RESUMO
Se examino una vacuna diseñada para inmunizar al hombre, preparada con extracto de fenol insoluble, para determinar si protegia a cobayos contra el desafio con la cepa virulenta B. abortus 2308. Se incluyeron en el experimento las vacunas vivas atenuadas B. abortus cepa 19 y B. melitensis Rev. 1, para comparar los resultados. Se vacunaron 93 animales en cada grupo, que fueron subdivididos en subgrupos de 31 y se los desafio con '10 POT. 4', '10 POT. 3' Y '10 POT. 2' unidades formadoras de colonias de la cepa B. abortus 2308 virulenta. El analisis global de los resultados demostro una proteccion del 11.9 por ciento en animales vacunados con el extracto de fenol insoluble, 65 por ciento en los vacunados con B. abortus cepa 19 y 95 por ciento en el grupo que recibio vacuna B. melitensis Rev. 1.
Assuntos
Animais , Humanos , Masculino , Feminino , Cobaias , Antígenos de Bactérias/imunologia , Brucella abortus/imunologia , Vacina contra Brucelose/imunologia , Brucelose/prevenção & controle , Peso Corporal , Brucella abortus/patogenicidade , Brucella melitensis/imunologia , Brucelose/imunologia , Fenóis , VirulênciaRESUMO
A phenol insoluble extract vaccine proposed to immunize men against brucellosis was tested for its ability in protecting guinea pigs against challenge with virulent Brucella abortus strain 2308. Living attenuated Brucella abortus strain 19 and B. melitensis Rev. 1 were included in the experiment for comparison. Ninety three animals were vaccinated in each group and subdivided in subgroups of 31 for challenge with 10(4), 10(3) and 10(2) colony forming units of virulent B. abortus 2308. A global analysis of the results showed protection of 11.9%, 65% and 95% in animals vaccinated with phenol insoluble extract, strain 19 and Rev. 1, respectively.
Assuntos
Antígenos de Bactérias/imunologia , Vacina contra Brucelose/imunologia , Brucella abortus/imunologia , Brucelose/prevenção & controle , Animais , Peso Corporal , Brucella abortus/patogenicidade , Brucella melitensis/imunologia , Brucelose/imunologia , Feminino , Cobaias , Humanos , Masculino , Fenóis , VirulênciaRESUMO
The use of diagnostic tests for the selection of healthy individual persons or animals for inclusion in certain groups carries with it an inherent risk of including those with infections because they falsely reacted negatively. The factors of sensitivity of the test, prevalence of the disease, and the size of the group all influence the degree of such risk. The probability of including an infected individual person or animal can be calculated combining conditional probability and the binomial distribution.
Assuntos
Reações Falso-Negativas , Infecções/epidemiologia , Animais , Brucelose Bovina/diagnóstico , Brucelose Bovina/epidemiologia , Bovinos , Humanos , Infecções/diagnóstico , Valor Preditivo dos Testes , ProbabilidadeRESUMO
Ten stabilizing media commonly used by commercial laboratories for the stabilization of Brucella abortus strain 19 for lyophilization were studied. Six media gave similar results, with viable count averages ranging from 50% to 58%. The standard stabilizer recommended by the World Health Organization (casitone 2,5%, sucrose 5% and sodium glutamate 1%) was included. The other four media gave viabilities with averages from 19,7% to 38,7%, which represented a highly significant difference. The lowest viable count was observed with the 5% polyvinylpyrrolidone stabilizer. The differences in the number of viable cells among the various processes were greater than expected.
Assuntos
Vacina contra Brucelose/normas , Brucella abortus/crescimento & desenvolvimento , Liofilização , Veículos Farmacêuticos/farmacologia , Brucella abortus/imunologia , Contagem de Células , Sobrevivência Celular/efeitos dos fármacos , Povidona/farmacologiaRESUMO
Ten stabilizing media commonly used by commercial laboratories for the stabilization of Brucella abortus strain 19 for lyophilization were studied. Six media gave similar results, with viable count averages ranging from 50
to 58
. The standard stabilizer recommended by the World Health Organization (casitone 2,5
, sucrose 5
and sodium glutamate 1
) was included. The other four media gave viabilities with averages from 19,7
to 38,7
, which represented a highly significant difference. The lowest viable count was observed with the 5
polyvinylpyrrolidone stabilizer. The differences in the number of viable cells among the various processes were greater than expected.
RESUMO
Hemocultures and myelocultures of samples obtained from the ilium crest were made simultaneously of 61 patients with serologic diagnosis of brucellosis. Five strains were isolated by both methods and 18, by myeloculture only. All samples from which Brucella were isolated by hemoculture were also positive by myeloculture. The advantages of making myelocultures of patients previously treated with antibiotics are emphasized.
Assuntos
Medula Óssea/microbiologia , Brucella/isolamento & purificação , Brucelose/microbiologia , Adulto , Brucella/crescimento & desenvolvimento , Brucella abortus/isolamento & purificação , Humanos , Pessoa de Meia-Idade , Testes SorológicosRESUMO
Hemocultures and myelocultures of samples obtained from the ilium crest were made simultaneously of 61 patients with serologic diagnosis of brucellosis. Five strains were isolated by both methods and 18, by myeloculture only. All samples from which Brucella were isolated by hemoculture were also positive by myeloculture. The advantages of making myelocultures of patients previously treated with antibiotics are emphasized.
RESUMO
Hemocultures and myelocultures of samples obtained from the ilium crest were made simultaneously of 61 patients with serologic diagnosis of brucellosis. Five strains were isolated by both methods and 18, by myeloculture only. All samples from which Brucella were isolated by hemoculture were also positive by myeloculture. The advantages of making myelocultures of patients previously treated with antibiotics are emphasized.