RESUMO
PURPOSE OF THE STUDY: determine the sensitivity and specificity of magnetic resonance imaging in anterior cruciate ligament injuries and associated injuries through arthroscopic findings. MATERIAL AND METHODS: this is a retrospective, longitudinal, cross-sectional study in 96 patients with ACL injuries were included and who underwent arthroscopic surgery; arthroscopic findings were compared with diagnostic magnetic resonance images as well as associated lesions. RESULTS: for ACL lesions the following data were found in relation to the agreement by MRI and arthroscopic findings; 93.68% sensitivity, 100% specificity. Negative predictor value of 14.28% and a positive predictor value of 100%. CONCLUSION: MRI is an accurate and non-invasive imaging modality for the evaluation of knee injuries, the diagnostic association is considerably high.
PROPÓSITO DEL ESTUDIO: determinar la sensibilidad y especificidad de la resonancia magnética en lesiones de ligamento cruzado anterior, así como las lesiones asociadas mediante los hallazgos artroscópicos. MATERIAL Y MÉTODOS: se trata de un estudio retrospectivo, longitudinal, transversal en el cual se incluyeron 96 pacientes con lesiones de ligamento cruzado anterior (LCA) que fueron sometidos a cirugía artroscópica; los hallazgos artroscópicos se compararon con las imágenes diagnósticas de resonancia magnética así como lesiones asociadas. RESULTADOS: en lesiones de LCA se encontraron los siguientes datos en relación a la concordancia por resonancia magnética nuclear (RMN) y hallazgos artroscópicos; sensibilidad de 93.68%, especificidad de 100%. Valor predictor negativo de 14.28% y un valor predictor positivo de 100%. CONCLUSIÓN: la RMN es una modalidad de imagen precisa y no invasiva para la evaluación de lesiones de la rodilla, la asociación diagnóstica con el examen clínico es considerablemente alta.
Assuntos
Lesões do Ligamento Cruzado Anterior , Humanos , Lesões do Ligamento Cruzado Anterior/diagnóstico por imagem , Lesões do Ligamento Cruzado Anterior/cirurgia , Ligamento Cruzado Anterior/patologia , Ligamento Cruzado Anterior/cirurgia , Estudos Retrospectivos , Estudos Transversais , Imageamento por Ressonância Magnética/métodos , ArtroscopiaRESUMO
Prodiplosis longifila is reported as a pest of a wide range of species cultivated in America, including citrus, solanaceous species and asparagus. This species has different behavioural traits that are primarily centred on the oviposition habit and the feeding of larvae, which can change depending on the host. However, scarce information is available on population studies and the natural history of this insect, and uncertainty exists about the taxonomic identity and the geographic distribution of this species. The main objective was to perform a phylogenetic and genetic study of P. longifila populations and to define whether the North American and South American populations belong to the same species or whether a differentiation process had occurred due to geographic distance. A second objective was to determine whether this species showed genetic differentiation by host specialization in South America. The phylogenetic and population analyses based on DNA barcodes (cytochrome oxidase I gene) and a region of the ribosomal DNA (ITS2) revealed divergent clades attributable to geographic distance and host specificity. The North American and South American P. longifila insects were confirmed to be genetically distinct, and the genetic distances exceeded the values expected for intraspecific variation. In South America, the population analysis of P. longifila from tomato, sweet pepper (Solanaceae), Tahiti lime and key lime (Rutaceae) hosts evidenced high genetic differentiation between populations associated with different hosts and an absence of gene flow between these groups, suggesting the corresponding formation of cryptic species.
