RESUMO

Borrelia burgdorferisensu lato infection has been confirmed in clinical cases in the northeast of Mexico; however, the bacterium has not been identified as infecting the tick vector Ixodes, Amblyomma and Dermacentor ticks were collected from mammals and plants in northeastern Mexico and examined for Borrelia. Eighteen of 214 ticks were PCR-positive for the fla and 16S rRNA genes and 15 for the ospA gene. Southern blotting with a fla probe and sequencing of ospA genes confirmed infection with B. burgdorferi sensu stricto. These findings, together with reports of indigenous cases, fulfil the criteria that allow northeastern Mexico to be considered as a zone endemic for Lyme disease.

Assuntos

Infecções por Borrelia/veterinária , Borrelia burgdorferi/isolamento & purificação , Ixodidae/microbiologia , Animais , Antígenos de Superfície/genética , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/genética , Vacinas Bacterianas/genética , Southern Blotting , Infecções por Borrelia/microbiologia , Borrelia burgdorferi/genética , DNA Bacteriano/genética , Lipoproteínas/genética , México , Reação em Cadeia da Polimerase/métodos , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA