RESUMO
Breast cancer patients may express abnormal cellular immune responses affecting their immunological competence. The analysis of immunological parameters may be useful as indicators of T cell function. To determine the expression of lymphocyte activation proteins and cytokines in tumor and non-metastatic axillary lymph nodes, 30 breast cancer patients were monitored. CD3 polypeptides, PTKs (protein tyrosine kinases) and phosphorylated tyrosines were studied by Western Blot and cytokines mRNA expression was determined by RT-PCR (reverse transcription-polymerase chain reaction). This group of patient had shown high immunohistochemistry expression of IL-10 in tumors. Activation proteins were mainly expressed in involved lymph nodes comparing with their expression in tumors. The differences in expression of CD3 polypeptides and p56(lck) between both locations were significant. There was no statistical association between PTKs and IL-10 in the tumor but more than 50% of cases who express IL-10 lost p56(lck), p59(fyn). A direct association between IL-10 and CD3-polypeptides was observed, however 52.2% of patients who express IL-10 did not express 41 kDa CD3-zeta form. IL-10 mRNA was detected in more than 50% of tumors contrary to the prevalence of type 1 cytokines in regional nodes (40%). The lack of expression of lymphocyte activations proteins and the high expression of IL-10 suggest a downregulation on T cells function in the tumors. These results are useful in order to understand the local immune response that would be key in the control of the tumor progression.
Assuntos
Neoplasias da Mama/genética , Neoplasias da Mama/imunologia , Interleucina-10/genética , Linfócitos T/imunologia , Neoplasias da Mama/patologia , Neoplasias da Mama/cirurgia , Complexo CD3/genética , Complexo CD3/imunologia , Citocinas/genética , Feminino , Humanos , Interferons/genética , Interleucinas/genética , Peptídeos e Proteínas de Sinalização Intracelular/genética , Linfonodos/imunologia , Linfonodos/patologia , Ativação Linfocitária , Mastectomia , Estadiamento de Neoplasias , Proteínas Tirosina Quinases/genética , RNA Polimerase I , RNA Mensageiro/genética , RNA Neoplásico/genética , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Tumor markers are expressed due to molecular alterations of the tumor cells, and we can relate them to the immune system to find new associations to improve prognosis. IL-10 inhibits the generation of immune responses at the tumor site. To determine IL-10 expression in the tumor microenvironment and to associate it with certain tumor markers, 27 breast cancer patients were monitored by immunohistochemistry. The results showed that 23 breast cancer samples exhibited a strong expression of IL-10. IL-10 was associated with some poor prognosis tumor makers. A direct association between IL-10, Bcl-2, and Bax was detected. The relationship between IL-10 and Bax was statistically significant (P=0.001). An inverse association of IL-10 with p53 was observed. IL-10 reflects a suppressive tumor microenvironment, and its relationship with apoptosis markers can suggest an increase in the aggressiveness of the tumor even if it still is at an early stage.
Assuntos
Biomarcadores Tumorais/sangue , Neoplasias da Mama/sangue , Interleucina-10/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Apoptose/imunologia , Neoplasias da Mama/imunologia , Carcinoma Ductal de Mama/sangue , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Prognóstico , Proteínas Proto-Oncogênicas c-bcl-2/sangue , Receptor ErbB-2/sangue , Receptores de Estrogênio/sangue , Proteína Supressora de Tumor p53/sangueRESUMO
Las unidades liticas constituyen en la actualidad una de las formas mas usadas de cuantificacion de la actividad citotoxica natural. En el presente trabajo se utilizaron represiones lineales y no lineales propuestas en la literatura para el calculo de estas, con el objetivo de comparar el comportamiento de dichos modelos. Fueron ajustadas ecuaciones de regresion lineal de: a) por ciento de cromo desprendido vs la cantidad de celulas electoras, b) por ciento de cromo desprendido vs el logaritmo de la cantidad de celulas efectoras y c) arcoseno de la raiz cuadrada de la proporcion de cromo desprendido vs la cantidad de celulas efectoras; asi como una ecuacion no lineal de la forma Y = A* [1/exp(/k*x)], donde Y es el por ciento de Cr desprendido. A la lisis maxima experimental, x la proporcion efectoras-diana y K la pendiente de la recta que se obtiene al plotear In(A-Y) vx x. Los modelos fueron comparados desde un punto de vista estadistico, se utilizaron indicadores de bondad de ajuste, prueba de significacion de la regresion para las ecuaciones lineales y porcentajes de variacion explicados por los modelos, asi como desde un punto de vista biologico a partir de su adecuacion al fenomeno en estudio. El modelo de regresion no lineal usuado mostro ventajas apreciables en este sentido
Assuntos
Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Neoplasias/imunologia , Neoplasias/metabolismo , Testes Imunológicos de Citotoxicidade/métodosRESUMO
Se analizo la accion de las celulas citotoxicas en un grupo de pacientes con neoplasias de colon frente a las lineas celulares Sw-948 y K-562. Se obtuvieron valores de dos tiempos de incubaciones distintos ( 4 y 18 horas) y se estudiaron ademas los marcadores fenotipicos de membrana CD3, CD4, CD6, CD8 y HLA-DR, en celulas linfoides de sangre periferica. Los resultados se compararon con los de un grupo control; solo el grupo de pacientes mostro un incremento significativo de la actividad citotoxica al pasar de 4 a 18 horas de incubacion para ambas lineas celulares, a su vez este grupo expreso niveles bajos de linfocitos CD3 + CD6. Se encontro una correlacion positiva entre estos dos marcadores y la actividad citotoxica