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OBJECTIVE: Although prior research has shown that an array of distinct experiences related to crisis migration are associated with mental health, there is a pressing need for a theory-driven, multidimensional measure to assess the broad spectrum of crisis migration experiences. As such, the present study focused on developing and validating the Crisis Migration Experience Scale (CMES) with a sample of Venezuelan migrants in Colombia. METHOD: Participants were adolescent (ages 12-17; n = 430) and adult migrants from Venezuela (ages 18+; n = 569). Randomly splitting the adolescent and adult samples in half, exploratory factor analysis and confirmatory factor analyses were conducted with 26 original items. After identifying a satisfactory factor structure to generate a 16-item CMES (CMES-16), we examined the associations of the CMES-16 with mental health outcomes. RESULTS: We provide evidence for reliability, factorial validity, and concurrent validity of scores generated by the CMES-16 in a sample of Venezuelan crisis migrants in Colombia. Whereas our a priori conceptualization included seven domains, the exploratory and confirmatory factor analyses indicated that four are especially salient: material hardship, desperation, danger, and unplanned departure. CONCLUSIONS: Crisis migration is an increasingly important construct frequently referenced in the literature on migrant health and by international humanitarian organizations. The number of crisis migrant groups worldwide is increasing, with Ukrainians and Afghans recently added to the list of such groups, along with Venezuelans, Syrians, South Sudanese, Iraqis, and Central Americans. Developing and validating the CMES-16 with Venezuelan crisis migrants opens up important avenues of research, including work that incorporates other crisis migrant populations. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

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Favipiravir is an important selective antiviral that emerged as an alternative against COVID-19 during the pandemic. Its synthesis has gained great interest and the conventional strategies proceed through multiple-step protocols (6-7 reaction steps), which involve, in addition, several drawbacks with global yields, lower than 34%. Herein, a simple, economical, eco-friendly and scalable (1 g) one-step protocol for the synthesis of favipiravir from the direct fluorination of the available 3-hydroxy-2-pyrazinecarboxamide with Selectfluor® is reported. The reaction proceeds easily in BF4-BMIM through a simple operational work-up, affording the favipiravir with a yield of 50% without the need of a special catalyst/additive. The key point of the present strategy was the use of the ionic liquid of BF4-BMIM, which helps to minimize the several chemical limitations derived from 3-hydroxy-2-pyrazinecarboxamide as a substrate for the direct Selectfluor-mediated fluorination. All these chemical reactivity aspects are also discussed in detail.

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BACKGROUND: Multiple Myeloma (MM) is a malignant hematologic disorder and the second most common blood cancer. Interleukin-6 (IL-6) has been identified as a crucial factor for the proliferation and survival of MM cells and the overexpression of IL-6 receptor is being studied as a molecular target for therapeutic and diagnostic use in myelomas and other comorbidities. Tocilizumab is a humanized monoclonal antibody that binds IL-6R. OBJECTIVE: We aim to label and evaluate Fab(Tocilizumab) with 99mTechnetium or Cy7 as potential MM imaging agents. METHODS: IL-6R distribution was analyzed by Laser Confocal Microscopy (LCM) in MM cell lines. Fab(Tocilizumab) was produced by the digestion of Tocilizumab with papain for 24h at 37°C, derivatized with NHS-HYNIC-Tfa and radiolabeled with 99mTc. Radiochemical stability and in vitro cell assays were evaluated. Biodistribution and SPECT/CT were performed. Also, Fab(Tocilizumab) was labeled with Cy7 for in vivo fluorescence imaging up to 72h. RESULTS: LCM analysis demonstrates IL-6R distribution on MM cell lines. Incubation with papain resulted in complete digestion of Tocilizumab and exhibited a good purity and homogeneity. Radiolabeling with 99mTc via NHS-HYNIC-Tfa was found to be fast, easy, reproducible and stable, revealing high radiochemical purity and without interfering with IL-6R recognition. Biodistribution and SPECT/CT studies showed a quick blood clearance and significant kidney and MM engrafted tumor uptake. Cy7-Fab(Tocilizumab) fluorescent imaging allowed MM1S tumor identification up to 72h p.i. CONCLUSION: These new molecular imaging agents could potentially be used in the clinical setting for staging and follow-up of MM through radioactive whole-body IL-6R expression visualization in vivo. The fluorescent version could be used for tissue sample evaluation and to guide surgical excision, if necessary.

