RESUMO

Acid protease production by a local strain of Mucor bacilliformis was performed by solid state cultivation on different agricultural by-products as substrate. The effects of different parameters on enzyme biosynthesis were studied: Wheat bran wetted at 120% with a 200 mM HCl solution and inoculated with 5 x 10(5) spores/g produced a milk clotting activity of 7500 U/g bran after 72 h cultivation at 24 degrees C.

Assuntos

Ácido Aspártico Endopeptidases/biossíntese , Proteínas Fúngicas/biossíntese , Mucor/enzimologia , Ácido Aspártico Endopeptidases/genética , Meios de Cultura , Fibras na Dieta , Fermentação , Proteínas Fúngicas/genética , Concentração de Íons de Hidrogênio , Cinética , Proteínas do Leite/metabolismo , Micologia/métodos , Oryza , Extratos Vegetais , Sementes , Temperatura , Água