la media de los tres registros obtenidos. Los datos se analizaron mediante ANOVA de una vía y prueba de Tukey para comparaciones múltiples. Los resultados obtenidos fueron: G1: 132,20nm (26,16); G2: 77,20 nm (20,64); G3: 78,74 nm (10,04); G4: 66,47nm (31,08). Se encontró diferencia estadísticamente significativa entre los grupos (P=0.0017); de las comparaciones múltiples surge que G1 presentó una rugosidad media significativamente mayor que los restantes. G1>G2 (P<0.01), G1>G3 (P<0,05), G1>G4 (P<0,01). En conclusión, el uso de sistemas anexos de pulido permite lograr mayor lisura superficial. El uso de más de un sistema complementario no mejora el resultado (AU)

Assuntos

RESUMO

BACKGROUND: Reconstructive joint surgery is an indicator of poor prognosis in rheumatoid arthritis (RA). Objectives of this study were to describe the incidence rate of orthopedic and hand surgery indication (OHSI) in an ongoing cohort of Hispanic early RA patients treated according to a T2T strategy and to investigate predictors. METHODS: Through February 2018, the cohort comprised 185 patients recruited from 2004 onwards, with variable follow-up, and rheumatic assessments at fixed intervals that included prospective determination of OHSI. Charts were reviewed by a single data abstractor. OHSI incidence rate was calculated. A case-control study nested within a cohort investigated the predictors; cases (OHSI patients) were paired with controls (1:4) according to age, sex and autoantibodies. A logistic regression model included baseline and cumulative (up to OHSI or equivalent) variables related to disease activity, treatment and to persistence with therapy. The IRB approved the study. RESULTS: Patients from the cohort were predominantly middle-aged (mean ± SD age: 38.5 ± 12.9 years) females (87.6%) with 5.4 ± 2.6 months of disease duration. The cohort contributed to 1538 patient-years of follow-up. Twelve patients received incidental OHSI at a follow-up of 85 ± 44.5 months. The OHSI incident global rate was 8/1000 patient-years. Longer symptom duration at cohort referral (OR: 1.313, 95%CI: 1.02-1.68, p = 0.032) and a higher number of flares/patient (OR: 1.608, 95%CI: 1.05-1.61, p = 0.015) predicted OHSI. OHSI patients had more severe flares than their counterparts, and the opposite figure was true for mild flares. CONCLUSION: Early referral for appropriate management and flare control may prevent OHSI in Hispanic recent-onset RA patients.

Assuntos

RESUMO

BACKGROUND: Recurrence occurs in up to 20% of patients with stage II colon cancer operated on for cure. Although postoperative intra-abdominal infection has been linked with an increased risk of recurrence, the association is controversial. The aim was to investigate the impact of postoperative intra-abdominal infection on disease-free survival and disease-specific survival in patients with stage II colon cancer. METHODS: Patients undergoing elective surgery for colon cancer stage II, between 2003 and 2014, were included. Patients with anastomotic leak or intra-abdominal abscess were included in the infection group. We used the Kaplan-Meier method to represent the distribution of survival and the Cox proportional hazards model to estimate the contribution of relevant clinicopathological factors with prognosis. RESULTS: Postoperative intra-abdominal infection was diagnosed in 37 of 363 (10.2%) patients. Perioperative blood transfusion was more frequent in patients with infection (p = 0.008). Overall 5-year disease-free survival rate was 85.1%. Disease-free survival at 5 years was lower in patients with postoperative intra-abdominal infection (52.8 vs 88.7%; p < 0.001), perineural invasion (p = 0.001), lymphovascular invasion (p = 0.001), pT4 (p = 0.013), and in patients with adjuvant chemotherapy (p = 0.013). Multivariate analysis showed that postoperative intra-abdominal infection (HR 4.275; p < 0.001), perineural invasion (HR 2.230; p = 0.007), and lymphovascular invasion (HR 2.052; p = 0.016) were all significant independent predictors of reduced disease-free survival. Regarding specific survival, independent significant prognostic factors were the number of lymph nodes, lymphovascular invasion, and postoperative intra-abdominal infection. CONCLUSION: In this series of patients with stage II colon cancer, postoperative intra-abdominal infection has an independent negative impact on disease-free survival and disease-specific survival.

