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BACKGROUND: Food-grade titanium dioxide (E171), a white colourant widely used in ultra-processed food products, has been banned in the European Union. However, its usage is still permitted in medicines, and in several other countries. The estimated intake of E171 in children is higher than in adults, which led us to hypothesise that E171 induces differential effects depending on age, with adult mice being the most susceptible due to age, despite the lower dose. AIM: To evaluate the effects of oral administration of E171 on intestinal permeability, ileum, and colon histology, and how these effects impact anxious and depressive behaviour in young and adult mice of both sexes. METHODS: Young and adult mice of both sexes C57BL/6 mice received 10 mg/kgbw E171/3 times per week for 3 months. E171 was administered orally in water by pipetting, while control groups only received drinking water, then intestinal permeability, histology and animal behaviour were analysed. RESULTS: E171 showed an amorphous shape, primary particles sized below 1 µm and anatase crystalline structure. Oral administration of E171 disrupted the intestinal permeability in adult male and female mice, but no effects were observed in young mice of both sexes. E171 promoted ileal adenoma formation in half of the adult female population, moreover hyperplastic crypts, and hyperplastic goblet cells at histological level in adult mice of both sexes. The colon presented hyperplastic goblet cells, hyperchromatic nuclei, increased proliferation and DNA damage in adult mice of both sexes. The anxiety and depressive behaviour were only altered in adult mice treated with E171, but no changes were detected in young animals of both sexes. CONCLUSIONS: Adult mice displayed higher susceptibility in all parameters analysed in this study compared to young mice of both sexes.

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Resumen ANTECEDENTES: La ruptura hepática es una complicación excepcional del síndrome de HELLP. La incidencia mundial de enfermedades hipertensivas relacionadas con el embarazo es del 4.5%, con ruptura hepática en aproximadamente 1 de cada 250,000 embarazos. La mortalidad estimada es del 90%. CASO CLÍNICO: Paciente multigesta, de 34 años, que ingresó a la unidad hospitalaria a las 34.2 semanas del embarazo debido a una encefalopatía hipertensiva y dolor súbito en el epigastrio e hipocondrio. En la exploración física se encontró hemodinámicamente estable; los estudios de laboratorio informaron: trombocitopenia, función hepática alterada, enzimas hepáticas elevadas y hemólisis. La ecografía hepática reportó hematoma subcapsular. Se realizó laparotomía de urgencia, con cesárea y hemoperitoneo masivo. Se observó un hematoma hepático subcapsular, con ruptura y hemorragia activa. El tratamiento consistió en taponamiento hepático; sin embargo, persistió el sangrando, por lo que se practicó una laparotomía de segunda instancia, con hemorragia activa. Se hizo el empaquetamiento y a las 48 horas se retiró. La recuperación fue favorable y se dio de alta a los 16 del ingreso, sin complicaciones adicionales. CONCLUSIONES: El caso aquí expuesto sugiere que los estados hipertensivos del embarazo, principalmente la preeclampsia, eclampsia y síndrome de HELLP, son el principal factor de riego de formación de un hematoma hepático, y en casos excepcionales la subsiguiente ruptura, incluso después del parto.

Abstract BACKGROUND: Hepatic rupture is an extremely rare complication in HELLP syndrome. The worldwide incidence of pregnancy-related hypertensive diseases is 4.5%, with liver rupture occurring in approximately 1 in 250,000 pregnancies. Mortality is high when it is present, which can be up to 90%. CLINICAL CASE: A 34-year-old multigravida woman was admitted at 34.2 weeks of gestation to the emergency room due to clinical hypertensive encephalopathy and sudden onset of pain in epigastrium and hypochondrium. At admission hemodynamically stable, blood work only reported thrombocytopenia, hepatic function altered with elevated liver enzymes and hemolysis. The hepatic ultrasound reported hematoma subcapsular. An emergency laparotomy with c- section was performed with a massive hemoperitoneum was observed. A subcapsular hepatic hematoma with rupture and active hemorrhage managed with hepatic packing. However, the patient continued to bleed, a second-look laparotomy was performed with active hemorrhage, which led to packing and 48 hours the removal of the packing. Her recovery was favorable and was discharged 16 days after her admission, with no subsequent complications. CONCLUSIONS: The presentation of the case and bibliographic review suggests that hypertensive states of pregnancy, mainly preeclampsia, eclampsia and HELLP syndrome, are the main risk factor for the formation of a hepatic hematoma, and in exceptional cases the consequent rupture, even after delivery. Birth.

