RESUMO

BACKGROUND: Obesity, dyslipidemia, and low-grade inflammatory state form a triad of self-sustaining metabolic dysfunction. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy is a simple, rapid, and non-destructive technique that generates spectral fingerprints of biomolecules that can be correlated with metabolic changes. We verified the efficiency of ATR-FTIR spectroscopy in blood plasma (n = 74) to discriminate the types of dyslipidemias and suggest metabolic inflammatory changes. METHODS: Principal Component Analysis (PCA) was performed on the biochemical and anthropometric data to verify whether the dyslipidemia types share a similar biochemical profile plausible of discrimination in chemometric modeling. To discriminate the types of dyslipidemias based on spectral data, Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA) was used and validated with leave-one-out cross-validation. RESULTS: Although no significant difference was obtained between the types of dyslipidemia and normal subjects by CRP, leptin, and cfDNA, there was a significant difference between normal subjects vs combined hyperlipidemia (CH) + hypercholesterolemia (HCL) + hypertriglyceridemia (HTG) (p < 0.05) by the 1245 cm-1 peak [νas(PO2-)] (possible indication of chronic inflammation by increased cfDNA). The area under the curve of the region between 1770 and 1720 cm-1 was significantly increased for CH in relation to other dyslipidemias and normal subjects. Furthermore, there were significant differences for the main representative peaks of lipids, proteins, carbohydrates, and nucleic acids between the types of dyslipidemias and between the types of dyslipidemias and normal subjects. The OPLS-DA model achieved 100 % accuracy with 1 latent variable and Standard Error of Cross-Validation (SECV) < 0.004 for all types of dyslipidemia  and the control group. CONCLUSIONS: Our results suggest that ATR-FTIR spectroscopy associated with chemometric modeling is a plausible applicant for screening the types of dyslipidemias. However, more extensive studies should be conducted to verify the real applicability in clinical analysis laboratories or medical clinics.

Assuntos

RESUMO

O trabalho foi desenvolvido em duas etapas: a produção de mudas de couve-chinesa em bandejas e posterior transplante para o campo, objetivando avaliar o desempenho de diferentes substratos sobre o desenvolvimento da cultura. Para isso determinou-se, tanto na fase de formação das mudas, quanto nas plantas adultas, o comprimento da parte aérea, número de folhas, comprimento da raiz, massa seca da parte aérea, massa seca da raiz e diâmetro do coleto. A etapa de produção de mudas foi conduzida em ambiente protegido, com os seguintes tratamentos: T0 substrato comercial Plantmax® (HA); T1: 100% composto; T2: 95% composto + 2,5% areia + 2,5% pó de rocha; T3: 90% composto + 3% areia + 7% de pó de rocha e T4: 85% composto + 6% areia + 9% pó de rocha. Na produção de mudas, os substratos orgânicos, formulados com 100% e 85% composto, apresentaram melhores resultados, aos 15 e 28 DAS, respectivamente. Os resultados de campo demonstraram que adição de pó de rocha é um fator determinante no desempenho das mudas, sendo os melhores resultados obtidos nas parcelas cultivadas com mudas obtidas nos substratos formulados com 7 e 9% de pó de basalto.

The research was carried out in two stages: the production of seedlings of chinese cabbage in trays with subsequent transplantation for the field, aiming to evaluate the performance of different substrates on the culture development. For this reason it was determined, both at the stage of formation of the seedlings, as in adult plants the length of the aerial part, number of leaves, root length, dry mass of the aerial part, root dry mass and diameter of the root collar. The experiment was conducted in a protected environment with the following treatments: T0 Plantmax substrate® (HA); T1: 100% compound; T2: 95% compost + 2.5% sand + 2.5% rock powder; T3: 90% compost + 3% sand + 7% powdered rock and T4: 85% compost + 6% sand + 9% rock powder. In the production of seedlings, the organic substrates formulated with 100% and 85% compound, had better results, the 15 and 28, respectively. The results of field have shown that the addition of rock powder is a determinant factor in the performance of seedlings, with the best results in the plots cultivated with seedlings obtained in the substrates formulated with 7 and 9% of basalt powder.

RESUMO

The research was carried out in two stages: the production of seedlings of chinese cabbage in trays with subsequent transplantation for the field, aiming to evaluate the performance of different substrates on the culture development. For this reason it was determined, both at the stage of formation of the seedlings, as in adult plants the length of the aerial part, number of leaves, root length, dry mass of the aerial part, root dry mass and diameter of the root collar. The experiment was conducted in a protected environment with the following treatments: T0 Plantmax substrate® (HA); T1: 100% compound; T2: 95% compost + 2.5% sand + 2.5% rock powder; T3: 90% compost + 3% sand + 7% powdered rock and T4: 85% compost + 6% sand + 9% rock powder. In the production of seedlings, the organic substrates formulated with 100% and 85% compound, had better results, the 15 and 28, respectively. The results of field have shown that the addition of rock powder is a determinant factor in the performance of seedlings, with the best results in the plots cultivated with seedlings obtained in the substrates formulated with 7 and 9% of basalt powder.

O trabalho foi desenvolvido em duas etapas: a produção de mudas de couve-chinesa em bandejas e posterior transplante para o campo, objetivando avaliar o desempenho de diferentes substratos sobre o desenvolvimento da cultura. Para isso determinou-se, tanto na fase de formação das mudas, quanto nas plantas adultas, o comprimento da parte aérea, número de folhas, comprimento da raiz, massa seca da parte aérea, massa seca da raiz e diâmetro do coleto. A etapa de produção de mudas foi conduzida em ambiente protegido, com os seguintes tratamentos: T0 substrato comercial Plantmax® (HA); T1: 100% composto; T2: 95% composto + 2,5% areia + 2,5% pó de rocha; T3: 90% composto + 3% areia + 7% de pó de rocha e T4: 85% composto + 6% areia + 9% pó de rocha. Na produção de mudas, os substratos orgânicos, formulados com 100% e 85% composto, apresentaram melhores resultados, aos 15 e 28 DAS, respectivamente. Os resultados de campo demonstraram que adição de pó de rocha é um fator determinante no desempenho das mudas, sendo os melhores resultados obtidos nas parcelas cultivadas com mudas obtidas nos substratos formulados com 7 e 9% de pó de basalto.

RESUMO

The research was carried out in two stages: the production of seedlings of chinese cabbage in trays with subsequent transplantation for the field, aiming to evaluate the performance of different substrates on the culture development. For this reason it was determined, both at the stage of formation of the seedlings, as in adult plants the length of the aerial part, number of leaves, root length, dry mass of the aerial part, root dry mass and diameter of the root collar. The experiment was conducted in a protected environment with the following treatments: T0 Plantmax substrate® (HA); T1: 100% compound; T2: 95% compost + 2.5% sand + 2.5% rock powder; T3: 90% compost + 3% sand + 7% powdered rock and T4: 85% compost + 6% sand + 9% rock powder. In the production of seedlings, the organic substrates formulated with 100% and 85% compound, had better results, the 15 and 28, respectively. The results of field have shown that the addition of rock powder is a determinant factor in the performance of seedlings, with the best results in the plots cultivated with seedlings obtained in the substrates formulated with 7 and 9% of basalt powder.

O trabalho foi desenvolvido em duas etapas: a produção de mudas de couve-chinesa em bandejas e posterior transplante para o campo, objetivando avaliar o desempenho de diferentes substratos sobre o desenvolvimento da cultura. Para isso determinou-se, tanto na fase de formação das mudas, quanto nas plantas adultas, o comprimento da parte aérea, número de folhas, comprimento da raiz, massa seca da parte aérea, massa seca da raiz e diâmetro do coleto. A etapa de produção de mudas foi conduzida em ambiente protegido, com os seguintes tratamentos: T0 substrato comercial Plantmax® (HA); T1: 100% composto; T2: 95% composto + 2,5% areia + 2,5% pó de rocha; T3: 90% composto + 3% areia + 7% de pó de rocha e T4: 85% composto + 6% areia + 9% pó de rocha. Na produção de mudas, os substratos orgânicos, formulados com 100% e 85% composto, apresentaram melhores resultados, aos 15 e 28 DAS, respectivamente. Os resultados de campo demonstraram que adição de pó de rocha é um fator determinante no desempenho das mudas, sendo os melhores resultados obtidos nas parcelas cultivadas com mudas obtidas nos substratos formulados com 7 e 9% de pó de basalto.

RESUMO

The research was carried out in two stages: the production of seedlings of chinese cabbage in trays with subsequent transplantation for the field, aiming to evaluate the performance of different substrates on the culture development. For this reason it was determined, both at the stage of formation of the seedlings, as in adult plants the length of the aerial part, number of leaves, root length, dry mass of the aerial part, root dry mass and diameter of the root collar. The experiment was conducted in a protected environment with the following treatments: T0 Plantmax substrate® (HA); T1: 100% compound; T2: 95% compost + 2.5% sand + 2.5% rock powder; T3: 90% compost + 3% sand + 7% powdered rock and T4: 85% compost + 6% sand + 9% rock powder. In the production of seedlings, the organic substrates formulated with 100% and 85% compound, had better results, the 15 and 28, respectively. The results of field have shown that the addition of rock powder is a determinant factor in the performance of seedlings, with the best results in the plots cultivated with seedlings obtained in the substrates formulated with 7 and 9% of basalt powder.

O trabalho foi desenvolvido em duas etapas: a produção de mudas de couve-chinesa em bandejas e posterior transplante para o campo, objetivando avaliar o desempenho de diferentes substratos sobre o desenvolvimento da cultura. Para isso determinou-se, tanto na fase de formação das mudas, quanto nas plantas adultas, o comprimento da parte aérea, número de folhas, comprimento da raiz, massa seca da parte aérea, massa seca da raiz e diâmetro do coleto. A etapa de produção de mudas foi conduzida em ambiente protegido, com os seguintes tratamentos: T0 substrato comercial Plantmax® (HA); T1: 100% composto; T2: 95% composto + 2,5% areia + 2,5% pó de rocha; T3: 90% composto + 3% areia + 7% de pó de rocha e T4: 85% composto + 6% areia + 9% pó de rocha. Na produção de mudas, os substratos orgânicos, formulados com 100% e 85% composto, apresentaram melhores resultados, aos 15 e 28 DAS, respectivamente. Os resultados de campo demonstraram que adição de pó de rocha é um fator determinante no desempenho das mudas, sendo os melhores resultados obtidos nas parcelas cultivadas com mudas obtidas nos substratos formulados com 7 e 9% de pó de basalto.

RESUMO

Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine disorder with individual susceptibility determined by genetic and environmental risk factors. Recently, studies have evaluated the CAPN10 gene in PCOS patients, suggesting that different alleles may play a role in PCOS susceptibility. We performed a cross-sectional study with 88 southern Brazilian hirsute patients with PCOS or idiopathic hirsutism (IH) to assess the influence of CAPN10 genetic variants on clinical and biochemical features of metabolic syndrome. PCOS patients were defined by oligo/amenorrheic cycles (<9 cycles/year), increased levels of serum testosterone and/or free androgen index, and exclusion of other disorders associated with hyperandrogenism. IH was diagnosed in hirsute patients with regular ovulatory cycles (luteal-phase progesterone levels >3.8 ng/ml), normal androgen levels, and without any known underlying disease (n = 29). Metabolic syndrome was defined according to the 2001 criteria of the National Cholesterol Education Program, Adult Treatment Panel III. UCSNP-43 polymorphism of CAPN10 was related to metabolic syndrome (p = 0.047) in PCOS; UCSNP-19 and UCSNP-63 were not associated with phenotypic traits in PCOS. These results provide evidence that CAPN10 gene UCSNP-43 polymorphisms may influence the PCOS metabolic phenotype. This should be further confirmed in large population-based studies.

Assuntos