RESUMO
Central nervous system tumors produce adverse outcomes in daily life, although low-grade gliomas are rare in adults. In neurological clinics, the state of impairment of executive functions goes unnoticed in the examinations and interviews carried out. For this reason, the objective of this study was to describe the executive function of a 59-year-old adult neurocancer patient. This study is novel in integrating and demonstrating biological effects and outcomes in performance evaluated by a neuropsychological instrument and psychological interviews. For this purpose, pre- and post-evaluations were carried out of neurological and neuropsychological functioning through neuroimaging techniques (iRM, spectroscopy, electroencephalography), hospital medical history, psychological interviews, and the Wisconsin Card Classification Test (WCST). There was evidence of deterioration in executive performance, as evidenced by the increase in perseverative scores, failure to maintain one's attitude, and an inability to learn in relation to clinical samples. This information coincides with the evolution of neuroimaging over time. Our case shows that the presence of the tumor is associated with alterations in executive functions that are not very evident in clinical interviews or are explicit in neuropsychological evaluations. In this study, we quantified the degree of impairment of executive functions in a patient with low-grade glioma in a middle-income country where research is scarce.
Assuntos
Neoplasias Encefálicas , Função Executiva , Glioma , Testes Neuropsicológicos , Humanos , Função Executiva/fisiologia , Pessoa de Meia-Idade , Glioma/patologia , Glioma/diagnóstico por imagem , Glioma/psicologia , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/patologia , Neoplasias Encefálicas/psicologia , Masculino , Imageamento por Ressonância Magnética/métodos , Eletroencefalografia , FemininoRESUMO
Breast cancer is the most frequent neoplasia in developed countries and the leading cause of death in women worldwide. Epithelial-to-mesenchymal transition (EMT) is a cellular process through which epithelial cells decrease or lose their epithelial characteristics and gain mesenchymal properties. EMT mediates tumor progression, because tumor cells acquire the capacity to execute the multiple steps of invasion and metastasis. Benzo[a]pyrene (B[a]P) is an environmental organic pollutant generated during the burning of fossil fuels, wood, and other organic materials. B[a]P exposition increases the incidence of breast cancer, and induces migration and/or invasion in MDA-MB-231 and MCF-7 breast cancer cells. However, the role of B[a]P in the induction of an EMT process and metastasis of mammary carcinoma cells has not been studied in detail. In this study, we demonstrate that B[a]P induces an EMT process in MCF10A mammary non-tumorigenic epithelial cells. In addition, B[a]P promotes the formation of larger tumors in Balb/cJ mice inoculated with 4T1 cells than in untreated mice and treated with dimethyl sulfoxide (DMSO). B[a]P also increases the number of mice with metastasis to brain and the total number of brain metastatic nodules in Balb/cJ mice inoculated with 4T1 cells compared with untreated mice and treated with DMSO. In conclusion, B[a]P induces an EMT process in MCF10A cells and the growth of mammary tumors and metastasis to brain in Balb/cJ mice inoculated with 4T1 cells.
Assuntos
Benzo(a)pireno , Neoplasias Encefálicas , Transição Epitelial-Mesenquimal , Camundongos Endogâmicos BALB C , Animais , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Feminino , Benzo(a)pireno/toxicidade , Humanos , Camundongos , Neoplasias Encefálicas/secundário , Neoplasias Encefálicas/patologia , Neoplasias Encefálicas/induzido quimicamente , Neoplasias da Mama/patologia , Neoplasias da Mama/induzido quimicamente , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacosRESUMO
Bisphenol A (BPA) is an industrial synthetic chemical used in the production of polycarbonate plastics and epoxy resins. Human exposition to BPA is primarily through eating food, and drinking liquids, because BPA can leach from polycarbonate plastic containers, beverage cans and epoxy resins. BPA induces proliferation and migration in human breast cancer cells. The G protein-coupled estrogen receptor (GPER) is a G protein-coupled receptor coupled with Gs proteins that is activated by estrogen and estrogenic compounds and it is the receptor for BPA. However, the signal transduction pathways that mediate migration via BPA/GPER in triple negative breast cancer (TNBC) cells has not been studied in detail. Here, we demonstrate that BPA induces an increase of GPER expression and activation of FAK, Src and ERK2, and an increase of focal adhesion assembly via GPER in TNBC MDA-MB-231 cells. Moreover, BPA induces FAK and ERK2 activation, focal adhesion assembly and migration via epidermal growth factor receptor (EGFR) transactivation. Collectively our data showed that BPA via GPER and/or EGFR transactivation induces activation of signal transduction pathways that mediate migration in TNBC MDA-MB-231 cells.
