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BACKGROUND: A paucity of predictive models assessing risk factors for COVID-19 mortality that extend beyond age and gender in Latino population is evident in the current academic literature. OBJECTIVES: To determine the associated factors with mortality, in addition to age and sex during the first year of the pandemic. DESIGN: A case-control study with retrospective revision of clinical and paraclinical variables by systematic revision of clinical records was conducted. Multiple imputations by chained equation were implemented to account for missing variables. Classification and regression trees (CART) were estimated to evaluate the interaction of associated factors on admission and their role in predicting mortality during hospitalisation. No intervention was performed. SETTING: High-complexity centre above 2640 m above sea level (masl) in Colombia. PARTICIPANTS: A population sample of 564 patients admitted to the hospital with confirmed COVID-19 by PCR. Deceased patients (n=282) and a control group (n=282), matched by age, sex and month of admission, were included. MAIN OUTCOME MEASURE: Mortality during hospitalisation. MAIN RESULTS: After the imputation of datasets, CART analysis estimated 11 clinical profiles based on respiratory distress, haemoglobin, lactate dehydrogenase, partial pressure of oxygen to inspired partial pressure of oxygen ratio, chronic kidney disease, ferritin, creatinine and leucocytes on admission. The accuracy model for prediction was 80.4% (95% CI 71.8% to 87.3%), with an area under the curve of 78.8% (95% CI 69.63% to 87.93%). CONCLUSIONS: This study discloses new interactions between clinical and paraclinical features beyond age and sex influencing mortality in COVID-19 patients. Furthermore, the predictive model could offer new clues for the personalised management of this condition in clinical settings.
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COVID-19 , Humanos , Estudos de Casos e Controles , SARS-CoV-2 , Estudos Retrospectivos , Oxigênio , Mortalidade HospitalarRESUMO
B-Box-containing zinc finger transcription factors (BBX) are involved in light-mediated growth, affecting processes such as hypocotyl elongation in Arabidopsis thaliana. However, the molecular and hormonal framework that regulates plant growth through BBX proteins is incomplete. Here, we demonstrate that BBX21 inhibits the hypocotyl elongation through the brassinosteroid (BR) pathway. BBX21 reduces the sensitivity to 24-epiBL, a synthetic active BR, principally at very-low concentrations in simulated shade. The biosynthesis profile of BRs showed that two active BR -brassinolide (BL) and 28-homobrassinolide (28-homoBL)- and 8 of 11 intermediates can be repressed by BBX21 under white light (WL) or simulated shade. Furthermore, BBX21 represses the expression of CYTOCHROME P450 90B1 (DWF4/CYP90B1), BRASSINOSTEROID-6-OXIDASE 1 (BR6OX1, CYP85A1) and BR6OX2 (CYP85A2) genes involved in the BR biosynthesis in WL while specifically promoting DWF4 and PHYB ACTIVATION TAGGED SUPPRESSOR 1 (CYP2B1/BAS1) expression in WL supplemented with far-red (WL+FR), a treatment that simulates shade. In addition, BBX21 represses BR signalling genes such as PACLOBUTRAZOL RESISTANCE1 (PRE1), PRE3 and ARABIDOPSIS MYB-LIKE 2 (MYBL2), and auxin-related and expansin genes, such as INDOLE-3-ACETIC ACID INDUCIBLE 1 (IAA1), IAA4 and EXPANSIN 11 (EXP11) in short-term shade. By a genetic approach we found that BBX21 acts genetically upstream of BRASSINAZOLE-RESISTANT 1 (BZR1) for the promotion of DWF4 and BAS1 gene expression in shade. We propose that BBX21 integrates the BR homeostasis and shade-light signalling allowing the fine-tuning of hypocotyl elongation in Arabidopsis.
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OBJECTIVE: Rhupus is a rare disease that shares characteristics of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). While several studies have explored the clinical and immunological profiles of patients with rhupus, the underlying cause of the disease remains unknown due to its complex pathogenesis. The objective of this study was to investigate the role of tumour necrosis factor (TNF) in the production of inflammatory molecules by peripheral blood mononuclear cells (PBMCs) from patients with rhupus. METHODS: The study involved five healthy controls, seven patients with rhupus and seven patients with SLE. PBMCs were obtained from each participant and stimulated with recombinant human TNF for 24 hours. The levels of various molecules secreted by the cells, such as cytokines and chemokines, were measured using immunobead-based assays on xMAP technology. RESULTS: The production levels of some molecules were higher in TNF-stimulated PBMCs from patients with rhupus and SLE than in unstimulated cells. In addition, the levels of certain molecules, including gp130/sIL-6Rb, a proliferation-inducing ligand (APRIL), interferon-ß, matrix metalloproteinase-3 and interleukin (IL)-12, were higher in PBMCs from patients with rhupus even without TNF stimulation. Similarly, the levels of gp130/sIL-6Rb and APRIL were higher in TNF-stimulated PBMCs from patients with rhupus than in healthy controls. These results were further validated against patients with RA using enzyme-linked immunosorbent assay. CONCLUSIONS: These findings suggest that the spontaneous production of molecules by cells from patients with rhupus may contribute to the development of the disease, and that TNF may play a role in this process by regulating the secretion of gp130/sIL-6Rb and APRIL.
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Artrite Reumatoide , Lúpus Eritematoso Sistêmico , Humanos , Receptor gp130 de Citocina , Leucócitos Mononucleares , Membro 13 da Superfamília de Ligantes de Fatores de Necrose Tumoral , Fator de Necrose Tumoral alfa/metabolismo , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
Resumen Introducción: Los procedimientos dentales han sido asociados a bacteriemia y endocarditis infecciosa. Objetivo: Determinar la prevalencia de endocarditis infecciosa a partir de procedimientos odontológicos. Materiales y métodos: Se realizó un estudio descriptivo de tipo retrospectivo cuantitativo. Se incluyeron historias clínicas de pacientes con endocarditis infecciosa a los cuales se les registró identificación general, sexo, edad, estrato socioeconómico, reporte de procedimiento odontológico, tipo de procedimiento odontológico, endocarditis infecciosa no especificada y reporte de procedimiento médico-quirúrgico. Adicionalmente, se registró información sobre el tipo de procedimiento médico-quirúrgico o condición médica relacionada. Los datos fueron compilados en hoja de cálculo para su procesamiento en software estadístico (SPSS ver. 25). Resultados: De 154 casos de endocarditis infecciosa registrados, solo en uno (0.7%) se reportó procedimiento odontológico del tipo endodoncia previo a la hospitalización. La causa relacionada más comúnmente reportada fue cateterismo para hemodiálisis (37%) seguido de bacteriemia no específica (22%) y condición cardiovascular asociada a válvulas cardíacas y marcapasos (18.8%). En el 15.6% de los casos se reportó como endocarditis infecciosa no especificada. Los microorganismos más comúnmente aislados pertenecen al género Staphylococcus, seguido de Streptococcus. Conclusiones: La frecuencia de endocarditis bacteriana relacionada con procedimientos odontológicos fue menor del 1%. Los procedimientos médico-quirúrgicos siguen siendo la causa más común de endocarditis bacteriana.
Abstract Introduction: Dental procedures have been associated with bacteriemia and infective endocarditis. Objective: To determine the prevalence of infective endocarditis from dental procedures. Materials and methods: A descriptive, retrospective quantitative study was carried out. Records of infective endocarditis of which the general identification, sex, age, socioeconomic status, dental procedure report, type of dental procedure and non-specified infective endocarditis information was collected. Additionally, information was recorded on the type of medical/surgical procedure or medical condition that was associated. Categorical variables are presented as absolute and relative frequencies. The data were compiled in a spreadsheet for processing in statistical software (SPSS ver. 25). Results: Of the 154 cases of infective endocarditis registered, only 1 case (0.7%) reported an endodontic-type dental procedure prior to hospitalization. The most reported related cause was catheterization for hemodialysis (37%) followed by unspecified bacteriemia (22%) and cardiovascular condition associated with heart valves and pacemakers (18.8%). In 15.6% of the cases, it was reported as non-specific infective endocarditis. The most isolated microorganisms belonged to the genus Staphylococcus followed by Streptococcus. Conclusions: The frequency of bacterial endocarditis related to dental procedures was less than 1%. Medical-surgical procedures remain the most common cause of bacterial endocarditis.
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Cardiologia , EducaçãoRESUMO
RESUMO Objetivo: construir e validar cenários de simulação clínica para desenvolvimento da habilidade de comunicação em saúde de estudantes/profissionais no atendimento aos pacientes com hanseníase e seus contactantes. Método: desenvolveu-se pesquisa metodológica de novembro de 2020 a dezembro de 2021 em três etapas: construção dos cenários, validação e realização do teste piloto. A construção dos cenários se deu com base na literatura. Participaram da validação dez juízes com expertise em hanseníase e/ou simulação clínica, avaliando os cenários de forma remota por meio de formulários do Google Forms, pelo Índice de Validação de Conteúdo (IVC), no qual um cenário é validado se seu IVC for ≥ 0,80. Os dados foram analisados no software SPSS. O teste piloto foi dividido em: exposição de aula teórica, participação do cenário e debriefing. Participaram cinco estudantes e um profissional da área da saúde. Resultados: Foram elaborados três cenários: suspeição diagnóstica e classificação em hanseníase; vigilância dos contatos e informações sobre aplicação da vacina BCG; consulta na alta por cura, contendo, respectivamente, nove, oito e nove itens, todos com concordância satisfatória (IVC ≥ 0,90). Conclusão: considerou-se validados os cenários da pesquisa, estando disponíveis como novo material didático para fomentar o ensino na área da saúde.
RESUMEN Objetivo: construir y validar escenarios de simulación clínica para el desarrollo de la habilidad de comunicación en salud de estudiantes/profesionales en la atención a los pacientes con lepra y sus contactantes. Método: se desarrolló investigación metodológica de noviembre de 2020 a diciembre de 2021 en tres etapas: construcción de los escenarios, validación y realización de la prueba piloto. La construcción de los escenarios se dio con base en la literatura. En la validación participaron diez jueces con experiencia en lepra y/o simulación clínica, evaluando los escenarios de forma remota por medio de formularios de Google Forms, por el Índice de Validación de Contenido (IVC), en el cual un escenario es validado si su IVC es ≥ 0,80. Los datos fueron analizados en el software SPSS. La prueba piloto se dividió en: exposición de clase teórica, participación del escenario y debriefing. Participaron cinco estudiantes y un profesional de área de la salud. Resultados: fueron elaborados tres escenarios: sospecha diagnóstica y clasificación en lepra; vigilancia de los contactos e información sobre aplicación de la vacuna BCG; consulta en el alta por cura, conteniendo, respectivamente, nueve, ocho y nueve ítems, todos con concordancia satisfactoria (IVC ≥ 0,90). Conclusión: los escenarios de la investigación fueron considerados validados, estando disponibles como nuevo material didáctico para fomentar la enseñanza en el área de la salud.
ABSTRACT Objective: To construct and validate clinical simulation scenarios for the development of health communication skills of students/professionals in the care of leprosy patients and their contacts. Method: Methodological research carried out from November 2020 to December 2021 in three stages: construction of scenarios, validation and carrying out the pilot testing. The construction of the scenarios was based on the literature. A total of ten judges with expertise in leprosy and/or clinical simulation participated in the validation, evaluating the scenarios remotely through Google Forms, using the Content Validation Index (CVI), in which a scenario is validated if its CVI is ≥ 0.80. Data were analyzed using SPSS software. The pilot test was divided into: exposition of theoretical class, participation in the scenario and debriefing. Five students and one health professional participated in the study. Results: Three scenarios were elaborated: diagnostic suspicion and leprosy classification; surveillance of contacts and information on the application of the BCG vaccine; consultation at discharge due to cure containing, respectively, nine, eight and nine items, all with satisfactory agreement (CVI ≥ 0.90). Conclusion: the research scenarios were considered validated, being available as new didactic material to promote teaching in the health area.
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Humanos , Masculino , Feminino , Educação em Saúde , Pessoal de Saúde , Diagnóstico , Treinamento por SimulaçãoRESUMO
Under adverse conditions such as shade or elevated temperatures, cotyledon expansion is reduced and hypocotyl growth is promoted to optimize plant architecture. The mechanisms underlying the repression of cotyledon cell expansion remain unknown. Here, we report that the nuclear abundance of the BES1 transcription factor decreased in the cotyledons and increased in the hypocotyl in Arabidopsis thaliana under shade or warmth. Brassinosteroid levels did not follow the same trend. PIF4 and COP1 increased their nuclear abundance in both organs under shade or warmth. PIF4 directly bound the BES1 promoter to enhance its activity but indirectly reduced BES1 expression. COP1 physically interacted with the BES1 protein, promoting its proteasome degradation in the cotyledons. COP1 had the opposite effect in the hypocotyl, demonstrating organ-specific regulatory networks. Our work indicates that shade or warmth reduces BES1 activity by transcriptional and post-translational regulation to inhibit cotyledon cell expansion.
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Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Brassinosteroides/metabolismo , Proteínas de Ligação a DNA/metabolismo , Regulação da Expressão Gênica de Plantas , Hipocótilo/metabolismoRESUMO
BACKGROUND: Paraoxonase-1 (PON1), a glycoprotein associated with serum high-density lipoprotein (HDL), has a central role in metabolizing lipid peroxides, exhibiting antiatherogenic properties. The polymorphism p.Q192R has been previously associated with coronary artery disease (CAD) susceptibility and clopidogrel response. PURPOSE: We aimed at investigating the association of PON1 p.Q192R with CAD and clopidogrel response in Colombian population. PATIENTS AND METHODS: The study was conducted among 163 patients diagnosed with CAD and treated with clopidogrel. The allele frequencies for the PON1 192Q and 192R alleles were determined in cases and Latin-American controls obtained from the public database gnomAD (n = 17,711). Response to clopidogrel was determined by assessing the platelet function using the INNOVANCE PFA-200 System. We determined the association between PON1 p.Q192R polymorphism, increased susceptibility to CAD and high on-treatment platelet reactivity (HPR) by using odds ratio (OR) and 95% confidence interval (CI) on four genetic models. RESULTS: The allele frequencies for the PON1 192Q and 192R alleles were 0.60 and 0.40, respectively. The allele distribution was found to be statistically different from the control group and other ethnic groups. The allele 192R was positively associated with decreased susceptibility to CAD under a dominant model (OR, 0.58; 95% CI, 0.42-0.8; P < 0.01). We found no association between the polymorphism and HPR. CONCLUSION: We propose that PON1 p.Q192R is a potentially useful marker for CAD susceptibility in the Colombian population and lacks association with HPR under clopidogrel treatment.
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Arildialquilfosfatase , Doença da Artéria Coronariana , Arildialquilfosfatase/genética , Clopidogrel/uso terapêutico , Colômbia/epidemiologia , Doença da Artéria Coronariana/tratamento farmacológico , Doença da Artéria Coronariana/epidemiologia , Doença da Artéria Coronariana/genética , Genótipo , Humanos , Inibidores da Agregação Plaquetária/uso terapêuticoRESUMO
RESUMEN Fundamento: la identificación oportuna de estrategias y metas de aprendizaje en estudiantes es importante en el éxito de los propósitos del proceso enseñanza aprendizaje. Objetivo: describir las estrategias de aprendizaje y metas académicas en estudiantes matriculados de primero a sexto semestres, durante el período académico octubre 2016 - febrero 2017, en la Facultad de Ciencias de la Salud de la Universidad Nacional de Chimborazo. Método: se realizó un estudio observacional descriptivo, durante el período académico octubre 2016 - febrero 2017, en la Facultad de Ciencias de la Salud de la Universidad Nacional de Chimborazo, en el que se aplicaron los cuestionarios para la evaluación de metas académicas (CEMA) y de estrategias de trabajo autónomo (CETA), cuyos datos resultantes fueron analizados e interpretados utilizando métodos estadísticos de los niveles descriptivo e inferencial paramétrico. Resultados: se observó un predominio de la independencia entre la carrera que cursaban y las estrategias de aprendizaje; pero existió dependencia significativa (p=0,02<0,05) con respecto a las de conceptualización y muy significativa para las de participación (p=0,006<0,01). La media de los tipos de metas académicas estudiadas ubicó a casi todos en la categoría algunas veces (medias entre 3,11 y 3,28); a excepción de las orientadas al aprendizaje (3,59 de media) para un 41,78 %. Conclusiones: ninguna de las estrategias de aprendizaje predominó entre la población estudiada, prevaleció la categoría algunas veces en cada uno; sin embargo, entre las metas académicas predominaron aquellas orientadas al aprendizaje, las que se ubicaron mayoritariamente en el valor de escala muchas veces.
