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1.
BMC Womens Health ; 24(1): 550, 2024 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-39367365

RESUMO

BACKGROUND: Mexico reports low follow-up completion rates among women with abnormal cervical cancer screenings. This study aimed to identify barriers and facilitators to follow-up adherence among women with human papillomavirus (HPV) infection and premalignant cervical lesions in Mexico. METHODS: A mixed-methods study was conducted from February to April 2019. Participants included women undergoing follow-up care for high-risk human papillomavirus (HR-HPV) and premalignant lesions, along with health personnel from the Women's Healthcare Center (CAPASAM) in Mexico. Quantitative data were obtained from the Women's Cancer Information System and through a questionnaire about factors affecting follow-up adherence. Additionally, the health personnel involved completed a compliance checklist regarding care regulations. Descriptive statistics were used for analysis. Qualitative data were collected via semi-structured interviews with both groups, followed by a content analysis based on identified categories. The Hazard Analysis and Critical Control Point System confirmed care process risks. Proposals to enhance the Early Detection Program for Prevention and Control of Cervical Cancer were collected from a CAPASAM health personnel nominal group. RESULTS: Identified barriers to follow-up included low income among CAPASAM users, family provider roles limiting time for appointments, long waits for testing and results delivery, distant facilities, insufficient service hour communication, inadequate health personnel training, and a lack of systematic counseling. Hesitation toward follow-up was also linked to shame, apprehension, uncertainty, test aversion, fear of positive results, and limited cervical cancer and screening knowledge. Patriarchal attitudes of partners and limited access to the now-discontinued PROSPERA government program further discouraged follow-up. Facilitators comprised respectful treatment by CAPASAM staff, no-cost services, health campaigns, and positive user attitudes. CONCLUSIONS: The study found more barriers than facilitators to follow-up adherence, highlighting the need for strategies to bolster the Early Detection Program. Future strategies must address the comprehensive array of factors and incorporate stakeholder perspectives.


Assuntos
Detecção Precoce de Câncer , Infecções por Papillomavirus , Lesões Pré-Cancerosas , Neoplasias do Colo do Útero , Humanos , Feminino , México , Adulto , Neoplasias do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/psicologia , Infecções por Papillomavirus/psicologia , Pessoa de Meia-Idade , Detecção Precoce de Câncer/métodos , Lesões Pré-Cancerosas/diagnóstico , Lesões Pré-Cancerosas/psicologia , Cooperação do Paciente/estatística & dados numéricos , Cooperação do Paciente/psicologia , Inquéritos e Questionários , Displasia do Colo do Útero/psicologia , Displasia do Colo do Útero/diagnóstico , Pesquisa Qualitativa , Seguimentos , Adulto Jovem
2.
Clin Respir J ; 18(7): e13813, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39013440

RESUMO

OBJECTIVE: The objective of this study was to associate the epidemiological and clinical characteristics of patients hospitalized for COVID-19 with the progression to critical illness and death in northwestern Mexico. METHODS: From March to October 2020, we collected the demographic and clinical characteristics of 464 hospitalized patients from northwestern Mexico. RESULTS: Sixty-four percent (295/464) of the patients became critically ill. Age, occupation, steroid and antibiotic use at previous hospitalization, and underlying diseases (hypertension, obesity, and chronic kidney disease) were associated with critical illness or death (p: < 0.05). No symptoms were associated with critical illness. However, the parameters such as the heart rate, respiratory rate, oxygen saturation, and diastolic pressure and the laboratory parameters such as the glucose, creatinine, white line cells, hemoglobin, D-dimer, and C-reactive protein, among others, were associated with critical illness (p: < 0.05). Finally, advanced age, previous hospital treatment, and the presence of one or more underlying diseases were associated with critical illness and death (p: < 0.02). CONCLUSIONS: Several epidemiological (e.g., age and occupation) and clinical factors (e.g., previous treatment, underlying diseases, and vital signs and laboratory parameters) were associated with critical illness and death in patients hospitalized with COVID-19. These data provide us with possible markers to avoid critical illness or death from COVID-19 in our region.


Assuntos
COVID-19 , Estado Terminal , Progressão da Doença , Hospitalização , SARS-CoV-2 , Humanos , COVID-19/epidemiologia , COVID-19/mortalidade , México/epidemiologia , Masculino , Feminino , Pessoa de Meia-Idade , Estudos Retrospectivos , Estado Terminal/mortalidade , Estado Terminal/epidemiologia , Hospitalização/estatística & dados numéricos , Idoso , Adulto , Fatores de Risco , Mortalidade Hospitalar/tendências , Pandemias
3.
Food Microbiol ; 123: 104567, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39038901

RESUMO

This study aimed to determine the prevalence of V. parahaemolyticus in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify V. parahaemolyticus from oysters; antiserum reaction to determine V. parahaemolyticus serotypes, and PCR assays were performed to identify pathogenic (tdh and/or trh) or pandemic (toxRS/new, and/or orf8) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for V. parahaemolyticus. Forty-seven percent of oyster samples were positive for V. parahaemolyticus. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.


Assuntos
Antibacterianos , Ostreidae , Frutos do Mar , Vibrio parahaemolyticus , Fatores de Virulência , Animais , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/efeitos dos fármacos , Vibrio parahaemolyticus/isolamento & purificação , México/epidemiologia , Ostreidae/microbiologia , Fatores de Virulência/genética , Antibacterianos/farmacologia , Frutos do Mar/microbiologia , Farmacorresistência Bacteriana , Sorogrupo , Virulência/genética , Testes de Sensibilidade Microbiana
4.
Arch. latinoam. nutr ; Arch. latinoam. nutr;74(2): 141-150, jun. 2024. ilus, tab, graf
Artigo em Espanhol | LILACS, LIVECS | ID: biblio-1561627

RESUMO

Introducción: La celiaquía es una patología multisistémica con base autoinmune que provoca una reacción a la proteína gluten encontrada en alimentos como o trigo, centeno, triticale, cebada, espelta y avena. Los síntomas más comunes de la celiaquía son la pérdida de peso, pérdida de apetito y fatiga general entre otros. A consecuencia de estos síntomas el rendimiento deportivo puede verse afectado. Objetivo: Para comprender como la celiaquía impacta en el rendimiento de los deportistas, el objetivo de este estudio es realizar una revisión sistemática relacionada con el rendimiento deportivo en personas celíacas. Materiales y métodos: Para ello, se utilizó la siguiente frase final de búsqueda: "Celiac" AND "Sport" AND "Performance", que fue introducida por igual en las bases de datos: Web of Science, Scopus y PubMed. Resultados: Los resultados muestran la existencia de escasos documentos relacionados con el objeto de estudio (n=11), dónde se muestra una variabilidad de tipos de diseño en los trabajos seleccionados. Por otro lado, se extrae que la única solución para mantener un adecuado rendimiento deportivo en una persona celiaca es llevar una dieta libre de gluten de por vida. Conclusiones: Los documentos seleccionados subrayan la importancia de que los deportistas con enfermedad celiaca mantengan una dieta sin gluten de manera constante a lo largo de su vida. Debido a la escasez de estudios relacionados con la temática, se recomienda aumentar el número de investigaciones relacionadas con el objeto de estudio, con la finalidad de mejorar los estilos y hábitos de vida de los deportistas celíacos(AU)


Introduction: Celiac disease is a multisystem autoimmune disorder that triggers a reaction to the protein gluten found in foods such as wheat, rye, triticale, barley, spelt, and oats. The most common symptoms of coeliac disease are weight loss, loss of appetite and general fatigue among others. As a result of these symptoms, athletic performance may be affected. Objetive: To understand how celiac disease impacts athletes' performance, the aim of this study is to conduct a systematic review related to sports performance in individuals with celiac disease. Materials and method: To do so, the following search phrase was used: "Celiac" AND "Sport" AND "Performance", which was entered equally in the following databases: Web of Science, Scopus and PubMed. Results: The results show the existence of few documents related to the object of study (n=11), where a variability of design types is shown in the selected works. On the other hand, the only solution to maintain adequate sports performance in a person with coeliac disease is to follow a gluten-free diet for life. Conclusions: The selected documents emphasize the importance of athletes with celiac disease maintaining a gluten-free diet consistently throughout their lives. Due to the scarcity of studies related to this topic, it is recommended to increase the number of research studies on this subject in order to improve the lifestyles and habits of athletes with celiac disease(AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Doença Celíaca/complicações , Desempenho Atlético , Dieta Livre de Glúten , Glutens , Sinais e Sintomas , Fadiga , Sistema Imunitário , Enteropatias , Estilo de Vida
5.
Entropy (Basel) ; 26(6)2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38920534

RESUMO

This paper extends the concept of metrics based on the Bayesian information criterion (BIC), to achieve strongly consistent estimation of partition Markov models (PMMs). We introduce a set of metrics drawn from the family of model selection criteria known as efficient determination criteria (EDC). This generalization extends the range of options available in BIC for penalizing the number of model parameters. We formally specify the relationship that determines how EDC works when selecting a model based on a threshold associated with the metric. Furthermore, we improve the penalty options within EDC, identifying the penalty ln(ln(n)) as a viable choice that maintains the strongly consistent estimation of a PMM. To demonstrate the utility of these new metrics, we apply them to the modeling of three DNA sequences of dengue virus type 3, endemic in Brazil in 2023.

6.
J Plant Physiol ; 297: 154259, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38705079

RESUMO

Management of the plant microbiome may help support food needs for the human population. Bacteria influence plants through enhancing nutrient uptake, metabolism, photosynthesis, biomass production and/or reinforcing immunity. However, information into how these microbes behave under different growth conditions is missing. In this work, we tested how carbon supplements modulate the interaction of Pseudomonas chlororaphis with Arabidopsis thaliana. P. chlororaphis streaks strongly repressed primary root growth, lateral root formation and ultimately, biomass production. Noteworthy, increasing sucrose availability into the media from 0 to 2.4% restored plant growth and promoted lateral root formation in bacterized seedlings. This effect could not be observed by supplementing sucrose to leaves only, indicating that the interaction was strongly modulated by bacterial access to sugar. Total phenazine content decreased in the bacteria grown in high (2.4%) sucrose medium, and conversely, the expression of phzH and pslA genes were diminished by sugar supply. Pyocyanin antagonized the promoting effects of sucrose in lateral root formation and biomass production in inoculated seedlings, indicating that this virulence factor accounts for growth repression during the plant-bacterial interaction. Defence reporter transgenes PR-1::GUS and LOX2::GUS were induced in leaves, while the expression of the auxin-inducible, synthetic reporter gene DR5::GUS was enhanced in the roots of bacterized seedlings at low and high sucrose treatments, which suggests that growth/defence trade-offs in plants are critically modulated by P. chlororaphis. Collectively, our data suggest that bacterial carbon nutrition controls the outcome of the relation with plants.


Assuntos
Arabidopsis , Ácidos Indolacéticos , Fenazinas , Raízes de Plantas , Pseudomonas chlororaphis , Sacarose , Sacarose/metabolismo , Arabidopsis/microbiologia , Arabidopsis/metabolismo , Arabidopsis/genética , Raízes de Plantas/microbiologia , Raízes de Plantas/metabolismo , Pseudomonas chlororaphis/metabolismo , Fenazinas/metabolismo , Ácidos Indolacéticos/metabolismo
7.
Gels ; 10(5)2024 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-38786231

