RESUMO
Concern with antimicrobial resistance in animal production systems increases the interest toward integrative therapies. The objective of the present report was to report an integrative approach to a goat undergoing rumenotomy. A goat with ruminal acidosis underwent rumenotomy, corrective rumen suture, moxibustion, and treatment of the surgical wound (TFO) with sugar. In the first twenty days, acupuncture was performed on ST36, indirect moxibustion on Sp6 and CV8, and TFO with antibiotic ointment and sugar. The wound contracted 2cm, there was gradual return of ruminal dynamics, and moderate pain. Acupuncture continued from D21 to D50 on Yin tang, BL13, BL20, BL23, and BL22. Although there was drainage of ruminal fluid, the wound contracted 4cm during this period, pain was absent, and ruminal dynamics normal. During the last thirty days, the acupuncture technique surround the dragon was used and BL13 point needled, with complete closure of the wound. The TFO from D21 was performed solely with sugar. During treatment, there was modulation of the inflammatory response, with formation of granulation tissue and neovascularization. On D84, fibrinogen was 100mg/dL. The authors conclude that the use of sugar, acupuncture, and moxibustion contributed to return of normal ruminal motility, wound contraction, and complete tissue reepithelization.(AU)
A preocupação com a resistência antimicrobiana nos sistemas de produção animal aumenta o interesse pelas terapias integrativas. Objetiva-se relatar a abordagem integrativa ao paciente caprino submetido à ruminotomia. Uma cabra com acidose ruminal foi submetida à ruminotomia, ruminorrafia, moxabustão, acupuntura e tratamento da ferida operatória (TFO) com açúcar. Nos primeiros vinte dias realizou-se acupuntura em ST36, moxa indireta em Sp6 e CV8, TFO com pomada antibiótica e açúcar. A ferida contraiu 2cm, houve retorno gradual da dinâmica ruminal e dor moderada. A acupuntura continuou de D21 a D50 em Yin tang, BL13, BL20, BL23 e BL22. Apesar da ferida drenar fluido ruminal teve contração de 4cm nesse período, ausência de dor e retorno fisiológico da dinâmica ruminal. Nos últimos trinta dias foi realizada a técnica de acupuntura cercar o dragão e punturou-se o ponto BL13, com fechamento completo da ferida. O TFO a partir do D21 foi realizado somente com açúcar. Durante tratamento houve modulação da resposta inflamatória com formação de tecido de granulação e neoangiogênesse, o D84 revelou fibrinogênio 100mg/dL. Conclui-se que a utilização do açúcar, acupuntura e moxabustão contribuíram para o retorno fisiológico da motilidade ruminal, contração da ferida e completa reepitelização tecidual.(AU)
Assuntos
Animais , Rúmen/cirurgia , Acidose/veterinária , Ruminantes , Cabras , Ferida Cirúrgica/terapia , Rúmen/diagnóstico por imagem , Terapia por Acupuntura/veterinária , Medicina Tradicional Chinesa/métodos , Moxibustão/veterináriaRESUMO
Chondrosarcomas constitute the 3rd most common primary bone malignancy. These tumours grow slowly and rarely metastasize, usually having a good prognosis after surgery. Among patients registered and treated at the Brazilian National Institute of Traumatology and Orthopedics, an uncommon case of chondrosarcoma was identified in a 63-year-old man, who was diagnosed with multicentric chondrosarcoma of the appendicular skeleton. This example is atypical in the medical literature because multicentric tumours are different from metastatic events, and their frequency in chondrosarcoma is rare. This article therefore provides a rare case report alongside a review of additional cases in the medical literature.
Assuntos
Neoplasias Ósseas , Condrossarcoma , Neoplasias Ósseas/diagnóstico por imagem , Condrossarcoma/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , RadiografiaRESUMO
INTRODUCTION: The role of vitamin D on bone microarchitecture and fragility is not clear. OBJECTIVE: To investigate whether vitamin D deficiency (25(OH)D <20 ng/mL) increases cortical bone loss and the severity of fractures. DESIGN: Cross-sectional study of 287 elderly women with at least one prevalent low-impact fracture. METHODS: Biochemistry, X-rays to identify vertebral fractures (VFs) and to confirm non-vertebral fractures (NonVFs), and high-resolution peripheral quantitative computed tomography (HR-pQCT) to evaluate bone microstructure. RESULTS: Serum 25(OH)D levels were associated with body mass index (BMI: r = -0.161, P = 0.006), PTH (r = -0.165; P = 0.005), CTX (r = -0.119; P = 0.043) and vBMD at cortical bone (Dcomp: r = 0.132; P = 0.033) and entire bone (D100: r = 0.162 P = 0.009) at the distal radius, but not at the tibia. Age and PTH levels were potential confounding variables, but in the multiple linear regressions only BMI (95% CI: 0.11-4.16; P < 0.01), 25(OH)D (95% CI: -0.007 to 1.70; P = 0.05) and CTX (95% CI: -149.04 to 21.80; P < 0.01) predicted Dcomp, while BMI (95% CI: 1.13-4.18; P < 0.01) and 25(OH)D (95% CI: 0.24-1.52; P < 0.01) predicted D100. NonVFs predominated in patients with 25(OH)D <20 ng/mL (P = 0.013). Logistic regression analysis showed a decrease in the likelihood of presenting grade 2-3 VFs/NonVFs for every increase in 25(OH)D (OR = 0.962, 95% CI: 0.940-0.984; P = 0.001), BMI (OR = 0.932, 95% CI: 0.885-0.981; P = 0.007) and D100 at radius (OR = 0.994, 95% CI: 0.990-0.998; P = 0.005). CONCLUSION: In elderly patients with prevalent fractures, vitamin D deficiency was associated with cortical bone loss and severity of fractures.
Assuntos
Fraturas Ósseas/epidemiologia , Fraturas Ósseas/etiologia , Osteoporose/epidemiologia , Osteoporose/etiologia , Deficiência de Vitamina D/complicações , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Índice de Massa Corporal , Estudos Transversais , Feminino , Humanos , Fraturas por Osteoporose/epidemiologia , Fraturas por Osteoporose/etiologia , Hormônio Paratireóideo/sangue , Fraturas da Coluna Vertebral/epidemiologia , Fraturas da Coluna Vertebral/etiologiaRESUMO
Temporomandibular disorders (TMD) are associated with comorbidity. Shoulder pain is among the symptoms associated with TMD. The purpose of this study was to investigate the association between TMD and rotator cuff disease (RCD) and related genetic aspects. All subjects underwent orofacial and shoulder examinations. The control group comprised 30 subjects with no pain. Affected subjects were divided into three groups: RCD (TMD-free, n=16), TMD (RCD-free, n=13), and TMD/RCD (patients with both RCD and TMD, n=49). A total of eight single nucleotide polymorphisms in the ESRRB gene were investigated. A chemiluminescent immunoassay was used to measure estradiol levels. Surface electromyography recorded head and cervical muscle activity. The χ(2) test and Student t-test/Mann-Whitney test were used to assess the significance of nominal and continuous variables. A P-value of <0.05 was considered significant. TMD subjects were seven times more susceptible to RCD than controls. The rs1676303 TT (P=0.02) and rs6574293 GG (P=0.04) genotypes were associated with RCD and TMD, respectively. TMD/RCD subjects showed associations with rs4903399 (P=0.02), rs10132091 (P=0.02), and CTTCTTAG/CCTCTCAG (P=0.01) haplotypes and lower muscle activity. Estradiol levels were similar among groups. This study supports TMD as a risk factor for RCD. ESRRB haplotypes and low muscle activity are common biomechanical characteristics in subjects with both diseases.
Assuntos
Doenças Musculares/genética , Polimorfismo de Nucleotídeo Único , Receptores de Estrogênio/genética , Manguito Rotador , Transtornos da Articulação Temporomandibular/genética , Brasil , Comorbidade , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
The association among Anastrepha species, braconid parasitoids and host fruits in southern Bahia is recorded. Doryctobracon areolatus (Szépligeti) was associated with A. serpentina (Wied.) in Pouteria caimito, A. bahiensis Lima in Helicostylis tomentosa, A. sororcula Zucchi in Eugenia uniflora, and A. obliqua (Macquart) in Spondias purpurea. Anatrepha obliqua was unique in fruits of Averrhoa carambola, but associated with D. areolatus, Asobara anastrephae (Muesebeck) and Utetes anastrephae (Viereck). In Achras sapota, A. serpentina was associated with A. anastrephae and D. areolatus, while in Psidium guajava, A. fraterculus (Wied.) and A. sororcula were associated with D. areolatus and U. anastrephae.
Assuntos
Himenópteros/fisiologia , Plantas/parasitologia , Tephritidae/parasitologia , Animais , BrasilRESUMO
Devido à importância das moscas-das-frutas como pragas da fruticultura, este trabalho teve como objetivos caracterizar as populações das moscas-das-frutas em pomares comerciais de goiaba na região do Cariri, Estado do Ceará, por meio de índices faunísticos e estudar a flutuação populacional dessas moscas ao longo do ano. Para isso, foram instaladas armadilhas McPhail nos Municípios de Barbalha, Crato e Juazeiro do Norte. As armadilhas foram examinadas semanalmente e as moscas capturadas foram triadas, conservadas em álcool a 70% e identificadas. Constatou-se que Anastrepha zenildae (Wiedemann), A. sororcula Zucchi, A. fraterculus (Wiedemann), A. obliqua (Macquart) e Ceratitis capitata (Wiedemann) foram as moscas-das-frutas associadas à goiaba. Dessas, A. zenildae. A. sororcula e A. obliqua foram dominantes e constantes na região estudada, enquanto que A. fraterculus e C. capitata foram não-dominantes, mas também constantes. A. zenildae ocorreu em quase todos os meses do ano, exceto em outubro e dezembro de 2008, nos três pomares de goiaba do Cariri e com picos populacionais nos meses mais chuvosos e com temperaturas amenas.