Assuntos
Dípteros/genética , Evolução Molecular , Especiação Genética , Herbivoria , Distribuição Animal , Animais , Colômbia , DNA Mitocondrial/análise , DNA Espaçador Ribossômico/análise , Equador , Complexo IV da Cadeia de Transporte de Elétrons/análise , Florida , Filogenia , FilogeografiaRESUMO
Airway smooth muscle (ASM) membrane depolarization through KCl opens L-type voltage dependent Ca2+ channels (Ca(v)1.2); its opening was considered the cause of KCl contraction. This substance is used to bypass intracellular second messenger pathways. It is now clear that KCl also activates RhoA/Rho kinase (ROCK) pathway. ROCK isoforms are characterized as ROCK1 and ROCK2. Because ROCK1 seems the most abundant isotype in lung, we studied its participation in KCl stimulated bovine ASM. With methyl-beta-cyclodextrin (MbetaCD) we disrupted caveolae, a membrane compartment considered as the RhoA/ROCK assembly site, and found that KCl contraction was reduced to the same extent (~26%) as Y-27632 (ROCK inhibitor) treated tissues. We confirmed that KCl induces ROCK activation and this effect was annulled by Y-27632 or MbetaCD. In isolated plasmalemma, ROCK1 was localized in non-caveolar membrane fractions in Western blots from control tissues, but it transferred to caveolae in samples from tissues stimulated with KCl. Ca(v)1.2 was found at the non-caveolar membrane fractions in control and MbetaCD treated tissues. In MbetaCD treated tissues stimulated with KCl, contraction was abolished by nifedipine; only the response to Ca(v)1.2 opening remained as the ROCK component disappeared. Our results show that, in ASM, the KCl contraction involves the translocation of ROCK1 from non-caveolar to caveolar regions and that the proper physiological response depends on this translocation.
Assuntos
Cavéolas/metabolismo , Contração Muscular/fisiologia , Músculo Liso/metabolismo , Cloreto de Potássio/farmacologia , Traqueia/metabolismo , Quinases Associadas a rho/metabolismo , Animais , Bovinos , Cavéolas/efeitos dos fármacos , Contração Muscular/efeitos dos fármacos , Relaxantes Musculares Centrais/farmacologia , Músculo Liso/efeitos dos fármacos , Técnicas de Cultura de Órgãos , Transporte Proteico/efeitos dos fármacos , Transporte Proteico/fisiologia , Traqueia/efeitos dos fármacosRESUMO
Chiari malformation type I and Poland's syndrome are two rare diseases and their simultaneous presentation had not been previously described in the literature. We report the case of a 27 year old male with history of Poland's syndrome, who referred headache and motor impairment of the intrinsic muscles of the left hand. In a cervical spine MR a Chiari I malformation with syringomyelia from C1 to T2 was found, which was treated by foramen magnum decompression, dural plasty and removal of the posterior arch of the atlas. A discussion of the embryological mechanisms that might be involved in the coexistence of these two entities is presented, emphasizing the role of para-axial mesoderm.
Assuntos
Malformação de Arnold-Chiari/epidemiologia , Síndrome de Poland/epidemiologia , Adulto , Malformação de Arnold-Chiari/etiologia , Malformação de Arnold-Chiari/cirurgia , Comorbidade , Humanos , Imageamento por Ressonância Magnética , Masculino , Síndrome de Poland/etiologia , Síndrome de Poland/cirurgiaRESUMO
Clear cell meningioma is a rare variety of meningiomas, occurring frequently at the cerebellopontine angle and spinal canal. A case of a female patient 28 years of age with a complete cauda equina syndrome was described. Magnetic resonance imaging of lumbo-sacral spine revealed a mass lesion occupying the spinal canal from L3 to S1. Subtotal resection was performed and pathological pathological examination revealed a clear cell meningioma. Radiotherapy was indicated, however, it was delayed because we discovered that the patient was pregnant. The recurrence was clinically evident at seven months and a new surgical resection previous complementary radiotherapy was necessary. A systematic review of literature was performed, exposing the behavior of clear cell meningioma in the spinal canal.
Assuntos
Neoplasias Meníngeas/patologia , Meningioma/patologia , Adulto , Feminino , Humanos , Região Lombossacral/patologia , Imageamento por Ressonância Magnética , Neoplasias Meníngeas/diagnóstico , Neoplasias Meníngeas/cirurgia , Meningioma/diagnóstico , Meningioma/cirurgia , Recidiva Local de Neoplasia/radioterapia , Recidiva Local de Neoplasia/cirurgia , Polirradiculopatia , GravidezRESUMO
Metastases of prostate cancer to intracranial meninges are rare and often confused with meningiomas or chronic subdural hematomas. These usually occur in patients with a known cancer diagnosis in advanced stages of the disease, and only in some rare cases do its manifestations precede the detection of the primary tumour. The clinical presentation is unspecific. However, due to the affinity of this tumour for the base of the skull, it must be included in the differential diagnosis of men over 70 years of age with cranial nerve palsy. The treatment of these lesions has not been standardized. Within the therapeutic options we find surgical resection, chemotherapy, radiotherapy or a combination of these measures, and yet survival is poor. We present the case of a 77 year old male patient whose initial symptoms of prostate cancer were caused by a metastatic lesion to the dura, confirmed by histopathology. We also review the epidemiological, clinical and imaging highlights of this condition.
