RESUMO

The effect of a natural sesquiterpene ketone, 9,10-dehydrofukinone (DHF), on pathogenic Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from chronic infectious processes, was the focus of the present study. Lipophilic DHF produced important antibacterial synergistic effects in association with ciprofloxacin (CPX) against two biofilm-forming strains of S. aureus HT1 (FIC=0.21) and P. aeruginosa HT5 (FIC=0.05). Hence, this mixture constitutes an excellent strategy to combat these biofilm-producing bacteria that overexpress drug efflux pumps as a resistance mechanism. Additionally, a substantial rise in beneficial Lactobacillus biofilm biomass was determined as a very significant finding of this association. Particularly, a non-pathogenic biofilm increment of 119 % was quantified when the mixture was added to a probiotic L. acidophilus ATCC SD-5212 culture. A surface activity enhanced in 71 % with respect to untreated L. acidophilus culture was also generated by the DHF and CPX association, and therefore, a glycoprotein synthesis induction mediated by the mixture is discussed. The results obtained could help in the development of new selective antibiotics. From an ecological standpoint, the present study strongly suggests that DHF is a polyfunctional organic molecule produced with a high yield in Senecio punae that exerts a positive impact on a non-pathogenic plant bacterium L. plantarum CE105.

Assuntos

RESUMO

Objetivo: Se describió la variación de la adherencia de las Guías de Práctica Clínica (GPC), luego de la implementación del módulo "terapéutica sugerida" en el sistema informático asistencial de un establecimiento de salud privado en La Libertad. Material y métodos: Se comparó el nivel de adherencia trimestral de las GPC antes y después de la intervención, mediante auditoría de la información asistencial. Resultados: El nivel de adherencia de las GPC antes de las modificaciones estuvo entre 63% y 65%, observándose un incremento hasta 75% posterior a la implementación del módulo "terapéutica sugerida". Conclusión: La implementación de módulos facilitadores de la acción asistencial en el sistema informático podría mejorar la adhesión a las GPC, lo que sugiere la necesidad de replicar otros estudios

Objective: The variation of the adhesion of the Guidelines of Clinical Practice (GPC), after the implementation of the "suggested therapeutic" module in the computer system of a private health facility in La Libertad was described. Material and methods: The level of quarterly adherence of GPC was compared before and after the intervention, by an audit of healthcare information. Results: The level of adherence of GPC before the amendments was between 63% and 65%, with an increase to 75% after the implementation of the "suggested therapeutic" module. Conclusion: Use of facilitator's modules of healthcare assistance in the information system could improve adherence to GPC, which suggest the need to replicate other studies

RESUMO

Understanding the molecular underpinnings involved in the reproduction of the salmon louse is critical for designing novel strategies of pest management for this ectoparasite. However, genomic information on sex-related genes is still limited. In the present work, sex-specific gene transcription was revealed in the salmon louse Caligus rogercresseyi using high-throughput Illumina sequencing. A total of 30,191,914 and 32,292,250 high quality reads were generated for females and males, and these were de novo assembled into 32,173 and 38,177 contigs, respectively. Gene ontology analysis showed a pattern of higher expression in the female as compared to the male transcriptome. Based on our sequence analysis and known sex-related proteins, several genes putatively involved in sex differentiation, including Dmrt3, FOXL2, VASA, and FEM1, and other potentially significant candidate genes in C. rogercresseyi, were identified for the first time. In addition, the occurrence of SNPs in several differentially expressed contigs annotating for sex-related genes was found. This transcriptome dataset provides a useful resource for future functional analyses, opening new opportunities for sea lice pest control.

Assuntos

RESUMO

The California red abalone, Haliotis rufescens that belongs to the Haliotidae family, is the largest species of abalone in the world that has sustained the major fishery and aquaculture production in the USA and Mexico. This native mollusk has not been evaluated or assigned a conservation category even though in the last few decades it was heavily exploited until it disappeared in some areas along the California coast. In Chile, the red abalone was introduced in the 1970s from California wild abalone stocks for the purposes of aquaculture. Considering the number of years that the red abalone has been cultivated in Chile crucial genetic information is scarce and critical issues remain unresolved. This study reports and validates novel single nucleotide polymorphisms (SNP) markers for the red abalone H. rufescens using cDNA pyrosequencing. A total of 622 high quality SNPs were identified in 146 sequences with an estimated frequency of 1 SNP each 1000bp. Forty-five SNPs markers with functional information for gene ontology were selected. Of these, 8 were polymorphic among the individuals screened: Heat shock protein 70 (HSP70), vitellogenin (VTG), lysin, alginate lyase enzyme (AL), Glucose-regulated protein 94 (GRP94), fructose-bisphosphate aldolase (FBA), sulfatase 1A precursor (S1AP) and ornithine decarboxylase antizyme (ODC). Two additional sequences were also identified with polymorphisms but no similarities with known proteins were achieved. To validate the putative SNP markers, High Resolution Melting Analysis (HRMA) was conducted in a wild and hatchery-bred population. Additionally, SNP cross-amplifications were tested in two further native abalone species, Haliotis fulgens and Haliotis corrugata. This study provides novel candidate genes that could be used to evaluate loss of genetic diversity due to hatchery selection or inbreeding effects.

Assuntos

RESUMO

La actividad del transportador de colina de alta afinidad (HAChT) es considerado el paso limitante en la síntesis de acetilcolina (ACh) en el terminal colinérgico. Estudios recientes muestran que el HAChT contiene residuos de serina y treonina consensuales para la fosforilación por proteína kinasa A (PKA). Usando neuronas de retina de embrión de pollo se evaluó el efecto del segundo mensajero AMPc sobre la actividad del HAChT. El aumento de los niveles intracelulares de AMPc a través de la inhibición de la fosfodiesterasa, activación de la adenilato ciclasa o usando un análogo de AMPc resistente a la fosfodiesterasa disminuyó la actividad del HAChT entre 29 y 69%. Por otra parte, la activación de receptores de dopamina tipo-D1 aumenta los niveles de AMPc intracelular y activa PKA, sin embargo, el tratamiento con dopamina o con antagonistas de los receptores dopaminergicos D1 o D2 no induce cambios en la actividad del transportador

The high affinity choline transporter (HAChT) activity is considered to be the rate-limiting step in acetylcholine (ACh) synthesis in the cholinergic terminal. Recent studies show that HAChT contains consensus serine and threonine residues for protein kinase A (PKA) phosphorylation. Using chick retinal neurons evaluated the effects of the second messenger cAMP on the HAChT activity. The increase of the intracellular cAMP levels through phosphodiesterase inhibition, adenilatecyclase activation or using a phosphodiesterase-resistant cAMP analog decreased HAChT activity between 29 and 69%. Moreover, the activation of dopamine D1-type receptors increase the intracellular cAMP levels and activates PKA, however, the treatment with dopamine D1 or D2 receptor antagonists does not induce changes on transporter activity

Assuntos

RESUMO

OBJETIVOS. Describir las características histológicas de las vellosidades coriales de embarazos ectópicos. DISEÑO. Estudio anatomopatológico descriptivo, retrospectivo. LUGAR. Departamento de Patología, Instituto Nacional Materno Perinatal (INMP). MATERIAL BIOLÓGICO. Vellosidades coriales de embarazos ectópicos. INTERVENCIONES. Se describe las características histológicas de las vellosidades coriales de una serie de 98 embarazos ectópicos consecutivos, de los archivos del Departamento de Patología del INMP. PRINCIPALES MEDIDAS DE RESULTADOS. Patrones histológicos de las vellosidades coriales. RESULTADOS. Se describe cinco patrones histológicos de las vellosidades coriales. CONCLUSIONES. Se señala la posibilidad que los cambios observados sean expresión de patología materno-fetal.

OBJECTIVES. To describe ectopic pregnancy chorion villi histological patterns. DESIGN. Descriptive, retrospective anatomic and pathologic study. SETTING. Pathology Department, Maternal Perinatal National Institute, Lima, Peru (INMP). BIOLOGIC MATERIAL. Ectopic pregnancies chorion villi. INTERVENTIONS. The histological characteristics of a series of 98 ectopic pregnancies from the files of the Department of Pathology were studied. MAIN OUTCOME MEASURES. Histological patterns of chorion villi. RESULTS. We describe five different histological patterns of chorial villi. CONCLUSIONS. The possibility that these chorial villi histology changes are related to materno-fetal pathology is considered. OBJECTIVES. To describe ectopic pregnancy chorion.

Assuntos

RESUMO

BACKGROUND: Benzodiazepine use is associated to a risk of abuse of dependency. In Chile benzodiazepine abuse is a public health issue. In an attempt to overcome this problem, several restrictions in their prescription were implemented by the health authority. Despite these measures, inadequate use of benzodiazepines in primary care continues to be frequent. AIM: To study the frequency of benzodiazepine use among primary care patients. MATERIAL AND METHODS: Cross sectional survey done during two days, with seven physicians working in public primary care clinics. They applied a structured questionnaire about psychotropic medication use. RESULTS: One hundred eighty eight subjects aged 46.8 +/- 17.5 years, 66% women, were interviewed. Thirty two percent were consuming a psychotropic drug and 82% of these corresponded to benzodiazepines. General practitioners and psychiatrists prescribed the drug to 75% and 13% of subjects, respectively. A higher frequency of benzodiazepine use was observed in women and in urban areas. CONCLUSIONS: Benzodiazepine use continues to be highly prevalent in primary care patients, in spite of the nationwide implementation of controlled prescriptions.

Assuntos

RESUMO

Lymphocele can develop after renal transplantation. Surgical internal drainage to the abdominal cavity through a standard laparotomy is indicated for symptomatic persistent lesions. Internal drainage can be performed laparoscopically. In this report, we describe our laparoscopic surgical technique for drainage and analyze our results in five patients with lymphoceles that developed after kidney transplantation. All the patients were male, with a mean age of 29 +/- 10 years. The volume of fluid in the lymphoceles ranged from 500 to 1000 mL. Percutaneous drainage was selected as the initial treatment without success. A laparoscopic peritoneal window was created in all patients under intraoperative ultrasonographic guidance. The mean operative time was 90 minutes. In all patients, the fluid collections resolved after laparoscopic internal drainage without complications. During a mean follow-up of 3 +/- 2 years, all patients remained asymptomatic, and no additional fluid collections were identified.

Assuntos

RESUMO

Patients hospitalized with community acquired pneumonia were studied prospectively in two hospitals located in the surroundings of Buenos Aires city. Fifty two patients from General Hospital Manuel Belgrano (HMB) were included from March 1998 to February 1999 and 23 patients from Hospital Dr A. Cetrangolo (HCET) for respiratory disease, were included from June 2000 to May 2001. Patients with lung tuberculosis, lung neoplasia and HIV infection were excluded. Clinical background, signs and symptoms were recorded. Microbiological examinations performed included bacteria, respiratory viruses and mycobacteria. Studies for "atypical" bacteria (Chlamydia spp., Coxiella burnetii, Mycoplasma pneumoniae and Legionella spp.) were carried out by serological methods. No differences in age and gender were observed between both groups. Most frequently observed comorbidities in the HMB group included COPD, diabetes and cardiac failure while in the HCET group these were COPD, asthma and lung fibrosis. Etiology was established in 48% and 65.2% of the patients in the first and second group, respectively. Most frequent agents were Mycoplasma pneumoniae, Streptococcus pneumoniae, influenza A and Legionella spp.; the last one was detected in 12% of the patients. Most of these patients were from HMB and presented a good outcome. Mortality was similar in both groups (13.3%). In the HBM group it was related to the presence of comorbidities in 7 out of 8 cases, and in the HCET group it was a consequence of the worsening of their chronic respiratory failure.

Assuntos

RESUMO

Patients hospitalized with community acquired pneumonia were studied prospectively in two hospitals located in the surroundings of Buenos Aires city. Fifty two patients from General Hospital Manuel Belgrano (HMB) were included from March 1998 to February 1999 and 23 patients from Hospital Dr A. Cetrangolo (HCET) for respiratory disease, were included from June 2000 to May 2001. Patients with lung tuberculosis, lung neoplasia and HIV infection were excluded. Clinical background, signs and symptoms were recorded. Microbiological examinations performed included bacteria, respiratory viruses and mycobacteria. Studies for [quot ]atypical[quot ] bacteria (Chlamydia spp., Coxiella burnetii, Mycoplasma pneumoniae and Legionella spp.) were carried out by serological methods. No differences in age and gender were observed between both groups. Most frequently observed comorbidities in the HMB group included COPD, diabetes and cardiac failure while in the HCET group these were COPD, asthma and lung fibrosis. Etiology was established in 48

and 65.2

of the patients in the first and second group, respectively. Most frequent agents were Mycoplasma pneumoniae, Streptococcus pneumoniae, influenza A and Legionella spp.; the last one was detected in 12

of the patients. Most of these patients were from HMB and presented a good outcome. Mortality was similar in both groups (13.3

). In the HBM group it was related to the presence of comorbidities in 7 out of 8 cases, and in the HCET group it was a consequence of the worsening of their chronic respiratory failure.

RESUMO

Patients hospitalized with community acquired pneumonia were studied prospectively in two hospitals located in the surroundings of Buenos Aires city. Fifty two patients from General Hospital Manuel Belgrano (HMB) were included from March 1998 to February 1999 and 23 patients from Hospital Dr A. Cetrangolo (HCET) for respiratory disease, were included from June 2000 to May 2001. Patients with lung tuberculosis, lung neoplasia and HIV infection were excluded. Clinical background, signs and symptoms were recorded. Microbiological examinations performed included bacteria, respiratory viruses and mycobacteria. Studies for "atypical" bacteria (Chlamydia spp., Coxiella burnetii, Mycoplasma pneumoniae and Legionella spp.) were carried out by serological methods. No differences in age and gender were observed between both groups. Most frequently observed comorbidities in the HMB group included COPD, diabetes and cardiac failure while in the HCET group these were COPD, asthma and lung fibrosis. Etiology was established in 48% and 65.2% of the patients in the first and second group, respectively. Most frequent agents were Mycoplasma pneumoniae, Streptococcus pneumoniae, influenza A and Legionella spp.; the last one was detected in 12% of the patients. Most of these patients were from HMB and presented a good outcome. Mortality was similar in both groups (13.3%). In the HBM group it was related to the presence of comorbidities in 7 out of 8 cases, and in the HCET group it was a consequence of the worsening of their chronic respiratory failure (AU)

Assuntos

RESUMO

Patients hospitalized with community acquired pneumonia were studied prospectively in two hospitals located in the surroundings of Buenos Aires city. Fifty two patients from General Hospital Manuel Belgrano (HMB) were included from March 1998 to February 1999 and 23 patients from Hospital Dr A. Cetrangolo (HCET) for respiratory disease, were included from June 2000 to May 2001. Patients with lung tuberculosis, lung neoplasia and HIV infection were excluded. Clinical background, signs and symptoms were recorded. Microbiological examinations performed included bacteria, respiratory viruses and mycobacteria. Studies for "atypical" bacteria (Chlamydia spp., Coxiella burnetii, Mycoplasma pneumoniae and Legionella spp.) were carried out by serological methods. No differences in age and gender were observed between both groups. Most frequently observed comorbidities in the HMB group included COPD, diabetes and cardiac failure while in the HCET group these were COPD, asthma and lung fibrosis. Etiology was established in 48% and 65.2% of the patients in the first and second group, respectively. Most frequent agents were Mycoplasma pneumoniae, Streptococcus pneumoniae, influenza A and Legionella spp.; the last one was detected in 12% of the patients. Most of these patients were from HMB and presented a good outcome. Mortality was similar in both groups (13.3%). In the HBM group it was related to the presence of comorbidities in 7 out of 8 cases, and in the HCET group it was a consequence of the worsening of their chronic respiratory failure

Assuntos

RESUMO

Veinte pacientes con diagnóstico de luxación recidivante anterior de hombro fueron tratados con una nueva técnica quirúrgica de capsuloplastia, el cual consistió en imbricación de la cápsula articular y fijación con dos tornillos de esponjosa de 3,5mm de diámetro modificados con una perforación transversal en la cabeza, por la cual se pasa una sutura de Vicry- Nº1 (tornillo modificado de fraute). Los pacientes fueron evaluados previo y posterior a la cirugía para determinar rango de movilidad articular, inestabilidad y fuerza muscular. Los resultados fueron comparados con el hombro contra lateral. La abducción y rotación externa disminuyeron una media de 15º (p<0.001) y 3,06º (p<0.0001) respectivamente, en relación al hombro contralateral. Diecisiete pacientes presentaron resultados catalogados como buenos o excelentes. Creemos que nuestro tornillo es una alternativa para solucionar la inestabilidad anterior al hombro

Assuntos

RESUMO

El presente trabajo expone las coincidencias y diferencias diagnósticas en pacientes que consultaron el Hospital Vargas de Caracas, uno con diagnóstico de osteomielitis crónica y el otro con un osteosarcoma; los cuales resultaron ser un osteosarcoma osteoide y una osteomielitis crónica inespecífica respectivamente. El laboratorio de rutina y la radiología simple no permitió distinguir entre esas entidades; y el diagnóstico definitivo se hizo por biopsia y análisis histopatológico. La estrategia diagnóstica dependerá de la posibilidad del médico de realizar un diagnóstico diferencial acertado en base a la información clínica y estudios radiológicos

Assuntos

RESUMO

En este trabajo se presenta el caso de un recién nacido con neoplasia endocrina múltiple, poniéndose énfasis en las diferencias entre los síndromes clásicos y el que ahora se describe. También se comenta la naturaleza de las lesiones proliferativas.