Assuntos
Compostos Benzidrílicos/toxicidade , Adesões Focais/efeitos dos fármacos , Fenóis/toxicidade , Plastificantes/toxicidade , Proteínas Tirosina Quinases/metabolismo , Receptores de Estrogênio/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Neoplasias de Mama Triplo Negativas/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Humanos , Receptores de Estrogênio/genética , Receptores Acoplados a Proteínas G/genéticaRESUMO
Bee envenomation is frequent in humans and dogs, but uncommon in horses. This study aimed to describe a case of acute renal failure following mass envenomation in a horse. A mare was attacked by a swarm of bees and showed reluctance to move, epistaxis, and dark-brown urine. Biochemical exams revealed increase in urea and creatine serum levels. The mare did not respond to treatment and euthanasia was elected after four days of clinical course. At the necropsy, there were multifocal pinpoint to elevated skin lesions associated with edema and hemorrhage, which extended to the subcutaneous tissue and skeletal muscle, and the kidneys were diffusely dark-brown and friable. Microscopically, renal tubules were distended and filled with an orange-red, hyaline globular material, and had severe epithelial tubular cell necrosis. The diagnosis was established based on clinical and histological analysis, and pathological evaluation was essential to confirm acute renal failure due to bee sting toxicity.(AU)
Acidentes por picada de abelhas são frequentemente descritos em humanos e cães, entretanto relatos em cavalos são escassos. Este trabalho teve como objetivo descrever um caso de insuficiência renal aguda em um equino após múltiplas picadas de abelhas. Uma égua foi atacada por um enxame de abelhas desenvolvendo quadro clínico de relutância em se movimentar, epistaxe e urina marrom-escura. Exames bioquímicos demonstraram aumento nos níveis séricos de ureia e creatina. O equino não respondeu ao tratamento e a eutanásia foi realizada após quatro dias de curso clínico. Na necropsia havia múltiplas elevações cutâneas, que ao corte exibiam edema e hemorragia, os quais se estendiam ao subcutâneo e musculatura adjacente. Os rins estavam difusamente marrom-escuros e havia friáveis. Microscopicamente, os túbulos renais estavam distendidos e preenchidos por um material hialino, globular, laranja-avermelhado e havia acentuada necrose das células epiteliais tubulares. O diagnóstico foi obtido por meio da análise clínica e histológica, e a avaliação patológica foi essencial para confirmar a insuficiência renal aguda por picada de abelha.(AU)
Assuntos
Animais , Feminino , Cavalos , Mordeduras e Picadas de Insetos/complicações , Mordeduras e Picadas de Insetos/veterinária , Insuficiência Renal/veterinária , Intoxicação/veterinária , AbelhasRESUMO
ABSTRACT: Bee envenomation is frequent in humans and dogs, but uncommon in horses. This study aimed to describe a case of acute renal failure following mass envenomation in a horse. A mare was attacked by a swarm of bees and showed reluctance to move, epistaxis, and dark-brown urine. Biochemical exams revealed increase in urea and creatine serum levels. The mare did not respond to treatment and euthanasia was elected after four days of clinical course. At the necropsy, there were multifocal pinpoint to elevated skin lesions associated with edema and hemorrhage, which extended to the subcutaneous tissue and skeletal muscle, and the kidneys were diffusely dark-brown and friable. Microscopically, renal tubules were distended and filled with an orange-red, hyaline globular material, and had severe epithelial tubular cell necrosis. The diagnosis was established based on clinical and histological analysis, and pathological evaluation was essential to confirm acute renal failure due to bee sting toxicity.