ABSTRACT Background: the timely identification of strategies and learning goals in students is important in the success of the purposes of the teaching-learning process. Objective: to describe the learning strategies and academic goals in students enrolled from first to sixth semesters, during the academic period October 2016 - February 2017, at the Faculty of Health Sciences of the National University of Chimborazo. Method: a descriptive observational study was carried out, during the academic period October 2016 - February 2017, at the Faculty of Health Sciences of the National University of Chimborazo, in which the questionnaires for the evaluation of academic goals (QEAG) were applied and autonomous work strategies (AWS), whose resulting data were analyzed and interpreted using statistical methods of the descriptive and inferential parametric levels. Results: a predominance of independence was observed between the career they were studying and the learning strategies; but there was a significant dependence (p = 0.02 <0.05) with respect to conceptualization and very significant for participation (p = 0.006 <0.01). The mean of the types of academic goals studied placed almost all of them in the category at times (means between 3.11 and 3.28); With the exception of those oriented to learning (3.59 on average) for 41.78%. Conclusions: none of the learning strategies predominated among the studied population, the category at times prevailed in each one; however, among the academic goals, those oriented to learning predominated, which were mostly located in the scale value many times.
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Educação de Graduação em Medicina , Educação Profissionalizante , Avaliação EducacionalRESUMO
RESUMEN La presentación inicial de los tumores cerebrales depende en gran medida de su localización y las estructuras que se encuentren adyacentes, y en algunos casos pueden tener un curso asintomático. Sin embargo, una de las causas poco frecuentes pero bien conocidas de hemorragias intracraneales espontáneas, es la presentación inicial de tumores cerebrales primarios y secundarios. En este artículo se presenta el caso de un paciente de 72 años que ingresó al servicio de urgencias con alteración del estado de conciencia y hemiparesia braquiocrural izquierda. Al examen físico presentó cifras tensionales elevadas, por lo que se realizó una tomografía de cráneo que puso en evidencia una lesión ocupante de espacio con efecto de masa y características que sugirió una hemorragia intratumoral, la cual requirió drenaje y resección del tumor extraaxial. Posteriormente, el resultado de la patología reveló un meningioma atípico grado II.
SUMMARY The initial presentation of brain tumors will depend especially on their location, adjacent structures or in some cases may have an asymptomatic course. However, one of the rare but well-known causes of spontaneous intracraneal hemorrhages is the initial presentation of primary and secondary brain tumors. This article presents the case of a 72-year-old patient who has entered to the emergency department with altered consciousness state and left brachio-crural hemiparesis, the patient in the vital signs has presented high-tension rates. The cranial CT has shown a space occupying lesion with mass effect and characteristics suggesting intratumoral hemorrhage that required drainage and resection of the extra-axial tumor. The result of the pathology revealed an atypical meningioma grade II.
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Mobilidade UrbanaRESUMO
Coumarin-hydroxamic acid derivatives 7a-k were herein designed with a dual purpose: as antiproliferative agents and fluorescent probes. The compounds were synthesized in moderate yields (30-87%) through a simple methodology, biological evaluation was carried out on prostate (PC3) and breast cancer (BT-474 and MDA-MB-231) cell lines to determine the effects on cell proliferation and gene expression. For compounds 7c, 7e, 7f, 7i and 7j the inhibition of cancer cell proliferation was similar to that found with the reference compound at a comparable concentration (10 µM), in addition, their molecular docking studies performed on histone deacetylases 1, 6 and 8 showed strong binding to the respective active sites. In most cases, antiproliferative activity was accompanied by greater levels of cyclin-dependent kinase inhibitor p21, downregulation of the p53 tumor suppressor gene, and regulation of cyclin D1 gene expression. We conclude that compounds 7c, 7e, 7f, 7i and 7j may be considered as potential anticancer agents, considering their antiproliferative properties, their effect on the regulation of the genes, as well as their capacity to dock to the active sites. The fluorescent properties of compound 7j and 7k suggest that they can provide further insight into the mechanism of action.