Assuntos

RESUMO

Main group element coordination polymers (MGE-CPs) are important compounds for the development of multifunctional materials. However, there has been a shortage of studies regarding their structural, optical, catalytic, mechanical, and antibacterial properties. This work presents an exhaustive study of a set of crystalline MGE-CPs obtained from bismuth and indium metals and iminodiacetate, 1,2,4,5-benzenetetracarboxylate, and 2,2'-bipyridine as building blocks. An in-depth topological analysis of the networks was carried out. Additionally, nanoindentation studies were performed on two representative low-dimensional compounds in order to find the relationships between their structural features and their intrinsic mechanical properties (hardness and elasticity). The solid-state photoluminescence (SSPL) properties were also studied in terms of excitation, emission, lifetimes values, and CIE chromaticites. Moreover, the heterogeneous catalytic activities of the compounds were evaluated with the cyanosilylation reaction using a set of carbonylic substrates under solvent-free conditions. Finally, the inhibitory effect of the Bi-CPs on the growth of microorganisms such as Escherichia coli, Salmonella enterica serovar Typhimurium, and Pseudomonas aeruginosa, which are associated with relevant infectious diseases, is reported.

RESUMO

With the limitations of surgical reconstructive procedures, the growing number of gunshot wounds, burns, and work accidents in Mexico that result in complex facial deformities leaves only 1 option-face transplantation. The National Institute of Medical Sciences and Nutrition "Salvador Zubiran" (INCMNSZ) has performed transplants since 1971. We at INCMNSZ undertook the 1st bilateral upper-limb transplantation in Latin America in 2012. We are willing to continue in this manner toward conducting face transplantation at our institute. To this end, we identified and solved various challenges. The 1st challenge was acceptance and inclusion of vascularized composite allotransplantation (VCA) within general Mexican health law and approval of the face transplantation procedure. Subsequently, the health ministry provided a license to INCMNSZ to perform the procedure. The 2nd challenge concerned who would pay for the procedure. The costs will be paid by the patient (1st-party payer), social security institutions (2nd-party payers), and the health ministry (3rd-party payer). The 3rd challenge was to maintain ongoing surgical training of the team using cadavers. The fourth challenge was to locate donors; toward this end, we developed a campaign for promoting face donation in social media, making a comic book, and training organ and tissue coordinators to further VCA. Thus, INCMNSZ has the legal, administrative, medical, and surgical wherewithal to accomplish face transplantation.

Assuntos

RESUMO

Phase angle (PA) is the most established parameter from bioelectrical impedance analysis (BIA) for diagnosis of malnutrition and clinical prognosis, both associated with changes on cellular membrane integrity and alterations on fluids balance. PA expresses changes in quantity and quality of soft tissue mass (ie, cell membrane permeability and soft tissue hydration). A large body of clinical trials propose PA as a useful prognostic marker in clinical conditions like liver cirrhosis and breast, colon, pancreatic and lung cancer; positive association between PA and survival was also observed in surgical and HIV infected patients. Several authors suggest that PA can be an important tool in the evaluation of the clinical result or of the progression of the disease, and it can even be superior to other nutritional, biochemical or anthropometric indicators. Lack of reference values has limited its use in clinical and epidemiological situations. The purpose of this review is to describe PA reference values according to different clinical conditions as proposed in published scientific works.

