RESUMO
El Síndrome de Burn-Out fue descripto en 1974 y se caracteriza por una progresiva pérdida de energía, hasta llegar al agotamiento, con aumento de los síntomas de ansiedad y depresión. Este Síndrome se presenta con agotamiento emocional, despersonalización y disminución del sentimiento de realización personal, acompañado de un sentimiento de pérdida de prestigio o reconocimiento personal. (AU)
Burn-Out Syndrome was described in 1974 and is characterized by a progressive loss of energy, to exhaustion, with increased symptoms of anxiety and depression. This syndrome occurs with emotional exhaustion, depersonalization and diminished feeling of personal fulfillment, accompanied by a feeling of loss of prestige or personal recognition. (AU)
Assuntos
Humanos , Médicos/psicologia , Esgotamento Profissional/epidemiologia , Esgotamento Psicológico/epidemiologia , Corpo Clínico Hospitalar/estatística & dados numéricosRESUMO
Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36-98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.
Assuntos
Doença de Chagas/diagnóstico , Transplante de Órgãos/efeitos adversos , Adolescente , Adulto , Idoso , Algoritmos , DNA de Cinetoplasto/genética , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Fatores de Tempo , Doadores de Tecidos , Trypanosoma cruzi/genética , Adulto JovemRESUMO
Diversity arrays technology (DArT) genomic libraries were developed from H. chilense accessions to support robust genotyping of this species and a novel crop comprising H. chilense genome (e.g., tritordeums). Over 11,000 DArT clones were obtained using two complexity reduction methods. A subset of 2,209 DArT markers was identified on the arrays containing these clones as polymorphic between parents and segregating in a population of 92 recombinant inbred lines (RIL) developed from the cross between H. chilense accessions H1 and H7. Using the segregation data a high-density map of 1,503 cM was constructed with average inter-bin density of 2.33 cM. A subset of DArT markers was also mapped physically using a set of wheat-H. chilense chromosome addition lines. It allowed the unambiguous assignment of linkage groups to chromosomes. Four segregation distortion regions (SDRs) were found on the chromosomes 2H(ch), 3H(ch) and 5H(ch) in agreement with previous findings in barley. The new map improves the genome coverage of previous H. chilense maps. H. chilense-derived DArT markers will enable further genetic studies in ongoing projects on hybrid wheat, seed carotenoid content improvement or tritordeum breeding program. Besides, the genetic map reported here will be very useful as the basis to develop comparative genomics studies with barley and model species.