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The aim was to study number, volume, apoptosis of corpora lutea (CL), and serum P4 concentrations in early, middle, and late diestrus of dogs. Thirty-six bitches were ovari-hysterectomized (OVX): Early Diestrus (Group [G]1; OVX 20 days after end of estrus [DEH]); Mid-diestrus (GII; OVX between 21 and 40 days after DEH), and Late-diestrus (GIII; OVX between 41 and 60 days after DEH). Before OVX a blood sample was collected to quantify P4. After OVX, the number of CL (NCL) was recorded, CL measured using both ultrasonography (US) and caliper (CAL), and the volume (mm3) was calculated. Based on abundances of caspase-3, apoptotic luteal cells were detected. Bitches in early-diestrus had greater P4 concentrations than bitches in mid- and late-diestrus (23.52 ±â€¯3.78 and 10.86 ±â€¯3.88 ng/mL; P < 0.05). The NCL, cumulative USCLV, and CALCLV were similar among diestrus stages (P > 0.30). Bitches with CL (≥5) had twice the serum P4 concentrations as bitches with CL1-2 and CL3-4 (22.71 ±â€¯3.70 and 10.97 ±â€¯4.06 ng/mL; P < 0.05). There were correlations between P4 concentrations with USCLV, CALCLV, and NCL (r = 0.64, r = 0.67, r = 0.44; P < 0.0001). When serum P4 concentrations were adjusted for stages of diestrus, however, there were only correlations during early diestrus. The percentage of apoptotic cells was greater in GIII compared with GI and GII (13.75 ±â€¯2.26 % compared with 4.5 ±â€¯0.68 % and 4.6 ±â€¯1.5 %, respectively; P < 0.05). As days of diestrus increased, number of apoptotic cells increased, and serum P4 concentrations decreased.

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Rust diseases continuously threaten global wheat production: stem rust, leaf rust, and yellow rust caused by Puccinia graminis f. sp. tritici, Puccinia triticina, and Puccinia striiformis f. sp. tritici, respectively. Recent studies indicated that the average losses from all these three rusts reached up to 15.04 million tons per year, which is equivalent to an annual average loss of around US $2.9 billion per year. The major focus of Mexican and worldwide breeding programs is the release of rust resistant cultivars, as this is considered the best option for controlling rust diseases. In Mexico, the emphasis has been placed on genes that confer partial resistance in the adult plant stage and against a broad spectrum of rust races since the 1970s. In this study, a set of the first-generation tall varieties developed and released in the 1940s and 1950s, the first semi-dwarfs, and other releases in Mexico, all of which showed different levels of rust resistance have been phenotyped for the three rust diseases and genotyped. Results of the molecular marker detection indicated that Lr34, Lr46, Lr67, and Lr68 alone or in different gene combinations were present among the wheat cultivars. Flag leaf tip necrosis was present in all cultivars and most were positive for brown necrosis or Pseudo Black Chaff associated with the Sr2 stem rust resistance complex. The phenotypic responses to the different rust infections indicate the presence of additional slow rusting and race-specific resistance genes. The study reveals the association of the slow rusting genes with durable resistance to the three rusts including Ug99 in cultivars bred before the green revolution such as Frontera, Supremo 211, Chapingo 48, Yaqui 50, Kentana 52, Bajio 52, Bajio 53, Yaqui 53, Chapingo 53, Yaktana Tardio 54, and Mayo 54 and their descendants after intercrossing and recombination. These slow rusting genes are the backbone of the resistance in the current Mexican germplasm.

