RESUMO
BACKGROUND: Leiomyomas are the most common tumors of the female reproductive tract and a major public health problem. The mechanism of tumorigenesis is unknown, but evidence suggests that estrogens regulate cell proliferation and myoma growth. This effect might be due to different amounts of estrogen receptors (ERα and ERß) in normal and myoma tissues and overexpression of aromatase P450 in myomas. PURPOSE: To assess protein expression of ERs and aromatase in leiomyomas and normal adjacent myometrium of premenopausal women. METHODS: Samples were collected from 12 premenopausal women admitted for abdominal hysterectomy due to fibroids. RESULTS: The protein expression of ERα, ERß and aromatase was similar in leiomyoma and normal myometrium (p = 0.239, p = 0.695 and p = 0.203, respectively). CONCLUSIONS: In this analysis of 12 matched leiomyoma and myometrial samples, the data do not support the theory that overexpression ERα, ERß and aromatase in uterine leiomyomas compared to adjacent myometrium are the cause of tumor growth. The estrogens may exert their growth-stimulatory effects on leiomyomas intermediated by other elements, such as cytokines and growth or apoptosis factors. The effect of estrogen on the growth and development of fibroids is complex and far from being completely understood.
Assuntos
Aromatase/metabolismo , Receptor alfa de Estrogênio/metabolismo , Receptor beta de Estrogênio/metabolismo , Leiomioma/metabolismo , Miométrio/metabolismo , Neoplasias Uterinas/metabolismo , Adulto , Aromatase/genética , Western Blotting , Receptor alfa de Estrogênio/genética , Receptor beta de Estrogênio/genética , Feminino , Expressão Gênica , Humanos , Histerectomia , Leiomioma/cirurgia , Pessoa de Meia-Idade , Pré-Menopausa , Neoplasias Uterinas/cirurgiaRESUMO
PURPOSE: To assess gene and protein expression of progesterone receptor isoforms A and B, cell cycle regulators p53 and p21 in leiomyoma and myometrium. METHODS: Samples were collected from 14 patients in reproductive age who underwent abdominal hysterectomy. Gene expression of PRA, PRB, p53 and p21 was analyzed by real-time PCR. Protein expression was assessed by Western blots. RESULTS: There was no change in gene and protein expression of PRA and PRB in both tissues. The ratio between isoforms (PRA:PRB) was not different between tissues and showed a strong correlation (r = 0.767, P = 0.004). The analysis of gene expression and protein showed increased levels of mRNA and protein p53 in leiomyoma compared to myometrium (P = 0.030 and P = 0.002, respectively). The same increase was observed in p21 mRNA levels (P = 0.016) and protein p21 levels (P = 0.026) in samples of uterine leiomyoma. CONCLUSIONS: PRA:PRB ratio is similar in normal myometrium and leiomyomas. p53 and p21 mRNA and protein levels are increased in leiomyomas.
Assuntos
Leiomioma/genética , Leiomioma/metabolismo , Miométrio/metabolismo , Neoplasias Uterinas/genética , Neoplasias Uterinas/metabolismo , Estudos Transversais , Inibidor de Quinase Dependente de Ciclina p21/genética , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Feminino , Expressão Gênica , Humanos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismo , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismoRESUMO
Os autores relatam o caso de urna criança de 2 anos e 4 meses com queixas ginecológicas (secreção vaginal branca), dor abdominal difusa e pseudopuberdade precoce (telarca). Iniciada investigação em Unidade de Saúde PSF de Porto Alegre, sendo referenciada ao nível secundário onde foi diagnosticada neoplasia ovariana (Tumor de Células da Granulosa Juvenil).
The authors report the case of a 2 year and 4 month old child with gynecological complaints (white vaginal secretion), diffuse abdominal pain and early pseudopuberty (telarch). Initiated research in PSF Health Unit of Porto Alegre, being referred to the secondary level where it was diagnosed ovarian neoplasia (Tumor of cells of the Granulosa Juvenil).
Los autores relatan el caso de un niño de 2 años y 4 meses con quejas ginecológicas (secreción vaginal blanca), dolor abdominal difuso y pseudopuberidad precoz (telarca). Se inició una investigación en Unidad de Salud PSF de Porto Alegre, siendo referenciada al nivel secundario donde fue diagnosticada neoplasia ovárica (Tumor de Células de la Granulosa Juvenil).