RESUMO
Yellowfin tuna by-products (Thunnus albacares) were processed to produce radical-scavenging peptides from hydrolysis by lactic acid fermentation (LAF) with Lactobacillus plantarum, papaya fruit (Carica papaya), and molasses as a carbon source for 72 h. A 15-kDa peptide was purified; after de novo sequencing, it was determined that fragments are rich in hydrophobic and neutral amino acids. The results suggest this effect is mainly to the hydrophobicity of the amino acids in their sequence. Further work is on progress to assess the ability of peptides to provide stability in lipids or in other types of samples sensitive to the action of free radicals.
Assuntos
Sequestradores de Radicais Livres , Ácido Láctico/metabolismo , Peptídeos , Análise de Sequência de Proteína , Atum , Animais , Carica/química , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Frutas , Lactobacillus plantarum/crescimento & desenvolvimento , Melaço , Peptídeos/química , Peptídeos/genética , Peptídeos/isolamento & purificaçãoRESUMO
OBJECTIVES: To measure in vitro intrapulpal temperature when placing and restoring with either room-temperature or pre-heated (54 and 60 degrees C) composite. METHODS: A K-type thermocouple was placed in the pulpal chamber of an extracted, human bifurcated upper premolar which had a Class V preparation (1 mm remaining dentin thickness) on the facial surface. Tooth roots were immersed in a thermostatically controlled water bath and perfused with water at 1.25 microl/min to simulate physiological circulation in the pulp chamber. The thermocouple was connected to an analog-to-digital converter. The preparation was filled using composite either at room-temperature, or pre-heated to 54 or 60 degrees C with a commercial compule heater (Calset), using standard clinical procedures by one person while continuously monitoring intrapulpal temperature (n=5). Temperature rise over baseline values were determined at various stages during the restoration process: composite placement, contouring, prior to light-curing, and immediately after light-curing (20s, Optilux 501). At each measurement interval, intrapulpal temperature values were compared using ANOVA and the Tukey-Kramer post hoc test (alpha=0.05). RESULTS: Significant differences were found in intrapulpal temperature when comparing pre-heated and room-temperature composite treatments with respect to baseline among the stages of the restorative process. However, the extent of this increase with heated composite was only 0.8 degrees C. A 5 degrees C intrapulpal temperature rise was seen for all groups during photopolymerization. SIGNIFICANCE: Use of pre-heated composite only mildly increased intrapulpal temperature values when compared to composite delivered at room-temperature in an in vitro test environment. The largest temperature change occurred with application of the curing light.
Assuntos
Resinas Acrílicas/química , Resinas Compostas/química , Polpa Dentária/química , Restauração Dentária Permanente/métodos , Poliuretanos/química , Análise de Variância , Temperatura Alta , HumanosRESUMO
From the results of the study, it is evident that the public's awareness of, and response to immunization has been below the required standard (AU)