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OBJECTIVES: The current study aimed to evaluate the relationship between specific seminal plasma components - cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations - and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. METHODS: Thirteen toms, 2-5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at -20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats (P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. CONCLUSIONS AND RELEVANCE: Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

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The physiopathology of rheumatoid arthritis (RA) is mediated by proinflammatory cytokines, some of which are regulated by the JAK/STAT pathway. Tofacitinib is a JAK inhibitor, but its role in the regulation of microRNAs (miRNAs) is unknown. There is also no information regarding the role of miRNAs in the clinical relapse/remission of RA. The present project aims to identify a signature profile of miRNA expression in a subgroup of RA patients who had to discontinue tofacitinib treatment (because of the ending of a 5-year open-label clinical trial) and to describe the expression of miRNAs during RA remission or flare-up. The relative expression of 61 miRNAs was determined in serum samples with the Firefly™ BioWorks assay. Statistical analysis was performed by means of Student's t-test and heatmap analysis was performed with Firefly™ Analysis Workbench software and in the software GraphPad® Prism v5.0. Target prediction and Gene Ontology analysis were carried out using bioinformatic tools. We found a distinctive signature of miRNA expression associated with relapse, featuring upregulated expression of hsa­miR­432­5p (p < 0.05). We also found upregulation of hsa­miR­194­5p (p < 0.05) in samples of patients with RA flare-up. Gene Ontology analysis of the target genes for hsa­miR­432­5p was performed to identify relevant pathways associated with relapse; the implications of these pathways in the physiopathology of RA are discussed. Tofacitinib treatment does not have a direct effect on the expression of measured miRNAs. The changes in hsa­miR­432­5p and hsa­miR­194­5p are associated with the regulation of proinflammatory pathways and RA flare-up.

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RESUMEN Objetivo Estudiar el efecto de mantener un mismo diámetro de apertura en los dedos sobre la fuerza actuante de éstos en un agarre de fuerza. Métodos Se condujo un experimento con 30 participantes (15 hombres y 15 mujeres) quienes realizaron el gesto de aprehensión en un dispositivo diseñado para mantener constante la apertura para todos los dedos en 4 diámetros diferentes. Resultados Se muestra evidencia que en un rango de accionamiento entre 0-75% de la apertura funcional, los dedos índice, corazón y anular ejercen una fuerza significativamente igual si el diámetro de apertura se mantiene constante. Conclusiones Este hallazgo permitiría reducir la exposición de los operarios de herramientas de corte a factores de riesgo de síndrome del conducto carpiano (SCC). Se sugiere que en el diseño de herramientas, la distancia de apertura para todos los dedos debe mantenerse constante durante el gesto de aprehensión.(AU)

ABSTRACT Objective To study the effect of maintaining the same grip span between the thumb and exerting fingers, on the acting force of these in a power grip. Methods We conducted an experiment with 30 participants (15 men and 15 women) who made the gesture of apprehension in a device designed to maintain constant grip span to all fingers in 4 different diameters. Results In the range between 0-75 percent of functional grip span of the fingers, the index, middle and ring finger exert a force significantly equal when the aperture diameter is maintained constant. Conclusions These findings would reduce exposure to risk factors for carpal canal syndrome (CTS) in cutting activities, by controlling risk factors like strength. We suggest that on the design of tools, the opening distance for all the fingers must remain constant during the act of apprehension.(AU)

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Objetivo Determinar si un sistema basculante ubicado en el asiento de una silla favorece la realización de movimientos voluntarios de macro reposicionamiento de pelvis para mitigar la percepción de dolor en postura sedente prolongada. Método Ocho mujeres participaron en la prueba y permanecieron sentadas en sillas con y sin basculación durante 60 minutos, en dos jornadas diferentes conservando la hora de la prueba. La incomodidad fue evaluada con una escala visual analógica. Resultados Se encontró que las personas usaron el sistema basculante para realizar cambios posturales de macro reposicionamientos, sin embargo los realizados por éste son menores a los realizados por el mismo cuerpo, es decir sin ayuda del sistema. Además, la percepción de incomodidad fue igual en las dos sillas, posiblemente porque la cantidad total de macro reposicionamientos no varió. Conclusión Se sugiere que estudios futuros contemplen el diseño de sistemas basculantes que suministren macro reposicionamientos de forma controlada y no de forma voluntaria como se realizó en este trabajo. Así como estudiar si este tipo de sistemas automáticos permite mitigar la incomodidad debida al tiempo prolongado en postura sedente.(AU)

