RESUMO

A material isolated following pregnenolone incubations with toad (Bufo arenarum) inter-renal tissue at 28 degrees C has been identified as a 3 beta-hydroxy-5-ene analogue of aldosterone (3 beta, 11 beta, 21-trihydroxy-20-oxo-5-pregnen-18-al). The initial identification was made by enzymic and m.s. methods, and structural confirmation was achieved through comparison with chemically synthesized authentic material. The relative efficacy of corticosterone, 18-hydroxycorticosterone and the 3 beta-hydroxy-5-ene aldosterone analogue as aldosterone precursors was evaluated. In the in vitro situation studied, the 3 beta-hydroxy-5-ene steroid was by far the best precursor.

Assuntos

Aldosterona/análogos & derivados , Aldosterona/biossíntese , 3-Hidroxiesteroide Desidrogenases/metabolismo , Aldosterona/metabolismo , Animais , Bufo arenarum , Cromatografia Gasosa-Espectrometria de Massas , Rim/metabolismo