RESUMO
BACKGROUND: At present, data about the cross-reactivity of Blomia spp. comes from studies made among different genera of mites, and no results have been published involving different species of the genus Blomia. OBJECTIVE: The aim of this study was to find out the level of cross-reactivity between the two main species of Blomia causing allergy, and its implication in the diagnosis of Blomia sensitization. METHODS: Using extracts from optimal growth phases of Blomia kulagini (Zakhvatkin, 1936) and Blomia tropicalis (van Bronswijk, Cock and Oshima, 1973) as allergenic material, the allergenic cross-reactivity between both house dust mites was evaluated by means of cutaneous tests, specific IgE values, ImmunoCAP-inhibition and SDS-PAGE-IgE-immunoblotting-inhibition. RESULTS: The results demonstrated that IgE-binding components belonging to both species are very similar from the immunological point of view, showing high correlations between both species when using cutaneous tests (R2=0.915) or specific IgE (R2=0.980). ImmunoCAP-inhibition and SDS-PAGE-IgE-Immunoblotting-inhibition probed with human sera, showed a total inhibition of specific IgE reactions by the heterologous antigens. CONCLUSION: The results obtained strongly suggest the great resemblance between the allergenic composition of both species.
Assuntos
Ácaros e Carrapatos/imunologia , Alérgenos/imunologia , Ácaros e Carrapatos/química , Ácaros e Carrapatos/classificação , Animais , Colômbia , Reações Cruzadas/imunologia , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Testes CutâneosRESUMO
En el presente estudio, se realiza una evaluacion de un nuevo metodo de ELISA, en el diagnostico inmunologico de la hidatidosis humana. Asimismo se comparan los resultados con los obtenidos con otras tecnicas, tales como aglutinacion del latex e inmunoelectroforesis, y se analizan segun la localizacion de los quistes. Por otra parte, se discuten las ventajas del metodo de ELISA, en relacion con la utilidad de su empleo en casos aislados de hidatidosis y estudios seroepidemiologicos
Assuntos
Equinococose , Técnicas ImunoenzimáticasAssuntos
Equinococose/diagnóstico , Feminino , Humanos , Imunodifusão , Testes de Fixação do Látex , MasculinoRESUMO
The immunoelectrophoretic reactivity of a "crude" and a "purified" antigens of Paracoccidiodes brasiliensis against the sera of confirmed cases of paracoccidioidomycosis and histoplasmosis was evaluated. The "crude" antigen revealed precipitating antibodies in both the homologous and the heterologous human sera; the number of precipitating systems being higher with the former. The "purified" antigen reacted only with the homologous sera. It is postulated that the use of such "purified" antigen may significantly reduce the non specificity of the serologic tests in the immunodiagnosis of paracoccidioidomycosis.
Assuntos
Reações Antígeno-Anticorpo , Blastomicose/imunologia , Fungos/imunologia , Histoplasmose/imunologia , Paracoccidioides/imunologia , Paracoccidioidomicose/imunologia , Antígenos de Fungos/análise , Epitopos , Humanos , Testes Sorológicos/métodosRESUMO
The results obtained by the immunoelectrophoresis (IEP) aand Casoni intradermal (IDR) tests in a group of 50 hydatidosis patients in which the presence of cysts was later confirmed at surgery, are analyzed. The sensitivity of the IEP test was 74%; the IDR test was positive in 54% of these patients and there was no correlation between the results of the two tests in 24% of the cases. The low sensitivity observed for the IDR test, its known nonspecificity and its poor correlation with IEP test results do not enable us to recommend its use for the diagnosis of hydatid disease or for the selection of cases in which an immunodiagnostic confirmation is feasible. This recommendation would be possible if appropriate antigens regarding specificity and reactivity were available, and could be employed with standardized methods to read and evaluate cutaneous reactivity.
Assuntos
Equinococose/imunologia , Estudos de Avaliação como Assunto , Humanos , Imunoeletroforese , Testes IntradérmicosRESUMO
The results obtained by the immunoelectrophoresis (IEP) aand Casoni intradermal (IDR) tests in a group of 50 hydatidosis patients in which the presence of cysts was later confirmed at surgery, are analyzed. The sensitivity of the IEP test was 74
; the IDR test was positive in 54
of these patients and there was no correlation between the results of the two tests in 24
of the cases. The low sensitivity observed for the IDR test, its known nonspecificity and its poor correlation with IEP test results do not enable us to recommend its use for the diagnosis of hydatid disease or for the selection of cases in which an immunodiagnostic confirmation is feasible. This recommendation would be possible if appropriate antigens regarding specificity and reactivity were available, and could be employed with standardized methods to read and evaluate cutaneous reactivity.
RESUMO
The results obtained by the immunoelectrophoresis (IEP) aand Casoni intradermal (IDR) tests in a group of 50 hydatidosis patients in which the presence of cysts was later confirmed at surgery, are analyzed. The sensitivity of the IEP test was 74
; the IDR test was positive in 54
of these patients and there was no correlation between the results of the two tests in 24
of the cases. The low sensitivity observed for the IDR test, its known nonspecificity and its poor correlation with IEP test results do not enable us to recommend its use for the diagnosis of hydatid disease or for the selection of cases in which an immunodiagnostic confirmation is feasible. This recommendation would be possible if appropriate antigens regarding specificity and reactivity were available, and could be employed with standardized methods to read and evaluate cutaneous reactivity.