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Neoplasias da Mama , Proliferação de Células/efeitos dos fármacos , Cumarínicos , Corantes Fluorescentes , Inibidores de Histona Desacetilases , Ácidos Hidroxâmicos , Simulação de Acoplamento Molecular , Neoplasias da Próstata , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Cumarínicos/síntese química , Cumarínicos/química , Cumarínicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Corantes Fluorescentes/química , Corantes Fluorescentes/farmacologia , Inibidores de Histona Desacetilases/síntese química , Inibidores de Histona Desacetilases/química , Inibidores de Histona Desacetilases/farmacologia , Humanos , Ácidos Hidroxâmicos/química , Ácidos Hidroxâmicos/farmacologia , Masculino , Proteínas de Neoplasias/antagonistas & inibidores , Proteínas de Neoplasias/metabolismo , Células PC-3 , Neoplasias da Próstata/tratamento farmacológico , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Relação Estrutura-AtividadeRESUMO
The possible role of the social rank (R) (i.e., low-LSR, middle-MSR, or high-HSR) in anestrus goats exposed to a P4 + eCG (D) (i.e., 100 or 350 IU) estrus induction protocol (EIP) was evaluated. Goats (Alpine-Saanen-Nubian × Criollo; n = 70; 25° North) managed under stall-fed conditions were all ultrasound evaluated to confirm anestrous status, while the social rank was determined 30 d prior to the EIP. The response variables included estrus induction (EI, %), duration of estrus (DUR, h), ovulation rate (OR, n), live weight (LW, kg), thoracic perimeter (TP, cm), thoracic diameter (TD, cm), body length (BL, cm), height at withers (HW, cm), beard length (BEA, cm), compactness index (COM, cm), and anamorphosis index (ANA, cm), as affected by R, D, and the R × D interaction were evaluated, while the correlation coefficients among reproductive and morphometric variables were quantified. An R × D interaction (p < 0.05) affected the response variables EI, DUR, and OR. While the largest (p < 0.05) EI% occurred in the HSR goats, irrespective of eCG (i.e., 100 or 350 IU), both the shortest estrus duration (DUR, h) and the lowest ovulation rate (OR, n) occurred in the LSR + D100 combination, with no differences among HSR and MSR either with D100 or D350. Regarding the LW and morphometric response variables, (i.e., LW, TP, TD, BL, HW, BEA, COM, and ANA) all of them favored either the HSR and MSR groups, with the lowest phenotypic values occurring in the LSR-goats. The EI% was observed to be positively correlated (p < 0.05) with DUR (0.71), LW (0.28), TP (0.31), TD (0.34), BL (0.33), HW (0.35), COM (0.23), and ANA (0.23). While DUR was correlated (p < 0.05) with TP (0.26) and ANA (0.24), OR demonstrated no-correlation (p > 0.05) with any response variable, either reproductive or morphometric. As expected, LW had high correlation coefficients (p < 0.01) with TP (0.86), TD (0.88), BL (0.82), HW (0.75), BEA (0.51), COM (0.97), and ANA (0.75). In general, the morphometric variables as a whole demonstrated important correlation coefficients among them (p < 0.01), ranging from 0.38 up to 0.84. To estimate the importance of the morphometric differences between social rank upon estrus induction, a principal component (PC) analysis was performed based on the correlation matrix derived from the corporal measurements. The PC1 and PC2 explained 70.3% and 17.6% of the morphometric variation, respectively. The PC1 was a measure of the goat size (i.e., small, medium, large) and its association with estrus occurrence was evaluated using a logistic regression model; the bigger the goat, the increased probability of being in estrus, by up to five times compared to small goats. Our results confirm that the higher social ranked, larger goats amalgamated some fundamental factors to be successful: aggressiveness, primacy to food access, augmented live weight, and corporal size; all of these were able to modulate out-of-season reproductive success in crossbred dairy goats subjected to an estrus induction protocol and managed under stall-fed conditions in Northern Mexico.
