RESUMO

Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amino acid sequences showed a similarity of 88% between the respective viral coat proteins. The garlic potyvirus coat protein was expressed in E. coli cells, purified, and subjected to Western blot analysis using antibodies raised against different garlic-infecting viruses. The expression protein was consistently recognised by anti-OYDV antibodies and did not react with antibodies specific for leek yellow stripe potyvirus (LYSV), garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV). Besides, the garlic potyvirus coat protein was obtained as a fusion protein and used as antigen to produce polyclonal antibodies. These antibodies reacted with purified OYDV virions, but failed to recognise LYSV particles. In the light of this evidence the garlic potyvirus was identified as the garlic strain of OYDV.

Assuntos

Capsídeo/genética , Potyvirus/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Western Blotting , Capsídeo/imunologia , Capsídeo/metabolismo , Clonagem Molecular , Escherichia coli/genética , Alho/virologia , Expressão Gênica , Dados de Sequência Molecular , Cebolas/virologia , Plantas Medicinais , Potyvirus/imunologia , Potyvirus/metabolismo , Coelhos , Proteínas Recombinantes de Fusão/imunologia , Recombinação Genética , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos