RESUMO
BACKGROUND: Aldehyde dehydrogenase isoform 1 (ALDH1) has been shown to be a marker of cancer stem cells (CSCs). These stem cells may be responsible for tumour perpetuation as well as local and distant invasion. Several studies have shown that CSCs are more chemoradiotherapy (CRT)-resistant and may be responsible for tumour recurrence. Other studies, in contrast, have found ALDH1 expression to be indicative of a better prognosis. METHODS: We retrospectively evaluated 84 patients diagnosed and treated for laryngeal cancer between 2006 and 2011. All patients underwent curative-intent radiotherapy or CRT at our institution. 57 of the 84 tumour samples contained sufficient material for ALDH1 assessment. RESULTS: ALDH1 expression was detected in 17.5 % (10/57) of the tissue samples. None of the tumours from stage I patients tested positive for ALDH1. The relapse rate in ALDH1 + patients was 10 versus 51.2 % for ALDH1-. No differences in overall survival were observed between the groups; however, disease-free survival was 90 % for the ALDH1 + group versus 48.9 % for ALDH1- patients (p = 0.034). CONCLUSION: The patients in this study with ALDH1 + tumours had better outcomes than their counterparts with ALDH1- tumours. This finding suggests that not all CSCs are resistant to conventional cancer treatments. It may also imply that new methods of correctly identifying these cells are needed.
Assuntos
Biomarcadores Tumorais/análise , Isoenzimas/biossíntese , Neoplasias Laríngeas/patologia , Tolerância a Radiação/fisiologia , Retinal Desidrogenase/biossíntese , Adulto , Idoso , Idoso de 80 Anos ou mais , Família Aldeído Desidrogenase 1 , Intervalo Livre de Doença , Feminino , Humanos , Imuno-Histoquímica , Isoenzimas/análise , Estimativa de Kaplan-Meier , Neoplasias Laríngeas/enzimologia , Neoplasias Laríngeas/mortalidade , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/enzimologia , Recidiva Local de Neoplasia/mortalidade , Recidiva Local de Neoplasia/patologia , Células-Tronco Neoplásicas/enzimologia , Células-Tronco Neoplásicas/patologia , Prognóstico , Modelos de Riscos Proporcionais , Retinal Desidrogenase/análise , Estudos RetrospectivosRESUMO
PURPOSE: Aldehyde dehydrogenase enzymes are a family of intracellular enzymes that participate in cellular detoxification, differentiation and drug resistance through the oxidation of cellular aldehydes. The isoform 1 (ALDH1) has been proved useful for the identification of cancer stem cells. The ALDH1 cytoplasmatic expression has been associated with poor prognostis in several tumours, such as non-small cell lung cancer. The role of the ALDH1 nuclear expression remains unknown. METHODS: We conducted a historical cohort study in 89 patients diagnosed of stage I non-small cell lung cancer treated with surgery between 2009 and 2004 in the Thoracic Surgery Department in the Universitary Hospital Puerta de Hierro. We selected from this sample those cases with nuclear expression of the ALDH1. RESULTS: Three of the 89 (3.3 %) patients showed a nuclear expression of the ALDH1. The three of them are still alive with a median time of follow up of 73 months (more than 6 years). CONCLUSION: We have identified ALDH1 as a nuclear protein in early stage non-small cell lung cancer. It might have a function in cell cycle control, associating a better prognosis to these patients. More studies are necessary to clarify the role of nuclear expression of ALDH1.
Assuntos
Adenocarcinoma/enzimologia , Carcinoma Mucoepidermoide/enzimologia , Carcinoma Pulmonar de Células não Pequenas/enzimologia , Carcinoma de Células Escamosas/enzimologia , Núcleo Celular/enzimologia , Isoenzimas/metabolismo , Neoplasias Pulmonares/enzimologia , Células-Tronco Neoplásicas/enzimologia , Retinal Desidrogenase/metabolismo , Adenocarcinoma/patologia , Adulto , Idoso , Família Aldeído Desidrogenase 1 , Carcinoma Mucoepidermoide/patologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma de Células Escamosas/patologia , Estudos de Coortes , Humanos , Neoplasias Pulmonares/patologia , Masculino , Estadiamento de Neoplasias , Prognóstico , Estudos RetrospectivosRESUMO
BACKGROUND: HIF-1alpha plays a key role in the development and progression of cancer. Its polymorphic variants C1772T and G1790A have been associated with greater susceptibility to cancer and increased tumor progression. METHODS: We determined the distribution of these polymorphisms among 121 patients with glottic cancer and 154 healthy volunteers by PCR-RFLP. We also analyzed the relationship between the presence of these polymorphisms and various clinicopathologic variables. RESULTS: Advanced tumors (T3-T4) were associated with the TT variant (p = 0.036), which was present in 75 % of T4 tumors (p = 0.008). Among patients with nodal metastasis (N+), 41.7 and 22 % were carrying the TT and GA variants, respectively, compared with 9.4 and 2 % of the patients with no metastasis (N0), (p = 0.006 and p = 0.032). CONCLUSIONS: The presence of the TT and GA variants were associated with lymph node metastasis, while the presence of the TT variant can be associated with larger tumor size.
