RESUMO
A lectin-like protein was isolated from L. muta venom by gel filtration on BIO Gel P-100 followed by column Chromatography on DEAE-sephades A-50. The protein eluted at 0.4 M Nacl in 0.01 Tris pH 7.3 and exhibited agglutinin activity toward 0+ human erythrocytes. The protein is a dimer with Mr 28 kDa. Amino acid analysis revealed high content of tryptophan and acid recidues and low content of cysteine and methionine residues. No neutral carbohydrates and sialic acid were detected. Circular dichroic spectrum shows 78% of B structure and 1% of alpha structure. In vitro experiments with erythrocytes from rat, rabbit and dog revealed strong agglutination while red blood cells from mice, sheep and goat were not agglutinated. In vivo experiments using anesthetized rats, a sharp and prolonged fall in the blood pressure was observed at protein dose of 1.5 mg/kg. Double dose of protein caused the death of the animal.
Assuntos
Venenos de Crotalídeos/química , Hemaglutininas/isolamento & purificação , Lectinas/isolamento & purificação , Viperidae/metabolismo , Aminoácidos/análise , Animais , Cães , Eritrócitos/efeitos dos fármacos , Cabras/sangue , Hemaglutininas/química , Hemaglutininas/farmacologia , Hemaglutininas/toxicidade , Humanos , Hipotensão/induzido quimicamente , Lectinas/química , Lectinas/farmacologia , Lectinas/toxicidade , Camundongos , Estrutura Secundária de Proteína , Proteínas/química , Proteínas/isolamento & purificação , Proteínas/farmacologia , Proteínas/toxicidade , Coelhos , Ratos , Ratos Sprague-Dawley , Ovinos/sangue , Especificidade da EspécieRESUMO
A lectin-like protein was isolated from L. muta venom by gel filtration on BIO Gel P-100 followed by column Chromatography on DEAE-Sephadex A-50. The protein eluted at 0.4 M NaCl in 0.01 Tris pH 7.3 and exhibited agglutinin activity toward 0**+ human erytrocytes. The protein is a dimer with Mr 28 kDa. Amino acid analysis revealed high content or tryptophan and acid recidues and low content of cysteine and methionine residues. No neural carbohydrates an sialic acid were detected. Circular dichroic spectrum shows 78% of B structure and 1% of alfa structure. In vitro experiments with ery throcytes from rat, rabbit and dog revealed strong agglutination while red blood cells from mice, sheep and goat were not agglutinated. In vivo experiments using non-anesthetized rats, a sharp and prolonged fall in the blood pressure was observed at protein dose of 1.5 mg/kg. Double dose of protein caused the death of the animal