RESUMO
ODF1 has been described as an exclusively expressed testicular protein and is located in the outer dense fibers along the sperm tail. ODF1 has been involved in the sperm motility and in the development of the flagellum, but the function of ODF1 is not already clear. Other ODF proteins, such as ODF2 have been characterized in other tissues like the basal body of the kidney primary cilium, but so far only the mRNA of ODF1 has been described in other tissues. These observations let us to hypothesize that the expression of the protein ODF1 could not be limited to the testis. Therefore, in the present work we proposed to evaluate if the ODF1 protein could also be present in tissues other than the testis. Here we demonstrated through western blot, immunofluorescence, and RT-PCR techniques that the protein and mRNA of ODF1 have been identified in the rat kidney. Finally, the presence of ODF1 in kidney has also been confirmed through proteomic analysis using mass spectrometry. The results derived from these different complementary approaches indicate that, to our knowledge and for the first time, ODF1 is demonstrated to be present in an additional organ different to testis. This results raise new questions about potential other functions and locations of the ODF1 protein.
RESUMO
A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather in a mouse model a chronic feeding with a diet supplemented with chicken fat affects sperm head morphology. To accomplish this, we fed mice for 16 weeks with either control food (low-fat diet, LFD) or control food supplemented with 22% chicken fat (high-fat diet, HFD). At the end of the feeding regimen, we measured: redox and inflammatory changes, cholesterol accumulation in testis and analyzed testicular morphological structure and ultra-structure and liver morphology. We found that the mice fed HFD resembled some features of the human metabolic syndrome, including systemic oxidative stress and inflammation, this group showed an increment in the following parameters; central adiposity (adiposity index: 1.07 ± 0.10 vs 2.26 ± 0.17), dyslipidemia (total cholesterol: 153.3 ± 2.6 vs 175.1 ± 8.08 mg/dL), insulin resistance (indirect Insulin resistance index, TG/HDL-c: 2.94 ± 0.33 vs 3.68 ± 0.15) and fatty liver. Increased cholesterol content measured by filipin was found in the testicles from HFD (fluorescence intensity increase to 50%), as well as an alteration of spermiogenesis. Most remarkably, a disorganized manchette-perinuclear ring complex and an altered morphology of the sperm head were observed in the spermatozoa of HFD-fed mice. These results add new information to our understanding about the mechanisms by which systemic oxidative stress and inflammation may influence sperm-head morphology and indirectly male fertility.
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The objective of this study was to estimate the relative contribution of factors affecting how quickly cattle become pregnant in Argentine dairy herds. Data from 76,401 cows from 249 dairy herds were analyzed. A hazard model was used to explore days open (DO). The factors considered were milk yield, lactation number, calving season, and breeding technique (i.e., type of service: artificial insemination [AI], or combined service). Cows with lower milk yield had 1.09 to 1.38 higher likelihood to become pregnant than those with higher milk yield (P < 0.0001). The number of DO increased linearly with an increasing number of lactations (P < 0.0001). Cows calving in fall-winter had a shorter interval to conception than those calving in summer. The hazard rate for combined service was 1.27; therefore, cows with combined service were more likely to become pregnant during the observation period than those bred by AI. The difference in DO between cows of high versus low milk yield was smaller when dairies used AI as the main breeding technique than when they used combined service. Furthermore, dairies using mainly combined service had lower milk yield (5693.7 L) than those using mainly AI (7684.4 L). Although lactation number and calving season contributed to explain the number of DO, the influence of production level, the type of service, and the interaction between them was also associated with reproductive efficiency in Argentine dairy herds.
Assuntos
Cruzamento/métodos , Bovinos/fisiologia , Animais , Argentina , Indústria de Laticínios , Feminino , Fertilidade , Fertilização , Inseminação Artificial/veterinária , Lactação , Gravidez , Modelos de Riscos Proporcionais , Estações do Ano , Fatores de TempoRESUMO
Necrolytic migratory erythema (NME) is a cutaneous manifestation of the glucagonoma syndrome. We present a case with a pancreatic glucagon-secreting tumour, skin eruption and a good response to treatment.
