RESUMO

An indirect MicroELISA system for the detection of anti-Aspergillus fumigatus antibodies using human anti-IgG conjugated to peroxidase was standardized. Serum samples of two patients having an aspergilloma diagnosed by clinical, microbiological, and serological criteria were used as positive controls. In addition, 119 serum samples of blood donors and 216 serum samples of patients having chronic pneumopathies were also studied. The standardized system may be used as a diagnostic complement for it is able to discriminate between antibody levels (IgG) in different clinical forms of aspergillosis and in those individuals having respiratory diseases which may favour the colonization by this pathogen.

Assuntos

Anticorpos Antifúngicos/isolamento & purificação , Aspergillus fumigatus/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Antígenos de Fungos/imunologia , Aspergilose/diagnóstico , Aspergilose/imunologia , Humanos