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1.
J Assist Reprod Genet ; 38(8): 2007-2020, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33788133

RESUMO

PURPOSE: To study the use of in silica model to better understand and propose new markers of ovarian response to controlled ovarian stimulation before IVF. METHODS: A systematic review and in silica model using bioinformatics. After the selection of 103 papers from a systematic review process, we performed a GRADE qualification of all included papers for evidence-based quality evaluation. We included 57 genes in the silica model using a functional protein network interaction. Moreover, the construction of protein-protein interaction network was done importing these results to Cytoscape. Therefore, a cluster analysis using MCODE was done, which was exported to a plugin BINGO to determine Gene Ontology. A p value of < 0.05 was considered significant, using a Bonferroni correction test. RESULTS: In silica model was robust, presenting an ovulation-related gene network with 87 nodes (genes) and 348 edges (interactions between the genes). Related to the network centralities, the network has a betweenness mean value = 102.54; closeness mean = 0.007; and degree mean = 8.0. Moreover, the gene with a higher betweenness was PTPN1. Genes with the higher closeness were SRD5A1 and HSD17B3, and the gene with the lowest closeness was GDF9. Finally, the gene with a higher degree value was UBB; this gene participates in the regulation of TP53 activity pathway. CONCLUSIONS: This systematic review demonstrated that we cannot use any genetic marker before controlled ovarian stimulation for IVF. Moreover, in silica model is a useful tool for understanding and finding new markers for an IVF individualization. PROSPERO: CRD42020197185.


Assuntos
Fertilização in vitro , Ovário/metabolismo , Indução da Ovulação , Mapas de Interação de Proteínas/genética , Biologia Computacional , Simulação por Computador , Feminino , Redes Reguladoras de Genes/genética , Humanos , Ovário/crescimento & desenvolvimento , Prognóstico
2.
Reprod Biomed Online ; 21(4): 470-3, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20797901

RESUMO

This cross-sectional prospective study assessed follicular-fluid anti-Müllerian hormone (AMH) concentrations in infertile patients with mild/minimal endometriosis during natural IVF. Thirty-two women participated in the study. Patients were divided into two groups: tubal obstruction without endometriosis (control group) and minimal/mild endometriosis (study group). All patients underwent laparoscopy for assessment of infertility; at the same time, any foci of endometriosis found were cauterized. AMH concentration was measured in the follicular fluid of a single follicle when it achieved pre-ovulatory maturation by ultrasensitive enzyme-linked immunosorbent assay. Likewise, AMH, FSH and inhibin B content in serum was also measured. Age (30 ± 1.3 and 32 ± 0.8 years) and body mass index (22 ± 0.6 and 22 ± 0.5 kg/m(2)), day-3 antral follicle count (11.3 ± 1.7 and 10.7 ± 1.5), serum FSH concentrations (5.4 ± 0.6 and 5.0 ± 0.3 IU/ml) and follicular-fluid AMH concentrations (1.8 ± 0.3 and 1.5 ± 0.1 ng/ml, study and control group, respectively; mean difference 0.33, 95% CI -0.21 to 0.88) were similar in both groups. This study shows that infertile patients with minimal/mild endometriosis have a similar concentration of AMH in the follicular fluid after natural IVF as compared with control subjects.


Assuntos
Hormônio Antimülleriano/metabolismo , Líquido Folicular/química , Adulto , Hormônio Antimülleriano/sangue , Índice de Massa Corporal , Estudos Transversais , Endometriose , Feminino , Fertilização in vitro , Hormônio Foliculoestimulante/sangue , Humanos , Infertilidade Feminina , Inibinas/sangue
3.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;42(11): 1039-1043, Nov. 2009. ilus
Artigo em Inglês | LILACS | ID: lil-529104

RESUMO

Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 family. Endometriosis is strongly associated with sub-fertility, and affects about 15 percent of women of reproductive age. IL-18 may favor the progression of endometriosis. The objective of the present study was to determine the concentration of IL-18 in the serum and peritoneal fluid of infertile women with endometriosis. Forty infertile and 25 fertile women were screened in a teaching hospital. Thirty-four infertile patients with minimal or mild endometriosis and 22 fertile controls were enrolled in the study. The primary outcome was the estimate of IL-18 levels and the secondary outcome was the correlation between serum and peritoneal levels of IL-18. There were no differences between the two groups regarding age, body mass index and levels of peritoneal fluid IL-18 (mean ± SD): 290.85 ± 173.02 pg/mL for infertile women vs 374.21 ± 330.15 pg/mL for controls; or serum IL-18: 391.07 ± 119.71 pg/mL for infertile women vs 373.42 ± 129.11 pg/mL for controls. However, a positive association was found between serum and peritoneal IL-18 levels in patients with endometriosis: r = 0.794, P = 0.0001. All measurements were carried out at the same time by the Human IL-18 Immuno Assay ELISA kit (MBL Co. Ltd., Japan). The present study did not find evidence supporting the hypothesis that IL-18 levels are associated with infertility in women with minimal and mild endometriosis, although a positive correlation was detected in these women between peritoneal and serum levels of IL-18.