Assuntos
Exantema/etiologia , Glucagonoma/diagnóstico , Neoplasias Pancreáticas/diagnóstico , Diagnóstico Diferencial , Exantema/patologia , Glucagon/sangue , Glucagonoma/sangue , Glucagonoma/complicações , Glucagonoma/diagnóstico por imagem , Glucagonoma/patologia , Glucagonoma/cirurgia , Humanos , Perna (Membro) , Masculino , Pessoa de Meia-Idade , Pancreatectomia , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/complicações , Neoplasias Pancreáticas/diagnóstico por imagem , Neoplasias Pancreáticas/patologia , Neoplasias Pancreáticas/cirurgia , Esplenectomia , Tomografia Computadorizada por Raios XRESUMO
INTRODUCTION: Acute peripheral neuropathy represents a medical emergency. The causes of it are diverse and plentiful. The most common cause of acute paralytic peripheral neuropathy is the Guillain-Barré syndrome (GBS). As many as 85% of those affected can be expected to make an excellent recovery. OBJECTIVE: To describe the principal risk factors associated, clinical manifestations, treatment, evolution and complications of 28 cases of Guillain-Barré syndrome (GBS) in the "Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán". METHODS: We search in our computer records all files under diagnostic of GBS, during the last ten years. We found 28 cases that were available to study. RESULTS: Mean age was 37 years old (SD 17.2). Fifteen patients were female (54%) and 13 were male (46%). Nine patients (32%) were preceded by a superior via infection, 5 (18%) by a diarrhea illness and 14 patients had not a predisposing factor. The duration of symptoms before diagnostic has a median of 7 days (2-15). Twenty-six patients (93%) had an ascending paralysis and 18 had paresthesias (64%). The most frequent subtype was acute inflammatory-demyelinating polyneuropathy (AIDP) in 18 patients (64%), acute motor-sensory axonal neuropathy (AMSAN) in 5 (18%), acute motor axonal neuropathy (AMAN) in 3 (11%) and 2 patients (7%) had the Fisher-Miller syndrome. Fifteen patients (54%) developed respiratory involvement requiring mechanical ventilation. Twenty-four patients (86%) had cerebrospinal fluid proteins elevated. Twenty patients (72%) had a total recovery, 6 (21%) had a partial recovery and 2 had not any response (7%). DISCUSSION: GBS is a particularly highstakes illness in that its onset is sudden and paralysis is frequently extreme (requiring assisted respiration), however, as many as 85% of those affected can be expected to make an excellent recovery. In our study the majority of patients (54%) develop respiratory involvement requiring mechanical ventilation but in this group the majority had a favorable outcome (71%).
Assuntos
Síndrome de Guillain-Barré , Adulto , Feminino , Síndrome de Guillain-Barré/diagnóstico , Síndrome de Guillain-Barré/epidemiologia , Síndrome de Guillain-Barré/terapia , Humanos , Masculino , Fatores de RiscoAssuntos
2-Amino-5-fosfonovalerato/análogos & derivados , Aprendizagem da Esquiva/fisiologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Núcleo Accumbens/fisiologia , Receptores de N-Metil-D-Aspartato/fisiologia , 2-Amino-5-fosfonovalerato/administração & dosagem , 2-Amino-5-fosfonovalerato/farmacologia , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Antagonistas de Aminoácidos Excitatórios/administração & dosagem , Masculino , Microinjeções , Núcleo Accumbens/efeitos dos fármacos , Ratos , Receptores de N-Metil-D-Aspartato/antagonistas & inibidoresRESUMO
Describe las interrelaciones que existen entre las distintas partes del sistema de manejo de residuos sólidos: generación, almacenamiento, recolección, procesamiento, reciclaje, transporte y disposición final; considerando los factores de cantidad, composición y variación estacional que determinan los métodos a seguirse según la región. Así mismo se dan las pautas para determinar cuales son las características predominantes en los residuos generados en el área donde se va a emplazar el centro de disposición de residuos sólidos, además de las características concernientes al transporte y estaciones de transferencia
Assuntos
Administração Municipal , Resíduos SólidosRESUMO
Describe las interrelaciones que existen entre las distintas partes del sistema de manejo de residuos sólidos: generación, almacenamiento, recolección, procesamiento, reciclaje, transporte y disposición final; considerando los factores de cantidad, composición y variación estacional que determinan los métodos a seguirse según la región. Así mismo se dan las pautas para determinar cuales son las características predominantes en los residuos generados en el área donde se va a emplazar el centro de disposición de residuos sólidos, además de las características concernientes al transporte y estaciones de transferencia