RESUMO

Zika (ZIKV) and Chikungunya (CHIKV) viruses are mosquito-transmitted infections, or vector-borne pathogens, that emerged a few years ago. Reliable diagnostic tools for ZIKV and CHIKV-inexpensive, multiplexed, rapid, highly sensitive, and specific point-of-care (POC) systems-are vital for appropriate risk management and therapy. We recently studied a detection system with great success in Mexico (Villahermosa, state of Tabasco), working with human sera from patients infected with those viruses. The research conducted in Mexico validated the efficacy of a novel two-step rapid isothermal amplification technique (RAMP). This approach, which encompasses recombinase polymerase amplification (RPA) followed by loop-mediated isothermal amplification (LAMP), had been previously established in the lab using lab-derived Zika (ZIKV) and Chikungunya (CHIKV) viruses. Crucially, our findings confirmed that this technique is also effective when applied to human sera samples collected from locally infected individuals in Mexico.

Assuntos

Vírus Chikungunya , Técnicas de Amplificação de Ácido Nucleico , Infecção por Zika virus , Zika virus , Humanos , Zika virus/genética , Zika virus/isolamento & purificação , Técnicas de Amplificação de Ácido Nucleico/métodos , Vírus Chikungunya/genética , Vírus Chikungunya/isolamento & purificação , Infecção por Zika virus/diagnóstico , Infecção por Zika virus/virologia , Infecção por Zika virus/sangue , Febre de Chikungunya/diagnóstico , Febre de Chikungunya/virologia , Febre de Chikungunya/sangue , Técnicas de Diagnóstico Molecular/métodos , RNA Viral/genética , RNA Viral/sangue , México , Sensibilidade e Especificidade , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação

RESUMO

Humanitarian crises create opportunities for both in-person and remote aid. Durable, complex, and team-based care may leverage a telemedicine approach for comprehensive support within a conflict zone. Barriers and enablers are detailed, as is the need for mission expansion due to initial program success. Adapting a telemedicine program initially designed for critical care during the severe acute respiratory syndrome coronavirus 2 pandemic offers a solution to data transfer and data analysis issues. Staffing efforts and grouped elements of patient care detail the kinds of remote aid that are achievable. A multiprofessional team-based approach (clinical, administrative, nongovernmental organization, government) can provide comprehensive consultation addressing surgical planning, critical care management, infection and infection control management, and patient transfer for complex care. Operational and network security create parallel concerns relevant to avoid geolocation and network intrusion during consultation. Deliberate approaches to address cultural differences that influence relational dynamics are also essential for mission success.