Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Genome Res ; 20(11): 1534-44, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20829224

RESUMO

Here, we fully characterize the genomes of 14 Plasmodium falciparum patient isolates taken recently from the Iquitos region using genome scanning, a microarray-based technique that delineates the majority of single-base changes, indels, and copy number variants distinguishing the coding regions of two clones. We show that the parasite population in the Peruvian Amazon bears a limited number of genotypes and low recombination frequencies. Despite the essentially clonal nature of some isolates, we see high frequencies of mutations in subtelomeric highly variable genes and internal var genes, indicating mutations arising during self-mating or mitotic replication. The data also reveal that one or two meioses separate different isolates, showing that P. falciparum clones isolated from different individuals in defined geographical regions could be useful in linkage analyses or quantitative trait locus studies. Through pairwise comparisons of different isolates we discovered point mutations in the apicoplast genome that are close to known mutations that confer clindamycin resistance in other species, but which were hitherto unknown in malaria parasites. Subsequent drug sensitivity testing revealed over 100-fold increase of clindamycin EC(50) in strains harboring one of these mutations. This evidence of clindamycin-resistant parasites in the Amazon suggests that a shift should be made in health policy away from quinine + clindamycin therapy for malaria in pregnant women and infants, and that the development of new lincosamide antibiotics for malaria should be reconsidered.


Assuntos
Instabilidade Cromossômica , Mapeamento Cromossômico , Clindamicina , Resistência a Medicamentos/genética , Malária Falciparum/parasitologia , Plasmodium falciparum/genética , Antimaláricos/uso terapêutico , Sequência de Bases , Instabilidade Cromossômica/genética , Mapeamento Cromossômico/métodos , Clindamicina/uso terapêutico , Variações do Número de Cópias de DNA , Feminino , Frequência do Gene , Genoma de Protozoário , Genótipo , Humanos , Lactente , Malária Falciparum/diagnóstico , Malária Falciparum/tratamento farmacológico , Malária Falciparum/genética , Masculino , Modelos Biológicos , Modelos Moleculares , Dados de Sequência Molecular , Linhagem , Peru , Gravidez , Telômero/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA