RESUMO
Slow-growing breeds are more resistant to Salmonella infection compared to fast-growing broilers. However, it is unclear whether that is associated with innate resistance or rather rely on differences in Salmonella-induced gut responses. We investigated the microbial composition and gene expression of nutrient transporters, mucin, and interleukin in the gut of a fast-growing (Cobb500) and a slow-growing naked neck (NN) chicken breeds challenged with Salmonella Enteritidis. Hatchlings were inoculated at two days of age using sterile broth (sham) or Salmonella Enteritidis (SE) and distributed according to a completely randomized design into four treatments: Cobb-sham; Cobb-SE; NN-sham; and NN-SE. Cecal SE counting and microbial composition by 16 S rRNA sequencing were determined at 24-, 96-, and 168-hours post-inoculation (hpi). Gene expression of amino acid (Asct1) and peptide transporters (PepT1), glucose transporters (Sglt1, Glut2 and Glut5) and mucin (Muc2) in the jejunum and expression of interleukins (IL1 beta, IL8, IL17 and IL22) in the cecum was assessed by qPCR at 24 and 168 hpi. NN birds were colonized by SE just as Cobb birds but showed innate upregulation of Muc2, IL8 and IL17 in comparison to Cobb. While nutrient transporter mRNA expression was impaired in SE-challenged Cobb birds, the opposite was observed in NN. There were no differences in microbial diversity at different sampling times for Cobb-SE, whereas the other groups had higher diversity and lower dominance at 24 hpi compared with 96 hpi and 168 hpi. NN birds apparently develop earlier gut microbial stability, have higher basal level of mucin gene expression as well as differential nutrient transporter and interleukin gene expression in the presence of SE which might mitigate the effects of SE infection compared to Cobb birds.
Assuntos
Galinhas , Microbioma Gastrointestinal , Interleucinas , Mucinas , Doenças das Aves Domésticas , Salmonelose Animal , Salmonella enteritidis , Animais , Galinhas/microbiologia , Salmonella enteritidis/genética , Salmonella enteritidis/crescimento & desenvolvimento , Doenças das Aves Domésticas/microbiologia , Doenças das Aves Domésticas/metabolismo , Salmonelose Animal/microbiologia , Mucinas/metabolismo , Mucinas/genética , Interleucinas/genética , Interleucinas/metabolismo , Ceco/microbiologia , Ceco/metabolismo , RNA Ribossômico 16S/genética , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/metabolismoRESUMO
This study evaluated the effect of dietary supplementation with glutamine and glutamic acid (Gln+Glu) on performance, intestinal morphometry, and carcass characteristics of broiler quails. Eight hundred birds were used, distributed in an entirely randomized design with 20 birds per experimental unit, and given five treatments (0.0; 0.2; 0.4; 0.6; and 0.8% Gln+Glu supplementation) with eight replicates. At 1-21 days of age, lower (P < 0.05) feed intake at 0.6 and 0.8% Gln+Glu supplementation and lower weight gain at 0.8% Gln+Glu supplementation compared to the control treatment were observed. By regression analysis, excluding the control treatment, there was an increasing linear effect (P < 0.05) for feed intake at 22 to 42 days of age. For intestinal morphometry, Gln+Glu supplementation only favored the villus development of the ileum (P < 0.05), giving it greater height at 0.2, 0.6, and 0.8% supplementation. Carcass characteristics, cuts, and edible viscera of the birds at 42 days were not affected (P > 0.05) by Gln+Glu supplementation levels. Thus, the glutamine and glutamic acid supplementation affected the performance and intestinal morphology of 21-d-old quails, decreasing feed intake and weight gain associated with the improvement of ileum morphology; conversely, performance and carcass characteristics at 42 days were not affected by amino acid supplementation.