Due to the importance of fruit-flies as pests in fruit growing, the present study was aimed to characterize the populations of fruit-flies in commercial guava orchards in the Cariri region (state of Ceará), Brazil, by means of faunistic indexes and to study their population fluctuation during the year. For this purpose, McPhail traps were installed in the counties Barbalha, Crato and Juazeiro do Norte. The traps were examined weekly and the captured flies were separated, preserved in 70% alcohol and identified. Anastrepha zenildae (Wiedemann), A. sororcula Zucchi, A. fraterculus (Wiedemann), A. obliqua (Macquart) and Ceratitis capitata (Wiedemann) were associated with guava fruits. Of them, A. zenildae. A. sororcula and A. obliqua were dominant and constant in the studied region, while A. fraterculus and C. capitata were non-dominant but constant. A. zenildae occurred in almost all months of the year, except October and December of 2008, with population peaks in the rainy season with its milder temperatures.
Assuntos
Animais , Tephritidae/anatomia & histologia , Tephritidae/classificação , Psidium , Brasil , Grupos de População AnimalRESUMO
ABSTRACT Due to the importance of fruit-flies as pests in fruit growing, the present study was aimed to characterize the populations of fruit-flies in commercial guava orchards in the Cariri region (state of Ceará), Brazil, by means of faunistic indexes and to study their population fluctuation during the year. For this purpose, McPhail traps were installed in the counties Barbalha, Crato and Juazeiro do Norte. The traps were examined weekly and the captured flies were separated, preserved in 70% alcohol and identified. Anastrepha zenildae (Wiedemann), A. sororcula Zucchi, A. fraterculus (Wiedemann), A. obliqua (Macquart) and Ceratitis capitata (Wiedemann) were associated with guava fruits. Of them, A. zenildae. A. sororcula and A. obliqua were dominant and constant in the studied region, while A. fraterculus and C. capitata were non-dominant but constant. A. zenildae occurred in almost all months of the year, except October and December of 2008, with population peaks in the rainy season with its milder temperatures.
RESUMO Devido à importância das moscas-das-frutas como pragas da fruticultura, este trabalho teve como objetivos caracterizar as populações das moscas-das-frutas em pomares comerciais de goiaba na região do Cariri, Estado do Ceará, por meio de índices faunísticos e estudar a flutuação populacional dessas moscas ao longo do ano. Para isso, foram instaladas armadilhas McPhail nos Municípios de Barbalha, Crato e Juazeiro do Norte. As armadilhas foram examinadas semanalmente e as moscas capturadas foram triadas, conservadas em álcool a 70% e identificadas. Constatou-se que Anastrepha zenildae (Wiedemann), A. sororcula Zucchi, A. fraterculus (Wiedemann), A. obliqua (Macquart) e Ceratitis capitata (Wiedemann) foram as moscas-das-frutas associadas à goiaba. Dessas, A. zenildae. A. sororcula e A. obliqua foram dominantes e constantes na região estudada, enquanto que A. fraterculus e C. capitata foram não-dominantes, mas também constantes. A. zenildae ocorreu em quase todos os meses do ano, exceto em outubro e dezembro de 2008, nos três pomares de goiaba do Cariri e com picos populacionais nos meses mais chuvosos e com temperaturas amenas.
RESUMO
Ticks are blood-feeding arthropods widely distributed in the world and vectors of several diseases. As haematophagy demands evasion strategies and repeatedly infested hosts develop protective immune responses, we investigated the mechanisms of the Rhipicephalus (Boophilus) microplus saliva anti-haemostatic activity and the possible relationship between the acquired natural anti-tick host resistance and anti-haemostatic action. For this purpose, we studied the effects of R. microplus saliva on different pathways of haemostasis and tested whether repeated infested bovine sera (RIBS) are able to abolish salivary anti-haemostatic activities. R. microplus saliva (i) displays inhibitory activity upon collagen-induced platelet aggregation; (ii) inhibits the induction of endothelial pro-coagulant state; and (iii) reduces thrombogenesis in vivo. RIBS were shown to be able to partially block the delay of coagulation and the anti-thrombotic effect of saliva, and to totally abolish the modulation of endothelium activation. Conversely, RIBS has no effect on the inhibition of platelet aggregation. These results show, for the first time, the neutralization ability of sera from acquired resistance hosts against tick anti-haemostatics. Moreover, this is the first report of a haematophagous parasite able to modulate endothelial cell pro-coagulant state, and addresses the presence of anti-platelet and anti-thrombotic activity in R. microplus saliva.
Assuntos
Doenças dos Bovinos/parasitologia , Hemostasia/efeitos dos fármacos , Soros Imunes , Rhipicephalus/metabolismo , Saliva/fisiologia , Infestações por Carrapato/parasitologia , Animais , Coagulação Sanguínea/efeitos dos fármacos , Bovinos , Doenças dos Bovinos/imunologia , Linhagem Celular , Modelos Animais de Doenças , Células Endoteliais , Hemostasia/fisiologia , Interações Hospedeiro-Parasita , Humanos , Soros Imunes/efeitos dos fármacos , Soros Imunes/imunologia , Soros Imunes/farmacologia , Masculino , Testes de Neutralização , Agregação Plaquetária/efeitos dos fármacos , Coelhos , Ratos , Ratos Wistar , Rhipicephalus/fisiologia , Saliva/imunologia , Infestações por Carrapato/imunologia , Trombose VenosaRESUMO
This work was carried out in orchards of guava progenies, and loquat and peach cultivars, in Monte Alegre do Sul, SP, Brazil, in 2002 and 2003. Guavas and loquats were bagged and unbagged bi-weekly and weekly, respectively, for assessment of the infestation period. Peach was only bagged weekly. The assays started when the fruits were at the beginning of development, but still green. Ripe fruits were taken to the laboratory and placed individually into plastic cups. McPhail plastic traps containing torula yeast were hung from January 2002 to January 2004 to assess the fruit fly population in each orchard, but only the Ceratitis capitata population is here discussed. Five tephritid species were reared from the fruits: Anastrepha bistrigata Bezzi, A. fraterculus (Wiedemann), A. obliqua (Macquart), A. sororcula Zucchi, and C. capitata, in addition to six lonchaeid species: Neosilba certa (Walker), N. glaberrima (Wiedemann), N. pendula (Bezzi), N. zadolicha McAlpine and Steyskal, Neosilba sp. 4, and Neosilba sp. 10 (both species are in the process of being described by P. C. Strikis), as well as some unidentified Neosilba species. Ten parasitoid species were obtained from fruit fly puparia, of which five were braconids: Asobara anastrephae (Muesebeck), Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan, and Utetes anastrephae (Viereck), and five figitids: Aganaspis pelleranoi (Brèthes), Dicerataspis grenadensis Ashmead, Lopheucoila anastrephae (Rhower), Leptopilina boulardi (Barbotin, Carlton and Kelner-Pillaut), and Trybliographa infuscata Diaz, Gallardo and Uchôa. Ceratitis capitata showed a seasonal behavior with population density peaking at the second semester of each year. Anastrepha and Neosilba species remained in the orchards throughout both years.
Assuntos
Eriobotrya/parasitologia , Himenópteros/fisiologia , Prunus/parasitologia , Psidium/parasitologia , Tephritidae/fisiologia , Animais , Biodiversidade , Brasil , Interações Hospedeiro-Parasita , Densidade Demográfica , Dinâmica Populacional , Estações do Ano , Tephritidae/parasitologiaRESUMO
This work was carried out in orchards of guava progenies, and loquat and peach cultivars, in Monte Alegre do Sul, SP, Brazil, in 2002 and 2003. Guavas and loquats were bagged and unbagged bi-weekly and weekly, respectively, for assessment of the infestation period. Peach was only bagged weekly. The assays started when the fruits were at the beginning of development, but still green. Ripe fruits were taken to the laboratory and placed individually into plastic cups. McPhail plastic traps containing torula yeast were hung from January 2002 to January 2004 to assess the fruit fly population in each orchard, but only the Ceratitis capitata population is here discussed. Five tephritid species were reared from the fruits: Anastrepha bistrigata Bezzi, A. fraterculus (Wiedemann), A. obliqua (Macquart), A. sororcula Zucchi, and C. capitata, in addition to six lonchaeid species: Neosilba certa (Walker), N. glaberrima (Wiedemann), N. pendula (Bezzi), N. zadolicha McAlpine and Steyskal, Neosilba sp. 4, and Neosilba sp. 10 (both species are in the process of being described by P. C. Strikis), as well as some unidentified Neosilba species. Ten parasitoid species were obtained from fruit fly puparia, of which five were braconids: Asobara anastrephae (Muesebeck), Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan, and Utetes anastrephae (Viereck), and five figitids: Aganaspis pelleranoi (Brèthes), Dicerataspis grenadensis Ashmead, Lopheucoila anastrephae (Rhower), Leptopilina boulardi (Barbotin, Carlton and Kelner-Pillaut), and Trybliographa infuscata Diaz, Gallardo and Uchôa. Ceratitis capitata showed a seasonal behavior with population density peaking at the second semester of each year. Anastrepha and Neosilba species remained in the orchards throughout both years.
