RESUMO
A survey for helminth parasites in feral New World non-human primates was conducted and compared with parasitological data from man in the region. A total of 835 fecal samples were collected from feral Aotus nancymae, A. vociferans, Saguinus labiatus, S. mystax, Saimiri boliviensis peruviensis, S. sciureus macrodon, Lagothrix lagotricha and Cacajao calvus rubicundus. In addition, adult parasites were collected from necropsies performed on monkeys that died during quarantine and in captivity. Helminth parasites shared by non-human primates and man in Peru were Ancylostoma braziliensis and Ascaris lumbricoides, found in a captive L. lagotricha; Necator americanus, found in a captive C. calvus rubicundus; Hymenolepis diminuta, found in feral Aotus spp. and S. mystax; and a single Trichuris spp. specimen found in a feral S. sciureus macrodon.
Assuntos
Helmintíase Animal/epidemiologia , Helmintos/fisiologia , Doenças dos Primatas/epidemiologia , Primatas/parasitologia , Animais , Fezes/parasitologia , Humanos , Peru/epidemiologia , PrevalênciaRESUMO
Five tamarins (four Saguinus mystax and one S. labiatus) died with wasting syndrome characterized by chronic diarrhea at the Center for Reproduction and Conservation of Non-Human Primates in Iquitos, Peru. At necropsy, the terminal ileum of all affected tamarins was found to be markedly thickened. Histologically, the terminal ileal mucosa was completely ulcerated, and effaced by debris and mononuclear inflammatory cells. The submucosa and serosa were thickened by fibroplasia, mononuclear cell infiltrates and variable edema. No infectious agent was observed. The lesions were similar to those described previously for Crohn disease. This is to our knowledge the first report of terminal ileitis resembling Crohn disease in non-human primates.
Assuntos
Doença de Crohn/patologia , Doença de Crohn/veterinária , Doenças dos Macacos/patologia , Saguinus , Animais , Doença de Crohn/diagnóstico , Feminino , Histologia , Mucosa Intestinal/patologia , Obstrução Intestinal/patologia , Masculino , Doenças dos Macacos/diagnósticoRESUMO
Allpahuayo virus was initially isolated from arboreal rice rats (Oecomys bicolor and Oecomys paricola) collected during 1997 at the Allpahuayo Biological Station in northeastern Peru. Serological and genetic studies identified the virus as a new member of the Tacaribe complex of the genus Arenavirus. The small (S) segment of the Allpahuayo virus prototype strain CLHP-2098 (Accession No. AY012686) was sequenced, as well as that of sympatric isolate CLHP-2472 (Accession No. AY012687), from the same rodent species. The S segment was 3382 bases in length and phylogenetic analysis indicated that Allpahuayo is a sister virus to Pichinde in clade A. Two ambisense, nonoverlapping reading frames were identified, which result in two predicted gene products, a glycoprotein precursor (GPC) and a nucleocapsid protein (NP). A predicted stable single hairpin secondary structure was identified in the intergenic region between GPC and NP. Details of the genetic organization of Allpahuayo virus are discussed.