RESUMO: Acidentes por picada de abelhas são frequentemente descritos em humanos e cães, entretanto relatos em cavalos são escassos. Este trabalho teve como objetivo descrever um caso de insuficiência renal aguda em um equino após múltiplas picadas de abelhas. Uma égua foi atacada por um enxame de abelhas desenvolvendo quadro clínico de relutância em se movimentar, epistaxe e urina marrom-escura. Exames bioquímicos demonstraram aumento nos níveis séricos de ureia e creatina. O equino não respondeu ao tratamento e a eutanásia foi realizada após quatro dias de curso clínico. Na necropsia havia múltiplas elevações cutâneas, que ao corte exibiam edema e hemorragia, os quais se estendiam ao subcutâneo e musculatura adjacente. Os rins estavam difusamente marrom-escuros e havia friáveis. Microscopicamente, os túbulos renais estavam distendidos e preenchidos por um material hialino, globular, laranja-avermelhado e havia acentuada necrose das células epiteliais tubulares. O diagnóstico foi obtido por meio da análise clínica e histológica, e a avaliação patológica foi essencial para confirmar a insuficiência renal aguda por picada de abelha.
RESUMO
Foi atendido no Hospital Veterinário Jockey (HVJ) , localizado no Jockey Clube do Rio Grande do Sul, um equino da raça Quarto de Milha, 4 meses de idade, apresentando dor ocular, lacrimejamento, blefaroespasmo e ausência de resposta a estímulos visuais. Além disso, o animal apresentava uma placa de coloração amarelada sobre a córnea do olho esquerdo. O início do tratamento foi realizado na propriedade com o uso tópico de pomada antifúngica e uso sistêmico de anti-inflamatório. Devida ausência de progressão do quadro, foi encaminhada para o hospital, onde foi realizado o tratamento cirúrgico - flap conjuntival unipediculado. Após a cirurgia o animal recebeu terapia anti-inflamatória e antifúngica. Recebeu alta após um mês de tratamento tendo retorno da visão.(AU)
It was attended in the Jockey Veterinary Hospital situated in Jockey Club of Rio Grande do Sul, one foal of the quarter horse breed of four months of life showing pain ocular, tearing, blepharospasm, of color yellowish corneal abscess in the left eye, and did not respond to visual stimulation. The beginning of treatment was performed on the property with antifungal and anti-inflammatory ointment. However, no progression of the condition was referred to the hospital for surgical treatment - conjunctival flap unipediculado. After the surgery still received anti-inflammatory and antifungal therapy. He was discharged after a month of treatment with vision feedback.(AU)
Fue atendido en el Hospital Veterinario Jockey, situado en el Jockey Clube del Rio Grande do Sul, una potra de raza Cuarto de Milla de 4 meses presentando dolor ocular, lagrimeo, blefaroespasmo, abceso corneal de color amarillento en el ojo izquierdo, sin respuesta a estímulos visuales. El comienzo del tratamiento fue realizado en la propiedad con la terapia antifúngica y antiinflamatoria. Sin mejora clínica, fue llevada al hospital para el tratamiento de cirugía - flap conjuntival unipediculado. Después de la cirugía, el tratamiento se continuó con antiinflamatoria y antifúngica. Recibió alta después de un mes de tratamiento, teniendo retorno de la visión.(AU)
Assuntos
Animais , Feminino , Olho/patologia , Cavalos/parasitologia , Infecções Oculares Fúngicas/veterinária , Procedimentos Cirúrgicos Oftalmológicos/veterinária , Antifúngicos/uso terapêutico , Anti-Inflamatórios/uso terapêuticoRESUMO
Foi atendido no Hospital Veterinário Jockey (HVJ) , localizado no Jockey Clube do Rio Grande do Sul, um equino da raça Quarto de Milha, 4 meses de idade, apresentando dor ocular, lacrimejamento, blefaroespasmo e ausência de resposta a estímulos visuais. Além disso, o animal apresentava uma placa de coloração amarelada sobre a córnea do olho esquerdo. O início do tratamento foi realizado na propriedade com o uso tópico de pomada antifúngica e uso sistêmico de anti-inflamatório. Devida ausência de progressão do quadro, foi encaminhada para o hospital, onde foi realizado o tratamento cirúrgico - flap conjuntival unipediculado. Após a cirurgia o animal recebeu terapia anti-inflamatória e antifúngica. Recebeu alta após um mês de tratamento tendo retorno da visão.
It was attended in the Jockey Veterinary Hospital situated in Jockey Club of Rio Grande do Sul, one foal of the quarter horse breed of four months of life showing pain ocular, tearing, blepharospasm, of color yellowish corneal abscess in the left eye, and did not respond to visual stimulation. The beginning of treatment was performed on the property with antifungal and anti-inflammatory ointment. However, no progression of the condition was referred to the hospital for surgical treatment - conjunctival flap unipediculado. After the surgery still received anti-inflammatory and antifungal therapy. He was discharged after a month of treatment with vision feedback.
Fue atendido en el Hospital Veterinario Jockey, situado en el Jockey Clube del Rio Grande do Sul, una potra de raza Cuarto de Milla de 4 meses presentando dolor ocular, lagrimeo, blefaroespasmo, abceso corneal de color amarillento en el ojo izquierdo, sin respuesta a estímulos visuales. El comienzo del tratamiento fue realizado en la propiedad con la terapia antifúngica y antiinflamatoria. Sin mejora clínica, fue llevada al hospital para el tratamiento de cirugía - flap conjuntival unipediculado. Después de la cirugía, el tratamiento se continuó con antiinflamatoria y antifúngica. Recibió alta después de un mes de tratamiento, teniendo retorno de la visión.
Assuntos
Feminino , Animais , Cavalos/parasitologia , Infecções Oculares Fúngicas/veterinária , Olho/patologia , Anti-Inflamatórios/uso terapêutico , Antifúngicos/uso terapêutico , Procedimentos Cirúrgicos Oftalmológicos/veterináriaRESUMO
This study compared the correlates of HIV risk among men who have sex with men (MSM) with newly diagnosed versus previously known HIV infection among 5,148 MSM recruited using modified snowball sampling in 5 Peruvian cities. Participants, if age ≥18 years and reporting sex with a male in the previous 12 months, underwent standardized computer-assisted risk assessments and HIV and syphilis testing. Overall, 420 (8.2 %) participants tested HIV seropositive, most of whom (89.8 %) were unaware of their HIV status. Compared to those who knew themselves to be HIV-infected, multivariate logistic regression demonstrated that unprotected anal intercourse at last encounter [AOR = 2.84 (95 % CI 1.09-7.40)] and having an alcohol use disorder (AUD) [AOR = 2.14 (95 % CI 1.01-5.54)] were independently associated with a newly diagnosed HIV infection. Being unaware of being HIV-infected was associated with high-risk sexual behaviors and AUDs, both of which are amenable to behavioral and medication-assisted therapy interventions.