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Resumen ANTECEDENTES: La corea gravídica es un padecimiento poco común, caracterizado por movimientos espontáneos irregulares, no predecibles, de duración breve, que cambian de localización sin secuencia definida, y que poseen la particularidad de ser involuntarios. En la actualidad, su incidencia se estima en 1 caso por cada 2275 embarazos. CASO CLÍNICO: Paciente de 30 años, 45 kg, talla 1.65 m, IMC 16.5, tensión arterial 110-78 mmHg, frecuencia cardiaca de 136 lpm, frecuencia respiratoria de 19 rpm, saturación de oxígeno 98%. Inició con movimientos coreicos a las 12 semanas de embarazo y titulación de anticuerpos antinucleares 1:320, anticuerpos anti-ADN de 150 UI/mL, C3 en 126 mg/dL y C4 en 31 mg/dL. Se estableció el diagnóstico de lupus eritematoso sistémico con base en los criterios de EULAR/ACR. A las 32 semanas tuvo amenaza de parto pretérmino y progresión, a pesar de la útero-inhibición, preeclampsia con criterios de severidad y manifestaciones neuropsiquiátricas con exacerbación de los movimientos coreicos. La resonancia magnética no reportó hallazgos patológicos y se descartó el síndrome de anticuerpos antifosfolipídicos secundario. Luego de la finalización del embarazo remitieron los movimientos involuntarios característicos de la corea gravídica. CONCLUSIÓN: El control prenatal en el primer trimestre es fundamental para un de-senlace favorable de las pacientes; el inicio temprano de la inmunoterapia es decisivo en los desenlaces perinatales pues disminuye la morbilidad y mortalidad asociadas. La corea es una manifestación clínica incapacitante, de ahí la importancia de identificar su causa e iniciar el tratamiento oportuno.

Abstract BACKGROUND: Chorea gravidarum is a rare condition characterised by irregular, unpredictable, spontaneous movements of short duration, changing location without defined sequence, and with the peculiarity of being involuntary. Currently, its incidence is estimated at 1 case per 2275 pregnancies. CLINICAL CASE: Patient aged 30 years, 45 kg, height 1.65 m, BMI 16.5, blood pressure 110-78 mmHg, heart rate 136 bpm, respiratory rate 19 rpm, oxygen saturation 98%. She started with choreic movements at 12 weeks of pregnancy and titration of antinuclear antibodies 1:320, anti-DNA antibodies 150 IU/mL, C3 at 126 mg/dL and C4 at 31 mg/dL. The diagnosis of systemic lupus erythematosus was established based on EULAR/ACR criteria. At 32 weeks she had threatened preterm labour and progression, despite utero-inhibition, preeclampsia with severity criteria and neuropsychiatric manifestations with exacerbation of choreic movements. MRI reported no pathological findings and secondary antiphospholipid antibody syndrome was ruled out. After termination of pregnancy the involuntary movements characteristic of chorea gravidarum subsided. CONCLUSION: Prenatal monitoring in the first trimester is essential for a favourable patient outcome; early initiation of immunotherapy is crucial for perinatal outcomes as it decreases associated morbidity and mortality. Chorea is a disabling clinical manifestation, hence the importance of identifying its cause and initiating timely treatment.

