RESUMO
The authors show that the common fixative fluids currently used for the fixation of tissues for histological studies gave variable results when used with fresh water fish. Good results were obtained with the following mixture; formalin, 100 ml; mercuric chloride, 1,0 g; glacial acetic acid, 0,5 ml.
As experiências realizadas demonstraram que a preservação dos tecidos de peixes de água doce varia com a natureza do material e do fixador. Tratando diferentes tecidos de Pimelodus maculatus com variados líquidos fixadores, os autores observaram melhores resultados com a seguinte mistura: formol, 10% 100 ml, bicloreto de mercúrio, 1,0 g, ácido acético glacial, 0,5 ml.
RESUMO
Analysis were carried out on the intermediate section of Gallus gallus thoracic aorta during development in chickens ranging from 1 day to 3 years, divided in 12 age groups. Classic techniques of wax inclusion and staining were employed for the connective and muscle tissues. The reticular fibers were also studied after they had been sectioned in the freeze microtome through silver impregnation. The authors observed that the vessel undergoes structural variations within the considered age intervals in the architectonical disposition and morphological characteristics of its constituent elements.
Análises foram levadas a efeito na secção intermediária da aorta torácica de Gallus gallus, durante o seu desenvolvimento em animais de 1 dia a 3 anos, divididos em 12 grupos segundo a idade. Foram empregadas técnicas clássicas de inclusão e coloração para os tecidos conjuntivo e muscular. As fibras reticulares foram também estudadas depois de seccionadas em micrótomo de congelação após impregnação com prata. Os autores observaram que os vasos sofrem variações estruturais dentro dos limites de idade considerados na disposição arquitetônica e características morfológicas dos seus elementos constitutivos.
RESUMO
The histology of the medium third of the thoracic aorta has been studied in the following species: Equus caballus, Sus scrofa, Canis familiaris, Felis domesticus, Bos indicus and Capra hircus. The observations permit the conclusion that the different components of the aortic wall behave in a variable form, according to its species. In all species studied, it has been verified that the basilar membrane is always discontinuous and that the subendothelial layer has very variable thickness. While the intern elastic membrane has only been observed in Sus scrofa, Felis domesticus and Capra hircus, a real extern elastic membrane was found in none of the mamiferous studied.
Foi estudada a histologia do terço médio da aorta torácica das seguintes espécies: Equus caballus, Sus scrofa, Canis familiaris, Felis domesticus, Bos indicus e Capra hircus. As observações permitem afirmar que os distintos componentes da parede aórtica comportam-se de modo variável, de acordo com a espécie. Em tôdas as espécies estudadas, verificou-se que a membrana basilar é sempre descontínua e que a camada subendotelial é de espessura variável. Enquanto a membrana elástica interna somente foi observada em Sus scrofa, Felis domesticus e Capra hircus, em nenhum dos mamíferos estudados uma verdadeira membrana elástica externa foi encontrada.
RESUMO
The histology of the medium third of the thoracic aorta has been studied in the following species: Equus caballus, Sus scrofa, Canis familiaris, Felis domesticus, Bos indicus and Capra hircus. The observations permit the conclusion that the different components of the aortic wall behave in a variable form, according to its species. In all species studied, it has been verified that the basilar membrane is always discontinuous and that the subendothelial layer has very variable thickness. While the intern elastic membrane has only been observed in Sus scrofa, Felis domesticus and Capra hircus, a real extern elastic membrane was found in none of the mamiferous studied.
Foi estudada a histologia do terço médio da aorta torácica das seguintes espécies: Equus caballus, Sus scrofa, Canis familiaris, Felis domesticus, Bos indicus e Capra hircus. As observações permitem afirmar que os distintos componentes da parede aórtica comportam-se de modo variável, de acordo com a espécie. Em tôdas as espécies estudadas, verificou-se que a membrana basilar é sempre descontínua e que a camada subendotelial é de espessura variável. Enquanto a membrana elástica interna somente foi observada em Sus scrofa, Felis domesticus e Capra hircus, em nenhum dos mamíferos estudados uma verdadeira membrana elástica externa foi encontrada.
