Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Int J Biol Macromol ; 186: 501-509, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34271043

RESUMO

Two homogeneous polysaccharides, GEP-3 and GEP-4, were purified from Gastrodia elata, a precious traditional Chinese medicine. Their structural characteristics were obtained using HPGPC, PMP-HPLC, LC/MS, FT-IR, NMR, and SEM methods. GEP-3 was 1,4-glucan with molecular weight of 20 kDa. Interestingly, GEP-4 comprised of a backbone of →[4)-α-Glcp-(1]10→[4)-α-Glcp-(1→]5[6)-ß-Glcp-(1]11→6)-α-Glcp-(3→ and two branches of ß-Glcp and p-hydroxybenzyl alcohol citrate, with repeating p-hydroxybenzyl alcohol attached to the backbone chain at O-6 position of →4,6)-α-Glcp-(1→ and O-1 position of →3,6)-α-Glcp-(1→. GEP-4 is a novel polysaccharide obtained and characterized for the first time. Bioactivity test indicated that both of them significantly promote the growth of Akkermansia muciniphila (Akk. muciniphila). Furthermore, GEP-3 and GEP-4 promoted the growth of Akk. muciniphila from high-fat diet (HFD) fecal microbiota. These results indicated that GEP-3 and GEP-4 were potential Akk. muciniphila growth promoters.


Assuntos
Gastrodia , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Akkermansia/efeitos dos fármacos , Akkermansia/crescimento & desenvolvimento , Akkermansia/isolamento & purificação , Animais , Dieta Hiperlipídica , Modelos Animais de Doenças , Fezes/microbiologia , Gastrodia/química , Microbioma Gastrointestinal , Camundongos , Estrutura Molecular , Hepatopatia Gordurosa não Alcoólica/microbiologia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Polissacarídeos/química , Polissacarídeos/isolamento & purificação
2.
Steroids ; 164: 108749, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33065151

RESUMO

A series of cinobufagin-3-yl nitrogen-containing-carbamate derivatives were designed, synthesized, and evaluated for their proliferation inhibition activities. The structure-activity relationships suggested that the substituents at C-16 was a crucial factor for the potency and that follows this trends: acetic ester â‰« benzoic ester ≈ hydroxy > carbamate. Compounds 3f, 3g, 3h, and 3i exhibited significant in vitro antiproliferative activities against the eight tested tumor cell lines, with IC50 values ranging from 8.1 to 237.4 nM. Furthermore, 3g tartrate (3g-TA) significantly inhibited tumor growth by 64.5%, 83.9%, and 93.0% at a doses of 4, 6, 8 mg/kg/qod by ip, respectively.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Bufanolídeos/farmacologia , Carbamatos/química , Desenho de Fármacos , Animais , Antineoplásicos/síntese química , Bufanolídeos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Estrutura Molecular , Solubilidade , Análise Espectral/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA