RESUMO
BACKGROUND: Myocardial infarction is a public health problem. Functional food is an alternative treatment for cardiovascular diseases. OBJECTIVE: The objective was to analyze the functional and anatomopathological post-myocardial-infarction effects of soybean extract (SE) and isoflavone (IF). METHODS: Myocardial infarction was induced in adult Wistar rats. After 5 days, an echocardiogram was performed to determine heart rate (HR), ejection fraction (EF), systolic volume (LVESV) and diastolic volume (LVEDV). Animals with ventricular dysfunction (EF<45%) were selected for study. The animals were divided into three groups: control (n=14), SE (n=15) and IF (n=12). The IF group received 120 mg/kg/day isolated IF, and the SE group received 12.52 g/day. After 30 days, a new echocardiogram was performed. A histological exam was carried out to determine the collagen. Activity of biochemical markers [arginase, lactate dehydrogenase (LDH) and malate dehydrogenase] was measured. RESULTS: The animals of the control, IF and SE groups showed a reduction in EF after the infarction (P=.432, P=.017 and P=.320, respectively). An increase of LVESV and LVEDV was observed in all groups (P=.009, P=.001 and P=.140; and P=.003, P=.008 and P=.205, respectively). A reduction of HR was found in the SE group (P=.020). There was a greater activity of LDH in the SE group. A smaller quantity of mature collagen was found in the region proximal to the myocardial infarction in the SE group. CONCLUSION: A protective effect in the SE group was observed 30 days after the myocardial infarction.
Assuntos
Glycine max/química , Isoflavonas/farmacologia , Infarto do Miocárdio/tratamento farmacológico , Infarto do Miocárdio/fisiopatologia , Extratos Vegetais/farmacologia , Animais , Peso Corporal , Colágeno/metabolismo , Ingestão de Alimentos/efeitos dos fármacos , Ecocardiografia , L-Lactato Desidrogenase/metabolismo , Infarto do Miocárdio/patologia , Extratos Vegetais/química , Ratos , Ratos Wistar , Disfunção Ventricular , Função Ventricular Esquerda/efeitos dos fármacosRESUMO
PURPOSE: The aim of the present study was to evaluate the biocompatibility of small intestine submucosa (SIS) in the reconstruction of the ureter in swine. MATERIALS AND METHODS: An experimental study was performed in 10 half-breed pigs weighing between 20 and 30 K, in which a previously prepared segment of SIS measuring approximately 2.0 cm was implanted in the upper third part of the right ureter. RESULTS: Of the 10 operated animals, one died 14 days after the surgery due to a dehiscence on the suture line of the implanted graft. The remaining 9 animals were submitted to ultrasound examination of the urinary tract and were sacrificed on the 40th postoperative day. The macroscopic evaluation showed no calculus, incrustation, fistula, abscesses or adhesions in the ureters with the graft. Microscopic evaluation with hematoxylin-eosin and Sirius red showed in the experimental area (graft) the presence of urothelium in 100 percent of the cases, collagen in 100 percent of the cases, and smooth muscle layer in 87.5 percent of the animals. In the area adjacent to the graft (proximal and distal), we observed 92.86 percent of urothelium, 42.86 percent of collagen and 71.43 percent of smooth muscle. In the contralateral ureter, it was found 100 percent of urothelium and smooth muscle and just 11.11 percent of collagen. The microscopic analysis of the kidneys whose ureters received the graft of SIS evidenced congestion in 55.55 percent, pelvic edema in 66.66 percent and interstitial nephritis in 77.78 percent. Hydronephrosis was present in 33.33 percent and chronic pyelonephritis in 44 percent. Only 1 animal presented total absence of glomerulus in the renal parenchyma. CONCLUSION: The SIS graft behaved as a biological tissue support, allowing the regeneration of the urothelium and smooth muscle grow, despite of chronic inflammatory process.
Assuntos
Animais , Feminino , Masculino , Mucosa Intestinal/cirurgia , Ureter/transplante , Colágeno/metabolismo , Intestino Delgado/cirurgia , Modelos Animais , Músculo Liso/metabolismo , Suínos , Fatores de Tempo , Transplante Homólogo , Resultado do Tratamento , Ureter/metabolismoRESUMO
PURPOSE: The aim of the present study was to evaluate the biocompatibility of small intestine submucosa (SIS) in the reconstruction of the ureter in swine. MATERIALS AND METHODS: An experimental study was performed in 10 half-breed pigs weighing between 20 and 30 K, in which a previously prepared segment of SIS measuring approximately 2.0 cm was implanted in the upper third part of the right ureter. RESULTS: Of the 10 operated animals, one died 14 days after the surgery due to a dehiscence on the suture line of the implanted graft. The remaining 9 animals were submitted to ultrasound examination of the urinary tract and were sacrificed on the 40th postoperative day. The macroscopic evaluation showed no calculus, incrustation, fistula, abscesses or adhesions in the ureters with the graft. Microscopic evaluation with hematoxylin-eosin and Sirius red showed in the experimental area (graft) the presence of urothelium in 100% of the cases, collagen in 100% of the cases, and smooth muscle layer in 87.5% of the animals. In the area adjacent to the graft (proximal and distal), we observed 92.86% of urothelium, 42.86% of collagen and 71.43% of smooth muscle. In the contralateral ureter, it was found 100% of urothelium and smooth muscle and just 11.11% of collagen. The microscopic analysis of the kidneys whose ureters received the graft of SIS evidenced congestion in 55.55%, pelvic edema in 66.66% and interstitial nephritis in 77.78%. Hydronephrosis was present in 33.33% and chronic pyelonephritis in 44%. Only 1 animal presented total absence of glomerulus in the renal parenchyma. CONCLUSION: The SIS graft behaved as a biological tissue support, allowing the regeneration of the urothelium and smooth muscle grow, despite of chronic inflammatory process.
Assuntos
Mucosa Intestinal/cirurgia , Ureter/transplante , Animais , Colágeno/metabolismo , Feminino , Intestino Delgado/cirurgia , Masculino , Modelos Animais , Músculo Liso/metabolismo , Suínos , Fatores de Tempo , Transplante Homólogo , Resultado do Tratamento , Ureter/metabolismoRESUMO
Descriçäo de um caso de laceraçäo completa do esfíncter anal secundária à bestialidade. Um homossexual apresentou laceraçäo completa do esfíncter anal devido à relaçäo sexual com um cäo de 79 kg de peso. O paciente referiu que o pênis do cäo ficou preso travou no seu ânus e que a laceraçäo ocorreu quando o cäo puxou com muita força o seu pênis para fora. Engorgitamento intenso do pênis do cäo pode causar travamento do mesmo dentro do ânus durante relaçäo sexual com o ser humano e pode ser uma causa rara de laceraçäo anal