RESUMO

Food gels are viscoelastic substances used in various gelled products manufactured around the world. Polysaccharides are the most common food gelling agents. The aim of this work was the production and characterization of a gel produced in a blue corn flour fermentation process, where different proportions were used of blue corn (Zea mays L.) flour and Czapek Dox culture medium (90 mL of culture medium with 10 g of blue corn flour, 80 mL of culture medium with 20 g of blue corn flour, and 70 mL of culture medium with 30 g of blue corn flour) and were fermented for three different durations (20, 25, and 30 days) with the Colletotrichum gloeosporioides fungus. A characterization of the gel was carried out studying the rheological properties, proximal analysis, toxicological analysis, microscopic structure, and molecular characterization, in addition to a solubility test with three different organic solvents (ethanol, hexane, and ethyl acetate, in addition to water). The results obtained showed in the rheological analysis that the gel could have resistance to high temperatures and a reversible behavior. The gel is soluble in polar solvents (ethanol and water). The main chemical components of the gel are carbohydrates, especially polysaccharides, and it was confirmed by FT-IR spectroscopy that the gel may be composed of pectin.

8.
Sci Rep ; 14(1): 8446, 2024 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-38600186

RESUMO

Acting as a goalkeeper in a video-game, a participant is asked to predict the successive choices of the penalty taker. The sequence of choices of the penalty taker is generated by a stochastic chain with memory of variable length. It has been conjectured that the probability distribution of the response times is a function of the specific sequence of past choices governing the algorithm used by the penalty taker to make his choice at each step. We found empirical evidence that besides this dependence, the distribution of the response times depends also on the success or failure of the previous prediction made by the participant. Moreover, we found statistical evidence that this dependence propagates up to two steps forward after the prediction failure.

9.
Foods ; 13(7)2024 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-38611287

RESUMO

Fungal infestations, particularly from Rhizopus stolonifer, pose significant post-harvest challenges for strawberries, compromising their shelf life and quality. Traditional preservation methods, including refrigeration, offer limited protection against such pathogens. This study introduces an innovative approach, utilizing edible films infused with Bacillus subtilis strains GOS 01 B-67748 and HFC 103, known for their antifungal properties. We demonstrate that these bioactive films not only inhibit fungal growth effectively but also enhance the preservation of strawberries at varying temperatures. The inclusion of Bacillus subtilis in edible films represents a significant advancement in extending the viability of strawberries, surpassing the efficacy of conventional methods. Our findings suggest a promising avenue for natural, safe food preservation techniques, aligning with current consumer preferences for additive-free products. This research contributes to the broader understanding of microbial-based food preservation strategies, offering potential applications across a range of perishable commodities.

10.
ACS Omega ; 9(8): 9526-9535, 2024 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-38434895

RESUMO

This study addresses the fabrication of extruded films using poly(lactic acid) (PLA) and chitosan, with and without maleic anhydride as a compatibilizing agent, for potential applications in disposable food packaging. These films underwent controlled conditions of UV irradiation, water condensation, and temperature variations in an accelerated weathering chamber. The investigation analyzed the effect of different exposure periods on the structural, morphological, mechanical, and thermal properties of the films. It was observed that PLA films exhibited a lower susceptibility to degradation compared to those containing chitosan. Specifically, the pure PLA film showed an increase in elastic modulus and strength during the initial 144 h of exposure, associated with cross-linking induced by UV radiation. On the other hand, film Q2 composed of PLA, chitosan, and maleic anhydride and Q1 without maleic anhydride experienced a tensile strength loss of over 50% after 244 h of exposure. The Q2 film exhibited greater homogeneity, leading to increased resistance to degradation compared to that of Q1. As the degradation time increased, both the Q1 and Q2 films demonstrated a decline in thermal stability. These films also exhibited alterations in crystallinity attributed to the chemo-crystallization process, along with fluctuations in the glass transition temperature and crystallization, particularly at 288 h.

11.
Am J Case Rep ; 25: e942974, 2024 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-38526305

RESUMO

BACKGROUND The VACTEREL association is an acronym that includes vertebral malformations (V), anal atresia (A), cardiac defects (C), tracheoesophageal fistula (TE), renal defects (R), and limb malformations (L). The aortic arch is the section between the ascending aorta and the descending aorta, where some variants have been described, such as the right aortic arch and bovine aortic arch, among others. A rare presentation in the Natsis classification is the "type X" where a bovine aortic arch and anomalous origin of the left vertebral artery are present. Several structural cardiac malformations have been described in the VACTEREL association. Still, there is no bovine arch or an anomalous left vertebral artery. CASE REPORT Our patient was a 3-year-old boy with a diagnosis of VACTEREL association (type III esophageal atresia, congenital hip dislocation, scoliosis, bilateral clubfoot, and grade IV biliary ureteral reflux). Echocardiographic findings showed changes in the aortic arch, and angiotomography and magnetic resonance angiography showed a bovine aortic arch and an anomalous left vertebral artery. At the time of diagnosis, there were no clinical manifestations or complications due to the anomalous origin of the left vertebral artery. CONCLUSIONS This is the first description of a bovine type X arch according to the Natsis classification in a VACTEREL association. In general, knowledge of the anatomical variants of the aortic arch and the origin and course of the vertebral arteries is of great clinical and interventional importance, mainly because of the risk of cerebral ischemia.


Assuntos
Canal Anal/anormalidades , Aorta Torácica , Esôfago/anormalidades , Cardiopatias Congênitas , Rim/anormalidades , Deformidades Congênitas dos Membros , Coluna Vertebral/anormalidades , Traqueia/anormalidades , Masculino , Humanos , Pré-Escolar , Aorta Torácica/diagnóstico por imagem , Artéria Vertebral , Aorta , Deformidades Congênitas dos Membros/diagnóstico por imagem
12.
BMJ Case Rep ; 17(3)2024 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-38514153

RESUMO

Ewing sarcoma is an exceedingly rare form of cancer that affects the cervix. It falls within the spectrum of neoplastic diseases known as Ewing's family of tumours, typically observed in osseous tissues. A woman in her 40s, experiencing symptoms of leucorrhoea and transvaginal bleeding that commenced 3 months before her consultation, was referred to our gynaecological oncology clinic with a preliminary diagnosis of ovarian teratoma. A colposcopy procedure was conducted unveiling a complete loss of cervical anatomy with friable and malodorous tissue. Pelvic ultrasound identified a lesion of uncertain origin in the cervix, suggestive of malignancy. Histopathological assessment of cervical biopsy specimens confirmed the presence of a small, round, blue cell neoplasm consistent with Ewing sarcoma. She underwent chemotherapy and pelvic radiotherapy, achieving complete remission 9 months after diagnosis, without experiencing any systemic adverse effects or sequelae.


Assuntos
Sarcoma de Ewing , Feminino , Humanos , Sarcoma de Ewing/diagnóstico por imagem , Sarcoma de Ewing/terapia , Colo do Útero/patologia
13.
JBRA Assist Reprod ; 28(2): 295-298, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38530762

RESUMO

OBJECTIVE: Late follicular phase progesterone elevation is a complication that affects approximately 38% of IVF cycles. There is a lack of consensus on the appropriate cut-off levels for progesterone on hCG day. Although premature progesterone rise occurs in all kinds of ovarian responses, there is a knowledge gap regarding the ovarian response with the highest risk of this phenomenon. Our study aims to assess the relative risk of each kind of ovarian response for premature progesterone rise and evaluate the prevalence of premature progesterone rise in each ovarian response. METHODS: A retrospective, cross-sectional, comparative and analytic study was performed at the Reproductive Endocrinology Department in Centro Médico Nacional 20 de Noviembre in Mexico City. All conventional-antagonist cycles were grouped according to their ovarian response and were evaluated from 2015 to 2020. Pearson's Squared-chi, Cramer's V, cross-table and the relative risk were calculated. RESULTS: The prevalence of premature progesterone rise oscillated from 20.8 to 67.9% for low and high ovarian responders, respectively. After calculating the relative risk, high ovarian responders had a 1.38 higher risk for premature progesterone rise than other groups. CONCLUSIONS: High ovarian responders have the highest risk for premature progesterone rise compared to normal and low ovarian responders. High ovarian responders have a 67.9% prevalence of premature progesterone rise.


Assuntos
Fertilização in vitro , Indução da Ovulação , Progesterona , Humanos , Feminino , Progesterona/sangue , Estudos Retrospectivos , Estudos Transversais , Indução da Ovulação/métodos , Indução da Ovulação/estatística & dados numéricos , Adulto , Fertilização in vitro/métodos , Fertilização in vitro/estatística & dados numéricos , Gravidez , Fase Folicular , México/epidemiologia
14.
Plants (Basel) ; 13(1)2024 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-38202439

RESUMO

When colonizing new ranges, plant populations may benefit from the absence of the checks imposed by the enemies, herbivores, and pathogens that regulated their numbers in their original range. Therefore, rates of plant damage or infestation by natural enemies are expected to be lower in the new range. Exposing both non-native and native plant populations in the native range, where native herbivores are present, can be used to test whether resistance mechanisms have diverged between populations. Datura stramonium is native to the Americas but widely distributed in Spain, where populations show lower herbivore damage than populations in the native range. We established experiments in two localities in the native range (Mexico), exposing two native and two non-native D. stramonium populations to natural herbivores. Plant performance differed between the localities, as did the abundance of the main specialist herbivore, Lema daturaphila. In Teotihuacán, where L. daturaphila is common, native plants had significantly more adult beetles and herbivore damage than non-native plants. The degree of infestation by the specialist seed predator Trichobaris soror differed among populations and between sites, but the native Ticumán population always had the lowest level of infestation. The Ticumán population also had the highest concentration of the alkaloid scopolamine. Scopolamine was negatively related to the number of eggs deposited by L. daturaphila in Teotihuacán. There was among-family variation in herbivore damage (resistance), alkaloid content (scopolamine), and infestation by L. daturaphila and T. soror, indicating genetic variation and potential for further evolution. Although native and non-native D. stramonium populations have not yet diverged in plant resistance/constitutive defense, the differences between ranges (and the two experimental sites) in the type and abundance of herbivores suggest that further research is needed on the role of resource availability and adaptive plasticity, specialized metabolites (induced, constitutive), and the relationship between genealogical origin and plant defense in both ranges.

15.
JBRA Assist Reprod ; 28(2): 247-253, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38289204

RESUMO

OBJECTIVE: Does progesterone levels on hCG day influence maturation rates and number of mature oocytes during ovarian stimulation for IVF/ICSI cycles?. METHODS: A retrospective, observational, analytic, cross-sectional and cohort study was performed at the Reproductive Endocrinology Department of the Centro Médico Nacional 20 de Noviembre in Mexico City between 2015 to 2020. All female patients underwent an ovarian stimulation cycle for IVF/ICSI, either with a mild or conventional stimulation protocol. Patients were classified according to their progesterone levels, Group 1 <1.5ng/ml and Group 2 >1.5mg/ml. A Spearman Rho test, a simple linear regression model, a Principal Component Analysis and a Student's T-test, were performed. RESULTS: A total of 600 patients were included. The overall results showed that there is a positive correlation between the number of retrieved, mature oocytes and progesterone levels on HCG day. After the Principal Component Analysis we observed that poor ovarian responders had the lowest maturation rate and number of mature oocytes. While the Student's t test showed that progesterone levels beyond 1.5ng/ml are associated to a higher number of mature oocytes but not a better maturation rate. CONCLUSIONS: Higher serum progesterone levels are associated with increased retrieved and mature oocytes in high responders. At the same time, higher progesterone levels in lower responders are not associated with optimal ovarian response.


