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Cell Prolif ; 36(2): 65-73, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12680874

RESUMO

In the current study we present a Gompertzian model for cell growth as a function of cell phenotype using six human tumour cell lines (A-549, NCI-H596, NCI-H520, HT-29, SW-620 and U-251). Monolayer cells in exponential growth at various densities were quantified over a week by sulforhodamine B staining assay to produce cell-growth curves. A Gompertz equation was fitted to experimental data to obtain, for each cell line, three empirical growth parameters (initial cell density, cell-growth rate and carrying capacity - the maximal cell density). A cell-shape parameter named deformation coefficient D (a morphological relationship among spreading and confluent cells) was established and compared by regression analysis with the relative growth rate parameter K described by the Gompertz equation. We have found that coefficient D is directly proportional to the growth parameter K. The fit curve significantly matches the empirical data (P < 0.05), with a correlation coefficient of 0.9152. Therefore, a transformed Gompertzian growth function was obtained accordingly to D. The degree of correlation between the Gompertzian growth parameter and the coefficient D allows a new interpretation of the growth parameter K on the basis of morphological measurements of a set of tumour cell types, supporting the idea that cell-growth kinetics can be modulated by phenotypic organization of attached cells.


Assuntos
Modelos Teóricos , Neoplasias/patologia , Carcinoma/patologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Divisão Celular , Tamanho Celular , Neoplasias do Sistema Nervoso Central/patologia , Neoplasias do Colo/patologia , Glioma/patologia , Humanos , Cinética , Neoplasias Pulmonares/patologia , Fenótipo , Células Tumorais Cultivadas
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