RESUMO
The purification of different serotypes of dengue virus from a monoclonal antibody which recognizes an epitope present in the four serotypes bond to a Sepharose 4B matrix activated with cyanogen bromide, is reported. Results evidence that the method employed makes quicker the process of purification with a high degree of purity.
Assuntos
Anticorpos Monoclonais/imunologia , Antígenos Virais/isolamento & purificação , Vírus da Dengue/imunologia , Vírus da Dengue/isolamento & purificação , Animais , Anticorpos Monoclonais/isolamento & purificação , Complexo Antígeno-Anticorpo/imunologia , Antígenos Virais/imunologia , Cromatografia de Afinidade , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Humanos , Hibridomas/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Sensibilidade e EspecificidadeRESUMO
The obtention of two monoclonal antibodies which recognize a single epitope present in the subunit B of the thermolabile toxin of Vibrio cholerae and another which shows a cross-reaction with those produced by certain enteropathogenic toxins, is reported. The standardization of a solid phase indirect immunoenzymatic assay (ELISA) for the primary screening and selection of hybrids was performed; in addition, the isotype was determined.