RESUMO
BACKGROUND: The objective of the present study was to evaluate in vitro the cytotoxicity and bioactivity of various endodontic sealers (CeraSeal, BioRoot™ and AH Plus®) in pre-osteoblast mouse cells (MC3T3 cells). METHODS: MC3T3 cells (ATCC CRL-2594) were plated in 1 × 104 cells/well in 96-well plates in contact with endodontic sealers at concentrations of 1:10 and 1:100. Cell viability was evaluated by MTT assay after 24 and 48 h. In addition, sealer bioactivity was measured by RT-PCR for mediator of inflammation (Tnf, Ptgs2) and mineralization (Runx2, Msx1, Ssp1 and Dmp1) after 24 h and by Alizarin Red S Assay of mineralization after 28 days. Data were analyzed using one-way ANOVA followed by the Tukey's post-test at a significance level of 5%. RESULTS: BioRoot™ presented 24-hour cytotoxicity (p < 0.05) at 1:10 concentration. In the period of 48 h, no endodontic cement was cytotoxic to the cells compared to the control (p > 0.05). TNF-α gene expression was induced by AH Plus® (p < 0.05), while Ptgs2 was induced by the CeraSeal and BioRoot™ (p < 0.05). The expression of Runx2 was stimulated by BioRoot™ and AH Plus® (p < 0.05). In contrast, the expression of Dmp-1 Dmp1 was higher for the CeraSeal and BioRoot™ (p < 0.05). Nonetheless, the sealers did not impact the formation of mineralization nodules (p > 0.05). CONCLUSION: CeraSeal, BioRoot™ and AH Plus® sealers were not cytotoxic to MC3T3 cells within 48 h, but differentially induced the expression of genes related to inflammation and mineralization without impacting biomineralization by the cells.
Assuntos
Subunidade alfa 1 de Fator de Ligação ao Core , Materiais Restauradores do Canal Radicular , Camundongos , Animais , Teste de Materiais , Ciclo-Oxigenase 2 , Materiais Restauradores do Canal Radicular/toxicidade , Resinas Epóxi , Osteoblastos , InflamaçãoRESUMO
An experiment was conducted to evaluate the effect of the intake of a mixture of fish and sacha inchi oils (iOM), organic selenium (iSe), and organic chromium (iCr) on egg production (EP) and feed conversion ratio (FCR) of Isa Brown second-cycle laying hens (SCLH) for 16 weeks (91-106 weeks old). Egg production and FCR were evaluated using multivariate models that included conventional equations and artificial neural networks (ANN) to study multiple nutritional interactions as alternatives to univariate dose-response models. Based on the best models, iOM, iSe, and iCr levels were optimized, and a global sensitivity analysis was implemented to quantify their influence on EP and FCR. The modified logistic model was selected as the best strategy to represent EP. In the case of FCR, an ANN model with a feed-forward architecture and softmax transfer function was selected as the best alternative. One of the scenarios to simultaneously optimize EP (89.1%) and FCR (1.94 kg feed/kg egg) at 16 weeks of production was established with 3.3 g/hen·day of iOM, 0.132 mg/ hen·day of iSe, and 0.176 mg/hen·day of iCr. However, optimization considering only FCR results in much lower optimal iCr levels (between 0.083 and 0.105 mg/hen·day) with a slight decrease in EP (87.9%). The global sensitivity analysis showed that iSe is an essential factor associated with the increase in EP, and iCr is the most influential factor for the decrease in FCR. When both criteria were taken into account simultaneously from a desirability function, iSe was the most critical factor.(AU)
Assuntos
Animais , Selênio/efeitos adversos , Galinhas/fisiologia , Cromo/efeitos adversos , Ácidos Graxos Insaturados/efeitos adversos , Fenômenos Fisiológicos da Nutrição Animal , Análise MultivariadaRESUMO
COVID-19 pandemic has challenged public health systems worldwide, particularly affecting developing countries in Latin America like Ecuador. In this report, we exposed the fundamental role of the Ecuadorian universities to improve COVID-19 surveillance in the country, with an overall contribution over 15% of the total SARS-CoV-2 RT-PCR tests done. We highlight the role of our university during the first semester of the COVID-19 pandemic, contributing to a massive free SARS-CoV-2 testing up to almost 10% of the total diagnosis completed in the country, mainly focus on underserved urban, rural and indigenous communities. Finally, we described our contribution to a high quality and low-cost SARS-CoV-2 RT-PCR diagnostic in Ecuador.
RESUMO
An experiment with 23 diets was performed to evaluate the effect of digestible lysine (Lys), digestible methionine + cysteine (Met+Cys), and digestible threonine (Thr) on egg production of H&N Brown second-cycle laying hens (SCLH) for 20 weeks (92-111 weeks of age) in cages under environmental conditions. Body weight (BW), feed intake (FI), feed conversion ratio (FCR), egg weight (EW), number of hen-housed eggs, and livability were also evaluated during the experiment. Diets were formulated from a central composite design that combined five levels of Lys, Met+Cys, and Thr ranging from 727 to 1159, 662 to 1055, and 552 to 882 mg/kg, respectively. Egg production (EP) data were evaluated through three different modeling strategies: egg production models, multivariate polynomial models, and artificial neural networks (ANN). A cascade-forward neural network with logsigmoid transfer function was selected as the best model according to goodness-offit statistics in both identification and validation data. One of the best scenarios for EP of H&N Brown SCLH under specific outdoor conditions was established at Lys, Met+Cys, and Thr levels of 1138, 1031, and 717 mg/hen·day, respectively. The ANN model may be an appropriate tool to study and predict EP of H&N Brown SCLH based on the combination of three different levels of essential digestible amino acids. The strategies included in this work may contribute to improving poultry performance based on modeling techniques to study other production parameters in terms of different nutritional requirements and productive conditions.
Assuntos
Animais , Feminino , Galinhas , Dieta , Ovos , Aminoácidos Essenciais , Treonina , Dinâmica não Linear , Cisteína , Lisina , MetioninaRESUMO
Despite efforts made over decades by the Peruvian government to eliminate malaria, Plasmodium vivax remains a challenge for public health decision-makers in the country. The uneven distribution of its incidence, plus its complex pattern of dispersion, has made ineffective control measures based on global information that lack the necessary detail to understand transmission fully. In this sense, population genetic tools can complement current surveillance. This study describes the genetic diversity and population structure from September 2012 to March 2015 in three geographically distant settlements, Cahuide (CAH), Lupuna (LUP) and Santa Emilia (STE), located in the Peruvian Amazon. A total 777 P. vivax mono-infections, out of 3264, were genotyped. Among study areas, LUP showed 19.7% of polyclonal infections, and its genetic diversity (Hexp) was 0.544. Temporal analysis showed a significant increment of polyclonal infections and Hexp, and the introduction and persistence of a new parasite population since March 2013. In STE, 40.1% of infections were polyclonal, with Hexp = 0.596. The presence of four genetic clusters without signals of clonal expansion and infections with lower parasite densities compared against the other two areas were also found. At least four parasite populations were present in CAH in 2012, where, after June 2014, malaria cases decreased from 213 to 61, concomitant with a decrease in polyclonal infections (from 0.286 to 0.18), and expectedly variable Hexp. Strong signals of gene flow were present in the study areas and wide geographic distribution of highly diverse parasite populations were found. This study suggests that movement of malaria parasites by human reservoirs connects geographically distant malaria transmission areas in the Peruvian Amazon. The maintenance of high levels of parasite genetic diversity through human mobility is a critical barrier to malaria elimination in this region.
Assuntos
Transmissão de Doença Infecciosa , Genótipo , Malária Vivax/epidemiologia , Malária Vivax/parasitologia , Plasmodium vivax/classificação , Plasmodium vivax/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Técnicas de Genotipagem , Humanos , Incidência , Lactente , Recém-Nascido , Estudos Longitudinais , Malária Vivax/transmissão , Masculino , Repetições de Microssatélites , Pessoa de Meia-Idade , Epidemiologia Molecular , Peru/epidemiologia , Plasmodium vivax/isolamento & purificação , Adulto JovemRESUMO
PURPOSE: The objective of this paper is to report the clinical case of a patient who presented a chronic apical periodontitis, arising from internal inflammatory resorption followed by pulp necrosis, and a long-term success of a root canal therapy using calcium hydroxide as root canal dressing. CASE DESCRIPTION: A 20-year-old male patient presented for routine dental treatment. By radiographic examination we noted an extensive radioluscent area, laterally to the permanent maxillary right lateral incisor, with possibility of communication with the lateral periodontium, suggestive of a chronic apical periodontitis. Due to external root resorption detection, we used a calcium hydroxide root canal dressing, changed every 15 days, for a period of 2 months. Root canal filling was performed using gutta-percha cones by lateral condensation technique Radiographic follow up held after 19 years of treatment indicated a periodontium in conditions of normality, with the presence of lamina dura. CONCLUSION: Calcium hydroxide is a suitable material to be used as root canal dressing in teeth with apical periodontitis. Long-term evaluation demonstrated the satisfactory clinical outcome following root canal treatment.
OBJETIVO: O objetivo deste trabalho é relatar o caso clínico de um paciente que apresentava lesão periapical crônica decorrente de necrose de pulpar e o sucesso a longo prazo de uma terapia endodôntica utilizando o hidróxido de cálcio como curativo de demora. DESCRIÇÃO DO CASO: O paciente, do gênero masculino, com 20 anos de idade, compareceu à Clínica Odontológica para tratamento de rotina. Por meio de exames radiográficos observamos uma área radioluscente extensa, lateralmente ao incisivo lateral superior direito, com possibilidade de comunicação com o periodonto lateral, sugestivo de lesão periapical. Devido à presença de reabsorção radicular externa, foi utilizado um curativo de demora à base de hidróxido de cálcio (Calen®), trocado a cada 15 dias, por um período de 2 meses. A obturação do canal radicular foi realizada com cones de guta-percha pela técnica de condensação lateral ativa. O acompanhamento radiográfico realizado após 19 anos do tratamento endodôntico indicou um periodonto em condições de normalidade, com integridade da lamina dura. CONCLUSÃO: O hidróxido de cálcio é um material adequado para ser usado como curativo de demora em dentes com lesão periapical, uma vez que a avaliação a longo prazo demonstrou resultados clínicos satisfatórios após o tratamento endodôntico.
Assuntos
Humanos , Masculino , Adulto Jovem , Hidróxido de Cálcio/uso terapêutico , Tecido Periapical/lesões , Tratamento do Canal RadicularRESUMO
Congenital pathologies are those existing at or dating from birth. Occurrence of congenital cystic lesions in the oral cavity is uncommon in neonates. Eruption cyst (EC) is listed among these unusual lesions. It occurs within the mucosa overlying teeth that are about to erupt and, according to the current World Health Organization (WHO) classification of epithelial cysts of the jaws, EC is a separate entity. This paper presents a case of congenital EC successfully managed by close monitoring of the lesion, without any surgical procedure or tooth extraction. Eruption of the teeth involved, primary central incisors, occurred at the fourth month of age. During this time neither the child nor mother had any complication such as pain on sucking, refusal to feed, airway obstruction, or aspiration of fluids or teeth.
Assuntos
Doenças do Recém-Nascido/patologia , Doenças Mandibulares/patologia , Cistos Odontogênicos/patologia , Humanos , Lactente , Recém-Nascido , Masculino , Remissão Espontânea , Erupção DentáriaRESUMO
Congenital pathologies are those existing at or dating from birth. Occurrence of congenital cystic lesions in the oral cavity is uncommon in neonates. Eruption cyst (EC) is listed among these unusual lesions. It occurs within the mucosa overlying teeth that are about to erupt and, according to the current World Health Organization (WHO) classification of epithelial cysts of the jaws, EC is a separate entity. This paper presents a case of congenital EC successfully managed by close monitoring of the lesion, without any surgical procedure or tooth extraction. Eruption of the teeth involved, primary central incisors, occurred at the fourth month of age. During this time neither the child nor mother had any complication such as pain on sucking, refusal to feed, airway obstruction, or aspiration of fluids or teeth.
Patologias congênitas são aquelas que aparecem ao nascimento. A ocorrência de lesões císticas congênitas na cavidade bucal é rara em recém-nascidos. O cisto de erupção (CE) pode ser considerado como lesão rara que se localiza na mucosa que recobre um dente que está próximo do momento de sua erupção. De acordo com a classificação de cistos epiteliais dos maxilares, descrita pela Organização Mundial da Saúde (OMS), os CE são considerados uma entidade distinta. O presente relato descreve um caso clínico de um cisto congênito de erupção tratado com sucesso somente pelo acompanhamento da lesão, sem a necessidade de nenhuma intervenção cirúrgica ou extração dentária. A erupção dos dentes envolvidos (incisivos centrais decíduos) ocorreu aos 4 meses de idade e durante este período não houve reclamações da mãe e/ou da criança em relação a dor para sucção, recusa para se alimentar, obstrução das vias aéreas e aspiração de fluidos ou de dentes.
Assuntos
Humanos , Lactente , Recém-Nascido , Masculino , Doenças do Recém-Nascido/patologia , Doenças Mandibulares/patologia , Cistos Odontogênicos/patologia , Remissão Espontânea , Erupção DentáriaRESUMO
Hymenoptera order includes bees, which have a stinging apparatus at the tail capable of delivering venom to the affected tissues. Myocardial infarction, acute renal failure, Necrotizing fasciitis, fatal infection and hemifacial asymmetry, are some of the unusual reactions reported following hymenoptera stings. This paper reports a case of bee sting in the right floor of the nose that mimicked an odontogenic infection affecting the upper lip, canine space and nasal cavity such as in cases of infection secondary to pulpal or periodontal pathology of the anterior teeth. After a thorough clinical and radiographic examination, odontogenic infection was discarded and the diagnosis of floor of the nose mucosal lysis and lip abscess secondary to a bee sting was made. This case was successfully managed with adequate incision, drainage and antibiotics without any further complication. There are several reports of unusual reactions following hymenoptera stings. However, just a few of them referred to infections of local reactions and none of them related to the anatomic location affected in the patient of the present case. Early diagnosis and treatment prevented infection dissemination and the likelihood of tissue necrosis as in previously reported cases of Necrotizing fasciitis.
Assuntos
Abscesso/patologia , Fístula Dentária/patologia , Mordeduras e Picadas de Insetos/complicações , Doenças Labiais/patologia , Mucosa Nasal/patologia , Abscesso/etiologia , Abscesso/terapia , Adulto , Animais , Venenos de Abelha/efeitos adversos , Abelhas , Desbridamento , Fístula Dentária/etiologia , Fístula Dentária/terapia , Diagnóstico Diferencial , Drenagem , Humanos , Doenças Labiais/etiologia , Doenças Labiais/terapia , Masculino , Doenças Dentárias/patologia , Resultado do TratamentoRESUMO
Sodium hypochlorite solution is toxic to vital tissues, causing severe effects if extruded during endodontic treatment. This paper presents a report on the tissue damage related to inadvertent extrusion of concentrated sodium hypochlorite solution during root canal treatment. A 65-year-old woman was referred with moderate pain, ecchymosis, and severe swelling of the right side of the face. These symptoms appeared immediately after a root canal treatment of the maxillary right canine, which had been started 21 hours earlier. It was diagnosed as air emphysema related to sodium hypochlorite solution extravasation during the endodontic treatment. To avoid this, an initial radiograph should be taken to determine the correct canal working length and confirm root canal integrity.
Assuntos
Extravasamento de Materiais Terapêuticos e Diagnósticos/etiologia , Tecido Periapical/lesões , Irrigantes do Canal Radicular/efeitos adversos , Preparo de Canal Radicular/efeitos adversos , Hipoclorito de Sódio/efeitos adversos , Idoso , Fístula Dentária/etiologia , Equimose/induzido quimicamente , Edema/induzido quimicamente , Feminino , Humanos , Abscesso Periapical/etiologia , Tecido Periapical/efeitos dos fármacos , Enfisema Subcutâneo/induzido quimicamenteRESUMO
Hymenoptera order includes bees, which have a stinging apparatus at the tail capable of delivering venom to the affected tissues. Myocardial infarction, acute renal failure, Necrotizing fasciitis, fatal infection and hemifacial asymmetry, are some of the unusual reactions reported following hymenoptera stings. This paper reports a case of bee sting in the right floor of the nose that mimicked an odontogenic infection affecting the upper lip, canine space and nasal cavity such as in cases of infection secondary to pulpal or periodontal pathology of the anterior teeth. After a thorough clinical and radiographic examination, odontogenic infection was discarded and the diagnosis of floor of the nose mucosal lysis and lip abscess secondary to a bee sting was made. This case was successfully managed with adequate incision, drainage and antibiotics without any further complication. There are several reports of unusual reactions following hymenoptera stings. However, just a few of them referred to infections of local reactions and none of them related to the anatomic location affected in the patient of the present case. Early diagnosis and treatment prevented infection dissemination and the likelihood of tissue necrosis as in previously reported cases of Necrotizing fasciitis.
