1.
Cell Mol Biol (Noisy-le-grand)
; 69(7): 19-23, 2023 Jul 31.
Artigo
em Inglês
| MEDLINE
| ID: mdl-37715442
RESUMO
Simplexvirus humanalpha1 (HSV-1) aï¬ects approximately 67% of the world's population. Here, we sought to use the CRISPR / Cas9 system with the UL39 target, essential for virus replication. The sgRNA sequence was inserted into the plasmid (PX459-UL39). Vero cells were transfected with PX459-UL39, and inhibition of viral replication was assessed 24 and 48 hours later using plaque assays and ï¬uorescence and qPCR. Fluorescence analyses revealed the presence of anti-HSV-1 CRISPR/Cas9 within Vero cells, and qPCR showed that the viral load decreased by> 95% of cells transfected with anti-HSV-1 CRISPR / Cas 9. Our data demonstrate the usefulness of the PX459-UL39 to inhibit HSV-1 infection.