Objetivou-se avaliar o efeito da suplementação dietética de glutamina e ácido glutâmico (Gln+Glu) sobre o desempenho, a morfometria intestinal e as características de carcaça de codornas de corte. Foram utilizadas 800 aves, distribuídas em delineamento inteiramente casualizado, com cinco tratamentos (0,0; 0,2; 0,4; 0,6 e 0,8% de suplementação de Gln+Glu) e oito repetições com 20 aves por unidade experimental. Na fase de um a 21 dias, constatou-se menor (P < 0,05) consumo de ração aos níveis de 0,6 e 0,8% de Gln+Glu e menor ganho de peso ao nível 0,8% de Gln+Glu em comparação ao tratamento controle; e, pela análise de regressão, excluindo-se o tratamento controle, houve efeito linear crescente (P < 0,05) para consumo de ração na fase 22 a 42 dias de idade. Para morfometria intestinal, a suplementação de Gln+Glu apenas favoreceu o desenvolvimento vilos do íleo (P < 0,05), conferindo-lhe maior altura aos níveis de 0,2; 0,6 e 0,8% de suplementação. As características de carcaça, cortes e vísceras comestíveis das aves aos 42 dias não foram afetadas (P > 0,05) pelos níveis de suplementação de Gln+Glu. Assim, a suplementação de glutamina e ácido glutâmico influenciou o desempenho e a morfometria intestinal de codornas de corte aos 21 dias de idade, promovendo redução do consumo de ração e do ganho, associado ao aumento morfométrico do íleo; por outro lado, o desempenho das aves e as suas características de carcaça aos 42 dias não foram afetados pela suplementação dos aminoácidos.
Assuntos
Animais , Ácido Glutâmico , Suplementos Nutricionais , Coturnix/crescimento & desenvolvimento , Dieta/veterinária , GlutaminaRESUMO
Oxidative stress significantly compromises the production efficiency of laying hens. It has been reported in literature that selenium (Se) in poultry diets has a positive effect on mitigating these effects. This study has been carried out to evaluate the effects of Se supplementation in feeds, from either an inorganic or a hydroxy-selenomethionine (OH-SeMet) source, on the performance and physiological traits of 50- to 70-wk-old Dekalb Brown laying hens under heat stress, and on their egg quality after different storage durations. The treatments consisted in supplementing 0.3 ppm of Se as sodium selenite (SS; 45%-0.7g/ton) or OH-SeMet (2%-15g/ton) in twelve 16-bird replicates. Supplementation with OH-SeMet resulted in a better performance of the laying hens than with SS: -5% feed conversion ratio and +3.6% of egg mass. A reduction in egg quality was observed with prolonged egg storage, which was mitigated with the use of OH-SeMet in laying hen diets. The use of OH-SeMet increased the antioxidant capacity of the birds, which showed higher glutathione peroxidase levels in the blood, kidneys, liver, and intestinal mucosa, in addition to a higher Se content in the eggs and a greater bone resistance. Thus, supplementing feeds with 0.3 ppm of OH-SeMet to 50- to 70-wk-old semi-heavy laying hens enhances their antioxidant capacity and leads to a higher egg quality and productivity than SS supplementation.
Assuntos
Selênio , Selenometionina , Animais , Feminino , Selenometionina/farmacologia , Antioxidantes , Galinhas , Óvulo , Selênio/farmacologia , Dieta/veterinária , Resposta ao Choque Térmico , Ração Animal/análise , Suplementos NutricionaisRESUMO
Salmonella Heidelberg (SH) is responsible for economic losses in poultry farming and food infections in humans and is a serious public health problem. Recently, there has been an increase in the frequency of isolation of this serotype in batches of broilers raised in Brazil. It is necessary to find new ways to help control this pathogen. The present study aimed to evaluate the effect of diets containing the compound Original XPC, which is a prebiotic-like fermented compound (PFC), and/or Sangrovit, which is a sanguinarine-based phytobiotic (SAN), on SH infection in broiler chicks. For this purpose, SH colonization in the cecum and its invasion into the spleen and liver were evaluated, as were the histopathological changes caused in these organs. The lowest cecal SH counts were observed in birds that ingested SAN, followed by those fed PFC (P < 0.05), with no added effect when the two bioproducts were used together (SAN + PFC). The mean SH and liver spleen counts did not differ between groups (P > 0.05). In general, birds from all groups challenged with SH showed similar macroscopic changes, such as hemorrhagic areas, hepatomegaly, and splenomegaly, such changes being more intense in the infected control group. The microscopic changes observed in the liver included hepatocyte congestion, heterophil infiltration in the sinusoid capillaries, areas of necrosis, and mononuclear inflammation. In the cecum, heterophilic infiltrate and thickening of the lamina propria were observed. In the ileum, the most common changes were congestion and thickening of the lamina propria and atrophy of the villi and crypts. The microscopic changes were less intense in the supplemented birds than the infected control group, and those supplemented with SAN developed the least changes. As ideal conditions for histomorphometric parameters of the ileum, the villus:crypt ratio in birds should be high, the villi should be long, and the crypts should be shallow. In the present study, higher mean heights and villus areas were observed in uninfected control and SAN group birds, and the crypt depth was lower in birds in the negative control group. The lowest villus:crypt ratio was observed in the birds of the infected control group. Although additional studies are needed, the preliminary results of the current investigation indicated that the addition of bioproducts, especially SAN, to the diet of birds helped to control SH infection, reducing its count in the cecum and improving overall and intestinal health.
Assuntos
Doenças das Aves Domésticas , Salmonelose Animal , Humanos , Animais , Galinhas/microbiologia , Dieta/veterinária , Salmonella , Salmonelose Animal/microbiologia , Ração Animal/análise , Doenças das Aves Domésticas/microbiologiaRESUMO
The increasing number of studies reporting the presence of Salmonella in environmental water sources suggests that it is beyond incidental findings originated from sparse fecal contamination events. However, there is no consensus on the occurrence of Salmonella as its relative serovar representation across non-recycled water sources. We conducted a meta-analysis of proportions by fitting a random-effects model using the restricted maximum-likelihood estimator to obtain the weighted average proportion and between-study variance associated with the occurrence of Salmonella in water sources. Moreover, meta-regression and non-parametric supervised machine learning method were performed to predict the effect of moderators on the frequency of Salmonella in non-recycled water sources. Three sequential steps (identification of information sources, screening and eligibility) were performed to obtain a preliminary selection from identified abstracts and article titles. Questions related to the frequency of Salmonella in aquatic environments, as well as putative differences in the relative frequencies of the reported Salmonella serovars and the role of potential variable moderators (sample source, country, and sample volume) were formulated according to the population, intervention, comparison, and outcome method (PICO). The results were reported according to the Preferred Reporting Items for Systematic Review and Meta-Analyzes statement (PRISMA). A total of 26 eligible papers reporting 148 different Salmonella serovars were retrieved. According to our model, the Salmonella frequency in non-recycled water sources was 0.19 [CI: 0.14; 0.25]. The source of water was identified as the most import variable affecting the frequency of Salmonella, estimated as 0.31 and 0.17% for surface and groundwater, respectively. There was a higher frequency of Salmonella in countries with lower human development index (HDI). Small volume samples of surface water resulted in lower detectable Salmonella frequencies both in high and low HDI regions. Relative frequencies of the 148 serovars were significantly affected only by HDI and volume. Considering that serovars representation can also be affected by water sample volume, efforts toward the standardization of water samplings for monitoring purposes should be considered. Further approaches such as metagenomics could provide more comprehensive insights about the microbial ecology of fresh water and its importance for the quality and safety of agricultural products.
RESUMO
The effect of in ovo threonine (Thr) supplementation on the ileal expression of glucose, peptide and amino acid transporters was assessed in Salmonella Enteritidis-challenged broiler chicks. At 17.5 days of incubation, fertile eggs were supplemented in the amniotic fluid with sterile saline or 3.5% threonine. Hatchlings were individually weighed, and Salmonella Enteritidis negative status was confirmed. At 2 days of age, half of the birds of each group were inoculated with sterile nutrient broth or Salmonella Enteritidis inoculum. Relative expression of sodium-dependent glucose transporter 1 (SGLT1), glucose transporter 2 (GLUT2), di- and tri-peptide transporter 1 (PepT1) and alanine, serine, cysteine, threonine transporter (ASCT1) was assessed at hatch, 2 and 9 days of age, i.e., before inoculation and 7 days post-inoculation (dpi). At 9 days of age (7dpi), threonine increased SGLT1 and GLUT2 expression, whereas GLUT2 expression decreased in Salmonella-challenged birds. There was a significant interaction between threonine and Salmonella for PepT1 and ASCT1. Threonine increased PepT1 expression only in non-challenged birds. In addition, in ovo supplementation increased expression of ASCT1 regardless of post-hatch inoculation; Salmonella inoculation resulted in decreased expression of ASCT1 only in supplemented birds. The results suggest that while intra-amniotic threonine administration in broiler embryos increases the expression of genes related to the absorption of monosaccharides and amino acids, Salmonella challenge may negatively affect the expression of protein related transporters in the ileum of broilers.