RESUMO
Analysis were carried out on the intermediate section of Gallus gallus thoracic aorta during development in chickens ranging from 1 day to 3 years, divided in 12 age groups. Classic techniques of wax inclusion and staining were employed for the connective and muscle tissues. The reticular fibers were also studied after they had been sectioned in the freeze microtome through silver impregnation. The authors observed that the vessel undergoes structural variations within the considered age intervals in the architectonical disposition and morphological characteristics of its constituent elements.
Análises foram levadas a efeito na secção intermediária da aorta torácica de Gallus gallus, durante o seu desenvolvimento em animais de 1 dia a 3 anos, divididos em 12 grupos segundo a idade. Foram empregadas técnicas clássicas de inclusão e coloração para os tecidos conjuntivo e muscular. As fibras reticulares foram também estudadas depois de seccionadas em micrótomo de congelação após impregnação com prata. Os autores observaram que os vasos sofrem variações estruturais dentro dos limites de idade considerados na disposição arquitetônica e características morfológicas dos seus elementos constitutivos.
RESUMO
The authors show that the common fixative fluids currently used for the fixation of tissues for histological studies gave variable results when used with fresh water fish. Good results were obtained with the following mixture; formalin, 100 ml; mercuric chloride, 1,0 g; glacial acetic acid, 0,5 ml.
As experiências realizadas demonstraram que a preservação dos tecidos de peixes de água doce varia com a natureza do material e do fixador. Tratando diferentes tecidos de Pimelodus maculatus com variados líquidos fixadores, os autores observaram melhores resultados com a seguinte mistura: formol, 10% 100 ml, bicloreto de mercúrio, 1,0 g, ácido acético glacial, 0,5 ml.
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O artigo não apresenta resumo.
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O artigo não apresenta resumo.
RESUMO
It is studied in the present work, the pathological-anatomy of the spontaneus and experimental coccidioidomycosis in dogs. It was studied in 5 cases with natural illness observed in Guatemala and 10 in experimental conditions, of which 7 were inoculated in the pleural cavity with suspention of artrospora of Coccidioides immitis in physiologyc solution and 3 were put to live for 6 months with the ones experimentaly inoculated. Of the last ones, one was infected. In this work it is described the macro and microscopic lesions of the coccidioidomycosis in natural and experimental conditions. It is discussed the relative aspects of the lesions, the difficulty of diagnosis in the spontaneus cases, ways of infection and dissemination of the spore in the experimental cases, and also the ethiopathogenesis of the glomerulonefritis which are observed in the dogs with coccidioidomycosis, admiting a mechanism of hypersensibility. It is assumed that the cells of the pleural-mesotelium can present phagocytic prosperties, a thing that has to be confirmed in future investigations. Also there is a suggestion that these cells take the spore to the pulmonar tissue whereby the infection disseminates itself through the lympho-hematogenic vessels. It is confirmed that the respiratory system is the principal way of this mycosis though the skin can also be the infection way of the disease. Finally the
O artigo apresenta resumo em inglês.
RESUMO
It is studied in the present work, the pathological-anatomy of the spontaneus and experimental coccidioidomycosis in dogs. It was studied in 5 cases with natural illness observed in Guatemala and 10 in experimental conditions, of which 7 were inoculated in the pleural cavity with suspention of artrospora of Coccidioides immitis in physiologyc solution and 3 were put to live for 6 months with the ones experimentaly inoculated. Of the last ones, one was infected. In this work it is described the macro and microscopic lesions of the coccidioidomycosis in natural and experimental conditions. It is discussed the relative aspects of the lesions, the difficulty of diagnosis in the spontaneus cases, ways of infection and dissemination of the spore in the experimental cases, and also the ethiopathogenesis of the glomerulonefritis which are observed in the dogs with coccidioidomycosis, admiting a mechanism of hypersensibility. It is assumed that the cells of the pleural-mesotelium can present phagocytic prosperties, a thing that has to be confirmed in future investigations. Also there is a suggestion that these cells take the spore to the pulmonar tissue whereby the infection disseminates itself through the lympho-hematogenic vessels. It is confirmed that the respiratory system is the principal way of this mycosis though the skin can also be the infection way of the disease. Finally the
O artigo apresenta resumo em inglês.
