RESUMO
The pattern of growth, adhesion and protein synthesis in Vero cells submitted to nutritional stress conditions was investigated. The control cells presented a characteristic pattern, with monolayer growth, while the stressed cells presented multilayered growth, with aggregate or spheroid formation which detached on the flask surface and continued their growth in another region. In the soft agar assay, with reduced amount of nutrients, only the stressed cells presented growth, indicating physical and nutritional independence. A 44-kDa protein was observed in stressed cells and was absent in non-stressed cells. The adhesion index and fibronectin synthesis and distribution were altered in stressed cells. After confluence, control cells presented fibronectin accumulation in lateral cell-cell contact regions, while this fibronectin accumulation pattern was not observed in stressed cells. These alterations may be responsible for the multilayered growth and decreased adhesion index observed in stressed cells which were transformed by nutritional stress conditions.
Assuntos
Células Vero/citologia , Animais , Adesão Celular/fisiologia , Técnicas de Cultura de Células , Divisão Celular/fisiologia , Chlorocebus aethiops , Meios de Cultura/metabolismo , Fibronectinas/metabolismo , Imuno-Histoquímica , Fatores de Tempo , Células Tumorais CultivadasRESUMO
The Vero lineage, established from kidney cells of the green. African monkey, presented fibroblasts-like cells and growth in monolayers. Maintained in culture, the Vero cells presented behavioural and morphologic alterations, associated with cellular transformation. The morphological alterations were investigated using scanning and transmission electron microscopy. The study of proliferation and determination of the cellular doubling time was obtained from the growth curve. The initial population presented growth in a monolayer, while the altered cells grew in multilayers forming cellular aggregates, with flattened cells on the surface and globular cells in the inner region of the aggregate, together with extracellular matrix material. The cell surface of the altered population presented innumerable structures similar to little vesicles, microvilli and cytoplasmic prolongations. The cellular proliferation of both populations was very similar. Our results indicate that morphological and growth changes probably resulted from cellular transformation of the initial Vero cells. These transformed cells presented several characteristics associated with neoplastic growth, and can be used as a model for tumor cells studies in vitro.
Assuntos
Células Vero/patologia , Células Vero/ultraestrutura , Animais , Divisão Celular , Linhagem Celular Transformada , Chlorocebus aethiops , Microscopia Eletrônica de Transmissão e VarreduraRESUMO
Arising from a Vero cell line, an altered population was spontaneously obtained, which grew in multiple layers. Cytogenetic analyses revealed a higher polyploidy index (19%) than occurred in the initial population (5%). A modal chromosome number of 56 was found for the altered population, while the initial cell culture had only 54 chromosomes. Karyotypic studies showed that the extranumerary chromosomes were homologues of the 8th and the 27th pairs. The altered population probably resulted from a transformation of the initial line, and their transformed phenotypes may have been associated with the cytological differences.