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Titanium dioxide food grade (E171) is one of the most used food additives containing nanoparticles. Recently, the European Food Safety Authority indicated that E171 could no longer be considered safe as a food additive due to the possibility of it being genotoxic and there is evidence that E171 administration exacerbates colon tumor formation in murine models. However, less is known about the effects of E171 accumulation once the exposure stopped, then we hypothesized that toxic effects could be detected even after E171 removal. Therefore, we investigated the effects of E171 exposure after being removed from colon cell cultures. Human colon cancer cell line (HCT116) was exposed to 0, 1, 10 and 50 µg/cm2 of E171. Our results showed that in the absence of cytotoxicity, E171 was accumulated in the cells after 24 of exposure, increasing granularity and reactive oxygen species, inducing alterations in the molecular pattern of nucleic acids and lipids, and causing nuclei enlargement, DNA damage and tubulin depolymerization. After the removal of E171, colon cells were cultured for 48 h more hours to analyze the ability to restore the previously detected alterations. As we hypothesized, the removal of E171 was unable to revert the alterations found after 24 h of exposure in colon cells. In conclusion, exposure to E171 causes alterations that cannot be reverted after 48 h if E171 is removed from colon cells.

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Food-grade titanium dioxide (E171) is widely used as a food additive, and it is known that after oral consumption, E171 is translocated into the bloodstream reaching the highest titanium level at 6 h. E171 is accumulated in some organs triggering toxicity, but the effects on the blood parameters after oral consumption have been less studied. Recently, evidence shows that oral exposure to E171 induces behavioral signs of anxiety and depression. The relation between blood alterations and psychiatric disorders has been previously demonstrated. However, the oral exposure to E171 effects on alterations in blood parameters and effects linked to alterations in animal behavior has not been explored. In this short communication, we aimed to investigate the effects of E171 on specific blood parameters (hematocrit, hemoglobin, number of erythrocytes, and leukocytes) and anxiety and compulsive-like behavior in males and females orally exposed to ~5 mg/kg for 4 weeks. The results showed that E171 decreased hematocrit and hemoglobin in male but not in female mice while leukocyte and erythrocyte count remained unaltered. Oral consumption of E171 decreased the levels of anxiety-like behavior in females but not in male mice, while compulsive-like behavior was increased in both male and female mice.

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The Organisation for Economic Co-operation and Development has listed thirteen engineered nanomaterials (ENM) in order to investigate their toxicity on human health. Silicon dioxide (SiO2) and titanium dioxide (TiO2) are included on that list and we added indium tin oxide (ITO) nanoparticles (NPs) to our study, which is not listed on OECD suggested ENM to be investigated, however ITO NPs has a high potential of industrial production. We evaluate the physicochemical properties of SiO2 NPs (10-20 nm), TiO2 nanofibers (NFs; 3 µm length) and ITO NPs (<50 nm) and the impact of protein-corona formation on cell internalization. Then, we evaluated the toxicity of uncoated ENM on human lung epithelial cells exposed to 10 and 50 µg/cm2 for 24 h. TiO2 NFs showed the highest capability to adsorb proteins onto the particle surface followed by SiO2 NPs and ITO NPs after acellular incubation with fetal bovine serum. The protein adsorption had no impact on Alizarin Red S conjugation, intrinsic properties for reactive oxygen (ROS) formation or cell uptake for all types of ENM. Moreover, TiO2 NFs induced highest cell alterations in human lung epithelial cells exposed to 10 and 50 µg/cm2 while ITO NPs induced moderated cytotoxicity and SiO2 NPs caused even lower cytotoxicity under the same conditions. DNA, proteins and lipids were mainly affected by TiO2 NFs followed by SiO2 NPs with toxic effects in protein and lipids while limited variations were detected after exposure to ITO NPs on spectra analyzed by Fourier Transform Infrared Spectroscopy.

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Diversos elementos proporcionan la información necesaria para el consumo de alimentos, entre ellos: el sabor, la textura y temperatura. Sin embargo, se requiere de su vinculación con la experiencia y las características ambientales para integrar el comportamiento alimentario. La evidencia experimental sugiere que el sexo y la interacción social son factores importantes para el consumo de alimentos. Esta investigación evaluó los efectos del género y de la interacción social sobre el consumo de alimentos. Adicionalmente, para tal objetivo se propone el uso del Catálogo Conductual de Interacción Alimentaria (CCIA). Participaron veinticuatro individuos de ambos sexos, entre 20 y 28 años, desconocidos entre ellos, fueron expuestos a diversos alimentos usuales y novedosos evaluando su interacción alimentaria. Los participantes fueron divididos en tres grupos. El grupo 1 fue integrado por hombres, el grupo 2 por mujeres y el grupo 3 por ambos géneros. Los resultados mostraron que el grupo 1 y 2 consumieron cantidades similares de alimento en comparación con el grupo 3 que presentó un escaso consumo. Se registró una fuerte influencia de las mujeres sobre los hombres para promover su consumo de alimento, fenómeno que fue denominado como "Efecto Eva". Concluimos que el género es un importante factor en el estudio del fenómeno alimentario.