Assuntos
Carcinoma de Células Escamosas/genética , Glote/patologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Neoplasias Laríngeas/genética , Polimorfismo Genético , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/patologia , Feminino , Genótipo , Humanos , Neoplasias Laríngeas/patologia , Metástase Linfática , Masculino , Pessoa de Meia-IdadeRESUMO
A Colombian strain of Trypanosoma rangeli was characterized by analyzing its behaviour in different axenic and cellular culture, its infection rate and the histopathological lesions produced in experimental animals. Although slight inflammatory infiltrations were shown in different histopathological sections, no pseudocysts could be observed. Grace's insect medium is better than liver infusion tryptose or artificial triatomine urine supplemented with proline when studying T. rangeli metacyclogenesis, with a peak of 32 per cent trypomastigotes. High infection rates were found in VERO and J774 cells. Because of its 100 per cent infectivity rates and adequacy of parasitemia levels, C23 strain is a suitable model of T. rangeli biology study.
Assuntos
Animais , Infecções/parasitologia , Trypanosoma/isolamento & purificação , Técnicas de Cultura de CélulasRESUMO
A Colombian strain of Trypanosoma rangeli was characterized by analyzing its behaviour in different axenic and cellular culture, its infection rate and the histopathological lesions produced in experimental animals. Although slight inflammatory infiltrations were shown in different histopathological sections, no pseudocysts could be observed. Grace's insect medium is better than liver infusion tryptose or artificial triatomine urine supplemented with proline when studying T. rangeli metacyclogenesis, with a peak of 32% trypomastigotes. High infection rates were found in VERO and J774 cells. Because of its 100% infectivity rates and adequacy of parasitemia levels, C23 strain is a suitable model of T. rangeli biology study.
Assuntos
Trypanosoma/isolamento & purificação , Animais , Colômbia , Camundongos/parasitologiaRESUMO
Knowing the great diversity of medical and biological properties of Trypanosoma cruzi, the causal agent of Chagas' disease, we quantified the morphological parameters that typify the different forms of three clones of T. cruzi and their original strain, Bolivia, in comparison among themselves and with strain Bolivia, attempting to provide additional data concerning the clonal biological behaviour of this parasite. Blood forms morphology was quantified using a computerized image analysis (Videoplan/Kontron) and statistical analysis was determined using ANOVA-1 Test. Large number of quantitative differences among slender, broad, and stout forms were found. The comparison of clones I, II and III with their mother strain, leads to the emergence of significant differences in at least 12 parameters out of the 16 we studied. When clones were compared among themselves, the differences decreased. Variations of the percentages of the three kinds of clones were found along the acute infection. These data are the first step in correlating the morphological and pathogenic characteristics of the parasite.
Assuntos
Clonagem de Organismos , Trypanosoma cruzi/citologia , Animais , Bolívia , Doença de Chagas/sangue , Processamento de Imagem Assistida por Computador , Masculino , Camundongos , Trypanosoma cruzi/genética , Trypanosoma cruzi/patogenicidadeRESUMO
After isolating three clones of Trypanosoma cruzi (Bolivia), we first characterized them according to parasitaemia, pleomorphism and virulence, and then histopathologically. The study's interest lies on the hypothesis that clonal evolution of T. cruzi has a major impact on biologically relevant properties of this parasite. Data obtained from the studies of parasitaemia, pleomorphism and virulence showed no differences between the groups studied. As a final point, the histopathological study shows us a muscular tissue tropism both in clones and in their mother strain (Bolivia). In this paper, we conclude that Bolivia strain and clones isolated from it, pertaining to the same major clone share similar biological properties.
Assuntos
Animais , Masculino , Camundongos , Trypanosoma cruzi , Células Clonais , Heterogeneidade Genética , Fibras Musculares Esqueléticas , Músculo Esquelético/patologia , Miocárdio , Parasitemia , Trypanosoma cruzi , VirulênciaRESUMO
After isolating three clones of Trypanosoma cruzi (Bolivia), we first characterized them according to parasitaemia, pleomorphism and virulence, and then histopathologically. The study's interest lies on the hypothesis that clonal evolution of T. cruzi has a major impact on biologically relevant properties of this parasite. Data obtained from the studies of parasitaemia, pleomorphism and virulence showed no differences between the groups studied. As a final point, the histopathological study shows us a muscular tissue tropism both in clones and in their mother strain (Bolivia). In this paper, we conclude that Bolivia strain and clones isolated from it, pertaining to the same major clone share similar biological properties.
Assuntos
Trypanosoma cruzi/genética , Animais , Células Clonais , Heterogeneidade Genética , Masculino , Camundongos , Fibras Musculares Esqueléticas/patologia , Músculo Esquelético/patologia , Miocárdio/patologia , Parasitemia , Trypanosoma cruzi/citologia , Trypanosoma cruzi/patogenicidade , VirulênciaRESUMO
After morphological and histopathological characterization, the Cali strain of T. cruzi isolated from a patient in Cali (Colombia), was also submitted to a morphometrical characterization. For this we estimated 16 cellular parameters in 30 samples of each evolutive form of T. cruzi in blood in NNN medium through planimetry by the IBAS-1 (Image Basic Analytic System). This morphometrical study was completed with a statistical one through the application of the variance (ANOVA-1). This made it possible to evidence a great number of significant differences between the slender, broad and stout forms and also between the short and large epimastigotes from the NNN medium. These data have a real interest for classifying some features of the biological cycle of T. cruzi as well as the future development of diagnosis techniques or therapeutical and prophylactic experiments.