Assuntos
Adulto , Feminino , Humanos , Líquido Ascítico/química , Endometriose/metabolismo , Infertilidade Feminina/metabolismo , /análise , Estudos de Casos e Controles , Estudos Transversais , Endometriose/complicações , Infertilidade Feminina/etiologia , /sangue , Índice de Gravidade de Doença
4.
Braz J Med Biol Res ; 42(11): 1039-43, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19855902

RESUMO

Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 family. Endometriosis is strongly associated with sub-fertility, and affects about 15% of women of reproductive age. IL-18 may favor the progression of endometriosis. The objective of the present study was to determine the concentration of IL-18 in the serum and peritoneal fluid of infertile women with endometriosis. Forty infertile and 25 fertile women were screened in a teaching hospital. Thirty-four infertile patients with minimal or mild endometriosis and 22 fertile controls were enrolled in the study. The primary outcome was the estimate of IL-18 levels and the secondary outcome was the correlation between serum and peritoneal levels of IL-18. There were no differences between the two groups regarding age, body mass index and levels of peritoneal fluid IL-18 (mean +/- SD): 290.85 +/- 173.02 pg/mL for infertile women vs 374.21 +/- 330.15 pg/mL for controls; or serum IL-18: 391.07 +/- 119.71 pg/mL for infertile women vs 373.42 +/- 129.11 pg/mL for controls. However, a positive association was found between serum and peritoneal IL-18 levels in patients with endometriosis: r = 0.794, P = 0.0001. All measurements were carried out at the same time by the Human IL-18 Immuno Assay ELISA kit (MBL Co. Ltd., Japan). The present study did not find evidence supporting the hypothesis that IL-18 levels are associated with infertility in women with minimal and mild endometriosis, although a positive correlation was detected in these women between peritoneal and serum levels of IL-18.


Assuntos
Líquido Ascítico/química , Endometriose/metabolismo , Infertilidade Feminina/metabolismo , Interleucina-18/análise , Adulto , Estudos de Casos e Controles , Estudos Transversais , Endometriose/complicações , Feminino , Humanos , Infertilidade Feminina/etiologia , Interleucina-18/sangue , Índice de Gravidade de Doença
5.
Reprod Biomed Online ; 17(4): 564-8, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18854112

RESUMO

The aim of the present study was to evaluate the number of endometrial glandular openings, using previously reported software that provides an objective count, and to assess the variability of this parameter during the luteal phase in a population of women who had no hormonal abnormalities presenting with tubal infertility or male factor infertility. A cross-sectional study was performed comprising 561 patients selected for a diagnostic hysteroscopy for the investigation of infertility. Hysteroscopy was performed during the mid-secretory phase prior to the first IVF treatment cycle. A total of 561 image frames from all patients were analysed. All images were automatically selected by the software, which also evaluated the number of endometrial glandular openings. The mean +/- SD glandular opening count was 53.2 +/- 30 (range 4-158). The analysis of variation showed a significant difference (P = 0.001) among all video frames. In conclusion, endometrial glandular opening count, as measured by the method described, can be used in investigations during the luteal phase. Although a lack of pattern was observed in endometrial maturation, this feature should be explored further in this subgroup of patients.


Assuntos
Endométrio/diagnóstico por imagem , Endométrio/ultraestrutura , Fertilização in vitro , Infertilidade Feminina/diagnóstico por imagem , Estudos Transversais , Endométrio/fisiopatologia , Feminino , Humanos , Histeroscopia/métodos , Infertilidade Feminina/diagnóstico , Infertilidade Feminina/fisiopatologia , Fase Luteal/fisiologia , Variações Dependentes do Observador , Intensificação de Imagem Radiográfica/métodos
6.
J Assist Reprod Genet ; 24(8): 326-30, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17636446

RESUMO

PURPOSE: The purpose of the study was to compare the effectiveness of GnRH antagonist with luteal phase estradiol administration to GnRH agonist cycles, long protocol. METHODS: 55 IVF-ICSI patients received oestradiol in the luteal phase of the cycle, before a cycle with GnRH antagonist. Fifty-five patients submitted to IVF-ICSI with the use of agonist were allocated, age matched, as a control group (historical control). The primary outcome was the number of retrieved oocytes. RESULTS: Patients were similar in terms of clinical characteristics. No differences were found in the number of oocytes retrieved (study group, 8.1 +/- 4.7; control group, 7.4 +/- 4.5) or in oocyte quality. CONCLUSIONS: We clearly demonstrated that the effectiveness of GnRH antagonist when combined with luteal phase estradiol is comparable to GnRH agonist cycles.


Assuntos
Implantação do Embrião/fisiologia , Estradiol/administração & dosagem , Hormônio Liberador de Gonadotropina/agonistas , Hormônio Liberador de Gonadotropina/antagonistas & inibidores , Leuprolida/administração & dosagem , Fase Luteal/efeitos dos fármacos , Oócitos/efeitos dos fármacos , Indução da Ovulação , Adolescente , Adulto , Estudos de Casos e Controles , Gonadotropina Coriônica/farmacologia , Implantação do Embrião/efeitos dos fármacos , Feminino , Fertilização in vitro , Humanos , Fase Luteal/metabolismo , Gravidez , Injeções de Esperma Intracitoplásmicas
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