Assuntos
Gonadotropina Coriônica , Fertilização in vitro , Oócitos , Indução da Ovulação , Progesterona , Humanos , Feminino , Progesterona/sangue , Adulto , México , Estudos Retrospectivos , Indução da Ovulação/métodos , Gonadotropina Coriônica/sangue , Estudos Transversais , Fertilização in vitro/métodos , Gravidez , Estudos de Coortes
16.
Clin Rheumatol ; 43(1): 501-509, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37964076

RESUMO

OBJECTIVE: To address the relationship between systemic lupus erythematosus (SLE) disease activity and the functional parameters of the innate immunity. METHODS: We evaluated a cohort of 26 adult SLE patients and 10 sex and age-paired healthy donors. When the patients had a disease flare (baseline) and when they achieve clinical response (follow-up), we assessed the systemic lupus erythematosus disease activity index 2 K (SLEDAI 2 K) and the following parameters with flow cytometry and confocal microscopy: monocyte subsets, their expression of TLR2, phagocytic monocytes and neutrophils using the pHrodo Red E. coli BioParticles, the respiratory burst with 123-dihydrorhodamine in neutrophils, and the spontaneous and lipopolysaccharide (LPS)-induced production of neutrophil extracellular traps (NETs). We used the Wilcoxon test to compare the paired medians with interquartile range (IQR) and the Mann-Whitney U test for independent medians. To assess the effect of prednisone and SLEDAI 2 K on the mentioned parameters, we applied a generalized mixed linear model. RESULTS: Twenty-three patients (88.4%) were women. The SLEDAI 2 K was higher at baseline 8 (6-14) in comparison to that at follow-up (6 (4-8), P = 0.028). At baseline, SLE patients had a decreased percentage of intermediate monocytes, a higher expression of TLR2 in total monocytes, increased phagocytosis in monocytes and neutrophils, a decreased respiratory burst intensity, and an increased production of NETs. In the mix model, the SLEDAI 2 K was the main factor influencing these functional innate immune parameters. CONCLUSION: Disease activity regulates the innate immune function in SLE which may contribute to the clinical features and infection predisposition. Key points • This is the first cohort study addressing the effect of disease activity and prednisone use on the innate immune function of lupus patients. • Our results show that the disease activity is a key regulator of the respiratory burst, phagocytosis, and the production of neutrophil extracellular traps. • Also, we observed a differential proportion of monocyte subsets according to SLE disease activity. • We consider that our manuscript contributes to the evidence addressing the intrinsic immune abnormalities of patients with SLE regardless of the use of immunosuppressants and set the bases for new research work considering the disease activity as an element to decide the prescription and duration of antibiotic prophylaxis in SLE patients, which is of interest to all rheumatologists.


Assuntos
Lúpus Eritematoso Sistêmico , Receptor 2 Toll-Like , Adulto , Humanos , Feminino , Masculino , Prednisona/uso terapêutico , Estudos de Coortes , Escherichia coli , Lúpus Eritematoso Sistêmico/tratamento farmacológico , Imunidade
17.
Neotrop Entomol ; 53(2): 364-371, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38133732

RESUMO

Hymenoepimecis is a genus of Darwin wasps in the Polysphincta group of genera (Hymenoptera: Ichneumonidae: Pimplinae) known as ectoparasitoids of a broad spectrum of spiders. The parasitoid induces production of a web known as cocoon web, which provides shelter and support for the wasp pupa. In this study, we describe for the first time the interaction between Hymenoepimecis castilloi Pádua & Sääksjärvi (Hymenoptera: Ichneumonidae) and its host spider Leucauge mariana (Taczanowski) (Araneae: Tetragnathidae) in the Colombian Andes, provide new records of wasp genus distribution, and described the behavioral modifications induced in the spider. Web modifications occurred in the webs of both solitary and aggregated individuals. Adhesive spirals were lacking, and webs were connected to vegetation by multiple threads in all cocoon webs, which was not seen attached to webs of non-parasitized spiders. All parasitoid cocoons were observed hanging on a vertical line in the hub of the cocoon web. As previously described for other species, we believe that this modified web design results in increased web strength and favors parasitoid development during the pupal stage.