El ángulo de fase (AF) es el parámetro de la bioimpedancia (BIA) mayormente establecido para el diagnóstico de la desnutrición y el pronóstico clínico, ambos asociados con cambios en la integridad de la membrana celular y las alteraciones en el balance de líquido. El AF expresa cambios en la cantidad y la calidad de la masa de los tejidos blandos (es decir, permeabilidad de la membrana celular e hidratación). Una gran cantidad de ensayos clínicos proponen el AF como un marcador pronóstico útil en condiciones clínicas, como en cirrosis hepática, en cáncer de mama, colon, páncreas, pulmón, también se observó en pacientes con VIHpositivos, y quirúrgicos una asociación positiva entre el AF y la supervivencia. Varios autores sugieren que el AF puede ser una herramienta importante para evaluar el resultado clínico o para evaluar la progresión de la enfermedad y este puede ser superior a otros indicadores nutricionales, bioquímicos o antropométricos. La falta de valores de referencia ha limitado su uso en situaciones clínicas y epidemiológicas. El objetivo de esta revisión es describir los valores de referencia de ángulo de fase según diferentes condiciones clínicas propuestos en los trabajos científicos publicados.

Assuntos

RESUMO

INTRODUCTION: Despite the fact that the frequency of hypolactasia and lactose intolerance is similar in both chronic idiopathic ulcerative colitis patients and the general population, the elimination of dairy products from the patient's diet is a habitual recommendation. Hypolactasia is common in Mexico, but its relation to chronic idiopathic ulcerative colitis has not been established. AIMS: To evaluate lactose digestion and lactose intolerance in persons with chronic idiopathic ulcerative colitis. MATERIAL AND METHODS: Thirty-nine patients with confirmed chronic idiopathic ulcerative colitis diagnosis were included in the study (mean: 31 years, range: 15 to 38). Twenty-two patients presented with rectosigmoid involvement and the remaining patients with pancolitis. No patient showed inflammatory activity according to the Truelove-Witts criteria and all consumed dairy products before diagnosis. A prospective, controlled, double-blind, cross-over study was designed. Patients randomly received 12.5 g of lactose or maltose in 250 cc water- each test 72 hours apart - and ydrogen was measured in exhaled air before disaccharide ingestion and then every 30 minutes for 3 hours. Digestion was considered deficient when there was an increase in hydrogen of at least 20 ppm. Symptom intensities were evaluated by Visual Analog Scales before, during, and after the hydrogen test. Differences between the groups were contrasted with the Mann-Whitney U and the Wilcoxon tests. RESULTS: Eighteen patients (46%) presented with deficient lactose digestion. No significant differences were found in the symptoms, extension, or progression of chronic idiopathic ulcerative colitis between patients that could digest and those that could not digest lactose. No patient had symptom exacerbation with the disaccharides used. CONCLUSIONS: Lactose digestion deficiency frequency is similar in subjects with chronic idiopathic ulcerative colitis and in healthy individuals in Mexico. We do not know whether higher doses could have some effect, but symptoms in patients with inactive chronic idiopathic ulcerative colitis were not modified using 12.5 g of lactose/day.

Assuntos

RESUMO

Introducción: la capacidad de los microorganismos para formar y mantenerse en el biofilm tiene un alto impacto en las infecciones crónicas, pues el mismo protege y nutre a comunidades de microorganismos que pueden influir en el tratamiento ortodóncico, aumentando la incidencia de caries y enfermedad periodontal. Objetivo: comparar la condición periodontal inicial y la alcanzada luego de la terapia básica periodontal de los pacientes que concurren a la consulta para iniciar un tratamiento ortodóncico. Materiales y métodos: se evaluaron 10 pacientes entre 14 y 30 años que concurrieron al servicio de la Cátedra de Ortodoncia de la Facultad de Odontología de la UBA. Se tomaron el índice de placa de Silness y Loe y gingival de Loe y Silness, la profundidad de sondaje y hemorragia gingival al sondaje en todas las piezas dentarias presentes en la boca del paciente. De la zona de los primeros premolares superiores se eliminó la placa supragingival y se tomaron muestras subgingivales, las cuales fueron colocadas en solución fisiológica y medio de transporte VMGAIII. Simultáneamente se realizaron extendidos del materiales y se coloreó con la técnica de Gram y de Giemsa. Luego de que los pacientes recibieran enseñanza de técnicas de higiene bucal y/o terapia básica periodontal, se volvieron a registrar dichos índices. Resultados: el índice de placa inicial presentó una mediana de 2.5 y post terapia básica, una mediana de 1.0 con una diferencia estadísticamente significativa (p=0.005) según Wilcoxon Signed Rank Test. El indice gingival inicial presentó una mediana de 2.0 y post terapia básica fue de 1.0 con una diferencia estadísticamente significativa (p<0.005 Wilcoxon Signed Test). La profundidad de sondaje pretratamiento presentó una mediana de 3.0mm con sangrado al sondaje positivo y microbiota compatible con gingivitis y periodontitis leve. Luego de enseñanza de técnicas de higiene bucal la misma fue de 1 mm con sangrado al sondaje negativo.