Assuntos
Doenças das Aves Domésticas , Salmonella enteritidis , Animais , Galinhas/metabolismo , Suplementos Nutricionais , Expressão Gênica , Íleo/metabolismo , Nutrientes , Óvulo , Doenças das Aves Domésticas/metabolismo , Treonina/farmacologiaRESUMO
Antimicrobial resistance (AMR) remains a major global public health crisis. The food animal industry will face escalating challenges to increase productivity while minimizing AMR, since the global demand for animal protein has been continuously increasing and food animals play a key role in the global food supply, particularly broiler chickens. As chicken products are sources of low-cost, high-quality protein, poultry production is an important economic driver for livelihood and survival in developed and developing regions. The globalization of the food supply, markedly in the poultry industry, is aligned to the globalization of the whole modern society, with an unprecedented exchange of goods and services, and transit of human populations among regions and countries. Considering the increasing threat posed by AMR, human civilization is faced with a complex, multifaceted problem compromising its future. Actions to mitigate antimicrobial resistance are needed in all sectors of the society at the human, animal, and environmental levels. This review discusses the problems associated with antimicrobial resistance in the globalized food chain, using the poultry sector as a model. We cover critical aspects of the emergence and dissemination of antimicrobial resistance in the poultry industry and their implications to public health in a global perspective. Finally, we provide current insights using the multidisciplinary One Health approach to mitigate AMR at the human-animal-environment interface.
Assuntos
Saúde Única , Aves Domésticas , Animais , Antibacterianos/farmacologia , Galinhas/microbiologia , Farmacorresistência Bacteriana , Cadeia Alimentar , Humanos , Aves Domésticas/microbiologiaRESUMO
Aim: Genomic analysis of a methicillin-resistant Staphylococcus aureus (MRSA) strain cultured from a non-migratory seabird at Fernando de Noronha Archipelago (Brazilian oceanic islands) was carried out to investigate the potential origin of MRSA genetic determinants in an ecological setting with minimal or absent antimicrobial selective pressure, and minimal interaction with humans and domestic animals. Results: The study determined mecA gene homology and the phylogenetic relatedness with mecA described in Staphylococcus sciuri, which was the major Staphylococcus spp. cultured from the birds. Our findings corroborate in silico assumptions that the mecA gene in MRSA strains clinically relevant for humans and animals originates from S. sciuri ancestors. Conclusion: Coagulase-negative staphylococci seem to be natural reservoirs of methicillin-resistant genes to S. aureus, even in environments with very low antimicrobial selection pressure.
Assuntos
Proteínas de Bactérias/genética , Aves/microbiologia , Staphylococcus aureus Resistente à Meticilina/genética , Proteínas de Ligação às Penicilinas/genética , Staphylococcus/genética , Animais , Brasil , Ilhas , Sequenciamento Completo do GenomaRESUMO
The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (blaKPC) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby-Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, blaKPC-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only blaKPC was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.
Assuntos
Proteínas de Bactérias/genética , Escherichia coli , Psittaciformes/microbiologia , beta-Lactamases/genética , Animais , Antibacterianos/farmacologia , Brasil/epidemiologia , Crime , Farmacorresistência Bacteriana Múltipla , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Klebsiella pneumoniae/enzimologia , Klebsiella pneumoniae/genética , Testes de Sensibilidade MicrobianaRESUMO
The intestinal physiology and mechanisms involved in nutrient transport are not well established in quails (Coturnix coturnix japonica). The present study assessed the growth performance, morphological development, duodenal density and the expression of Sglt1 and Glut2 of female Japanese quails from 1 to 49 days of age. The three small intestine segments were sampled weekly from 1 to 49 days of age to evaluate villus height, crypt depth and villus: crypt ratio, and goblet cell counts. Scanning electronic microscopy was used to determine duodenal villus density, and real-time polymerase chain reaction (qPCR) was used to study the sodium/glucose cotransporter-1 Sglt1 and glucose transporter Glut2 in the jejunum. Villus height and crypt depth in the duodenum, jejunum and ileum increased with age until 42 and 49 days of age (P < 0.001), and regression analysis evidenced a quadratic effect (P < 0.0001), indicating increasing values to a maximum and then a decrease afterwards. Goblet cell counts increased (P < 0.001) in duodenum, jejunum and ileum from 1 to 42 days, decreasing at 49 days, which was also corroborated by the regression analysis. Villus density in the duodenum was greater in the first week, decreased with age and increased again at 42 days, probably due to the proximity with egg production onset. The expression of Sglt1 and Glut2 mRNA in the jejunum varied with age. In conclusion, the intestinal mucosa of female Japanese quail developed morphologically until 42days and functionally until earlier ages, indicating an adaptation to the exogenous diet during the first weeks of life.
