RESUMO
BACKGROUND: Synapses loss during aging is associated to neurophysiologic alterations that impair organism's health span, thus making the study and prevention of sensory decline relevant for healthy aging and welfare. Therefore the aim of this study was to obtain normative data related to the electrophysiological responses of the different neurosensory components in the visual, auditory and somatosensory pathways in healthy geriatric rhesus monkeys in captivity. METHODS: Twenty-four rhesus monkeys were divided in two groups: (i) Geriatric monkeys, 20-30 years of age, and (ii) Young monkeys, 7 years of age. Evoked potentials were obtained from the visual, auditory and somatosensory pathways. RESULTS: Regardless the sensory pathways evaluated, a significant delay in nerve conduction was observed in the geriatric group in comparison to the young group. CONCLUSIONS: Evoked potentials allowed identifying changes generated during aging in rhesus monkeys and normative data for this species were obtained.
Assuntos
Envelhecimento/fisiologia , Vias Auditivas/fisiologia , Macaca mulatta/fisiologia , Distúrbios Somatossensoriais/veterinária , Vias Visuais/fisiologia , Animais , Condutividade Elétrica , Potenciais Evocados Auditivos do Tronco Encefálico , Potenciais Somatossensoriais Evocados , Potenciais Evocados Visuais , Feminino , Masculino , Condução Nervosa/fisiologia , Distúrbios Somatossensoriais/fisiopatologia , Nervo Tibial/fisiologiaRESUMO
Summary The objective of the present study was to evaluate the viability of frozen embryos obtained from various private farmers in a culture medium for 4 h. Forty-seven embryos were used that had been previously graded as good and fair. These embryos were evaluated using stereoscopic microscopy by experienced clinicians prior to freezing. Embryos were divided in two groups: the non-cultured group, made up of six good quality embryos, and five fair; and the cultured group that consisted of 20 good quality embryos and 16 fair. Fifty-four per cent of the good quality embryos achieved a favourable development during culture whereas just 42% of embryos determined to be fair were observed to have adequate development. This evaluation was undertaken by serial photographs obtained at the onset of culture and 4 h later. This finding was corroborated by a more specific technique: terminal deoxynucleotide transferase dUTP nick end labelling-bromodeoxyuridine (TUNEL-BrdU). These results are indicative of the necessity of tight quality controls for commercially produced frozen embryos, as once thawed it is unlikely that clinicians will examine them to determine their physiological status prior to transfer.
Assuntos
Apoptose , Blastocisto/citologia , Proliferação de Células , Criopreservação , Meios de Cultura/química , Técnicas de Cultura Embrionária , Embrião de Mamíferos/citologia , Humanos , Marcação In Situ das Extremidades Cortadas , Microscopia EletrônicaRESUMO
BACKGROUND: We describe two clinical cases and examine the effects of piracetam on the brainstem auditory response in infantile female rhesus monkeys (Macaca mulatta). RESULTS: We found that the interwave intervals show a greater reduction in a 3-year-old rhesus monkey compared to a 1-year-old rhesus monkey. DISCUSSION: In this report, we discuss the significance of these observations.
Assuntos
Potenciais Evocados Auditivos do Tronco Encefálico/efeitos dos fármacos , Macaca mulatta , Nootrópicos/farmacologia , Piracetam/farmacologia , Anestesia , Animais , FemininoRESUMO
Se presentan los resultados de las pruebas de tamizaje para la deteccion de deficiencia de G6PD y hemoglobinas anormales efectuadas en muestras de sangre de 1321 y 1022 recien nacidos con y sin ictericia, respectivamente.Los resultados sugieren que la asociacion de ictericia neonatal con deficiencia de G6PD o hemoglobinas anormales no constituye un problema de salud publica en la poblacion mexicana