Este trabalho foi realizado em três pomares em Monte Alegre do Sul, SP, em 2002 e 2003, representados por coleção de progênies de goiabeiras, de cultivares de nespereiras e de cultivares de pessegueiros. O período de infestação foi determinado por meio de ensacamento e desensacamento quinzenal e semanal de goiabas e nêsperas, respectivamente, e pelo ensacamento semanal de pêssegos. Os ensaios iniciaram-se com os frutos verdes (princípio de desenvolvimento). Os frutos maduros foram levados ao laboratório e acondicionados individualmente em copos plásticos. A flutuação populacional de Ceratitis capitata (Wiedemann) foi avaliada por meio de armadilhas plásticas modelo McPhail com torula em cada pomar, de janeiro/2002 a janeiro/2004. Dos frutos foram obtidas cinco espécies de tefritídeos: Anastrepha bistrigata Bezzi, A. fraterculus (Wiedemann), A. obliqua (Macquart), A. sororcula Zucchi e C. capitata e seis de lonqueídeos: Neosilba certa (Walker), N. glaberrima (Wiedemann), N. pendula (Bezzi), N. zadolicha McAlpine and Steyskal, Neosilba sp. 4 e Neosilba sp. 10, além de algumas espécies não-identificadas. Foram obtidas 10 espécies de parasitóides, cinco da família Braconidae - Asobara anastrephae (Muesebeck), Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan e Utetes anastrephae (Viereck) - e cinco da família Figitidae - Aganaspis pelleranoi (Brèthes), Dicerataspis grenadensis Ashmead, Lopheucoila anastrephae (Rhower), Leptopilina boulardi (Barbotin, Carlton and Kelner-Pillaut) e Trybliographa infuscata Diaz, Gallardo and Uchôa. Ceratitis capitata apresentou comportamento sazonal com picos populacionais durante o segundo semestre dos dois anos. As espécies de Anastrepha e de Neosilba permaneceram nos pomares durante os dois anos.
Assuntos
Animais , Eriobotrya/parasitologia , Himenópteros/fisiologia , Prunus/parasitologia , Psidium/parasitologia , Tephritidae/fisiologia , Biodiversidade , Brasil , Interações Hospedeiro-Parasita , Densidade Demográfica , Dinâmica Populacional , Estações do Ano , Tephritidae/parasitologiaRESUMO
The tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite that causes considerable economic losses to cattle breeding. Although R. microplus saliva contains several molecules that interfere with the blood coagulation process, so far the systemic alterations in the host hemostatic system have not been described. This study aims to determine if R. microplus infestation induces any disturbance to the host's hemostatic system. To address these questions, six calves were experimentally infested with 20,000 R. microplus larvae and their blood was collected before and 7, 14 and 21 days post-infestation. Collagen and ADP-induced platelet aggregation as well as coagulation (activated partial thromboplastin time and prothrombin time) decreased in infested bovines. Platelet blood count and fibrinogen increased during the course of infestation, probably as a compensatory response. These alterations may play a role in host health status, and show that the host cannot fully counteract the tick anti-hemostatic mechanisms.
Assuntos
Doenças dos Bovinos/sangue , Doenças dos Bovinos/parasitologia , Hemostasia/fisiologia , Rhipicephalus/crescimento & desenvolvimento , Infestações por Carrapato/sangue , Infestações por Carrapato/veterinária , Animais , Bovinos , Feminino , Fibrinogênio/análise , Tempo de Tromboplastina Parcial/veterinária , Agregação Plaquetária/fisiologia , Contagem de Plaquetas/veterinária , Tempo de Protrombina/veterinária , Infestações por Carrapato/parasitologiaRESUMO
ABSTRACT The objective of this study was to assess the diversity of fruit-fly species (Diptera: Tephritidae) in the Baixo Jaguaribe region, state of Ceará, Brazil. The collections of the fruit flies were made from October/2002 to December/2003, with McPhail traps and 5% hydrolyzed corn protein (food bait). The traps were set up in host trees of the fruit flies: guava Psidium guajava, mango Mangiferaindica, red-coat plum Spondias purpurea, West Indian cherry Malpighiaemarginata, star fruit Avherroa carambola, papaya Caricapapaya and melon Cucumis melo. In the laboratory, the fruit flies captured were separated from other insects, counted and preserved in 70% alcohol, and then identified by species. The species collected were: Anastrepha zenildae Zucchi (74.59%), Anastrepha sororcula Zucchi (13.87%), Anastrepha obliqua (Macquart) (1.08%), Anastrepha daciformis Bezzi (0.09%), Anastrepha consobrina (Loew) (0.09%), Anastrepha pickeli Lima (0.09%) and Ceratitis capitata (Wied.) (10.19%).
RESUMO O objetivo deste estudo foi conhecer a diversidade de espécies de moscas-das-frutas (Diptera: Tephritidae) na região do Baixo Jaguaribe. As coletas das moscas-das-frutas foram realizadas de outubro/2002 a dezembro/2003, utilizando-se armadilhas McPhail e proteína hidrolisada de milho a 5% (atrativo alimentar). As armadilhas foram instaladas em frutíferas potencialmente hospedeiras de moscas-das-frutas: goiaba Psidium guajava, manga Mangifera indica, serigüela Spondias purpurea, acerola Malpighiaemarginata, carambola Avherroacarambola, mamão Carica papaya e melão Cucumis melo. No laboratório, as moscas-das-frutas foram separadas dos outros insetos capturados, contadas e acondicionadas em recipientes com álcool 70%, até a identificação específica. As espécies coletadas foram: Anastrepha zenildae Zucchi (74,59%), Anastrepha sororcula Zucchi (13,87%), Anastrepha obliqua (Macquart) (1,08%), Anastrepha daciformis Bezzi (0,09%),Anastrepha consobrina (Loew) (0,09%), Anastrepha pickeli Lima (0,09%) e Ceratitis capitata (Wied.) (10,19%).
RESUMO
ABSTRACT This paper presents a one-year survey of the fruit flies species and their host plants in the Didactic Vegetable Garden of the University of Ceará (CCA/UFC) in Fortaleza, Ceará State, Brazil. Fruit samples were collected biweekly from January 2005 until January 2006 at different heights within the canopies of the host trees and from fruits recently fallen to the ground. Fruit samples were kept individually in sealed plastic containers in order to establish the precise association between the host fruits and fruit-fly species. Two species,Anastrepha. Anastrephazenildae Zucchi andAnastrepha sorocula Zucchi were obtained. These species were exclusively associated with guava (Psidium guajava L.) fruits. Another three male specimens ofAnastrepha were obtained from ciruela (Spondias purpurea L.) fruits. Specimens ofCeratitis capitata (Wied.) were found to be associated with nine host plants: P. guajava., mango Mangifera indica L.,S. purpurea, Java plum Syzygiumcumini(L.), Malabar plum Syzygium jambos (L.), Surinam cherry Eugenia uniflora L., star fruit Averrhoa carambola L., tangerine Citrus reticulata Blanco and Barbados cherry Malpighia glabra L. From January through March, 2005, the highest rate of C. capitata was obtained from guava fruits.
RESUMO Este trabalho apresenta os resultados obtidos no levantamento das plantas hospedeiras de moscas-das-frutas no pomar da Horta Didática no Campus do Pici da Universidade Federal do Ceará (UFC), em Fortaleza, Ceará. Foram realizadas coletas quinzenais de frutos com sintomas de ataque de moscas-das-frutas em diversas alturas da copa das fruteiras e os caídos no solo no período de janeiro de 2005 a janeiro de 2006. Cada amostra de frutos foi mantida isoladamente em bandejas plásticas a fim de determinar a associação precisa entre a planta hospedeira e as espécies de moscas-das-frutas. Foram identificadas duas espécies de Anastrepha. A. zenildae Zucchi e A. sororcula Zucchi, infestando frutos de goiaba (Psidium guajava L.). Três exemplares machos de Anastrepha spp. foram obtidos de frutos de cirigüela (Spondias purpurea L.). Foram obtidos exemplares de Ceratitis capitata (Wied. 1824) em nove espécies de plantas: goiaba (P. guajava), manga (Mangifera indica L.), cirigüela (S. purpurea), azeitona preta (Syzygium cumini L.), jambo (Syzygium jambos L. Alston), pitanga (Eugenia unifloraL.), carambola (Averrhoa carambola L.), tangerina (Citrus reticulata Blanco) e acerola (Malpighia glabra L.). Cerca de 72% dos exemplares de C. capitata foram obtidos de goiaba ao longo do período da amostragem, com maiores índices de infestação em janeiro e março de 2005.
RESUMO
Lonomia obliqua envenomation induces an intense burning sensation at the site of contact and severe hemorrhage followed by edema and hypotension, and after few days death can occur usually due to acute renal failure. In order to understand more about the envenomation syndrome, the present study investigates the role played by kallikrein-kinin system (KKS) in edematogenic and hypotensive responses to the envenomation by L. obliqua. The incubation of L. obliqua caterpillar bristles extract (LOCBE) with plasma results in kallikrein activation, measured by cromogenic assay using the kallikrein synthetic substrate S-2302 (H-D-Pro-Phe-Arg-pNA). It was also showed that LOCBE was able to release kinins from low-molecular weight kininogen (LMWK). Moreover, it was demonstrated that previous administration of a kallikrein inhibitor (aprotinin) or bradykinin B2 receptor antagonist (HOE-140) significantly reduces the edema and hypotension in response to LOCBE, using mouse paw edema bioassay and mean arterial blood pressure analysis, respectively. The results demonstrate a direct involvement of the KKS in the edema formation and in the fall of arterial pressure that occur in the L. obliqua envenomation syndrome.
Assuntos
Venenos de Artrópodes/toxicidade , Edema/induzido quimicamente , Hipotensão/induzido quimicamente , Mordeduras e Picadas de Insetos/metabolismo , Sistema Calicreína-Cinina/efeitos dos fármacos , Mariposas/química , Análise de Variância , Animais , Venenos de Artrópodes/análise , Pressão Sanguínea/efeitos dos fármacos , Feminino , Cobaias , Cininogênios , Larva/química , Masculino , Camundongos , Oligopeptídeos , Ratos , Ratos WistarRESUMO
Brazilian scientific output exhibited a 4-fold increase in the last two decades because of the stability of the investment in research and development activities and of changes in the policies of the main funding agencies. Most of this production is concentrated in public universities and research institutes located in the richest part of the country. Among all areas of knowledge, the most productive are Health and Biological Sciences. During the 1998-2002 period these areas presented heterogeneous growth ranging from 4.5% (Pharmacology) to 191% (Psychiatry), with a median growth rate of 47.2%. In order to identify and rank the 20 most prolific institutions in these areas, searches were made in three databases (DataCAPES, ISI and MEDLINE) which permitted the identification of 109,507 original articles produced by the 592 Graduate Programs in Health and Biological Sciences offered by 118 public universities and research institutes. The 20 most productive centers, ranked according to the total number of ISI-indexed articles published during the 1998-2003 period, produced 78.7% of the papers in these areas and are strongly concentrated in the Southern part of the country, mainly in São Paulo State.
Assuntos
Bibliometria , Biologia/estatística & dados numéricos , Pesquisa/estatística & dados numéricos , Pesquisa Biomédica/economia , Pesquisa Biomédica/estatística & dados numéricos , Brasil , Bases de Dados Bibliográficas/estatística & dados numéricos , Humanos , Pesquisa/economia , Apoio à Pesquisa como Assunto/estatística & dados numéricos , Universidades/estatística & dados numéricosRESUMO
Brazilian scientific output exhibited a 4-fold increase in the last two decades because of the stability of the investment in research and development activities and of changes in the policies of the main funding agencies. Most of this production is concentrated in public universities and research institutes located in the richest part of the country. Among all areas of knowledge, the most productive are Health and Biological Sciences. During the 1998-2002 period these areas presented heterogeneous growth ranging from 4.5 percent (Pharmacology) to 191 percent (Psychiatry), with a median growth rate of 47.2 percent. In order to identify and rank the 20 most prolific institutions in these areas, searches were made in three databases (DataCAPES, ISI and MEDLINE) which permitted the identification of 109,507 original articles produced by the 592 Graduate Programs in Health and Biological Sciences offered by 118 public universities and research institutes. The 20 most productive centers, ranked according to the total number of ISI-indexed articles published during the 1998-2003 period, produced 78.7 percent of the papers in these areas and are strongly concentrated in the Southern part of the country, mainly in São Paulo State.
Assuntos
Humanos , Bibliometria , Biologia/estatística & dados numéricos , Pesquisa/normas , Universidades/normas , Brasil , Pesquisa Biomédica/economia , Pesquisa Biomédica/estatística & dados numéricos , Bases de Dados Bibliográficas/estatística & dados numéricos , Apoio à Pesquisa como Assunto , Pesquisa/economiaRESUMO
OBJECTIVE: To measure the activity of serum phosphodiesterase (PDE) in pre-eclampsia. DESIGN: Case-control study. SETTING: Nephrology Laboratory and Obstetrics Unit at São Lucas Hospital from Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil. SAMPLE: Twenty-nine normal and 28 pregnant women with pre-eclampsia. METHODS: Serum was collected from women with pre-eclampsia, at the time of diagnosis, and from gestation-matched controls. Circulating PDE activity was assessed by measuring consumption of the substrate thymidine 5'-monophosphate p-nitrophenyl ester and expressed as PDE units/l. MAIN OUTCOME MEASURES: Serum PDE Activity. RESULTS: Mean substrate consumption was higher in pre-eclamptic condition (V(max)= 15.8 +/- 1.4 versus 12.7 +/- 0.9 U/L, P= 3.7 x 10(-14)). CONCLUSION: These data suggest that altered PDE activity may play a role in pre-eclampsia endothelial dysfunction.