Assuntos
Proteínas Aviárias/genética , Coturnix , Transportador de Glucose Tipo 2/genética , Mucosa Intestinal/metabolismo , Transportador 1 de Glucose-Sódio/genética , Animais , Coturnix/anatomia & histologia , Coturnix/genética , Coturnix/crescimento & desenvolvimento , Coturnix/metabolismo , Feminino , Mucosa Intestinal/ultraestrutura , Microscopia Eletrônica de Varredura , RNA Mensageiro/metabolismoRESUMO
Todos os organismos vivos, tanto animais como vegetais, apresentam quantidades variáveis de minerais, que são necessários para manter seu metabolismo fisiológico, sendo esses nutrientes de grande importância para o desenvolvimento das espécies. Para poedeiras, o cálcio e o fósforo são minerais chaves para o crescimento e formação do ovo, e sua disponibilidade é mais crucial durante o período de postura, pois os dois estão envolvidos no processo de formação do ovo. Apesar da importância bioquímica, fisiológica e nutricional destes minerais pouco se conhece a respeito do seu processo de transporte intestinal. Portanto, considerando a importância do cálcio e fósforo na nutrição de poedeiras e a necessidade do desenvolvimento de estratégias que possibilitem seu melhor aproveitamento, objetivou-se com esta revisão descrever o funcionamento dos transportadores de cálcio e fósforo para aves de postura, bem como, os fatores nutricionais que interferem sobre os mesmos.(AU)
All living organisms, both animals and plants, have varying amounts of minerals, which are necessary to keep its physiological metabolism of these nutrients, are important for the development of species. For laying hens, calcium and phosphorus are key minerals for growth and egg formation, and its availability is more crucial during the laying period, because both are involved in egg formation process. Despite the biochemical, physiological and nutritional importance of these minerals little is known about the process of intestinal transport. Therefore, considering the importance of calcium and phosphorus in the nutrition of hens and the need to develop strategies that enable their better utilization, aimed with this review describe the operation of carriers calcium and phosphorus for laying hens, as well as the nutritional factors that interfere with about the same.(AU)
Assuntos
Animais , Cálcio , Fósforo , Minerais na Dieta , Ração Animal , Galinhas , Fenômenos Fisiológicos da Nutrição AnimalRESUMO
Todos os organismos vivos, tanto animais como vegetais, apresentam quantidades variáveis de minerais, que são necessários para manter seu metabolismo fisiológico, sendo esses nutrientes de grande importância para o desenvolvimento das espécies. Para poedeiras, o cálcio e o fósforo são minerais chaves para o crescimento e formação do ovo, e sua disponibilidade é mais crucial durante o período de postura, pois os dois estão envolvidos no processo de formação do ovo. Apesar da importância bioquímica, fisiológica e nutricional destes minerais pouco se conhece a respeito do seu processo de transporte intestinal. Portanto, considerando a importância do cálcio e fósforo na nutrição de poedeiras e a necessidade do desenvolvimento de estratégias que possibilitem seu melhor aproveitamento, objetivou-se com esta revisão descrever o funcionamento dos transportadores de cálcio e fósforo para aves de postura, bem como, os fatores nutricionais que interferem sobre os mesmos.
All living organisms, both animals and plants, have varying amounts of minerals, which are necessary to keep its physiological metabolism of these nutrients, are important for the development of species. For laying hens, calcium and phosphorus are key minerals for growth and egg formation, and its availability is more crucial during the laying period, because both are involved in egg formation process. Despite the biochemical, physiological and nutritional importance of these minerals little is known about the process of intestinal transport. Therefore, considering the importance of calcium and phosphorus in the nutrition of hens and the need to develop strategies that enable their better utilization, aimed with this review describe the operation of carriers calcium and phosphorus for laying hens, as well as the nutritional factors that interfere with about the same.
Assuntos
Animais , Cálcio , Fósforo , Galinhas , Minerais na Dieta , Ração Animal , Fenômenos Fisiológicos da Nutrição AnimalRESUMO
This study assessed the effect of both embryonic thermal manipulation and dietary threonine level on the response of broilers inoculated with Salmonella Enteritidis, considering bacterial counts in the cecal contents, intestinal morphology, mucin and heat shock protein 70 gene expression, body weight and weight gain. Thermal manipulation was used from 11 days of incubation until hatch, defining three treatments: standard (37.7°C), continuous high temperature (38.7°C) and continuous low temperature (36.7°C). After hatch, chicks were distributed according to a 3x2+1 factorial arrangement (three temperatures and two threonine levels and one sham-inoculated control). At two days of age, all chicks were inoculated with Salmonella Enteritidis, except for the sham-inoculated control group. There was no interaction between the factors on any analyses. High temperature during incubation was able to reduce colonization by Salmonella Enteritidis in the first days, reducing both Salmonella counts and the number of positive birds. It also increased mucin expression and decreased Hsp70 expression compared with other inoculated groups. High temperature during incubation and high threonine level act independently to reduce the negative effects associated to Salmonella Enteritidis infection on intestinal morphology and performance, with results similar to sham-inoculated birds. The findings open new perspectives for practical strategies towards the pre-harvest Salmonella control in the poultry industry.
Assuntos
Galinhas/imunologia , Dieta/veterinária , Íleo/imunologia , Doenças das Aves Domésticas/imunologia , Salmonelose Animal/imunologia , Treonina/administração & dosagem , Animais , Peso Corporal/efeitos dos fármacos , Peso Corporal/imunologia , Embrião de Galinha , Galinhas/genética , Galinhas/crescimento & desenvolvimento , Galinhas/microbiologia , Contagem de Colônia Microbiana , Regulação da Expressão Gênica , Proteínas de Choque Térmico HSP70/genética , Proteínas de Choque Térmico HSP70/imunologia , Íleo/metabolismo , Íleo/microbiologia , Mucina-2/genética , Mucina-2/imunologia , Doenças das Aves Domésticas/dietoterapia , Doenças das Aves Domésticas/genética , Doenças das Aves Domésticas/microbiologia , Salmonelose Animal/dietoterapia , Salmonelose Animal/genética , Salmonelose Animal/microbiologia , Salmonella enteritidis/imunologia , Temperatura , Aumento de Peso/efeitos dos fármacos , Aumento de Peso/imunologiaRESUMO
With the objective of evaluating the effect of reducing crude protein (CP) and L-glutamic acid supplementation, Dekalb Brown laying hens with 34 weeks of age. The birds were distributed in six treatments in a completely randomized design, eight replications of eight birds. The control diet contained 17% CP. The second and third treatments had CP reduced by 1.5 and 3.0 percentage points (pp) of CP in relation to the control diet, respectively. In the fourth treatment, was added the same amount of glutamate of the control diet (2.982%), and the last two treatments was added 1 and 2 pp of glutamate in relation to control diet (3.982 and 4.98%), respectively. The treatment with 15.17% CP + 0.341% L-glutamic acid (T4) promoted higher egg production, and the control diet showed greater egg weight than others treatments. There was no significant effect on the variables on quality of eggs. The T4 presented values of excreted nitrogen similar to the others treatments, with lower concentration of uric acid than the control diet and lower cost in the production. In conclusion, it is recommended the treatment with 15.17% CP + 0.341% L-glutamic acid for Brown laying hens with 34 weeks of age.(AU)
Com o objetivo de avaliar o efeito da redução da proteína bruta (PB) e suplementação de L-glutamato em poedeiras da linhagem Dekalb Brown com 34 semanas de idade, as aves foram distribuídas em seis tratamentos em um delineamento experimental inteiramente casualizado com oito repetições. A dieta controle continha 17% PB. Os segundo e terceiro tratamentos tinham redução de 1,5 e 3,0 pontos percentuais (pp) de PB em relação à dieta controle, respectivamente. No quarto tratamento, foi adicionada a mesma quantidade de glutamato da dieta controle (2,982%), e nos dois últimos tratamentos foram adicionados 1 e 2 pp de glutamato em relação à dieta controle (3,982 e 4,98%), respectivamente. O tratamento com 15,17% CP + 0,341% L-glutâmico (T4) promoveu maior produção de ovos, e a ração controle apresentou maior peso de ovos do que os outros tratamentos. Não houve efeito significativo sobre as variáveis relativas à qualidade dos ovos. O T4 apresentou valores de nitrogênio excretado semelhante aos demais tratamentos, com menor concentração de ácido úrico que a dieta controle e menor custo na produção. Em conclusão, recomenda-se o tratamento com 15,17% PB + 0,341% de ácido L-glutâmico para poedeiras semipesadas com 34 semanas de idade.(AU)
Assuntos
Animais , Galinhas/anatomia & histologia , Galinhas/metabolismo , Ração Animal/análise , Ração Animal , Ácido Glutâmico/administração & dosagem , /síntese químicaRESUMO
With the objective of evaluating the effect of reducing crude protein (CP) and L-glutamic acid supplementation, Dekalb Brown laying hens with 34 weeks of age. The birds were distributed in six treatments in a completely randomized design, eight replications of eight birds. The control diet contained 17% CP. The second and third treatments had CP reduced by 1.5 and 3.0 percentage points (pp) of CP in relation to the control diet, respectively. In the fourth treatment, was added the same amount of glutamate of the control diet (2.982%), and the last two treatments was added 1 and 2 pp of glutamate in relation to control diet (3.982 and 4.98%), respectively. The treatment with 15.17% CP + 0.341% L-glutamic acid (T4) promoted higher egg production, and the control diet showed greater egg weight than others treatments. There was no significant effect on the variables on quality of eggs. The T4 presented values of excreted nitrogen similar to the others treatments, with lower concentration of uric acid than the control diet and lower cost in the production. In conclusion, it is recommended the treatment with 15.17% CP + 0.341% L-glutamic acid for Brown laying hens with 34 weeks of age.
Com o objetivo de avaliar o efeito da redução da proteína bruta (PB) e suplementação de L-glutamato em poedeiras da linhagem Dekalb Brown com 34 semanas de idade, as aves foram distribuídas em seis tratamentos em um delineamento experimental inteiramente casualizado com oito repetições. A dieta controle continha 17% PB. Os segundo e terceiro tratamentos tinham redução de 1,5 e 3,0 pontos percentuais (pp) de PB em relação à dieta controle, respectivamente. No quarto tratamento, foi adicionada a mesma quantidade de glutamato da dieta controle (2,982%), e nos dois últimos tratamentos foram adicionados 1 e 2 pp de glutamato em relação à dieta controle (3,982 e 4,98%), respectivamente. O tratamento com 15,17% CP + 0,341% L-glutâmico (T4) promoveu maior produção de ovos, e a ração controle apresentou maior peso de ovos do que os outros tratamentos. Não houve efeito significativo sobre as variáveis relativas à qualidade dos ovos. O T4 apresentou valores de nitrogênio excretado semelhante aos demais tratamentos, com menor concentração de ácido úrico que a dieta controle e menor custo na produção. Em conclusão, recomenda-se o tratamento com 15,17% PB + 0,341% de ácido L-glutâmico para poedeiras semipesadas com 34 semanas de idade.
Assuntos
Animais , Galinhas/anatomia & histologia , Galinhas/metabolismo , Ração Animal , Ração Animal/análise , Ácido Glutâmico/administração & dosagemRESUMO
This study evaluated the expression of heat shock protein 70 kD (hsp70) in broiler chicken embryos subjected to cold (Experiment I) or high incubation temperature (Experiment II). In each experiment, fertile eggs were distributed in three incubators kept at 37.8 degrees C. At day 13 (D13), D16, and D19 of incubation, the embryos were subjected to acute cold (32 degrees C) or heat (40 degrees C) for 4-6 hr. Immediately after cold or heat exposure, samples from the liver, heart, breast muscle, brain, and lungs of 40 embryos were taken per age and treatment (control or stressed embryos). A tissue pool from 10 embryos was used as 1 replication. The levels of hsp70 in each tissue sample was quantified by Western blot analysis. The data were analyzed in a 3 x 2 factorial arrangement of treatments with four replications. hsp70 was detected in all embryo tissues, and the brain contained 2- to 5-times more hsp70 protein compared to the other tissues in either cold or heat stressed embryos. hsp70 increases were observed in the heart and breast muscle of cold stressed embryos at D16 and D19, respectively. Heat stressed embryos showed an increase of hsp70 in the heart at D13 and D19, and in the lung at D19 of incubation. Younger embryos had higher hsp70 synthesis than older embryos, irrespective of the type of thermal stressor. The results indicate that the expression of hsp70 in broiler chicken embryos is affected by cold and heat distress, and is tissue- and age-dependent.