RESUMO
Lachesis muta snake venom induced aggregation of bromelain sensitized human erythrocytes at a concentration of 1 mg/ml. The hemagglutinating protein was purified by DEAE-Sephadex A-50 column chromatography. Polyacrylamide gel electrophoresis revealed at least three bands, whereas SDS electrophoresis in the presence of 2-mercaptoethanol showed a single one. Isoelectric focusing revealed hemagglutinating activity in the range of pH 3-8. The maximum peak (mutina) at pH 5.5. This fraction was active in agglutinating human RBC of types A, B, O Rh (+) and B, O Rh (-). One mM EDTA and 1 mM Ca++ did not alter the agglutinating time significantly. Lactose and inositol inhibited the agglutination of A, B, O Rh (+) and B, O Rh (-) human RBC. The present study showed the non specificity of the hemagglutinating activity of mutina. It was also shown that mutina is a non-mitogenic protein.
Assuntos
Venenos de Crotalídeos/análise , Lectinas/isolamento & purificação , Aglutinação , Animais , Fracionamento Químico , Eritrócitos/imunologia , Testes de Hemaglutinação , Agregação PlaquetáriaRESUMO
La accion citolitica de sustancias simples y de agentes biologicos complejos puede ser descrita por ecuaciones tipo Hill. Se propone la medicion del poder hemolitico de venenos de serpientes en terminos de la disminucion de la accion litica en una dilucion 1:10. La ventaja de este procedimiento es que el poder citolitico es una funcion de las propriedades bioquimicas del sistema, mas que de la concentracion de los principios activos. Se muestran los poderes hemoliticos de algunos venenos de serpientes sobre eritrocitos humanos O Rh(+)