Several elements provide information for food consumption, among them: flavor, texture and temperature. These elements related to environmental characteristics and experience integrate the feeding behavior. Experimental evidence suggests that sex and social interaction are important factors for food consumption. This study evaluated effects of sex and social interaction on food consumption. Additionally, our proposal is to use Behavioral Catalogue of Feeding Interaction (BCFI). Twenty-four human subjects, both sexes, between 20 and 28 years-old, which were not known between them, were exposed to different familiar and novel foods, evaluating the feeding interaction. Subjects were divided in three groups. First group was formed by man, second by woman; and third group by subjects of both sexes. Data suggest that group 1 and 2 registered similar food consumption in comparison with the group 3 that registered a little consumption. A strong influence of women was registered on men to stimulate its food consumption, phenomenon that was denominated "Eva Effect". We conclude that sex is an important factor in the study of feeding phenomenon.

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Este estudio evaluó los efectos de las instrucciones no explícitas sobre la conducta alimentaria. Participaron cinco jóvenes entre los 19 y 24 años, a quienes se les observó durante dos fases experimentales a dos tipos de alimento nutricional (fruta y agua) y no nutricional (papas fritas y refresco). Los participantes fueron f lmados en dos programas de video. En la primera fase se grabaron videos musicales y en la segunda, un documental sobre hábitos alimentarios saludables. Los resultados mostraron que las instrucciones no explícitas modificaron la conducta alimentaria. Sin embargo, es necesario evaluar si estas modificaciones persisten a largo plazo.

This study evaluated the effects of non-explicit audiovisual instructions on feeding behaviour. Five adults between 19 and 24 years-old participated as subjects, which were exposed at nutritional (fruit and water) or no nutritional (chips and soda) foods during two experimental phases. Subjects were videotaped and exposed to two video programs in different phases. In the first phase, subjects were exposed to a musical video, in the second phase subjects were exposed to a program about health eating habits. The results showed that non-explicit audiovisual instructions modified feeding behaviour. However, will be necessary evaluate if these modification could persist for longer time.

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El objetivo del presente estudio fue evaluar los efectos del consumo de café con y sin cafeína sobre la conducta alimentaria en ratas. Dieciséis ratas fueron expuestas a 2, 3, 4 y 5 gramos de café con cafeína y sin cafeína diluidas en 200 ml de agua. Los sujetos fueron asignados a cuatro grupos conformados por dos hembras y dos machos cada uno. Los grupos 1 y 2 fueron expuestos a café con cafeína y los grupos 3 y 4 a café sin cafeína. La exposición de café para los grupos 1 y 3 fue de forma ascendente (2 g, 3 g, 4 g y 5 g) y para los grupos 2 y 4 de forma descendente (5 g, 4 g, 3 g y 2 g). El experimento fue dividido en nueve fases. Las fases 1, 3, 5, 7 y 9 tuvieron una duración de diez días, en cada una se proporcionaron 200 ml de agua y 50 g de alimento. Las fases 2, 4, 6 y 8 tuvieron una duración de cinco días cada una, en las cuales se les proporcionó las concentraciones de café y 50 g de alimento. Los resultados mostraron que las concentraciones bajas de café registraron un mayor consumo en comparación con las concentraciones altas en ambas condiciones (ascendente y descendente). Por otra parte, el café sin cafeína fue más consumido que el café con cafeína.