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Humanos , Feminino , Infecções por Pseudomonas , Ectima/diagnóstico , Neutropenia , Pseudomonas aeruginosaRESUMO
Hepatic artery pseudoaneurysm is a rare condition; they are multifactorial, most of them locating in the extrahepatic vasculature and the mortality associated to its rupture may reach up to 70%. We report a 77 years old female who was admitted due to headache and uncontrolled hypertension and that on her second hospital day developed sudden hemodynamic instability, abdominal pain, fatigue, skin-mucosa pallor, and anemia. Abdominal CT scan with contrast showed a left hepatic artery pseudoaneurysm associated with extensive hemoperitoneum. Patient required emergent hemodynamic stabilization and finally was treated successfully with a superselective endovascular coil embolization. Our patient represents an atypical case of a spontaneous rupture of an idiopathic hepatic artery pseudoaneurysm. Hence, the importance of having a high index of clinical suspicion. Endovascular coil embolization has become the first-line treatment.
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Most living organisms possess an internal timekeeping mechanism known as the circadian clock, which enhances fitness by synchronizing the internal timing of biological processes with diurnal and seasonal environmental changes. In plants, the pace of these biological rhythms relies on oscillations in the expression level of hundreds of genes tightly controlled by a group of core clock regulators and co-regulators that engage in transcriptional and translational feedback loops. In the last decade, the role of several core clock genes in the control of defense responses has been addressed, and a growing amount of evidence demonstrates that circadian regulation is relevant for plant immunity. A reciprocal connection between these pathways was also established following the observation that in Arabidopsis thaliana, as well as in crop species like tomato, plant-pathogen interactions trigger a reconfiguration of the circadian transcriptional network. In this review, we summarize the current knowledge regarding the interaction between the circadian clock and biotic stress responses at the transcriptional level, and discuss the relevance of this crosstalk in the plant-pathogen evolutionary arms race. A better understanding of these processes could aid in the development of genetic tools that improve traditional breeding practices, enhancing tolerance to plant diseases that threaten crop yield and food security all around the world.
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Relógios Circadianos/genética , Interações Hospedeiro-Patógeno/genética , Plantas/genética , Transcrição Gênica/genética , Plantas/metabolismo , Plantas/microbiologiaRESUMO
We evaluated the possible role of the social rank [R] (i.e., low-LSR, middle-MSR, or high-HSR) in anestrus goats exposed to a P4 + eCG [D] (i.e., 100 or 350 IU) estrus induction protocol (EIP). Adult, multiparous (two to three lactations), multiracial, dairy-type goats (Alpine-Saanen-Nubian x Criollo goats (n = 70; 25°51' North) managed under stall-fed conditions were all ultrasound evaluated to confirm anestrus status while the R was determined 30 d prior to the EIP. The variables of estrus induction (EI, %), estrus latency (LAT, h), estrus duration (DUR, h), ovulation (OVU, %), ovulation rate (OR, n), corpus luteum size (CLS, cm), pregnancy (PREG, %), kidding (KIDD, %), and litter size (LS, n) as affected by R, D, and the R × D interaction, were evaluated. While OVU and CLS favored (p < 0.05) HSR (96% and + 1.04 ± 0.07 cm), an increased (p < 0.05) LS occurred in D350 vs. D100 (2.06 ± 0.2 vs. 1.36 ± 0.2); neither R nor D affected (p > 0.05; 38.5%) KIDD. However, EI, LAT, DUR, OR, and PREG were affected by the R × D interaction. The HSR group had the largest (p < 0.05) EI % and DUR h, irrespective of D. The shortest (p < 0.05) LAT occurred in D350, irrespective of R. While the largest (p < 0.05) OR occurred in HSR and MSR within D350, the HSR + D350 group had the largest PREG (p < 0.05). These research outcomes are central to defining out-of-season reproductive strategies designed to attenuate seasonal reproduction in goats.
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Although the incidence is uncertain, some case reports suggest that COVID 19 infection is associated with an increased risk of venous thromboembolism. We suggest starting prophylactic anticoagulant therapy for all patients hospitalized with a symptomatic infection with COVID-19, unless contraindicated, with enoxaparin 40 mg SC daily if creatinine clearance is greater than 30 ml/min.