Assuntos

RESUMO

La presente publicación describe un nuevo enfoque que analiza integralmente los aspectos clínicos con los hallazgos de laboratorio y anatomo patológicos, en dos momentos diferentes, pero con el objetivo de mejorar la calidad de atención brindada en los establecimientos de salud(AU)

Assuntos

RESUMO

La presente publicación describe un nuevo enfoque que analiza integralmente los aspectos clínicos con los hallazgos de laboratorio y anatomo patológicos, en dos momentos diferentes, pero con el objetivo de mejorar la calidad de atención brindada en los establecimientos de salud

Assuntos

RESUMO

El nuevo Régimen de Seguridad en Salud, puesto en vigencia con la promulgación de la Ley 100 de 23 de Diciembre de 1.993, demanda que los recursos e infraestructura existentes para la prestación de servicios de salud, tengan un adecuado aprovechamiento que permita el acceso de la población a todos los niveles de atención.-En esta propuesta se hace un diagnostico de la situación de salud del Departamento del Quindío, en donde se tiene en cuenta la morbilidad, la mortalidad y la infraestructura institucional y de talento humano con el que se cuenta actualmente.Se analizan además aspectos determinantes de la din mica cultural y económica de la región, reseñando determinantes estructurales políticos, demográficos y de influencia externa.-Se analiza el estado de la oferta y la demanda y su posición frente al Sistema de Seguridad Social en Salud del País (Régimen Subsidiado, vinculado y contributivo).Por último, se pone en consideración una propuesta de Red de Servicios de Salud para el Departamento, donde se esquematizan estrategias para el desarrollo de la Red, como también las actividades necesarias para el desarrollo de las estrategias propuestas...

Assuntos

RESUMO

Con el fin de comparar la eficacia antipirética del ibuprofeno y del acetaminofeno, se estudiaron ochenta y tres (83) pacientes en edades comprendidas entre 3 meses y 12 años, con enfermedad aguda intermitente febril, que acudieron a Consulta de Emergencia del Hospital "Rafael Antomnio Gil" de Duaca o a la Consulta Privada de la Clínica Infantil Santa Cruz de Barquisimeto, desde enero a marzo de 1994. Estos pacientes fueron distribuidos en dos grupos: uno de 42 pacientes y otro de 41. Al primero se le admisnitró acetaminofeno en dosis de 10 mg/kg/dosis y al otro ibuprofeno a 10 mg/kg/dosis. Se determinó la temperatura rectal previa a la admisnitración del medicamento y una hora después. Los dos medicamentos empleados produjeron descenso de la temperatura. El descenso de la temperatura fue mayor con el ibuprofeno. No se registraron efectos adversos en ningún paciente. Concluimos que el ibuprofeno, adminsitrado por vía oral, produce un mayor descenso de la temperatura, alcabo de una hora y a la dosis indicada, en niños de 1 a 12 años

Assuntos

RESUMO

Se recopilan 9 casos de tuberculosis congenita, que cumplen con los postulados de Beitzke, resultado de una encuesta efectuada en los hospitales de Lima que cuentan con Servicios de Neonatología, de una revisión de la literatura médica nacional y del aporte de 2 recién nacidos en el Hospital E. Rebagliati. 3 de los 9 casos encontrados correspondieron a la forma de infección congenita producida por diseminación hematógena placentaria, y los otros 6 a la adquirida por aspiración de secreciones infectadas durante el parto. Todos ellos fueron Recien Nacidos de Bajo Peso (menos de 2500 gr). La clínica fue variable pero con frecuencia se presentaron fiebre, falta de ganancia de peso, hepatomegalia y cuadro respiratorio. La mortalidad ocurrió en 7 de los 9 pacientes y el diagnostico se hizo con la necropsia. Los 2 casos sobrevivientes recibieron multiples drogas antituberculosas, hecho que confirma la falta de información para recomendar un régimen preferido de tratamiento, agregado a la administración de Izoniazida. El diagnóstico precoz y el tratamiento adecuado son los elementos cardinales para disminuir la elevada mortalidad de la tuberculosis congenita.

Assuntos