Objective To determine whether a tilting system located in the seat of a chair allows for performing voluntary macro-repositioning movements of the pelvis in order to reduce the perception of pain after a prolonged period in the sitting posture. Methods Eight women participated in the experiment. They were asked to sit on the chair with the tilting system and the chair without the tilting system for sixty minutes on two different days but carried out at the same time of day. Discomfort was assessed with the visual analogic scale. Results people used the tilting system to do postural changes of macro-repositioning. However, these movements were less than the movements done with the body, not using the system. Moreover, discomfort perception was the same for each chair. This might be due to the fact that total of macro-repositioning movements did not change. Conclusion Future research should evaluate the effect of tilting systems that provide macro-repositioning in a controlled form, not like in this study. Also, it is necessary to study if these types of automatic systems contribute to the reduction of the discomfort due to a prolonged period in the sitting posture.(AU)

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Objective To determine whether a tilting system located in the seat of a chair allows for performing voluntary macro-repositioning movements of the pelvis in order to reduce the perception of pain after a prolonged period in the sitting posture. Methods Eight women participated in the experiment. They were asked to sit on the chair with the tilting system and the chair without the tilting system for sixty minutes on two different days but carried out at the same time of day. Discomfort was assessed with the visual analogic scale. Results people used the tilting system to do postural changes of macro-repositioning. However, these movements were less than the movements done with the body, not using the system. Moreover, discomfort perception was the same for each chair. This might be due to the fact that total of macro-repositioning movements did not change. Conclusion Future research should evaluate the effect of tilting systems that provide macro-repositioning in a controlled form, not like in this study. Also, it is necessary to study if these types of automatic systems contribute to the reduction of the discomfort due to a prolonged period in the sitting posture.

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OBJECTIVE: To study the effect of maintaining the same grip span between the thumb and exerting fingers, on the acting force of these in a power grip. METHODS: We conducted an experiment with 30 participants (15 men and 15 women) who made the gesture of apprehension in a device designed to maintain constant grip span to all fingers in 4 different diameters. RESULTS: In the range between 0-75 percent of functional grip span of the fingers, the index, middle and ring finger exert a force significantly equal when the aperture diameter is maintained constant. CONCLUSIONS: These findings would reduce exposure to risk factors for carpal canal syndrome (CTS) in cutting activities, by controlling risk factors like strength. We suggest that on the design of tools, the opening distance for all the fingers must remain constant during the act of apprehension.

OBJETIVO: Estudiar el efecto de mantener un mismo diámetro de apertura en los dedos sobre la fuerza actuante de éstos en un agarre de fuerza. MÉTODOS: Se condujo un experimento con 30 participantes (15 hombres y 15 mujeres) quienes realizaron el gesto de aprehensión en un dispositivo diseñado para mantener constante la apertura para todos los dedos en 4 diámetros diferentes. RESULTADOS: Se muestra evidencia que en un rango de accionamiento entre 0-75% de la apertura funcional, los dedos índice, corazón y anular ejercen una fuerza significativamente igual si el diámetro de apertura se mantiene constante. CONCLUSIONES: Este hallazgo permitiría reducir la exposición de los operarios de herramientas de corte a factores de riesgo de síndrome del conducto carpiano (SCC). Se sugiere que en el diseño de herramientas, la distancia de apertura para todos los dedos debe mantenerse constante durante el gesto de aprehensión.