Assuntos
Infecções por HIV/epidemiologia , Soropositividade para HIV/psicologia , Promoção da Saúde/métodos , Homossexualidade Masculina/psicologia , Comportamento Sexual , Alcoolismo/epidemiologia , Aconselhamento , Estudos Transversais , Infecções por HIV/diagnóstico , Infecções por HIV/prevenção & controle , Inquéritos Epidemiológicos , Humanos , Masculino , Peru/epidemiologia , Vigilância da População , Fatores de Risco , Comportamento Sexual/estatística & dados numéricos , Infecções Sexualmente Transmissíveis , Sífilis/diagnóstico , Sífilis/epidemiologia , Sífilis/prevenção & controleRESUMO
BACKGROUND: Sub-national analyses of causes of death and time-trends help to define public health policy priorities. They are particularly important in countries undergoing epidemiological transition like Peru. There are no studies exploring Peruvian national and regional characteristics of such epidemiological transition. We aimed to describe Peru's national and regional mortality profiles between 1996 and 2000. METHODS: Registered mortality data for the study period were corrected for under-registration following standardized methods. Main causes of death by age group and by geographical region were determined. Departmental mortality profiles were constructed to evaluate mortality transition, using 1996 data as baseline. Annual cumulative slopes for the period 1996-2000 were estimated for each department and region. RESULTS: For the study period non-communicable diseases explained more than half of all causes of death, communicable diseases more than one third, and injuries 10.8% of all deaths. Lima accounted for 32% of total population and 20% of total deaths. The Andean region, with 38% of Peru's population, accounted for half of all country deaths. Departmental mortality predominance shifted from communicable diseases in 1996 towards non-communicable diseases and injuries in 2000. Maternal and perinatal conditions, and nutritional deficiencies and nutritional anaemia declined markedly in all departments and regions. Infectious diseases decreased in all regions except Lima. In all regions acute respiratory infections are a leading cause of death, but their proportion ranged from 9.3% in Lima and Callao to 15.3% in the Andean region. Tuberculosis and injuries ranked high in Lima and the Andean region. CONCLUSION: Peruvian mortality shows a double burden of communicable and non-communicable, with increasing importance of non-communicable diseases and injuries. This challenges national and sub-national health system performance and policy making.
Assuntos
Causas de Morte/tendências , Mortalidade da Criança/tendências , Doenças Transmissíveis/mortalidade , Expectativa de Vida/tendências , Sistema de Registros , Adulto , Doenças Cardiovasculares/mortalidade , Pré-Escolar , Doença Crônica , Doenças Transmissíveis/epidemiologia , Países em Desenvolvimento , Doenças do Sistema Digestório/mortalidade , Feminino , Saúde Global , Inquéritos Epidemiológicos , Humanos , Lactente , Mortalidade Infantil/tendências , Recém-Nascido , Masculino , Análise Multivariada , Neoplasias/mortalidade , Distúrbios Nutricionais/mortalidade , Peru/epidemiologia , Pobreza , Prevalência , Probabilidade , Saúde Pública , Medição de Risco , Tuberculose/mortalidadeRESUMO
BACKGROUND: Information on profiles for under-five causes of death is important to guide choice of child-survival interventions. Global level data have been published, but information at country level is scarce. We aimed at defining national and departmental trends and profiles of under-five mortality in Peru from 1996 through 2000. METHODS: We used the Ministry of Health registered under-five mortality data. For correction of under-registration, a model life-table that fitted the age distribution of the population and of registered deaths was identified for each year. The mortality rates corresponding to these model life-tables were then assigned to each department in each particular year. Cumulative reduction in under-five mortality rate in the 1996-2000 period was estimated calculating the annual reduction slope for each department. Departmental level mortality profiles were constructed. Differences in mortality profiles and in mortality reduction between coastal, andean and jungle regions were also assessed. RESULTS: At country level, only 4 causes (pneumonia, diarrhoea, neonatal diseases and injuries) accounted for 68% of all deaths in 1996, and for 62% in 2000. There was 32.7% of under-five death reduction from 1996 to 2000. Diarrhoea and pneumonia deaths decreased by 84.5% and 41.8%, respectively, mainly in the andean region, whereas deaths due to neonatal causes and injuries decreased by 37.2% and 21.7%. For 1996-2000 period, the andean, coast and jungle regions accounted for 52.4%, 33.1% and 14.4% of deaths, respectively. These regions represent 41.0%, 46.4% and 12.6% of under-five population. Both diarrhoea and pneumonia constitute 30.6% of under-five deaths in the andean region. As a proportion, neonatal deaths remained stable in the country from 1996 to 2000, accounting for about 30% of under-five deaths, whereas injuries and "other" causes, including congenital anomalies, increased by about 5%. CONCLUSION: Under-five mortality declined substantially in all departments from 1996 to 2000, which is explained mostly by reduction in diarrhoea and pneumonia deaths, particularly in the andean region. There is the need to emphasize interventions to reduce neonatal deaths and emerging causes of death such as injuries and congenital anomalies.
Assuntos
Causas de Morte/tendências , Mortalidade da Criança/tendências , Política de Saúde , Mortalidade Infantil/tendências , Pré-Escolar , Anormalidades Congênitas/mortalidade , Diarreia/mortalidade , Feminino , Geografia , Humanos , Lactente , Recém-Nascido , Tábuas de Vida , Masculino , Peru/epidemiologia , Pneumonia/mortalidade , Administração em Saúde Pública , Sistema de Registros , Ferimentos e Lesões/mortalidadeRESUMO
The Multi-Country Evaluation of Integrated Management of Childhood Illness (IMCI) Effectiveness, Cost and Impact (MCE) was launched to assess the global effectiveness of this strategy. Impact evaluations were started in five countries. The objectives of the Peru MCE were: (1) to document trends in IMCI implementation in the 24 departments of Peru from 1996 to 2000; (2) to document trends in indicators of health services coverage and impact (mortality and nutritional status) for the same period; (3) to correlate changes in these two sets of indicators, and (4) to attempt to rule out contextual factors that may affect the observed trends and correlations. An ecological analysis was performed in which the units of study were the 24 departments. By 2000, 10.2% of clinical health workers were trained in IMCI, but some districts showed considerably higher rates. There were no significant associations between clinical IMCI training coverage and indicators of outpatient utilization, vaccine coverage, mortality or malnutrition. The lack of association persisted after adjustment for several contextual factors including socioeconomic and environmental indicators and the presence of other child health projects. Community health workers were also trained in IMCI, and training coverage was not associated with any of the process or impact indicators, except for a significant positive correlation with mean height for age. According to the MCE impact model, IMCI implementation must be sufficiently strong to lead to an impact on health and nutrition. Health systems support for IMCI implementation in Peru was far from adequate. This finding, along with low training coverage level and a relatively low child mortality rate, may explain why the expected impact was not documented. Nevertheless, even districts with high levels of training coverage failed to show an impact. Further national effectiveness studies of IMCI and other child interventions are warranted as these interventions are scaled up.
Assuntos
Prestação Integrada de Cuidados de Saúde/organização & administração , Difusão de Inovações , Indicadores Básicos de Saúde , Criança , Serviços de Saúde da Criança , Pré-Escolar , Humanos , Peru/epidemiologia , Atenção Primária à SaúdeRESUMO
Objetivos: 1) Documentar las tendencias en la implementación de la atención integrada a las enfermedades prevalentes de la infancia (Aiepi) en los 24 departamentos del país, de 1996 al 2000. 2) Documentar las tendencias en los indicadores de cobertura de servicios de salud y en los de impacto (mortalidad y estado nutricional) para el mismo período. 3) Correlacionar los cambios en estos dos grupos de indicadores. Y, 4) intentar descartar factores contextuales que puedan afectar las tendencias y las correlaciones observadas. Materiales y Métodos: Se realizó un análisis ecológico en el que las unidades de estudio fueron los 24 departamentos. Resultados: Para el 2000, 10,2 por ciento de trabajadores clínicos (médicos y enfermeras) fueron capacitados en Aiepi, pero solo algunos departamentos mostraron tasas considerablemente mayores. No hubo asociaciones significativas entre la cobertura de capacitación clínica en Aiepi y los indicadores de utilización de consultas externas, cobertura de vacunas, mortalidad o desnutrición. La falta de asociación persistió luego de haber realizado el ajuste de varios factores contextuales incluyendo indicadores socioeconómicos y ambientales y la presencia de otros proyectos de salud del niño. Los agentes comunitarios de salud también fueron capacitados en Aiepi y la cobertura de capacitación no estuvo asociada con ninguno de los indicadores de proceso o de impacto, excepto una correlación positiva y significativa con el promedio de talla para edad. De acuerdo al modelo de impacto de la Evaluación Multi-País del Impacto, Costo y Efectividad de Aiepi (EMP), la implementación de Aiepi debe ser lo suficientemente fuerte para llevar a un impacto en la salud y la nutrición. Conclusiones: El soporte de los sistemas de salud para la implementación de Aiepi en el Perú estuvo lejos de ser adecuada, y esto, así como coberturas de capacitación relativamente bajas, pueden explicar porqué no se documentó el impacto esperado. Sin embargo, inclus...