RESUMO
The authors studied the morphological modifications of the vagina in "Bos indicus during the sexual cycle, on 18 animals, sacrified in different periods after clinical observation. Anatomical and histological pictures are described and the results are analised based on the literature, which allows the following conclusions: I Based on material examined it seems that there are no differences between morphological modifications in vagina during estral cicle of the indian and european bovines. II In the immatured animals, we found a morphological variation on the vagina, probably related to hormonal ovary activity. III Indian as european bovines present correlation between histological modifications on the vagina and ovary. IV Histological pictures observed in the vagina suggest that there is true mucigenic tubular glands and mucosae folds. V It was variable plasmocitic infiltration in the vaginal mucosae, probably in dependence of cyclic modifications.
O artigo apresenta resumo em inglês.
RESUMO
The authors studied the morphological modifications of the vagina in "Bos indicus during the sexual cycle, on 18 animals, sacrified in different periods after clinical observation. Anatomical and histological pictures are described and the results are analised based on the literature, which allows the following conclusions: I Based on material examined it seems that there are no differences between morphological modifications in vagina during estral cicle of the indian and european bovines. II In the immatured animals, we found a morphological variation on the vagina, probably related to hormonal ovary activity. III Indian as european bovines present correlation between histological modifications on the vagina and ovary. IV Histological pictures observed in the vagina suggest that there is true mucigenic tubular glands and mucosae folds. V It was variable plasmocitic infiltration in the vaginal mucosae, probably in dependence of cyclic modifications.
O artigo apresenta resumo em inglês.
RESUMO
Observations about the anatomic and histophysiologic modifications o f the ovary in 18 Brahma cattle animals, have been carried out. Five of these animals were between 9 to 14 months old, that is, not in puberty age; six were between 22 to 31 months old, but had never calved, and seven were between 60 to 120 months old and had calved several times. All the adult animals were sacrificed during a distinct phase of the sexual cycle. In this work, almost every modification observed agreed with that ones already described in the European cattle. As a matter of fact the authors have find the following: 1) Graafian follicles with hyperplasia of the theca interna. This fact could, probably be related with the secretion of progesterona by those cellular elements; 2) That the eosinophiles invade the cells of both layers of the theca, as also the granulosa cells, when the corpus luteum begins its development; 3) As already have been described in other species of animals, hyperplasia and hyperthophy of both thecal and granulosa cells is observed, still during the time that the corpus luteum starts its development.
O artigo apresenta resumo em inglês.
RESUMO
After revision of the literature, the authors present a study about mast cell fixation in several times of domestics and laboratories animals. It was experimented several fixatives habitually used and some mixture with lead subacetate in alcoholic solution. The authors obtained good results in freezer sections with the following mixture: Alcohol 50" ................................... 100,0 ml Lead subacetate ........................... 1,0 g Acetic acid ................................... 1,0 ml
O artigo apresenta resumo em inglês.
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The authors describe the anatomic-pathological and haematological features of a multiple mastocitoma in a dog. They draw the attention of pathologists to this type of blastoma which has not yet been reported in Brazil, although being very frequent in others countries. They try experimental reproduction, but without success. After discussing the fixation as well as the histamine and heparin contents of the neoplastic cells, the authors coment on the local and haematic eosinophilie suggesting an explanation for this fact.
O artigo apresenta resumo em inglês.
RESUMO
The authors describe the anatomic-pathological and haematological features of a multiple mastocitoma in a dog. They draw the attention of pathologists to this type of blastoma which has not yet been reported in Brazil, although being very frequent in others countries. They try experimental reproduction, but without success. After discussing the fixation as well as the histamine and heparin contents of the neoplastic cells, the authors coment on the local and haematic eosinophilie suggesting an explanation for this fact.
O artigo apresenta resumo em inglês.
