RESUMO

In this study, two strains of Bdellovibrio were isolated from soil samples using the culture-dependent technique and two members of the family Enterobacteriaceae (Klebsiella sp. and Salmonella sp.) as prey. The Bdellovibrio strains were bacteriolytic, plaque-forming, and highly motile gram-negative bacteria. We identified and confirmed the Bdellovibrio strains using microscopy, PCR amplification, and sequencing of the 16S rRNA gene. They were observed to be different strains based on hit locus and prey range analyses. Here, the first report on Bdellovibrio strains isolated from soil in Mexico corroborates earlier report indicating that populations of Bdellovibrio found in soil are heterogeneous thereby the need to identify the various strains.

Assuntos

Bacteriólise/fisiologia , Bdellovibrio/isolamento & purificação , Agentes de Controle Biológico/metabolismo , Klebsiella/crescimento & desenvolvimento , Salmonella/crescimento & desenvolvimento , Sequência de Bases , Bdellovibrio/classificação , Bdellovibrio/genética , DNA Bacteriano/genética , México , Microscopia de Força Atômica , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Microbiologia do Solo