RESUMO
To investigate the role of the transient receptor potential channel vanilloid type 1 (TRPV1) in hepatic glucose metabolism, we analyzed genes related to the clock system and glucose/lipid metabolism and performed glycogen measurements at ZT8 and ZT20 in the liver of C57Bl/6J (WT) and Trpv1 KO mice. To identify molecular clues associated with metabolic changes, we performed proteomics analysis at ZT8. Liver from Trpv1 KO mice exhibited reduced Per1 expression and increased Pparα, Pparγ, Glut2, G6pc1 (G6pase), Pck1 (Pepck), Akt, and Gsk3b expression at ZT8. Liver from Trpv1 KO mice also showed reduced glycogen storage at ZT8 but not at ZT20 and significant proteomics changes consistent with enhanced glycogenolysis, as well as increased gluconeogenesis and inflammatory features. The network propagation approach evidenced that the TRPV1 channel is an intrinsic component of the glucagon signaling pathway, and its loss seems to be associated with increased gluconeogenesis through PKA signaling. In this sense, the differentially identified kinases and phosphatases in WT and Trpv1 KO liver proteomes show that the PP2A phosphatase complex and PKA may be major players in glycogenolysis in Trpv1 KO mice.
Assuntos
Gluconeogênese , Proteoma , Canais de Cátion TRPV , Animais , Expressão Gênica , Gluconeogênese/genética , Glucose/metabolismo , Glicogênio/metabolismo , Fígado/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteoma/metabolismo , Canais de Cátion TRPV/genética , Canais de Cátion TRPV/metabolismoRESUMO
A rise in plasma triacylglycerol levels is a common physiological occurrence during late gestation and excess of glucocorticoids (GCs) has been shown to impair lipid metabolism. Based on those observations, we investigated whether the administration of dexamethasone during the late gestational period could exacerbate this pregnancy associated hypertriacylglycerolemia in rats. For this, female Wistar rats were treated with dexamethasone (0.2 mg/kg of body mass in the drinking water on days 14-19 of pregnancy; DP group) or equivalent days in the virgin rats (DV group). Untreated pregnant rats (control pregnant group) and age-matched virgin rats (control virgin group) were used as controls. Functional, biochemical, and molecular analyses were carried out after treatment with GC and in the control groups. Euthanasia was performed on day 20 of pregnancy. The metabolic parameters of the mothers (dams) at the time of weaning and 6 months later, as well as newborn survival, were evaluated. We observed that neither dexamethasone nor pregnancy affected blood glucose or glucose tolerance. Hypertriacylglycerolemia associated with lipid intolerance or reduced hepatic triacylglycerol clearance was observed during the late gestational period. GC treatment caused a further increase in basal plasma triacylglycerol levels, but did not have a significant effect on lipid tolerance and hepatic triacylglycerol clearance in pregnant rats. GC, but not pregnancy, caused few significant changes in mRNA expression of proteins involved in lipid metabolism. Dexamethasone during pregnancy had no impact on lipid metabolism later in the dams' life; however, it led to intra-uterine growth restriction and reduced pup survival rate. In conclusion, GC exposure during the late gestational period in rats has no major impact on maternal lipid homeostasis, soon after parturition at weaning, or later in the dams' life, but GC exposure is deleterious to the newborn when high doses are administered at late gestation. These data highlight the importance of performing an individualized and rigorous control of a GC treatment during late pregnancy considering its harmful impact on the fetuses' health.
RESUMO
Embora a incontinência urinária não coloque diretamente a vida das pessoas em risco, é uma condição quepode trazer sérias implicações. Publicações recentes têm demonstrado melhora significativa no quadro clínicoe na qualidade de vida de mulheres incontinentes submetidas ao tratamento conservador. Portanto, o presenteestudo teve como objetivo avaliar o efeito do tratamento fisioterapêutico sobre a qualidade de vida de umamulher com incontinência urinária. Apresentação do caso: estudo de caso de uma mulher com incontinênciaurinária de esforço. Inicialmente foi realizada anamnese e exame físico, coletando-se dados como histórico dadoença e tonicidade dos músculos do assoalho pélvico; em seguida a paciente respondeu ao questionárioInternational Consultation on Incontinence Questionnaire - Short Form. Após 20 sessões de fisioterapiao mesmo exame físico e o mesmo questionário foram aplicados e os escores iniciais e finais, bem como osdados coletados nos exames físicos foram comparados. Resultados: a paciente apresentou aumento notônus muscular do assoalho pélvico e aumento no grau de força dos mesmos, melhora do quadro clínico e dasua qualidade de vida. Conclusão: O presente estudo concluiu que a fisioterapia, baseada em cinesioterapiae eletroterapia endovaginal, contribuiu para a melhora no quadro de incontinência urinária e, conseqüentemente,na melhora da qualidade de vida de paciente tratada.
Even though urinary incontinence does not directly threaten people lives, it is a condition that can bringserious implications. Recent publications have shown significant improvement in clinical and quality of lifeof incontinent women submitted to conservative treatment. Therefore, this study aimed to evaluate the effectof physiotherapeutic treatment on the quality of life of a woman with urinary incontinence. Presentation ofthe case: a case study of a woman with stress urinary incontinence. Initially, a careful anamnesis and aphysical examination were undertaken to collect data such as past medical history, course of the disease, andthe muscle tonus of the pelvic floor. Then, the patient was asked to answer the International Consultation onIncontinence Questionnaire - Short Form. After 20 sessions of physiotherapy the same physical examinationand the same questionnaire were undertaken again. The initial and final scores and data previously collectedwere compared. Results: The patient had an increase in muscle tonus of the pelvic floor and increased thestrength degree of limbs, improved the clinical picture and their quality of life. Conclusion: This studyconcluded that physiotherapy based on kinesitherapy and electrotherapy transvaginal contributed to theimprovement of the clinical picture of stress urinary incontinence and, consequently, improves the quality oflife of the patients treated.