Assuntos
Parasitos , Aranhas , Vespas , Humanos , Animais , Colômbia , Pupa
18.
J Fungi (Basel) ; 9(12)2023 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-38132728

RESUMO

This study analyzed the role of blood serum in enhancing the mitochondrial metabolism and virulence of Mucorales through rhizoferrin secretion. We observed that the spores of clinically relevant Mucorales produced in the presence of serum exhibited higher virulence in a heterologous infection model of Galleria mellonella. Cell-free supernatants of the culture broth obtained from spores produced in serum showed increased toxicity against Caenorhabditis elegans, which was linked with the enhanced secretion of rhizoferrin. Spores from Mucoralean species produced or germinated in serum showed increased respiration rates and reactive oxygen species levels. The addition of non-lethal concentrations of potassium cyanide and N-acetylcysteine during the aerobic or anaerobic growth of Mucorales decreased the toxicity of the cell-free supernatants of the culture broth, suggesting that mitochondrial metabolism is important for serum-induced virulence. In support of this hypothesis, a mutant strain of Mucor lusitanicus that lacks fermentation and solely relies on oxidative metabolism exhibited virulence levels comparable to those of the wild-type strain under serum-induced conditions. Contrary to the lower virulence observed, even in the serum, the ADP-ribosylation factor-like 2 deletion strain exhibited decreased mitochondrial activity. Moreover, spores produced in the serum of M. lusitanicus and Rhizopus arrhizus that grew in the presence of a mitophagy inducer showed low virulence. These results suggest that serum-induced mitochondrial activity increases rhizoferrin levels, making Mucorales more virulent.

19.
Actual. psicol. (Impr.) ; 37(135): 29-43, jul.-dic. 2023. tab, graf
Artigo em Espanhol | LILACS, SaludCR | ID: biblio-1556777

RESUMO

Resumen. Objetivo. Analizar el efecto de las expectativas y metas de progreso académicas en las dimensiones de la calidad de vida de estudiantes universitarios mexicanos. Método. El tipo de investigación es cuantitativa con un diseño transversal, abierto, observacional con alcances descriptivos y predictivos. Las técnicas estadísticas utilizadas fueron estadística descriptiva, coeficiente de correlación y análisis de senderos con el método GLS. El muestreo fue no probabilístico por conveniencia, con un total de 465 participantes. Resultados. Se demostró que, para el modelo explicativo resultante, la variable metas de progreso académicas es un predictor directo de la dimensión función física. Asimismo, las expectativas académicas de resultado son un predictor de las dimensiones rol emocional y la función física. Respecto a los componentes de la salud física y mental, este último presentó la puntuación más baja.


Abstract. Objective. To analyze the effect of expectations and goals of academic progress on the dimensions of the quality of life of Mexican university students. Method. The research was quantitative with a cross-sectional, open, observational design with descriptive and predictive scopes. The statistical techniques used were descriptive statistics, correlation coefficient and path analysis with the GLS method. Sampling was non-probabilistic for convenience, with a total of 465 participants. Results. For the resulting explanatory model the variable academic progress goals is a direct predictor of the physical function dimension. And academic expectations of outcome are a predictor of the emotional role and physical function dimensions.


Assuntos
Humanos , Masculino , Feminino , Adulto , Qualidade de Vida/psicologia , Desempenho Acadêmico/psicologia , Estudantes/psicologia , México
20.
BMJ Case Rep ; 16(11)2023 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-37945280

RESUMO

The presence of mature bone and bone marrow in the thyroid gland is an exceedingly rare occurrence. Extramedullary haematopoiesis (EMH) and heterotopic bone formation (HBF) should be suspected when cytology of thyroid nodules reveals evidence of megakaryocytes or bone marrow fat, respectively. The cause of these abnormalities has not been fully elucidated, but the role of bone morphogenic factors (BMPs) in their pathogenesis has been suggested. Both EMH and HBF can be seen in both benign and malignant primary thyroid conditions, and although they have not been definitively associated with significant pathology, it is recommended that extension studies be considered in the event of these findings to rule out concomitant haematological conditions.


Assuntos
Doenças Hematológicas , Hematopoese Extramedular , Ossificação Heterotópica , Nódulo da Glândula Tireoide , Humanos , Ossificação Heterotópica/diagnóstico por imagem , Nódulo da Glândula Tireoide/diagnóstico por imagem , Feminino , Idoso
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