Assuntos

RESUMO

For patients with severe hand deformities due to rheumatoid arthritis, we propose an allotransplantation of an osteomyotendinose structure (OMTS), preserving the recipient's skin and sensory nerves. Our objective was to develop the surgical technique in a 10 cadavers, five as donors and five as recipients. The donor's hand was 10% to 15% smaller than the recipient's. Dissections were performed by two surgical teams under magnification. In the donor, the OMTS was procured at the distal third of the forearm, maintaining the integrity of the arterial system, with its concomitant veins and motor branches of the median and ulnar nerves, leaving the skin envelope. In the recipient, the OMTS was removed, taking care to preserve the cutaneous cover with the digital arteries in continuity with the superficial palmar arch and radial and ulnar arteries. Also, the digital nerves were maintained in the skin flap, in continuity with the median and ulnar nerves. Their motor branches were divided after emergence from the main nerves. The superficial dorsal veins and radial nerve were kept adhered to the cutaneous cover. Then, the donor OMTS was placed within the recipient cutaneous flap; all the anatomic structures were repaired. The average surgical time was 780 minutes. Methylene blue was present in the digital arteries. There were no difficulties in the anatomic repair. The surgical technique is quite laborious, especially the dissection of the recipient interdigital spaces. Due to the requirement for arterial system integrity, the cutaneous flap must be viable. Also, the allotransplanted OMTS has all necessary conditions to obtain good tissue perfusion for subsequent function. Procurement without skin permits a greater opportunity to find donors, and greater social and personal acceptance by the recipient.

Assuntos

RESUMO

Rice hulls and sawdust are two common C-rich wastes derived from rice and timber agro-industries in subtropical NE Argentina. An alternative to the current management of these wastes (from bedding to uncontrolled burning) is composting. However, given their C-rich nature and high C/N ratio, adequate composting requires mixing with a N-rich waste, such as poultry manure. The effect of different proportions of poultry manure, rice hulls and/or sawdust on composting efficiency and final compost quality was studied. Five piles were prepared with a 2:1 and 1:1 ratio of sawdust or rice hulls to poultry manure, and 1:1:1 of all three materials (V/V). Different indicators of compost stability and quality were measured. Thermophilic phase was shorter for piles with rice hulls than for piles with sawdust (60 days vs. 105 days). Time required for stability was similar for both C-rich wastes (about 180 days). Characteristics of final composts were: pH 5.8-7.2, electrical conductivity 2.5-3.3 mS/cm, organic C 20-26%, total N 2.2-2.9%, lignin 19-22%, total Ca 18-24 g/kg, and extractable P 6-8 g/kg, the latter representing 60% of total P. Nitrogen conservation was high in all piles, especially in the one containing both C-rich wastes. Piles with sawdust were characterized by high total and available N, while piles with only rice hulls had higher Si, K and pH. Extractable P was higher in 1:1 piles, and organic C in 2:1 piles.