Assuntos
Peru , Impactos da Poluição na Saúde , Assistência Integral à Saúde , Proteção da Criança , Saúde PúblicaRESUMO
In eukaryotes, pre-rRNA processing depends on a large number of nonribosomal trans-acting factors that form large and intriguingly organized complexes. A novel nucleolar protein, Nop53p, was isolated by using Nop17p as bait in the yeast two-hybrid system. Nop53p also interacts with a second nucleolar protein, Nip7p. A carbon source-conditional strain with the NOP53 coding sequence under the control of the GAL1 promoter did not grow in glucose-containing medium, showing the phenotype of an essential gene. Under nonpermissive conditions, the conditional mutant strain showed rRNA biosynthesis defects, leading to an accumulation of the 27S and 7S pre-rRNAs and depletion of the mature 25S and 5.8S mature rRNAs. Nop53p did not interact with any of the exosome subunits in the yeast two-hybrid system, but its depletion affects the exosome function. In pull-down assays, protein A-tagged Nop53p coprecipitated the 27S and 7S pre-rRNAs, and His-Nop53p also bound directly 5.8S rRNA in vitro, which is consistent with a role for Nop53p in pre-rRNA processing.
Assuntos
Proteínas Nucleares/metabolismo , Proteínas Ribossômicas/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Genes Fúngicos , Humanos , Dados de Sequência Molecular , Mutação , Proteínas Nucleares/genética , Precursores de RNA/genética , Precursores de RNA/metabolismo , Processamento Pós-Transcricional do RNA , RNA Fúngico/genética , RNA Fúngico/metabolismo , RNA Ribossômico 5,8S/genética , RNA Ribossômico 5,8S/metabolismo , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Ribossômicas/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Homologia de Sequência de Aminoácidos , Especificidade da Espécie , Técnicas do Sistema de Duplo-HíbridoRESUMO
In eukaryotes, pre-rRNA processing depends on cis-acting elements and on a large number of non-ribosomal trans-acting factors, including endonucleases and exonucleases, RNA helicases, rRNA modifying enzymes and components of snoRNPs. The exosome is a conserved eukaryotic protein complex containing multiple 3'-5' exonucleases, which has been implicated in pre-rRNA, snoRNA and snRNA processing, as well as in mRNA degradation. In order to identify new proteins involved in rRNA processing, we have screened a yeast two-hybrid cDNA library, to isolate proteins interacting with the exosome subunit Rrp43p. In this screen, a novel nucleolar protein, Nop17p, was identified which also interacts with the box C/D snoRNP protein Nop58p. The NOP17 gene is not essential for cell viability but its deletion causes a temperature-sensitive phenotype. Pre-rRNA processing analyses revealed that rRNA formation is affected in the Deltanop17 strain subjected to the non-permissive temperature, although it is not blocked completely. In addition, primer extension analyses of RNA isolated from Nop17p-depleted cells subjected to the non-permissive temperature indicates that the pre-rRNA is undergoing different modification or degradation processes in these cells as compared to the parental strain. Nop17p was recently described in the same complex as Nop58p and, interestingly, its depletion leads to mislocalization of Nop1p, Nop56p, Nop58p and Snu13p, which are the core proteins of the box C/D ribonucleoprotein (snoRNP), indicating that Nop17p function is required either for nucleolar retention or for the proper assembly of the box C/D snoRNP.