Assuntos
Adenocarcinoma/secundário , Dura-Máter , Neoplasias Meníngeas/secundário , Neoplasias da Próstata/patologia , Idoso , Humanos , MasculinoRESUMO
Three experiments were carried out to determine the optimum selenium-vitamin E injection level to maintain acceptable blood selenium (Se) status of does and kids, as well as to determine the relation of that status to mortality rates in kids. In experiment 1, 238 goats were assigned to one of three groups during the mating period: A1-control, A2- 0.06mgSe+0.8IU vitE/kgBW and A3-0.125mgSe+1.7IU vitE/kgBW. No differences (P>.05) for fertility and prolificacy were observed among the groups. Blood Se concentration did not differ among Se-vit E groups and control group before injection, and both groups showed Se deficient condition. There was a trend (P<.05, 32%) to increase Se blood level 60 days post-treatment, but difference was not observed between A2 and control groups, while difference (P<.05; 103%) was observed between A1 and A2 groups vs high Se injection (A3 group). In experiment 2, 48 goats were divided into four groups: B1- control, B2- 0.125mgSe+1.7IU vitE/kgBW, B3- 0.25mgSe+3.4IU vitE/kgBW and B4- 0.31mgSe+4.2IU vitE/kgBW. The B4 group reached the highest concentration at the third month after injection (0.11 ppm), then started to decline after 100 days, reaching a value slightly higher than B2 and B3 on the 135th day of pregnancy. Results of B2 and B3 groups were slightly higher that those of B1 (P<0.05). In experiment 3, 194 kids (3 to 7 days postpartum) born from Se-deficient goats were used to compare the effectiveness of Se injection. They were divided into three groups: C1- control, C2- 0.3mgSe+4.2IU vit E/kg BW and C3- 0.6mgSe+8.4IU vit E/kg BW. C1 showed the highest percentage of mortality (60%) as compared to treated Se groups, that scored equal percentage of deaths (22% averaged). The concentration of Se in blood, on day 20th after the onset of the treatment rapidly increased, according with level of Se injection.(AU)
Foram conduzidos três experimentos para determinar a quantidade ótima da injeção de selênio-vitamina E (Se-vit E) para manter o nível aceitável do status de selênio no sangue de cabras e cabritos e determinar essa relação com o status sobre a taxa de mortalidade de cabritos. No primeiro experimento, 238 cabras foram usados em um dos três grupos durante o período do acasalamento: A1- controle, A2- 0,06mgSe+0,8UI vit E/kg de peso-vivo e A3- 0,125mgSe+1,7UI vitE/kg de peso-vivo. Não houve diferenças (P>0,05) entre os grupos para fertilidade e prolificidade. A concentração de Se no sangue não foi diferente entre os grupos que receberam Se-vit E e o controle antes da injeção e ambos os grupos mostravam deficiência de Se. Sessenta dias pós-tratamento houve tendência (P<0,05; 32%) de aumentar o nível de Se no sangue e não houve diferença entre os grupos A2 e controle. Houve diferença significativa (P<0,05; 103%) entre os grupos A1 e A2 e o grupo A3. No segundo experimento, 48 cabras foram divididas em quatro grupos: B1- controle, B2- 0,125mgSe+1,7UI vitE/kg de peso-vivo, B3- 0,25mgSe+3,4UI vitE/kg de peso-vivo e B4- 0,31mgSe+4,2UI vitE/kg de peso-vivo. O grupo B4 alcançou a concentração mais alta no terceiro mês depois da injeção (0,11ppm) e diminuiu depois de 100 dias, quando o valor foi apenas ligeiramente maior que os valores dos grupos B2 e B3, aos 135 dias da gestação. Os resultados dos grupos B2 e B3 foram ligeiramente mais altos que os do grupo B1 (P<0,05). No terceiro experimento, 194 cabritos (3 a 7 dias de idade), nascidos de cabras deficientes em Se, foram usados para comparar a efetividade da injeção de Se. Foram divididos em três grupos: C1- controle, C2- 0,3mgSe+4,2UI vit E/kg de peso-vivo e C3- 0,6mgSe+8,4UI vit E/kg de peso-vivo.A porcentagem de mortalidade foi mais alta em C1 (60%) e mais baixa nos grupos tratados com Se (22%).A concentração de Se no sangue no 20º após o início do tratamento aumentou rapidamente, de acordo com o nível de injeção de Se.(AU)
Assuntos
Animais , Feminino , Cabras/crescimento & desenvolvimento , Cabras/sangue , Selênio/administração & dosagem , Vitamina E/administração & dosagem , Doença do Músculo Branco , Prenhez , Animais Recém-Nascidos/crescimento & desenvolvimento , Animais Recém-Nascidos/sangueRESUMO
Foram conduzidos três experimentos para determinar a quantidade ótima da injeção de selênio-vitamina E (Se-vit E) para manter o nível aceitável do status de selênio no sangue de cabras e cabritos e determinar essa relação com o status sobre a taxa de mortalidade de cabritos. No primeiro experimento, 238 cabras foram usados em um dos três grupos durante o período do acasalamento: A1- controle, A2- 0,06mgSe+0,8UI vitE/kg de peso-vivo e A3- 0,125mgSe+1,7UI vitE/kg de peso-vivo. Não houve diferenças (P>0,05) entre os grupos para fertilidade e prolificidade. A concentração de Se no sangue não foi diferente entre os grupos que receberam Se-vit E e o controle antes da injeção e ambos os grupos mostravam deficiência de Se. Sessenta dias pós-tratamento houve tendência (P<0,05; 32%) de aumentar o nível de Se no sangue e não houve diferença entre os grupos A2 e controle. Houve diferença significativa (P<0,05; 103%) entre os grupos A1 e A2 e o grupo A3. No segundo experimento, 48 cabras foram divididas em quatro grupos: B1- controle, B2- 0,125mgSe+1,7UI vitE/kg de peso-vivo, B3- 0,25mgSe+3,4UI vitE/kg de peso-vivo e B4- 0,31mgSe+4,2UI vitE/kg de peso-vivo. O grupo B4 alcançou a concentração mais alta no terceiro mês depois da injeção (0,11ppm) e diminuiu depois de 100 dias, quando o valor foi apenas ligeiramente maior que os valores dos grupos B2 e B3, aos 135 dias da gestação. Os resultados dos grupos B2 e B3 foram ligeiramente mais altos que os do grupo B1 (P<0,05). No terceiro experimento, 194 cabritos (3 a 7 dias de idade), nascidos de cabras deficientes em Se, foram usados para comparar a efetividade da injeção de Se. Foram divididos em três grupos: C1- controle, C2- 0,3mgSe+4,2UI vitE/kg de peso-vivo e C3- 0,6mgSe+8,4UI vit E/kg de peso-vivo. A porcentagem de mortalidade foi mais alta em C1 (60%) e mais baixa nos grupos tratados com Se (22%). A concentração de Se no sangue no 20º após o início do tratamento aumentou rapidamente, de acordo com o nível de injeção de Se. A injeção com 0,3mgSe/kg de peso-vivo aumentou a concentração de Se no sangue em cabras gestantes e a dose foi efetiva para prevenir a doença de músculo branco e aumentar a sobrevivência dos cabritos até o desmame.
Assuntos
Animais , Feminino , Cabras/crescimento & desenvolvimento , Cabras/sangue , Selênio/administração & dosagem , Vitamina E/administração & dosagem , Animais Recém-Nascidos/crescimento & desenvolvimento , Animais Recém-Nascidos/sangue , Prenhez , Doença do Músculo BrancoRESUMO
A revision is made of the previously poorly studied blackfly fauna from the south-western border of Guyana with Brazil. Notes on the biosystematics of the species found are provided, together with keys and illustrations based on their morphology. Of the 14 species recorded, eight are anthropophilic and two of these (Simulium oyapockense s.l. and S. guianense s.l.) are proven vectors of human onchocerciasis in the nearby Amazonia focus of the disease in neighbouring Brazil.
