RESUMO
The fibrinogenolytic activity of Lachesis muta stenophyrs venom was studied. Wistar rats catheterized at carotid artery and jugular vein were inoculated with crude venom or enzyme and changes in arterial pressure, cardiac frequency and electrocardiogram were monitored. The enzyme induced a greater fibrinogen reduction than the crude venom without any cardiovascular or histological alteration. In vitro crude venom coagulated blood whereas the enzyme reduced fibrinogen in 23%. Results suggest the potential use of the fibrinogenolytic enzyme as antithrombotic agent.
Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Fibrinogênio/farmacologia , Fibrinólise , Venenos de Víboras/toxicidade , Viperidae , Análise de Variância , Animais , Pressão Sanguínea/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , Dose Letal Mediana , Masculino , Tempo de Tromboplastina Parcial , Tempo de Protrombina , Ratos , Ratos Wistar , Fatores de Tempo , Venenos de Víboras/enzimologiaRESUMO
A sample of 15 Costa Rican scientific papers published since 1985 reported only 49% of the necessary information about laboratory animals and experimental conditions, slightly better than in the rest of Latin America (47%, sample = 15 papers) but lower than in the USA and Europe (60%, sample = 15). Adequate information on laboratory animals use for biomedical research is important to standardize their use.