Assuntos
Proteínas Nucleares/metabolismo , Ribonucleoproteínas Nucleolares Pequenas/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Exorribonucleases/metabolismo , Substâncias Macromoleculares , Proteínas Nucleares/fisiologia , Ligação Proteica , Precursores de RNA/metabolismo , Ribonucleoproteínas Nucleares Pequenas/metabolismo , Ribonucleoproteínas Nucleolares Pequenas/biossíntese , Proteínas de Saccharomyces cerevisiae/fisiologia , TemperaturaRESUMO
El presente estudio compara el estado gingival y la profundidad al sondeado como signos principales de la cicatrización y complicaciones post quirúrgicas en pacientes tratados quirúrgicamente mediante colgajos a grosor completo que usaron y no usaron colutorios orales antes y después de cirugías periodontales. Se tomaron 34 pacientes de ambos sexos, de los cuales 17 realizaron enjuagatorios orales con clorhexidina, antes y después de la cirugía periodontal y los otros 17 solamente realizaron cepillado dental luego de la cirugía periodontal. Se encontró que la condición gingival mejora y la profundidad al sondeado disminuye considerablemente en los pacientes que recibieron colutorios de clorhexidina antes y después del tratamiento quirúrgico periodontal, frente a los pacientes que no recibieron dicho enjuagatorio. Esto sugiere que el colutorio de clorhexidina favorece el proceso de cicatrización en este tipo de tratamiento periodontal.
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Feminino , Doenças Periodontais , Complicações Pós-Operatórias , Clorexidina , Antissépticos Bucais , Retalhos CirúrgicosRESUMO
Rrp43p is a Saccharomyces cerevisiae exosome subunit involved in pre-rRNA processing which is found both in the nucleus and in the cytoplasm. So far, no function has been assigned to the cytoplasmic fraction of Rrp43p. We have addressed Rrp43p function by analyzing mRNA stability in three rrp43 temperature-sensitive (ts) strains, which carry different ts alleles (rrp43-1, rrp43-2 and rrp43-3), and by analyzing Rrp43p interactions with the remaining exosome subunits. In the ts strains, endogenous mRNAs (ACT1 and PAB1), as well as a heterologous reporter mRNA (CATpG) showed longer half-lives, relative to a control strain carrying wild-type RRP43. The mutants also accumulated a degradation intermediate of the reporter mRNA that is typical of defective mRNA decay. These results allow us to propose that Rrp43p is required for mRNA degradation. Rrp43p interacts with the exosome complex via Rrp46p, as determined by two-hybrid analyses. Interestingly, the rrp43 ts mutant proteins do not interact with Rrp46p, indicating that the ts phenotype may be caused by disruption of the Rrp43p- Rrp46p interaction. The ts strains also showed a pre-rRNA processing defect, which is consistent with previous studies on Rrp43p function.
Assuntos
Proteínas Fúngicas/metabolismo , RNA Mensageiro/metabolismo , Substituição de Aminoácidos , Northern Blotting , Proteínas Fúngicas/genética , Mutação , Fenótipo , Ligação Proteica , Processamento Pós-Transcricional do RNA , RNA Mensageiro/genética , RNA Ribossômico/genética , RNA Ribossômico/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Temperatura , Técnicas do Sistema de Duplo-HíbridoRESUMO
actualmente el programa "Tomando Decisiones" está probando nuevas estrategias en el departamento de Oruro; y se desarrolla con los siguientes componentes: Educación sexsual para adolescentes; Difusión de los conocimientos; Acceso a centros de salud; Defensoría de la juventud; y Producción de materiales de IEC
Assuntos
Humanos , Serviços de Saúde do Adolescente , Educação Sexual , Materiais de Ensino , BolíviaRESUMO
Tengamos confianza en las facultades de adaptación de la realidad que sabe a veces conciliar lo inconciliable. Sea lo que fuere, cada vez que oigamos decir "uan de dos", apresurémomonos a pensar que, de las dos, la mejor puede ser la tercera
Assuntos
Humanos , Epidemiologia , BolíviaRESUMO
Con el auspicio de Save The Children Bolivia y el Departamento de Comunicación para Población de la Universidad de Jonhs Hopkins, se realizó en el mes de julio de 1997, un taller internacional de movilizacióncomunitaria para la salud. Con los once países de América, se discutieron entre otros, aspectos conceptuales e ideológicos. Por la importancia y repercusiones que estos conceptos pueden tener en la formulación de proyectos de movilización comunitaria, se transcriben los más destacados a partir de los informes de relatoría elaborados durante el taller