RESUMO
In the silkworm (Bombyx mori), tolerance to fluoride and scaleless wings are controlled by the dominant gene Dtf (dominant tolerance to fluoride) and recessive gene nlw (no Lepidoptera wings), respectively, and these genes have been mapped by using simple sequence repeat and sequence tag site markers. Marker-assisted evaluation and selection of silkworms with fluoride tolerance and scaleless wings were used for predicting fluoride resistance and scaleless wings in backcrossed animals. A silkworm strain was bred using this method, and its economic characteristics were found to be similar to those of commercial silkworms. These methods will therefore be useful for silkworm breeding programs and in screening for two or more characteristics of interest for segregating populations.
Assuntos
Bombyx/genética , Poluentes Ambientais/toxicidade , Fluoreto de Sódio/toxicidade , Animais , Sequência de Bases , Bombyx/anatomia & histologia , Bombyx/efeitos dos fármacos , Cruzamento , Tolerância a Medicamentos/genética , Feminino , Genes de Insetos , Ligação Genética , Marcadores Genéticos , Homozigoto , Endogamia , Masculino , Repetições de Microssatélites , Seda , Asas de Animais/anatomia & histologiaRESUMO
In the silkworm (Bombyx mori), resistance to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is linked to 7 simple-sequence repeat markers. Marker-assisted evaluation and selection of DNV-Z-resistant silkworms were used for predicting DNV-resistance in backcrossed animals. A silkworm race was bred using this method, and its economic characteristics were found to be similar to those of commercial silkworm races. These markers will therefore be useful for silkworm breeding programs and in screening for densonucleosis resistance in segregating populations.