RESUMO
Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the influence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented efficiency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytes and osteocytes. With 24 h from co-cultivating adhesion of mesenchymal stem cells in the membranes was observed. There was no foreign body reaction or contamination of surgical wounds and there was intense postoperative neovascularization on seven days. All animals presented omentum adherence, but no adherence to other organs.There was no statistically difference for the different times in macroscopic assessment: deposition of fibrous tissue, implant integration. The same occurred with the microscopic evaluations between the different treatment groups. The groups of immediate and later repair presented different responses to treatment. Discussion: The use of rats as animal model was satisfactory, being suitable for surgical procedures and assessments of the abdominal cavity. The different results obtained between groups of immediate repair and late repair corroborate with the idea that there is difference between induction and repair models in the same surgery or in different surgeries with the time interval between the two, suggesting the need for methodologies that simulate the hernias chronicity. The cells used were classified as mesenchymal stem cells, because it met all the criteria of Mesenchymal and Tissue Stem Cell Committee of the International Society of Celullar Therapy. The membranes conserved with glycerin 98% demonstrated biocompatibility, because there was no rejection or necrosis, infection or exacerbated infl ammation. However the muscle regeneration was not obtained over the membranes - and the methodological difference in other latest experiments about the membranes decellularization and the co-cultivating - can leads to conclusion that the cells attached to membranes were insufficient in number to obtain the desired result. These results suggest the need of new research studies or co-cultivating times and decellularization methods of bovine pericardium for association with mesenchymal stem cells.
Assuntos
Animais , Masculino , Ratos , Pericárdio/transplante , Hérnia Abdominal/reabilitação , Hérnia Abdominal/veterinária , Células-Tronco Mesenquimais , Ratos WistarRESUMO
Atualmente muito se tem discutido sobre os efeitos benéficos da suplementação de ácidos graxos poliinsaturados (AGP), prevenindo ou auxiliando a terapia de várias doenças. O presente estudo visou avaliar a influência da suplementação com AGPs ômega-3 (n-3) veiculados em micro¬partículas na qualidade de cicatrização intestinal de enteroanastomose ileal em ratos. Para o estudo foram utilizados 30 ratos Wistar fêmeas submetidos à celiotomia mediana e enteroanastomose ileal. Os animais foram distribuídos em dois grupos de tratamento: ao grupo 1 (G1) foram administradas micropartículas de n-3, via oral, de acordo com os diferentes períodos de tratamento e ao grupo 2 (G2) administrou-se cetoprofeno como antiinflamatório padrão, pela mesma via, durante 3 dias. Cada grupo foi dividido em 3 subgrupos de acordo com o período de eutanásia, sendo realizada aos 3, 6 ou 8 dias de pós-operatório. Após os tratamentos, os animais foram submetidos à eutaná¬sia, tendo as cavidades abdominais comparadas macroscopicamente quanto a alterações como: presença de secreção, grau de aderência, deiscência da sutura e formação de abscessos. A avaliação foi realizada atribuindo escores de pontuação às diferentes variáveis. O segmento intestinal do sítio de anastomose foi coletado para análise histológica (a ser publicado posteriormente). Com os dados até então obtidos pode-se concluir que a utilização de n-3 veiculado por micropartículas é vantajosa para o processo de cicatrização intestinal pós-operatória quando comparada com a administração de cetoprofeno. mediana e enteroanastomose ileal(AU)
Beneficial effect of long-chain fatty acids in the prevention or therapy of some illnesses has been reported. The aim of this study was to investigate the influence of supplementation with n-3 fatty acids-loaded microparticles in the healing of intestinal anastomosis in rats. Thirty female Wistar rats were submitted to ileal anastomosis. The animals were divided in two groups of treatment. The group 1 (G1) received n-3 fatty acids microparticles by oral route according to different periods of treatment and the group 2 (G2) (control group) received ketoprofen for three days. The groups were divided in 3 sub-groups according to the period of euthanasia, carried out at 3, 6 or 8 postoperative days. After euthanasia, alterations in the abdominal cavity were compared macroscopically (regarding secretion, degree of adhesion, dehiscence of the suture and abcess formation). The intestinal segment of the anastomosis was collected and processed for histological evaluation (this second stage will be published later). The results showed that the use of n-3-loaded microparticles is beneficial for the process of postoperative intestinal healing when compared with the ketoprofen treatment(AU)