Assuntos
Insetos Vetores/classificação , Simuliidae/classificação , Animais , Brasil , Feminino , Guiana , Humanos , Insetos Vetores/anatomia & histologia , Masculino , Oncocercose/transmissão , Pupa/anatomia & histologia , Simuliidae/anatomia & histologiaRESUMO
A revision is made of the previously poorly studied blackfly fauna from the south-western border of Guyana with Brazil. Notes on the biosystematics of the species found are provided, together with keys and illustrations based on their morphology. Of the 14 species recorded, eight are anthropophilic and two of these (Simulium oyapockense s.l. and S. guianense s.l.) are proven vectors of human onchocerciasis in the nearby Amazonia focus of the disease in neighbouring Brazil.
Assuntos
Humanos , Animais , Masculino , Feminino , Insetos Vetores , Simuliidae , Brasil , Guiana , Oncocercose , PupaRESUMO
During studies on the taxonomy of the Simuliidae of Brazil, a new species of Simulium was found. Full descriptions of the adults and pupae of this species are described here, its affinities with other species are discussed and its distribution, biology, and medical importance in Brazil are recorded.
Assuntos
Simuliidae/classificação , Animais , Brasil , Feminino , Geografia , Masculino , Simuliidae/anatomia & histologiaRESUMO
During studies on the taxonomy of the Simuliidae of Brazil, a new species of Simulium was found. Full descriptions of the adults and pupae of this species are described here, its affinities with other species are discussed and its distribution, biology, and medical importance in Brazil are recorded.
Assuntos
Animais , Masculino , Feminino , Simuliidae , Brasil , Geografia , SimuliidaeRESUMO
The causes of mortality in kids under extensive conditions in the Mexico plateau were studied. Perinatal mortality of kids (0-7 days) was lower (P<0.05) than mortality in the other age groups (8-90 days). The weights of the animals at death were extremely low, group (i) 0-7 days, 2.3kg; (ii) 7-30 days, 2.8kg and (iii) 30-90 days, 4kg. The principal finding at necropsy was white muscle disease (WMD). The WMD lesions, suggested selenium (Se) deficiency was present in 49 out of the 74 kids evaluated in this study (P<0.05). In many cases, muscular dystrophy was present with other pathological conditions. Enteric and pneumonic conditions were also important causes. In the small intestine villus atrophy was observed in 14 of the 25 kids that died with enteric problems between 8 and 30 days of age. Pneumonic lesions were present in 12 out of 74 kids. Miscellaneous conditions as starvation (five cases), hepatic conditions (three cases), omphalophlebitic infection (two cases) were also present in lower proportions. In conclusion, WMD was considered the main cause of deaths in goat kids between 8 and 90 days of age. Evaluation of Se status of soil, forages and goat tissues is recommended.
RESUMO
The objective of this study was to determine the concentration of Selenium (Se) in soils, forages and the tissues of kids and does under extensive grazing conditions in two regions of the Tlaxcala state, Mexico (Carrillo Puerto and Ixtenco) in the dry and rainy season. There were no differences in the concentration of Se in soil (0.051 and 0.047ppm), pH of the soil (6.1 and 5.9), concentration of Se in the forage (0.052 and 0.075ppm) and blood serum of goats (0.02 and 0.021ppm) during the dry and rainy seasons, respectively. The Carrillo Puerto region had a significantly higher content of Se in the soil (31%), soil pH (7%), content of Se in forage (25.9%), and content of Se in blood serum of goats (16%) compared to the corresponding values obtained in Ixtenco. The mean concentration of Se in the serum of kids with clinical signs of white muscle disease, was significantly lower (36.3%) compared to the kids from the same farm that were apparently healthy. The results of this study suggest that soil, forages, and goats have a marginal Se status in both regions during the dry and rainy seasons. Further research is required to find the most appropriate method to correct the Se-deficiency in goats from these regions.
RESUMO
Se presenta una nina con asimetria mandibular expresion clinica del llamado sindrome de Weyer o disostosis mandibulo facial unilateral. Se comenta sobre este sindrome