Assuntos

RESUMO

Background: There is substantial evidence about comorbidity between eating disorders and obsessive-compulsive disorder. Aim: To compare the presence of obsessions and/or compulsions and its prevalence in eating disordered patients and normal subjects. Subjects and methods: A structured clinical interview, the Eating Altitudes Test (EAT-40), the Eating Disorder Inventory (EDI), and the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) were administered to 54 patients who fulfilled the DSM-IV diagnostic criteria for eating disorders and to 54 normal female students. Results: Patients with eating disorders scored significantly higher on the EAT-40 and on the EDI and its factors, induding the comorbidity scale (p<0.001). Prevalence of obsessive-compulsive symptoms within a moderate and/or severe degree was significantly higher in eating disordered patients (26 percent versus 1. 9 percent) (p<0.001), particularly among anorexic patients (33.3 percent) (p= 0.009). Y-BOCS and its subscale Y-BCS emerge as the best indicators of eating disorders and correlated significantly with the EDI items drive for thinness, interoceptive awareness, bulimia, body dissatisfaction and ineffectiveness (p< 0.05). Conclusions: Obssesive-compulsive symptoms and their prevalence rates are confirmed according to the evidence in eating disordered patients, mainly among patients suffering from anorexia nervosa that scored higher within moderate and severe degrees.

Antecedentes: Existe considerable evidencia sobre la comorbilidad entre trastornos de la conducta alimentaria y trastorno obsesivo-compulsivo. Objetivo: Comparar la presencia de trastorno obsesivo-compulsivo y sus prevalencia, en mujeres con y sin patologías alimentarias. Sujetos y Métodos: Se administró una entrevista clínica estructurada, el Test de Actitudes Alimentarias (EAT-40), el Inventario de Desórdenes Alimentarios (EDI), la Escala de Obsesividad-Compulsividad de Yale-Brown (Y-BOCS) a 54 pacientes que satisficieron los criterios diagnósticos según el DSM-IV para trastornos de la conducta alimentaria y a 54 estudiantes sin problemas alimentarios. Resultados: Los puntajes de todos los indicadores, incluidas las escalas de comorbilidad, fueron más altos en las pacientes (p < 0,001). La prevalencia síntomas obsesivo-compulsivos de grado moderado y/o superior fue significativamente mayor en las pacientes alimentarias (26 por ciento versus 1, 9 por ciento) (p < 0,001), particularmente en las pacientes anorécticas (33,3 por ciento) (p = 0,009). La escala Y-BOCS y la subescala Y-BCS emergen como los mejores indicadores para discriminar patologías alimentarias y se correlacionaron significativamente con los ítems motivación por la delgadez, consciencia interoceptiva, bulimia, insatisfacción corporal y sentimientos de ineficacia personal (p < 0,05). Conclusiones: Se confirma la presencia y la prevalencia de trastorno obsesivo-compulsivo en los desórdenes alimentarios según la evidencia, principalmente en las pacientes portadoras de anorexia nerviosa que obtuvieron puntajes más altos en los niveles moderado y severo.

Assuntos

RESUMO

Se presenta una estrategia metodológica con el propósito de desarrollar la capacidad de respuesta de los estudiantes ante los cambios constantes del entorno en que se desempeñan en las carreras de medicina, estomatología y enfermería.

A methodological strategy is presented with the objective of developing the capacity of the students to give an answer to the constant changes occurring in the setting in which they carry out their activities in the medical, dental and nursing careers.