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Objetivo: Los desordenes músculo esqueléticos y especialmente el síndrome del conducto carpiano es uno de los problemas de salud pública más importantes de los últimos años. Es la mayor causa de morbilidad profesional en Colombia, y uno de los ámbitos laborales con mayor incidencia es el sector floricultor. El estudio se propuso establecer si la fuerza de aprehensión en el corte de flores se ve afectada por la altura del corte y la postura del brazo. Método: Participaron 12 operarias y se utilizó un arreglo factorial 2x2 con la altura de corte y postura en pronación o supinación del antebrazo como los factores que influyen en la fuerza de aprehensión. Resultados: El análisis estadístico muestra que no existe interacción entre los factores, pero si efectos importantes sobre la fuerza de aprehensión. La variación de la flexión del brazo tiene un mayor efecto en comparación con el cambio de la postura del antebrazo (pronación o supinación). Conclusiones: La postura ideal para realizar la tarea de corte es la pronación debido a que el esfuerzo necesario para realizar dicha tarea es menor. Se propone que las investigaciones futuras deben estudiar los efectos de las desviaciones de la muñeca en el sector floricultor.

Objective: Musculoskeletal disorders, particularly carpal tunnel syndrome, have been one of the most important public health problems during recent years. It is the leading cause of occupational morbidity in Colombia, the flower industry being one of the areas of work having the highest incidence. This study was thus aimed at ascertaining whether flower-cutters' strength of apprehension has been affected by cutting height and arm posture. Method: The study involved 12 operating personnel; a 22 factorial arrangement was used and the effect of cutting height and posture on forearm pronation or supination ans the factors influencing the strength of apprehension. Results: Statistical analysis showed no interaction between the factors; however, it did reveal a significant effect on the strength of apprehension. Varying how the arm bent had a greater effect than changing the position of the forearm (pronation or supination). Conclusions: Pronation was the ideal flower-cutting posture because it involved less effort to perform such task. Further research should examine the effects of wrist deviation angle in the flower industry.

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OBJECTIVE: Musculoskeletal disorders, particularly carpal tunnel syndrome, have been one of the most important public health problems during recent years. It is the leading cause of occupational morbidity in Colombia, the flower industry being one of the areas of work having the highest incidence. This study was thus aimed at ascertaining whether flower-cutters' strength of apprehension has been affected by cutting height and arm posture. METHOD: The study involved 12 operating personnel; a 22 factorial arrangement was used and the effect of cutting height and posture on forearm pronation or supination ans the factors influencing the strength of apprehension. RESULTS: Statistical analysis showed no interaction between the factors; however, it did reveal a significant effect on the strength of apprehension. Varying how the arm bent had a greater effect than changing the position of the forearm (pronation or supination). CONCLUSIONS: Pronation was the ideal flower-cutting posture because it involved less effort to perform such task. Further research should examine the effects of wrist deviation angle in the flower industry.

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Here we report quantitative data associating periodontal bone variables of young conventional rats with the growth process. The hemimandibles of male rats (IIM/Fm stock, 2 to 15 wk of age.) were excised and submitted to conventional morphologic, radiologic, and histologic evaluation. The length, area, or X-ray absorbance of various regions or structures was measured on digital images of radiographs by using an image-analysis program. The sum of periodontal bone areas undergoing resorption (interproximal + intraradicular) increased until 9 or 10 wk of age and decreased thereafter. Mineral accretion rates and mineral density asymptotes were not significantly different among molars. The mineral density of resorption areas in alveolar bone fitted sinusoidal kinetics, indicative of the 'instability' of the tissue due to its high metabolic activity. Mineral accretion rates and mineral density asymptotes were not significantly different among molars. The proportion of root length within alveolar bone exhibited a biphasic curve (minimum at 5 wk of age), due to differences in the growth rates of variables involved in its calculation (distance between the cementoenamel junction to the apex and height of the resorption areas). The distance between the cementoenamel junction and alveolar bone crest over time fitted a sigmoidal function with a point of inflection that did not differ significantly from that of body or mandible dry weight. In summary, the growth process appears to affect periodontal bone support and the distance between the cementoenamel junction and alveolar bone crest in male rats.

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