RESUMO
After revision of the literature, the authors present a study about mast cell fixation in several times of domestics and laboratories animals. It was experimented several fixatives habitually used and some mixture with lead subacetate in alcoholic solution. The authors obtained good results in freezer sections with the following mixture: Alcohol 50" ................................... 100,0 ml Lead subacetate ........................... 1,0 g Acetic acid ................................... 1,0 ml
O artigo apresenta resumo em inglês.
RESUMO
Observations about the anatomic and histophysiologic modifications o f the ovary in 18 Brahma cattle animals, have been carried out. Five of these animals were between 9 to 14 months old, that is, not in puberty age; six were between 22 to 31 months old, but had never calved, and seven were between 60 to 120 months old and had calved several times. All the adult animals were sacrificed during a distinct phase of the sexual cycle. In this work, almost every modification observed agreed with that ones already described in the European cattle. As a matter of fact the authors have find the following: 1) Graafian follicles with hyperplasia of the theca interna. This fact could, probably be related with the secretion of progesterona by those cellular elements; 2) That the eosinophiles invade the cells of both layers of the theca, as also the granulosa cells, when the corpus luteum begins its development; 3) As already have been described in other species of animals, hyperplasia and hyperthophy of both thecal and granulosa cells is observed, still during the time that the corpus luteum starts its development.
O artigo apresenta resumo em inglês.
RESUMO
Two Sertoli cell tumours in dogs are described, one benigns, the other malignant. They were therefore diagnosed as Sertoli cell adenoma and adenocarcinoma respectively. These cases appear to be the first ones reported in the brazilian veterinary literature. Both tumours failed to produce hyperestrinism, thus suggesting that this be not a constant symptom. Finally the incidence of these neoplasms is statistically discussed.
No presente trabalho são descritos dois tumores de células de Sertoli, em cães, com características morfológicas; um de tumor benigno e outro de maligno. Por esta razão foram denominados, respectivamente, adenoma e adenocarcinoma de células de Sertoli. Os casos descritos parecem constituir os primeiros na literatura veterinária nacional. Em nenhum dos casos havia sintomatologia de hiperestrinismo, o que sugere não ser a mesma de observação constante. Finalmente, discutiu-se estatisticamente o problema, da incidência dêstes tumores, em nosso meio.
RESUMO
75 cases of aortic lesions caused by S. lupi are analized as found in 1661 post mortem of dogs. The gross and microscopic aspect are described and frequency of this parasitosis in São Paulo as well as the biological cycle in the dog and its relation to the lesions observed is discussed. Based on their findings the authors conclude as follows: 1) The incidence of S. lupi in São Paulo is 4.5%. 2) The parasite was found to systematically cause arterial and aesophagic lesions in this town. 3) There is, usually, an aneurisma sacciformis as consequence of a previous inflammatory process in the aorta thoracica. 4) The mesarteric layer is particularly damaged by this process, and there the normal tissue is replaced by connective tissue which is then hyalinized. The fibroid layer is usually hyperplastic. 5) In those cases where ossification of the arterial wall is observed, this process is always found to be membranous.
Os autores estudaram, no presente trabalho, 75 casos de lesões aórticas provocadas por S. lupi, obtidos em 1661 necroscopias de cães. Descrevem macro e microscopicamente as lesões, discutindo a freqüência da parasitose em São Paulo e o ciclo evolutivo do parasita no hospedeiro definitivo em face das lesões observadas. Baseados nas observações, concluem: 1) A freqüência do S. lupi em São, Paulo é de 4,5%. 2) O parasita determina sistemàticamente lesões arteriais e esofágicas, em nosso meio. 3) As lesões na aorta torácica são inicialmente de caráter inflamatório e posteriormente, como seqüela, aparece um aneurisma sacciforme. 4) O processo atinge particularmente a mesartéria, onde o tecido normal é substituído por tecido conjuntivo que se hialiniza. Há também hiperplasia da camada fibróide. 5) Nos casos onde se verifica ossificação da parede arterial, o processo é do tipo membranoso.