Assuntos

RESUMO

Background: There is substantial evidence about comorbidity between eating disorders, depression and social anxiety. Aim: To compare the presence of social anxiety and depression and its prevalence in eating disordered patients and normal subjects. Subjects and Methods: A structured clinical interview, the Eating Attitudes Test (EAT-40), the Eating Disorder Inventory (EDI), the Liebowitz Social Anxiety Scale (LSAS) and the Hamilton Depression Rating Scale (HDRS) were administered to 54 patients that fulfilled the DSM-IV diagnostic criteria for eating disorders and to 54 normal female students. Results: Patients with eating disorders ranked significantly higher on the EAT-40 and on the EDI and its factors, including comorbidity scales (p < 0.001). Prevalence of social anxiety was significantly higher in eating disordered patients (40. 7 percent versus 3.7 percent) (p < 0.001), particularly among anorexics (66.6 percent) (p < 0,001) and 14.8 percent of the eating disordered patients ranked within the severe range (p < 0.001). Theriskfor moderate to very severe social anxiety was 5.5 times higher among them. Prevalence of depressive symptoms was significantly higher in eating disordered patients (7& 5 percent versus 0 percent) (p < 0.001), specially among anorexics (66.6 percent) (p < 0,001) and 18.5 percent of the eating disordered patients ranked within the severe or very severe range (p < 0.001). The risk for moderate to very severe depression was 23.7 times higher among them. Conclusions: Depressive and/or social anxiety disorders and their prevalence rates are confirmed according to the evidence in eating disordered patients, mainly among patients suffering from anorexia nervosa.

Antecedentes: Existe considerable evidencia sobre la comorbilidad entre trastornos de la conducta alimentaria, depresión y fobia social. Objetivo: Comparar la presencia de ansiedad social y depresión y sus prevalencias, en mujeres con y sin patologías alimentarias. Sujetos y Métodos: Se administró una entrevista clínica estructurada, el Test de Actitudes Alimentarias (EAT-40), el Inventario de Desórdenes Alimentarios (EDI), la Escala de Ansiedad Social de Liebowitz (LSAS) y la Escala de Hamilton para Depresión (HDRS) a 54 pacientes que satisficieron los criterios diagnósticos según el DSM-IVpara trastornos de la conducta alimentaria y a 54 estudiantes sin problemas alimentarios. Resultados: Los puntajes de todos los indicadores, incluidas las escalas de comorbilidad, fueron mßs altos en las pacientes (p < 0,001). La prevalencia de ansiedad social fue significativamente mayor en las pacientes alimentarias (40,7 por ciento versus 3,7 por ciento) (p < 0,001), particularmente en las anorécticas (66,6 por ciento) (p < 0,001) y el 14,8 por ciento de las pacientes con patologías alimentarias obtuvo puntajes en los rangos severo o muy severo (p < 0,001). El riesgo de presentar ansiedad social moderada a muy severa en las pacientes alimentarias fue 5,5 veces mayor. La prevalencia de síntomas depresivos fue significativamente mayor en las pacientes alimentarias (18,5 por ciento versus 0 por ciento) (p < 0,001), especialmente en las anorécticas (66,6 por ciento) (p < 0,001) y 18,5 por ciento de las pacientes con trastornos alimentarios obtuvo puntajes en el rango severo (p < 0,001). El riesgo de presentar depresión moderada a muy severa en las pacientes alimentarias fue 23,7 veces mayor. Conclusiones: Se confirma la presencia y la prevalencia de trastornos depresivos y de ansiedad social en los desórdenes alimentarios según la evidencia, principalmente en las pacientes portadoras de anorexia nerviosa.

Assuntos

RESUMO

Galectin-1 (Gal-1) is a widely expressed beta-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, we identified cytoplasmic Gal-1 expression in these tumor cells by cytofluorometry. DNA fragmentation, caspase-3, -8, and -9 activation, loss of mitochondrial membrane potential (DeltaPsim), cytochrome c (Cyt c) release, and FasL expression suggested that relatively high concentrations of exogenously added recombinant Gal-1 (rGal-1) induced apoptosis by the mitochondrial and death receptor pathways. These pathways were independently activated, as the presence of the inhibitor of caspase-8 or -9 only partially prevented Gal-1-effect. On the contrary, low concentrations of Gal-1 significantly promoted cell proliferation, without inducing cell death. Importantly, the presence of the disaccharide lactose prevented Gal-1 effects, suggesting the involvement of the carbohydrate recognition domain (CRD). This study provides strong evidence that Gal-1 is a novel biphasic regulator of Leydig tumor cell number, suggesting a novel role for Gal-1 in the reproductive physiopathology.

Assuntos