Assuntos
Diester Fosfórico Hidrolases/metabolismo , Pré-Eclâmpsia/enzimologia , Adulto , Estudos de Casos e Controles , Feminino , Humanos , GravidezRESUMO
RESUMO Tetrastichus giffardianus (Hymenoptera: Eulophidae) foi introduzido em 1937 no Estado de São Paulo, Brasil, para o controle biológico da mosca do mediterrâneo, Ceratitis capitata (Diptera: Tephritidae). Apesar de na época este parasitóide ter sido criado em grandes quantidades e liberado em várias fazendas, nunca mais foi relatado no país, levantando controvérsias sobre seu estabelecimento. Este trabalho relata a redescoberta desta espécie no Brasil, mas em locais distantes de onde fora originalmente liberado. Estas novas ocorrências estão na Região Nordeste, onde T. giffardianus parece ter se adaptado melhor do que no território paulista.
ABSTRACT Tetrastichus giffardianus (Hymenoptera: Eulophidae) was introduced in 1937 in São Paulo state, southeast Brazil, to control the medfly, Ceratitis capitata (Diptera: Tephritidae). In spite of its being mass reared and released in several farms at that time, there were no further reports about its occurrence in the country, giving rise to controversies about its establishment. This paper deals with the rediscovering of T. giffardianus, but in a region far from where it was released. The new findings are in northeast Brazil, where this species seems to be better adapted to the local conditions.
RESUMO
Canatoxin is a toxic protein from Canavalia ensiformis seeds, lethal to mice (LD(50)=2 mg/kg) and insects. Further characterization of canatoxin showed that its main native form (184 kDa) is a non-covalently linked dimer of a 95 kDa polypeptide containing zinc and nickel. Partial sequencing of internal peptides indicated homology with urease (EC 3.5.1.5) from the same seed. Canatoxin has approx. 30% of urease's activity for urea, and K(m) of 2-7 mM. The proteins differ in their affinities for metal ions and were separated by affinity chromatography on a Zn(2+) matrix. Similar to canatoxin, urease activates blood platelets and interacts with glycoconjugates. In contrast with canatoxin, no lethality was seen in mice injected with urease (10 mg/kg). Pretreatment with p-hydroxymercuribenzoate irreversibly abolished the ureolytic activity of both proteins. On the other hand, p-hydroxymercuribenzoate-treated canatoxin was still lethal to mice, and both treated proteins were fully active in promoting platelet aggregation and binding to glycoconjugates. Taken together, our data indicate that canatoxin is a variant form of urease. Moreover, we show for the first time that these proteins display several biological effects that are unrelated to their enzymic activity for urea.
Assuntos
Lectinas/química , Lectinas/metabolismo , Phaseolus/enzimologia , Proteínas de Plantas , Toxinas Biológicas , Urease/química , Urease/fisiologia , Sequência de Aminoácidos , Animais , Plaquetas/enzimologia , Cromatografia em Gel , Dimerização , Relação Dose-Resposta a Droga , Eletroforese em Gel de Poliacrilamida , Hemaglutininas/metabolismo , Hidroximercuribenzoatos/farmacologia , Cinética , Camundongos , Dados de Sequência Molecular , Peso Molecular , Lectinas de Plantas , Ligação Proteica , Coelhos , Homologia de Sequência de Aminoácidos , Ureia/metabolismo , Urease/metabolismo , Zinco/metabolismoRESUMO
The number of accidents caused by injection of the venom of Lonomia obliqua caterpillars in Southern Brazil has increased in the last years. Even though this kind of envenomation has an important social and medical impact, nothing is known about the cellular structures responsible for the production and secretion of this venom. Here we identify and analyse morphological structures possibly responsible for the production and secretion of the active principles of the venom, as well as the histological relationship of these structures with the urticating spines of L. obliqua. Detailed microscopic observations showed that: (a) L. obliqua has a complex tegument, with several cuticular specializations, (b) there are no pores along the tegument neither in the spines and (c) the spines bear a hollow canal--where the venom is deposited--and an area that can be easily broken when touched, releasing the venom. Histological and histochemical techniques revealed that: (a) there is no single gland cell that produces the venom, (b) a secretory epithelium, composed of cells containing vesicles that increase in size and number as they reach the apical region, underlies the tegument and the spines and is responsible for secretion of the venomous substances and (c) the venom is deposited in the subcuticular space and at the tips of the spines.