Assuntos
Arenavirus/isolamento & purificação , Sigmodontinae/virologia , Sequência de Aminoácidos , Animais , Arenavirus/genética , Arenavirus/imunologia , Sequência de Bases , Testes de Fixação de Complemento , DNA Intergênico , Genoma Viral , Glicoproteínas/genética , Dados de Sequência Molecular , Nucleocapsídeo/genética , Peru , Filogenia , Sorotipagem , Proteínas do Envelope Viral/genéticaRESUMO
Purified rabbit immunoglobulin raised against yeast-expressed recombinant FVO or 3D7 Plasmodium falciparum merozoite surface protein-1 (MSP-1) 19k-D C terminal fragment (MSP-1(19)) was transfused into malaria-naive Aotus nancymai monkeys that were immediately challenged with FVO asexual stage malaria parasites. Control monkeys received rabbit immunoglobulin raised against the sexual stage antigen Pfs25 or Aotus hyperimmune serum obtained from monkeys immunized by P. falciparum infection and drug cure. Passive transfer of rabbit anti-MSP-1(19) failed to protect against homologous or heterologous challenge and, when compared with negative controls, there were no differences in prepatent periods or time to treatment. Interestingly, rabbit anti-MSP-1(19), but not anti-Pfs25, immunoglobulin, and immune monkey serum prevented the development of antibodies directed against MSP-1(19) fragment by infected monkeys, indicating that the antibodies were reactive with native MSP-1(19) antigen in vivo. The prepatent period and time to treatment was greatly delayed in the two monkeys that received Aotus immune serum, both of which developed a chronic intermittent low level infection. In vitro parasite growth inhibition assays (GIAs) confirmed the presence of inhibitory activity (40% maximum inhibition) in concentrated anti-MSP-1(19) immunoglobulin (4.8 mg/ml), but the peak concentrations we achieved in vivo (1 mg/ml) were not inhibitory in vitro. Subinhibitory levels of anti-MSP-1(19) antibodies achieved by passive transfer were not protective against P. falciparum challenge.
Assuntos
Anticorpos Antiprotozoários/imunologia , Imunização Passiva , Proteína 1 de Superfície de Merozoito/imunologia , Plasmodium falciparum/imunologia , Animais , Aotus trivirgatus , Malária Falciparum/prevenção & controle , Plasmodium falciparum/crescimento & desenvolvimento , Coelhos , Proteínas Recombinantes/imunologiaAssuntos
Anticorpos Antiprotozoários/sangue , Antígenos de Protozoários/sangue , Malária Falciparum/veterinária , Doenças dos Macacos/imunologia , Plasmodium falciparum/imunologia , Saimiri/imunologia , Animais , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Malária Falciparum/epidemiologia , Malária Falciparum/imunologia , Masculino , Doenças dos Macacos/epidemiologia , Peru , PrevalênciaRESUMO
Of the 232 tamarins (133 Saguinus mystax and 99 Saguinus labiatus) that died at the Center for Reproduction and Conservation of Nonhuman Primates in Iquitos, Peru from January 1987 to December 1990, 23 monkeys (9.9%) were diagnosed as having chronic colitis. Typically, the cecal and colonic mucosa was greyish and small yellowish cysts, measuring 1-4 mm, were found randomly distributed bulging the mucosa. Microscopically, colitis cystica profunda was diagnosed additionally in six more animals, giving a total of 29 cases (12.5%). This is the first report to our knowledge that describes colitis cystica profunda in a nonhuman primate.
Assuntos
Colite/veterinária , Mucosa Intestinal/patologia , Doenças dos Primatas , Saguinus , Animais , Ceco/patologia , Colite/patologia , Colo/patologia , Feminino , Masculino , PeruRESUMO
A wild-caught adult female Saguinus mystax died after 54 months in captivity. At necropsy, a small reddish zone in the renal cortex of one kidney was shown histologically to be a hemangiosarcoma.
Assuntos
Hemangiossarcoma/veterinária , Neoplasias Renais/veterinária , Doenças dos Macacos/patologia , Saguinus , Animais , Feminino , Hemangiossarcoma/patologia , Neoplasias Renais/patologiaRESUMO
An adult male Callimico goeldii died spontaneously. At necropsy, small whitish foci were found randomly distributed on the liver surface. Histologically, the foci were composed of mixed inflammatory cells with predominant polymorphonuclear cell infiltration and central areas of necrosis. Microbiological cultures revealed a Gram-negative coccoid-bacilli with bipolar staining. Biochemical analysis revealed that the microorganism was Pasteurella haemolytica.
Assuntos
Callimico , Hepatite Animal/patologia , Mannheimia haemolytica , Doenças dos Macacos/patologia , Infecções por Pasteurella/veterinária , Animais , Hepatite Animal/microbiologia , Masculino , Doenças dos Macacos/microbiologia , Infecções por Pasteurella/microbiologia , Infecções por Pasteurella/patologiaRESUMO
A wild-caught male Aotus vociferans died spontaneously during quarantine. Histologic examination of the heart showed several small myocardial arteries replaced with fibrinoid necrosis and adjacent inflammation that included lymphocytes and large histiocytic cells. Less often, similar inflammatory foci were observed in the myocardium interstitium. The lesions observed in the heart of the owl monkey resembled Aschoff bodies.
Assuntos
Aotidae , Cardiomiopatias/veterinária , Doenças dos Macacos/patologia , Cardiopatia Reumática/veterinária , Doença Aguda , Animais , Animais Selvagens , Cardiomiopatias/patologia , Masculino , Cardiopatia Reumática/patologia , Infecções Estreptocócicas/patologia , Infecções Estreptocócicas/veterináriaRESUMO
Clinical and pathologic data were reviewed for 72 owl monkeys that died between January 1987 and May 1990 at the Center for Reproduction and Conservation of Nonhuman Primates in Iquitos, Peru. Tissue samples from 39 animals were examined. Hypertrophic cardiac disease (51% of animals examined), dilative cardiomyopathy (26%), and nephropathy (87%) were the most common diagnoses. The incidence of all three diseases appeared to increase with time in captivity. Nephropathy was less severe in colony-born animals.
Assuntos
Aotidae , Cardiomiopatias/veterinária , Nefropatias/veterinária , Doenças dos Macacos/patologia , Animais , Cardiomiopatias/patologia , Feminino , Nefropatias/patologia , MasculinoRESUMO
From January 1987 to November 1990, 125 adult Saguinus mystax died at the CRCP. Enteritis/colitis (26%), hypoglycemia/cachexia (19%), and parasitic enteritis (13%) were the most common causes of death. Less common were purulent peritonitis (9%), lobular pneumonia (8%), and hemorrhagic gastroenterocolitis (6%). These results confirm the high frequency of gastroenteric lesions reported in Callitrichidae in captivity.
Assuntos
Animais de Zoológico , Gastroenterite/veterinária , Doenças dos Macacos/mortalidade , Saguinus , Animais , Caquexia/mortalidade , Caquexia/veterinária , Causas de Morte , Feminino , Gastroenterite/mortalidade , Hipoglicemia/mortalidade , Hipoglicemia/veterinária , Intussuscepção/mortalidade , Intussuscepção/veterinária , Hepatopatias/mortalidade , Hepatopatias/veterinária , Masculino , Doenças dos Macacos/epidemiologia , Doenças Parasitárias/mortalidade , Doenças Parasitárias em Animais , Peritonite/mortalidade , Peritonite/veterinária , Peru/epidemiologia , Pneumonia/mortalidade , Pneumonia/veterináriaRESUMO
An adult male Aotus nancymae maintained for breeding purposes was submitted for surgery and the left testicle was removed. The surgically removed mass was about two to three times the size of a normal adult owl monkey testicle. Grossly, on cut surface, the mass was soft, white to pale gray, and bulged above the adjacent tissue. Microscopically, the morphology of the tumor cells was consistent with a seminoma.
Assuntos
Aotidae , Disgerminoma/veterinária , Doenças dos Macacos/patologia , Neoplasias Testiculares/veterinária , Animais , Disgerminoma/patologia , Disgerminoma/cirurgia , Masculino , Mitose , Doenças dos Macacos/cirurgia , Necrose , Neoplasias Testiculares/patologia , Neoplasias Testiculares/cirurgiaRESUMO
Two captive-born Saddleback tamarins (Saguinus fuscicollis) died unexpectedly in the primate colony at the Peruvian Primatological Project. At necropsy, a firm, mobile, oblong, obscure mass was discovered in the stomach of each. They were removed and determined to be trichobezoars.