Assuntos
Animais , Feminino , Ácidos Graxos Ômega-3/uso terapêuticoRESUMO
Atualmente muito se tem discutido sobre os efeitos benéficos da suplementação de ácidos graxos poliinsaturados (AGP), prevenindo ou auxiliando a terapia de várias doenças. O presente estudo visou avaliar a influência da suplementação com AGPs ômega-3 (n-3) veiculados em micro¬partículas na qualidade de cicatrização intestinal de enteroanastomose ileal em ratos. Para o estudo foram utilizados 30 ratos Wistar fêmeas submetidos à celiotomia mediana e enteroanastomose ileal. Os animais foram distribuídos em dois grupos de tratamento: ao grupo 1 (G1) foram administradas micropartículas de n-3, via oral, de acordo com os diferentes períodos de tratamento e ao grupo 2 (G2) administrou-se cetoprofeno como antiinflamatório padrão, pela mesma via, durante 3 dias. Cada grupo foi dividido em 3 subgrupos de acordo com o período de eutanásia, sendo realizada aos 3, 6 ou 8 dias de pós-operatório. Após os tratamentos, os animais foram submetidos à eutaná¬sia, tendo as cavidades abdominais comparadas macroscopicamente quanto a alterações como: presença de secreção, grau de aderência, deiscência da sutura e formação de abscessos. A avaliação foi realizada atribuindo escores de pontuação às diferentes variáveis. O segmento intestinal do sítio de anastomose foi coletado para análise histológica (a ser publicado posteriormente). Com os dados até então obtidos pode-se concluir que a utilização de n-3 veiculado por micropartículas é vantajosa para o processo de cicatrização intestinal pós-operatória quando comparada com a administração de cetoprofeno. mediana e enteroanastomose ileal
Beneficial effect of long-chain fatty acids in the prevention or therapy of some illnesses has been reported. The aim of this study was to investigate the influence of supplementation with n-3 fatty acids-loaded microparticles in the healing of intestinal anastomosis in rats. Thirty female Wistar rats were submitted to ileal anastomosis. The animals were divided in two groups of treatment. The group 1 (G1) received n-3 fatty acids microparticles by oral route according to different periods of treatment and the group 2 (G2) (control group) received ketoprofen for three days. The groups were divided in 3 sub-groups according to the period of euthanasia, carried out at 3, 6 or 8 postoperative days. After euthanasia, alterations in the abdominal cavity were compared macroscopically (regarding secretion, degree of adhesion, dehiscence of the suture and abcess formation). The intestinal segment of the anastomosis was collected and processed for histological evaluation (this second stage will be published later). The results showed that the use of n-3-loaded microparticles is beneficial for the process of postoperative intestinal healing when compared with the ketoprofen treatment
Assuntos
Feminino , Animais , /uso terapêuticoRESUMO
Neste estudo é apresentado um modelo experimental de defeito agudo em nervo periférico para avaliação da regeneração nervosa mediante técnica de tubulização associada à inoculação de células-tronco autólogas de medula óssea. Foram utilizados 12 coelhos Nova Zelândia albinos, submetidos à secção bilateral e ao afastamento de 5mm do nervo tibial e posterior reparo mediante utilização de câmara de silicone. Internamente à prótese de tubulização do nervo tibial esquerdo em todos os animais, foram inoculadas células-tronco autólogas de medula óssea, coletadas a partir do úmero. Como grupo controle (nervo tibial direito), mediante aplicação da mesma técnica de reparo, solução de NaCl 0,9 por cento foi administrada internamente à prótese. Após 30 dias de observação, os animais foram eutanasiados e foi realizada a avaliação histológica dos segmentos nervosos por meio das colorações de hematoxilina-eosina, luxol fast blue e azul de toluidina. Com os resultados, foi possível concluir que o transplante de células-tronco autólogas associado à técnica de tubulização apresenta vantagens no processo de regeneração nervosa periférica.
This study presents an experimental model of an acute deffect in a peripheral nerve to evaluate neural regeneration using a tubulization technique associated with the inoculation of autologous stem cells from bone marrow. A total of 12 New Zealand white rabbits underwent a bilateral dissection of the tibial nerve followed by repair with silicone tubulization. On the left tibial nerve of all animals, the tube was filled with autologous bone marrow-derived stem cells collected from the humerus. For control, using the same repair technique, the tubes were filled with a NaCl solution in the right tibial nerve. After 30 days of observation, the animals were euthanized and a histological evaluation of the collected nerve segments was performed by staining with hematoxylin-eosin, luxol fast blue, and toluidine blue. From the results it is possible to conclude that the transplanted autologous stem cells associated with the tubulization technique present an advantage in the peripheral nerve regeneration process.