RESUMO
BACKGROUND: Oxidative modifications have been observed in lipids and proteins in lipoproteins isolated from women with preeclampsia. Thus, newborns could also be susceptible to this damage directly through their mothers. In this study, we evaluated the oxidative profile of LDL-c and HDL-c lipoproteins isolated from the umbilical cord from newborns born to women with preeclampsia. METHODS: Thirty newborns born to women with preeclampsia and thirty newborns born to women with healthy pregnancies were included. Lipid-damage biomarkers, including conjugated dienes, lipohydroperoxides and malondialdehyde, were measured. The reduction of nitroblue tetrazolium, formation of dityrosines, and carbonylation of proteins were assessed as indicators of protein damage. The protective activity of paraoxonase-I on HDL-c particles was evaluated. The total antioxidant capacity and lipid profiles were quantified in plasma. Data were analysed using Student's t-tests and Pearson correlation coefficients. RESULTS: Compared with the control group, the preeclampsia group had an increase in the percentage of lipid damage in both lipoproteins. There was an increase of 23.3 and 19.9% for conjugated dienes, 82.4 and 21.1% for lipohydroperoxides, and 103.8 and 51.5% for malondialdehyde in LDL-c and HDL-c, respectively. However, these infants did not show evident damage in protein oxidation. The activity of the enzyme paraoxonase-I was decreased by 36.2%; by contrast, the total antioxidant capacity was increased by 40% (protein) and 28.8% (non-protein). CONCLUSIONS: The oxidative modifications that occur in HDL-c and LDL-c isolated from newborns from women with preeclampsia are mainly caused by lipoperoxidation processes related to evident paraoxonase-I inactivation. The absence of protein damage is likely linked to an increase in total antioxidant capacity. Therefore, antioxidant support could be helpful in reducing oxidative stress in mother/newborn dyads.
Assuntos
HDL-Colesterol/sangue , LDL-Colesterol/sangue , Lipoproteínas HDL/sangue , Pré-Eclâmpsia/sangue , Adulto , Antioxidantes/metabolismo , Biomarcadores/sangue , Feminino , Sangue Fetal , Feto/metabolismo , Humanos , Recém-Nascido , Peroxidação de Lipídeos/genética , Lipídeos/sangue , Malondialdeído/metabolismo , Oxirredução , Estresse Oxidativo/genética , Pré-Eclâmpsia/patologia , Gravidez , Triglicerídeos/sangueRESUMO
BACKGROUND: Oxidative stress causes biochemical changes in lipids and proteins; these changes can induce damage to the vascular endothelium and create maternal complications that are characteristic of preeclampsia. In this study, we evaluated the oxidative profile of lipoproteins isolated from women with preeclampsia. METHODS: Thirty women diagnosed with preeclampsia and thirty women without preeclampsia were included in the study. Lipid-damage biomarkers, including conjugated dienes, lipohydroperoxides and malondialdehyde, were measured. The reduction of nitroblue tetrazolium, the formation of dityrosines, and the carbonylation of proteins were assessed as indicators of protein damage. The protective activity of HDL-c was evaluated by the paraoxonase-I activity present on the HDL-c particles. Serum lipid profiles were also quantified in both groups. Data were analysed using Student's t test and the Pearson correlation coefficient. RESULTS: Our results demonstrated in PE women evident oxidative changes in the lipids and proteins in HDL-c and LDL-c particles and the activity of the antioxidant enzyme PON-I decreased 59.9%. HDL-c exhibited self-defence, as demonstrated by the negative correlation between paraoxonase-I activity and the formation of lipohydroperoxides in HDL-c (r = -0.3755, p < 0.005). CONCLUSIONS: LDL-c and HDL-c isolated from women with preeclampsia show oxidative damage to lipids and proteins. We propose an oxidative profile based on the oxidation levels indicated by each of the markers used. We also found that paraoxonase-I is inactivated in the presence of lipohydroperoxides. Antioxidant support might be helpful to reduce oxidative stress in patients with preeclampsia. Further investigations are necessary to define the association between antioxidant activities and preeclampsia.
Assuntos
Lipoproteínas HDL/sangue , Lipoproteínas LDL/sangue , Pré-Eclâmpsia/sangue , Pré-Eclâmpsia/metabolismo , Adulto , Antioxidantes/metabolismo , Biomarcadores/sangue , Biomarcadores/metabolismo , Feminino , Humanos , Masculino , Malondialdeído/sangue , Malondialdeído/metabolismo , Oxirredução , Estresse Oxidativo/fisiologia , Gravidez , Triglicerídeos/sangueRESUMO
Bacterial vaginosis is one of the most frequent infections during the woman's reproductive age. Lactobacilli normal flora is substituted by relatively elevated Gardnerella vaginalis (GV), anaerobic bacteroids, Mobiluncus and Mycoplasma. The purpose of this study is to perform a morphological analysis of possible mechanisms for adhesion and penetration of GV in the heterosexual couple, in squamous epithelium at vaginal wall, as well as in seminal fluid. Ten couples with positive GV culture with three to four days of abstinence, were studied. The women presented with at least three of the four Amsel's criteria. Samples were obtained from vaginal lateral walls and from seminal fluid; these were divided in two parts: 1. To realize cultures for GV. Ureaplasma urealyticum and Mycoplasma hominis; and 2. For an ultrastructural analysis. Samples were processed with common techniques for electronic microscopy. In vaginal cells, bacteria similar to GV free form fixed to plasmatic membrane and inside the cellular cytoplasm. In the seminal fluid there were found numerous urethral cells of desquamation, which presented, as well as in woman, free form bacteria, fixed to plasmatic membrane and inside the cytoplasm. In four cases, bacteria similar to Mycoplasma, were found; and one case with particles suggesting cytomegalovirus. It is concluded in this study that: 1. The male presents with urethral cells similar to vaginal "guide cells". 2. GV colonizes urethral epithelium in the male. 3. Male is capable of infecting and/or re-infecting the woman.