The objective of the present study was to evaluate the effects of coffee intake with and without caffeine on feeding behavior in rats. Sixteen rats was exposed to 2g, 3g, 4g and 5g of coffee with caffeine and without caffeine diluted in 200ml of water. Subjects were assigned to 4 groups conformed by 2 females and 2 males each one. Groups 1 and 2 were exposed to coffee with caffeine and groups 3 and 4 to coffee without caffeine. Exposed to coffee for groups 1 and 3 was of ascending form (2 g, 3 g, 4 g, and 5 g) and for groups 2 and 4 of descendent form (5 g, 4 g, 3 g and 2 g). The experiment was divided in 9 phases. Phases 1, 3, 5, 7 and 9 lasted of 10 days, in each one were provided 200ml of water and 50g food. Phases 2, 4, 6 and 8 lasted of 5 days; in each one provided the concentrations of coffee and 50g food. The results showed that the low concentrations of coffee registered a greater consumption in comparison with the high concentrations in both conditions (ascending and descendent). On the other hand, coffee without caffeine was more consumed than coffee with caffeine.

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El objetivo de este estudio fue analizar el efecto de la información nutricional sobre el consumo de frutas y verduras. Participaron 25 niños entre los 3 y 5 años, expuestos a alimentos con alto y bajo valor nutricional. Los niños fueron divididos en 2 grupos (piloto y experimental) y expuestos a 4 fases. El grupo experimental recibió información sobre hábitos alimenticios, valor nutricional de los alimentos y beneficios del consumo de frutas y verduras. Los resultados mostraron que la información nutricional modificó la conducta alimentaría, ya que el consumo de alimentos poco nutritivos disminuyó. Sin embargo, el consumo de frutas y verduras no se incrementó. Los datos sugieren que la información nutricional influye en la adquisición de conductas alimentarías en niños preescolares, siempre y cuando incluya una duración y frecuencia adecuadas y sea reforzada en casa a través del modelamiento de los familiares.

This study evaluated effects of nutritional information about fruits and vegetables consumption. Participants were 25 children between 3 and 5 years-old, which were exposed at high and low nutritional foods. Subjects were divided in two groups (experimental and control) and exposed to four phases. Experimental group received nutritional information about dietetic habits, nutritional facts and benefits of fruits and vegetables consumption. Results showed that nutritional information modified the eating behaviour. Non-nutritious food consumption diminished, but fruits and vegetables consumption not increased. Data suggest that nutritional information modifies eating behaviour development in preschoolers, but is necessary a suitable duration and frequency with adequate reinforced in house through model of parents.

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Se realiza una breve reseña histórica sobre las aportaciones teóricas y experimentales de diversos investigadores que contribuyeron al desarrollo de la psicología experimental. En la primera parte, se presenta una revisión de los inicios de la psicofísica y la psicología experimental. Posteriormente se caracterizan las aportaciones más importantes que involucraron los estudios en psicología animal, estructural y funcional, finalizando con la propuesta conductista de Watson y Skinner como método para estudiar los fenómenos psicológicos. Finalmente, se discute la utilidad de los estudios de laboratorio desde la perspectiva del análisis del comportamiento, enfatizando los alcances y desarrollo en Estados Unidos y diversos países de Latinoamérica.

A brief history about theoretical and experimental contributions is described of researches to the development of the experimental psychology. In the first part, a revision of the beginnings of the psychophysic and experimental psychology appears. Later, the most important contributions are characterized those studies that involved the studies in psychology animal, structural and functional, finalizing with the Watson and Skinner's behaviorist proposal as the method to study the psychological phenomena. Finally, we discussed the utility of the laboratory studies from the perspective of experimental analysis of behavior, emphasizing the reaches and development in the United States and diverse countries of Latin America.

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We compared the frequency of Pneumocystis in 126 sudden infant death syndrome (SIDS) cases with a control group of 24 infants from the San Diego SIDS/SUDC Research Project who died of accidental or inflicted injuries. Cysts were identified in 33% of SIDS cases and 29% of controls. We conclude that Pneumocystis is not a direct cause of SIDS.

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Esta publicação apresenta os debates desenvolvidos durante o seminário - Sexualidades e Sociedades Contemporâneas, organizado pelo Programa de Estudos de Gênero e Sociedade da Universidade Academia de Humanismo Cristiano

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