A ordem das himenópteras inclui abelhas, que possuem um ferrão na cauda capaz de de injetar veneno nos tecidos afetados. Fasciite necrosante, infecção fatal e assimetria hemifacial são algumas das reações incomuns relatadas após picada de himenópteras. Este estudo relata um caso de picada de abelha no assoalho da narina direita que simulou uma infecção odontogênica atingindo o lábio superior, o espaço canino e a cavidade nasal, como observado nos casos de infecção secundária após patologia pulpar ou periodontal dos dentes anteriores. Após completo exame clínico e radiográfico, foi descartada a hipótese de infecção odontogênica e foi estabelecido o diagnóstico de lise da mucosa do assoalho da narina e abscesso do lábio, após picada de abelha. Este caso foi tratado com sucesso com adequada incisão, drenagem e administração de antibióticos, sem outras complicações. Há vários relatos de reações incomuns resultantes de picada de himenópteras, porém poucos são relativos a infecções locais e nenhum se refere à localização anatômica afetada no paciente do caso aqui relatado. Diagnóstico e tratamento precoces preveniram a disseminação da infecção e a possibilidade de necrose tecidual, como já relatado em casos de fasciite necrosante.
Assuntos
Adulto , Animais , Humanos , Masculino , Abscesso/patologia , Fístula Dentária/patologia , Mordeduras e Picadas de Insetos/complicações , Doenças Labiais/patologia , Mucosa Nasal/patologia , Abscesso/etiologia , Abscesso/terapia , Abelhas , Venenos de Abelha/efeitos adversos , Desbridamento , Diagnóstico Diferencial , Drenagem , Fístula Dentária/etiologia , Fístula Dentária/terapia , Doenças Labiais/etiologia , Doenças Labiais/terapia , Resultado do Tratamento , Doenças Dentárias/patologiaRESUMO
This paper describes a case of autogenous tooth transplantation. An external inflammatory root resorption developed in a 30-year-old female patient and was successfully treated with the placement of calcium hydroxide-based intracanal dressings. Autotransplantation of tooth 32 into the extraction socket of tooth 30 was carried out with no intraoperative complications and uneventful postoperative course. However, the patient did not appear on the 14th postoperative day to initiate endodontic therapy of the transplanted tooth. She did not return for 3 months, at which time the clinical and periapical radiographic examinations revealed the presence of external inflammatory root resorption. Endodontic treatment was initiated immediately. Root canals were prepared and filled with a calcium hydroxide-based intracanal dressing (Calen paste), changed every 21 days during a 6-month period, and were thereafter obturated with Sealapex calcium hydroxide-based sealer and gutta-percha points. After 2 years of follow-up, the transplanted tooth was symptom free with no exacerbated sensibility to percussion, normal mobility, occlusion, and masticatory function. Probing revealed no periodontal pockets or other pathological signs. Radiographic examination showed the periradicular area with normal appearance, completely healed resorption areas, and presence of an intact lamina dura.
Assuntos
Dente Serotino/transplante , Procedimentos Cirúrgicos Bucais/efeitos adversos , Tratamento do Canal Radicular/métodos , Reabsorção da Raiz/terapia , Alvéolo Dental/cirurgia , Adulto , Hidróxido de Cálcio , Feminino , Guta-Percha , Humanos , Materiais Restauradores do Canal Radicular/uso terapêutico , Irrigantes do Canal Radicular/uso terapêutico , Reabsorção da Raiz/etiologia , Salicilatos , Transplante Autólogo/efeitos adversosRESUMO
This case report describes the nonsurgical endodontic treatment and intentional replantation of a mandibular molar and its nearly 14-year follow-up. A 56-year-old woman sought treatment with complaint of discomfort and sensitivity in the mandibular left area. The radiographic examination showed that the mandibular left first molar was endodontically treated, with over-instrumentation of the distal root and unsatisfactory obturation on the mesial root. A large periapical lesion was observed surrounding the roots. In view of the patient's limited interocclusal space and objection to undergo an apical surgery, intentional replantation was suggested as an alternative. The patient returned for clinical and radiographic follow-up at 15 days, 40 days, 6 months, 12 months, and once yearly thereafter. At the last visit, after 14 years, no painful symptomatology was reported, probing depth was no greater than 3 mm, the periradicular area had normal appearance and no evidence of root resorption or periapical lesion.