Si bien la incidencia es incierta, algunos reportes de caso sugieren que la infección por COVID 19 se asocia con un aumento del riesgo de tromboembolismo venoso. Sugerimos iniciar tromboprofilaxis a todos los pacientes hospitalizados por síntomas asociados con una infección por COVID-19, a menos que esté contraindicado, con enoxaparina 40 mg SC diariamente si el clearance de creatinina es mayor a 30 ml/min.
Assuntos
Anticoagulantes/administração & dosagem , Coronavirus , Pacientes Internados , Tromboembolia/prevenção & controle , Tromboembolia Venosa/prevenção & controle , Anticoagulantes/uso terapêutico , Argentina , Betacoronavirus , COVID-19 , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/terapia , Humanos , Pandemias , Pneumonia Viral/epidemiologia , Pneumonia Viral/terapia , SARS-CoV-2Assuntos
Ectima , Neutropenia , Infecções por Pseudomonas , Ectima/diagnóstico , Feminino , Humanos , Pseudomonas aeruginosaRESUMO
Si bien la incidencia es incierta, algunos reportes de caso sugieren que la infección por COVID 19 se asocia con un aumento del riesgo de tromboembolismo venoso. Sugerimos iniciar tromboprofilaxis a todos los pacientes hospitalizados por síntomas asociados con una infección por COVID-19, a menos que esté contraindicado, con enoxaparina 40 mg SC diariamente si el clearance de creatinina es mayor a 30 ml/min.
Although the incidence is uncertain, some case reports suggest that COVID 19 infection is associated with an increased risk of venous thromboembolism. We suggest starting prophylactic anticoagulant therapy for all patients hospitalized with a symptomatic infection with COVID-19, unless contraindicated, with enoxaparin 40 mg SC daily if creatinine clearance is greater than 30 ml/min.
Assuntos
Humanos , Tromboembolia/prevenção & controle , Coronavirus , Tromboembolia Venosa/prevenção & controle , Pacientes Internados , Anticoagulantes/administração & dosagem , Argentina , Pneumonia Viral/terapia , Pneumonia Viral/epidemiologia , Infecções por Coronavirus/terapia , Infecções por Coronavirus/epidemiologia , Pandemias , Betacoronavirus , SARS-CoV-2 , COVID-19 , Anticoagulantes/uso terapêuticoRESUMO
The possible out-of-season effect of beta-carotene supplementation on ovulation rate (OR), antral follicles (AFN), and total ovarian activity (TOA = OR + AFN) as related to the LH release pattern in yearling anestrous goats was evaluated. In late April, Alpine-Saanen-Nubian x Criollo goats (n = 22, 26 N) were randomly allotted to: (1) Beta-carotene (BETA; n = 10, orally supplemented with 50 mg/goat/d; 36.4 ± 1.07 kg live weight (LW), 3.5 ± 0.20 units, body condition score (BCS) or (2) Non-supplemented (CONT; n = 12, 35.2 ± 1.07 kg LW, 3.4 ± 0.2 units BCS). Upon estrus synchronization, an intensive blood sampling (6 h × 15 min) was accomplished in May for LH quantifications; response variables included (pulsatility-PULSE, time to first pulse-TTFP, amplitude-AMPL, nadir-NAD and area under the curve-AUC). Thereafter, an ultrasonography scanning was completed to assess OR and AFN. The Munro algorithm was used to quantify LH pulsatility; if significant effects of time, treatment or interaction were identified, data were compared across time. Neither LW nor BCS (p > 0.05) or even the LH (p > 0.05); PULSE (4.1 ± 0.9 pulses/6 h), NAD (0.47 ± 0.13 ng) and AUC (51.7 ± 18.6 units) differed between treatments. Nonetheless, OR (1.57 vs. 0.87 ± 0.18 units) and TOA (3.44 vs. 1.87 ± 0.45 units) escorted by a reduced TTFP (33 vs. 126 ± 31.9 min) and an increased AMPL (0.55 vs. 0.24 ± 0.9 ng), favored to the BETA supplemented group (p < 0.05), possibly through a GnRH-LH enhanced pathway and(or) a direct effect at ovarian level. Results are relevant to speed-up the out-of-season reproductive outcomes in goats while may embrace translational applications.